RESUMO
The relationship between essential fatty acid (EFA) status and degree of hyperbilirubinaemia and oxidant stress in infants and children with chronic liver diseases was evaluated. Thirty patients with chronic cholestasis and 30 with liver cirrhosis were examined; 30 healthy subjects served as controls. Patient groups had significant decreases in EFAs and significant elevation of total bilirubin. Levels of thiobarbituric acid reactive substances were significantly raised and were significantly inversely correlated to decreased EFA levels. There were also significant decreases in retinol, alpha-tocopherol and alpha-tocopherol/total lipids ratio, which had significant positive correlations with decreased EFA levels. Infants and children with chronic liver diseases have a high risk of EFA deficiency correlated with progressive elevation of serum bilirubin and progressive deterioration of oxidant status.
Assuntos
Colestase/metabolismo , Ácidos Graxos Essenciais , Cirrose Hepática/metabolismo , Adolescente , Atresia Biliar/complicações , Bilirrubina/sangue , Estudos de Casos e Controles , Criança , Pré-Escolar , Colestase/etiologia , Doença Crônica , Egito , Ácidos Graxos Essenciais/sangue , Ácidos Graxos Essenciais/deficiência , Feminino , Doença de Depósito de Glicogênio Tipo III/complicações , Hepatopatia Veno-Oclusiva/complicações , Hepatite Crônica/complicações , Degeneração Hepatolenticular/complicações , Humanos , Hiperbilirrubinemia/sangue , Hiperbilirrubinemia/etiologia , Cirrose Hepática/etiologia , Masculino , Estresse Oxidativo/fisiologia , Veia Porta , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Trombose Venosa/complicações , Vitamina A/sangue , Deficiência de alfa 1-Antitripsina/complicações , alfa-Tocoferol/sangueRESUMO
The ratio of stearic to oleic acids, i.e. the fatty acid saturation index, in red blood cell membranes was assayed in 60 patients with chronic hepatitis C virus infection before and after interferon-alpha therapy. Results were compared with 20 healthy controls. Hepatitis C virus titre was also assayed before and after interferon-alpha therapy. Within 2-5 months following interferon-alpha therapy, a significant inverse correlation was observed between saturation index and hepatitis C virus load. We conclude that hepatitis C virus infection enhances the degree of desaturation of 18-carbon fatty acids and that interferon-alpha is involved in their metabolism by increasing the degree of saturation and subsequent decrease in membrane fluidity.
Assuntos
Membrana Eritrocítica , Hepatite C Crônica/tratamento farmacológico , Hepatite C Crônica/metabolismo , Interferon-alfa/uso terapêutico , Ácidos Oleicos , Ácidos Esteáricos , Antivirais/uso terapêutico , Biomarcadores , Estudos de Casos e Controles , Cromatografia , Quimioterapia Combinada , Membrana Eritrocítica/química , Membrana Eritrocítica/efeitos dos fármacos , Hepatite C Crônica/virologia , Humanos , Interferon-alfa/farmacologia , Fluidez de Membrana/efeitos dos fármacos , Fluidez de Membrana/fisiologia , Ácidos Oleicos/análise , Ácidos Oleicos/metabolismo , Valor Preditivo dos Testes , Prognóstico , Ribavirina/uso terapêutico , Índice de Gravidade de Doença , Ácidos Esteáricos/análise , Ácidos Esteáricos/metabolismo , Estearoil-CoA Dessaturase/efeitos dos fármacos , Estearoil-CoA Dessaturase/metabolismo , Fatores de Tempo , Resultado do Tratamento , Carga ViralRESUMO
As part of an investigation of the possible role of autoimmune mechanisms in the development of chronic active hepatitis (CAH) in schistosomiasis, sera from 25 patients (Group I) with hepatic schistosomiasis without histological features of CAH and 5 patients (Group II) with CAH and intestinal mansoniasis, but without discernible hepatic infection, were examined for the presence of the liver membrane antibody (LMA) that is reportedly associated with the autoimmune variant of CAH. All patients were HBsAg-negative. Circulating LMA was found in 17 (68%) Group I and 3 (60%) Group II patients. Low titres of various organ-non-specific autoantibodies were found in about a third of all patients but these did not correlate with the presence of LMA. There was also no correlation between LMA and antibodies to the hepatitis B core antigen (anti-HBc), found in the sera of 67% of the patients, indicating that occult or recent past hepatitis B virus infection was not a factor in LMA production. Long-term follow-up of the Group I patients should reveal whether current LMA-positivity is related to eventual development of CAH in hepatic schistosomiasis.
Assuntos
Hepatite Crônica/complicações , Hepatopatias Parasitárias/complicações , Esquistossomose mansoni/complicações , Adolescente , Adulto , Criança , Egito , Seguimentos , Humanos , Masculino , Pessoa de Meia-IdadeAssuntos
Abdome/diagnóstico por imagem , Esquistossomose mansoni/diagnóstico por imagem , Adolescente , Criança , Árvores de Decisões , Egito/epidemiologia , Humanos , Fígado/parasitologia , Sistema Porta/parasitologia , Esquistossomose mansoni/epidemiologia , Esquistossomose mansoni/parasitologia , UltrassonografiaAssuntos
L-Lactato Desidrogenase/urina , Esquistossomose/complicações , Neoplasias da Bexiga Urinária/diagnóstico , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Esquistossomose/enzimologia , Neoplasias da Bexiga Urinária/enzimologia , Neoplasias da Bexiga Urinária/etiologia , Neoplasias da Bexiga Urinária/urinaRESUMO
The relationship between essential fatty acid [EFA] status and degree of hyperbilirubinaemia and oxidant stress in infants and children with chronic liver diseases was evaluated. Thirty patients with chronic cholestasis and 30 with liver cirrhosis were examined; 30 healthy subjects served as controls. Patient groups had significant decreases in EFAs and significant elevation of total bilirubin. Levels of thiobarbituric acid reactive substances were significantly raised and were significantly inversely correlated to decreased EFA levels. There were also significant decreases in retinol, alpha-tocopherol and alpha-tocopherol/total lipids ratio, which had significant positive correlations with decreased EFA levels. Infants and children with chronic liver diseases have a high risk of EFA deficiency correlated with progressive elevation of serum bilirubin and progressive deterioration of oxidant status
Assuntos
Atresia Biliar , Bilirrubina , Estudos de Casos e Controles , Pré-Escolar , Doença Crônica , Ácidos Graxos Essenciais , Doença de Depósito de Glicogênio Tipo III , Hepatopatia Veno-Oclusiva , Hiperbilirrubinemia , Estresse Oxidativo , ColestaseRESUMO
The ratio of stearic to oleic acids, i.e. the fatty acid saturation index, in red blood cell membranes was assayed in 60 patients with chronic hepatitis C virus infection before and after interferon-alpha therapy. Results were compared with 20 healthy controls. Hepatitis C virus titre was also assayed before and after interferon-alpha therapy. Within 2-5 months following interferon-alpha therapy, a significant inverse correlation was observed between saturation index and hepatitis C virus load. We conclude that hepatitis C virus infection enhances the degree of desaturation of 18-carbon fatty acids and that interferon-alpha is involved in their metabolism by increasing the degree of saturation and subsequent decrease in membrane fluidity