Glycoprotein B subtyping of cytomegalovirus (CMV) in the vitreous of patients with AIDS and CMV retinitis.
J Infect Dis
; 178(4): 1149-53, 1998 Oct.
Article
em En
| MEDLINE
| ID: mdl-9806048
ABSTRACT
A 550-bp region of the cytomegalovirus (CMV) glycoprotein B (gB) gene was amplified by polymerase chain reaction (PCR) from 141 vitreous specimens of 120 patients with AIDS and CMV retinitis from three different metropolitan centers. The distribution of gB subtypes I, II, III, and IV were 19%, 43%, 12%, and 21%, respectively, based on restriction enzyme digestion patterns of PCR-amplified DNA. Two patients had simultaneous infection with two different gB subtypes. The ratio of gB subtypes was similar among the three geographically distinct patient populations. Two of 14 patients with bilateral vitreous specimens had different viral subtypes in each eye. In addition, different gB subtypes were observed in 1 of 6 patients with serial specimens. The ratio of different gB subtypes in the vitreous of patients with CMV retinitis is similar to that previously reported in the peripheral blood of patients with advanced AIDS.
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Bases de dados:
MEDLINE
Assunto principal:
Corpo Vítreo
/
Proteínas do Envelope Viral
/
Síndrome da Imunodeficiência Adquirida
/
Retinite por Citomegalovirus
/
Citomegalovirus
Tipo de estudo:
Clinical_trials
Limite:
Humans
País/Região como assunto:
America do norte
Idioma:
En
Revista:
J Infect Dis
Ano de publicação:
1998
Tipo de documento:
Article
País de afiliação:
Estados Unidos