Physicochemical and microbiological parameters of rumen fluid of lactating goats fed diets containing crude glycerin from waste frying oil used in biodiesel production.

Crude glycerin from the biodiesel industry is a alternative energy source used to replace part of the energy components of the diet, since the nutritional value of glycerol makes it suitable for use in ruminant diets. The objective was to analyze the effects including crude glycerin (CG) derived from the production of biodiesel from residual frying oil, in diets of lactating goats on the physicochemical and microbiological parameters of rumen fluid. Eight 2-year-old goats weighting approximately 42.06 ± 3.5 kg of body weight (BW) were kept in individual stalls, receiving diets containing 0, 7, 14 and 21% of CG (% DM). The experiment was carried out according to a double 4 × 4 Latin square and lasted for 80 days. Orthogonal contrasts were tested to determine linear and quadratic effects, as well as the effects of 0% CG in the diet in comparison to the other treatments. There was a linear decreasing effect on DM intake (P = 0.001) and neutral detergent fiber (P = 0.0001), as well as a quadratic effect (P = 0.0002) on ether extract (EE) intake with increasing level of CG in the diet. Including CG affected motility and density of the protozoa (P = 0.0001). Treatments had no effect on pH, N-NH3 concentration and percentage of protozoa in the rumen liquid. There was increasing linear effect on sedimentation and flotation time (P = 0.001). Therefore, including 14 and 21% CG in the diet as a source of glycerol decreased nutrient intake and altered the ruminal microbiota, but pH and N-NH3 were not affected. Thus, to avoid impairment to the physicochemical and microbiological parameters of the rumen liquid of goats, the inclusion of CG should not exceed 7% DM.

Physicochemical composition, fatty acid profile and sensory attributes of meat (longissimus lumborum muscle) from Nellore and Nellore-cross bulls.

This study aimed to compare the physicochemical characteristics, fatty acid composition, and sensory attributes of the meat from three genetic groups: Nellore (Nell), ½ Nellore × ½ Angus (NeAn), and » Nellore × » Angus × ½ Senepol (NASe). Longissimus lumborum muscle from 30 slaughtered bulls with a body weight of 549 ± 32.5 kg was used. The water holding capacity was greater for the Nell and NeAn groups than for the NASe group. Meat samples from the NASe group exhibited a higher L* index than those from the NeAn group and lower a* and b* color indexes than those from the Nell group. The meat fatty acid profiles showed that the Nell group had higher concentrations of 12:0, 14:0, 18:1 t11, 14:1 c9, 16:1 c9, 18:1 c9, 18:1 c11, 18:2 c9, t11 (conjugated linoleic acid (CLA)), and 20:3 n-6 polyunsaturated fatty acid (PUFA) than the NeAn and NASe groups. The total saturated (ΣSFA), unsaturated (ΣUFA), and monounsaturated (ΣMUFA) fatty acid concentrations were higher and the ΣPUFA:ΣSFA ratio was lower in the Nell group than in the NeAn group. The Δ9-desaturase C16 activity was significantly higher in the Nell and NASe groups than in the NeAn group. The atherogenicity index (AI) tended to be lower in the crossbreeds than in the Nell breed. The NASe group presented meat with better tenderness, juiciness, and overall acceptance than the Nell and NeAn groups and was therefore the best genetic group for beef production of the tested groups.

Superabsorbent Hydrogels Based to Polyacrylamide/Cashew Tree Gum for the Controlled Release of Water and Plant Nutrients.

Agricultural production is influenced by the water content in the soil and availability of fertilizers. Thus, superabsorbent hydrogels, based on polyacrylamide, natural cashew tree gum (CG) and potassium hydrogen phosphate (PHP), as fertilizer and water releaser were developed. The structure, morphology, thermal stability and chemical composition of samples of polyacrylamide and cashew tree gum hydrogels with the presence of fertilizer (HCGP) and without fertilizer (HCG) were investigated, using X-ray diffractometry (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscopy (SEM), Thermogravimetric Analysis (TGA/DTG) and Energy Dispersive Spectroscopy (EDS). Swelling/reswelling tests, textural analysis, effect of pH, release of nutrients and kinetics were determined; the ecotoxicity of the hydrogels was investigated by the Artemia salina test. The results showed that PHP incorporation in the hydrogel favored the crosslinking of chains. This increased the thermal stability in HCGP but decreased the hardness and adhesion properties. The HCGP demonstrated good swelling capacity (~15,000 times) and an excellent potential for reuse after fifty-five consecutive cycles. The swelling was favored in an alkaline pH due to the ionization of hydrophilic groups. The sustained release of phosphorus in HCGP was described by the Korsmeyer-Peppas model, and Fickian diffusion is the main fertilizer release mechanism. Finally, the hydrogels do not demonstrate toxicity, and HCGP has potential for application in agriculture.

Effect of Edible Onion (Allium cepa L.) Film on Quality, Sensory Properties and Shelf Life of Beef Burger Patties.

The production of edible film from onion (Allium cepa L.) to be applied as packaging is attractive, due to its chemical properties and biodegradable characteristics. Thus, we tested the hypothesis that edible onion film can positively influence the sensory properties, quality and increasing shelf life of beef burgers patties. The experiment was designed in a 4 × 2 factorial scheme, with two treatments (beef burgers patties with or without edible onion film) at an interval of four storage times (0, 3, 6 and 9 days) at 4 °C. The uncoated burger patties (control) suffered the most intense color modifications during the storage (p < 0.05). The luminosity index was higher (p < 0.05) in the control at all storage times, except at day 6, and redness, yellowness and chrome were higher (p < 0.05) in the edible onion film patties at all storage times. The pH of the beef burger patties was lower (p < 0.05) at all storage times when the edible onion film was applied. For the texture profile, only the chewiness was affected, as the inclusion of the edible onion film improved the chewing of the beef burgers patties over the storage time (p < 0.05). Additionally, there was an inhibition of the microbial growth of mesophiles and psychrophiles with the application of the edible onion film in beef burgers patties. The use of edible onion film improved the perception of panelists for the variables texture, color, flavor, odor and overall appearance, and increased the preference of panelists. The edible onion film is recommended for preserving beef burgers patties, as it delays the proliferation of unwanted microorganisms, stabilizes and improves the color parameters and sensory attributes, and increases the overall acceptance of the consumer.

Effect of different blend levels of spineless cactus and Mombasa hay as roughage on intake, digestibility, ingestive behavior, and performance of lambs.

This study aimed to determine the best level of the blend of spineless cactus (Nopalea cochenillifera) and Mombasa (Panicum maximum) hay as roughage in the diet of lambs based on intake, digestibility, ingestive behavior, and lamb performance. Forty-eight uncastrated crossbred Santa Ines breed lambs with an average age of 4 months old and an average initial BW of 20.5 kg ± 2.8 kg were distributed in two experiments using a completely randomized design: experiment 1: 16 lambs (four treatments and four replicates) for in vivo digestibility trial in metabolic cage; experiment 2: 32 lambs (four treatments and eight replicates) in feedlot to determine intake, ingestive behavior, and performance. As the amount of cactus in the roughage increased, there was a linear reduction (P <0.001) on intake (g/day) of dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), non-fibrous carbohydrates (NFC) digestibility, time spent ruminating, feeding efficiency of NDF (P = 0.0153), NDF rumination efficiency rate (P = 0.032), final BW, TWG, and ADG of lamb. However, there was a linear increase (P <0.001) on the intake of the NFC and TDN, digestibility coefficient of DM, CP, total carbohydrates (TC) and NDF gross energy, metabolizable energy, and time spent idling when spineless cactus increased in the roughage blend. The least amount of cactus in the roughage (112-637 g/kg DM) promoted higher intake of DM and CP, improving lamb performance. However, the blend up to 450-300 g/kg DM improved digestibility and energy production as well as it did not affect the feed efficiency.

Polymorphism of the beta-casein gene and adaptability of Sindhi cows to semiarid conditions.

This study aimed to investigate the effect of beta-casein genotypes (A1A2 and A2A2) in three different thermal comfort conditions on the adaptability of Sindhi cows and as a tool for selecting thermotolerant cattle. Twelve Sindhi cows were used in a completely randomized design in a 2 × 3 factorial arrangement, with six replicates, with two genotypes, and three thermal comfort conditions. The climatic variables were recorded, while black globe temperature, humidity index, and radiant heat load were calculated. We measured respiratory rate, surface temperature, and rectal temperature while the heat tolerance coefficient was calculated. Genotype had no significant effect (p > 0.05) on any of the parameters measured. However, there was a thermal comfort condition effect (p < 0.05) on rectal temperature, surface temperature, and thermal gradients. The respiratory rate and heat tolerance coefficient were not significantly affected (p > 0.05). Therefore, although the results indicate substantial adaptability of Sindhi cows under any thermal conditions, the tested genotypes should not be used as a tool for selecting thermotolerant Sindhi cows.

Chemical composition, fermentative characteristics, and in situ ruminal degradability of elephant grass silage containing Parkia platycephala pod meal and urea.

This study aimed to evaluate the effects of the addition of Parkia platycephala pod meal (PP) and urea on the chemical composition, fermentation characteristics, and in situ ruminal degradability of elephant grass silage. A completely randomized design with a 4 × 2 factorial arrangement was adopted, with four levels of pod meal (0, 10, 20, and 30%) and two levels of urea (0 and 1.5%) on as fed basis. The produced silages were evaluated in terms of dry matter (DM), crude protein (CP), mineral matter (MM), neutral detergent fiber (NDF), acid detergent fiber (ADF), hemicellulose, ammonia nitrogen, pH, short chain fatty acids, and in situ ruminal degradability. For the study of degradation, four Santa Ines rumen fistulated sheep, with average weight of 45 ± 2.5 kg were used. The association of the additives increased (p < 0.05) DM, CP, DM degradability, fractions a, b, and effective degradability at all passage rates (2, 5, and 8%/h) and reduced NDF and ADF contents. DM disappearance increased (p < 0.05) during the incubation time, especially for the silages containing the two additives. The interaction in the rumen environment is essential for microbial multiplication. Thus, the use of additives such as PP and urea contributed to the availability of digestible fractions of the feed and greater use by ruminal microorganisms. The association of Parkia platycephala with urea improves the fermentation characteristics, chemical composition, and degradability of elephant grass silage.

Fatty acid, chemical, and tissue composition of meat comparing Santa Inês breed sheep and Boer crossbreed goats submitted to different supplementation strategies.

To compare the fatty acid (FA) composition, and chemical and tissue composition of meat, 24 uncastrated males, 12 sheep of the Santa Inês breed, and 12 crossbred goats (F1 Boer × undefined breed) with the weight of 24.3 ± 2.38 kg. The animals were distributed in a randomized block design and treatments were arranged in a 2 × 2 factorial design, two small ruminant species/breed (Boer crossbreed goat × Santa Inês breed sheep), and two supplementation strategies (energy × protein energy). There was not an interaction between the small ruminant species and the supplementation on average daily gain, tissue composition, chemical composition, or FA profile of the longissimus lumborum muscle. Sheep presented greater weight and yield of fat in the subcutaneous, intermuscular, and total depots than goats, which results in better finishing of the sheep for slaughter. Goat meat presented a lower lipid concentration than sheep meat. The protein-energy supplementation increased oleic acid, ΣUFA, ΣMUFA, hypocholesterolemic/ Hypercholesterolemic index, and enzymatic activity Δ9-desaturase C18 and decreased SFA capric acid in the muscle when compared with energetic supplementation. In Caatinga biome conditions, Boer crossbreed goats meat has a lower concentration of lipids and a healthier FA composition compared with Santa Inês breed sheep because it has a lower SFA and greater PUFA content, which are sources of n-3 and n-6, which may contribute to the reduction of blood cholesterol (LDL). In addition, protein-energy supplementation also improved the quality of animal fat compared with supplementation only with the energetic concentrate, regardless of species.

Factors affecting occurrence of demodecosis by Demodex bovis in Sindhi cattle (Bos indicus).

The objective was to evaluate the factors affecting the occurrence of bovine demodecosis from the clinical aspects of injuries, blood profile, and the correlation with weight gain and the season of the year in Sindhi-breed animal management in a tropical environment. Thirty-three Sindhi-breed animals were used: 23 females and 10 males. Clinical examination diagnosed that 90% of the examined animals presented skin lesions characteristic of bovine demodecosis caused by Demodex bovis (P < 0.05). The Sindhi presented a higher fecal parasite egg count and showed a lower body weight gain in the rainy season and a greater weight loss in the drought season (P < 0.05), medium injury predominating, with no effect of sex (P > 0.05). During the drought season, animals clinically infected with bovine demodecosis presented lower red blood cell and platelet counts and total plasma protein, and higher mean corpuscular volume and mean corpuscular hemoglobin concentration, a blood count typical of regenerative macrocytic anemia (P < 0.05). During the rainy season of the year (April to June), the animals showed greater weight gain, but a higher fecal parasite egg count with no effect on the incidence of bovine demodecosis. Infection by gastrointestinal nematodes negatively influenced body weight gain and demodecosis in the animals.

Carnauba wax as a wall material for urea microencapsulation.

BACKGROUND: The high ureolytic activity of rumen microbiota is a concern when urea is used in ruminant feed, because it leads to fast urea conversion, resulting in possible intoxication and lower nitrogen utilization. This study intended to microencapsulate urea using carnauba wax to obtain slow-release systems in the rumen. The experiment was conducted in a randomized block design, arranged in a 3 × 2 factorial, with the urea encapsulated with carnauba wax in ratios of 1 : 2; 1 : 3, and 1 : 4 (UME 2; UME 3, and UME 4) and two particles sizes (small, PS ; and large, PL ). RESULTS: All formulations showed excellent properties, including inhibition of urea hygroscopicity. The formulation UME 2 exhibited the greatest yield (91.6%) and microencapsulation efficiency (99.6%) values, whereas the formulation UME 4 presented the greatest thermal stability (259.5 °C) and lowest moisture content (1.81%). The UME 2 formulation presented a slower release than the other UME formulations studied. CONCLUSION: The production of urea microspheres using carnauba wax was successful for all microencapsulated systems developed, evidencing the promising potential for use in ruminant animal diets. The UME 2 formulation with large particles is the most recommended because it permitted greater resistance to microbial attack, allowing a slower release of urea into the rumen, reducing the risk of intoxication or ruminal alkalosis. © 2018 Society of Chemical Industry.

Peanut cake can replace soybean meal in supplements for lactating cows without affecting production.

This study evaluated the total replacement of soybean meal with peanut cake in the concentrate supplement of lactating cows grazing. Eight crossbred 5/8 Holstein × 3/8 Zebu cows between the 45th and 60th day of lactation and 507 ± 35 kg BW were distributed in a 4 × 4 double Latin square design with four levels of peanut cake (0, 110, 220, and 330 g/kg of the dry matter-DM) as a replacement of soybean meal in the concentrate supplement. The intake of pasture, total (pasture + concentrate), crude protein (CP), neutral detergent fiber (NDF), total digestible nutrients, and digestibility of the DM, CP, ether extract (EE), and NDF was not affected by the replacement of soybean meal with peanut cake. However, the EE (P = 0.83) intake and non-fiber carbohydrate digestibility (P = 0.09) exhibited an increasing linear trend by the peanut cake inclusion. The NDF exhibited a decreasing linear trend (P = 0.07) as the level of peanut cake increased. Production, corrected production, and protein, fat, lactose, total solid, and non-fat solid composition concentrations in the cows' milk were not affected (P > 0.05) by the peanut cake inclusion. The protein/fat ratio exhibited an increasing linear trend (P = 0.082) by the peanut cake inclusion. Peanut cake can completely replace soybean meal in the concentrate mixture because it does not alter dry matter intake, production, and composition of milk, and in addition it can reduce the cost of feeding.

Dietary supplementation for Santa Inês hair ewes on pasture at pre- and postpartum periods: dry matter intake, digestibility, milk production, and mineral metabolism.

The aim of this study was to evaluate the impact of different levels of dietary supplementation and reproductive stages on dry matter intake, digestibility, milk production, and mineral metabolism in Santa Inês hair ewes. Two dietary supplement levels of 0.5 and 1.5%, based on body weight, were used. A total of 12 hair ewes (six subjected to 0.5 and six subjected to 1.5% of concentrate supplementation based on body weight-BW) of the Santa Inês breed were evaluated in a completely randomized design with fixed effects of supplementation level, period, and its interactions. Dry matter intake, digestibility, milk production, and mineral metabolism (calcium (Ca), phosphorus (P), magnesium (Mg), alkaline phosphatase (ALP), type I insulin-like growth factor (IGF-I), parathyroid hormone (PTH), and osteocalcin (OC)) were assessed. Dry matter digestibility was affected by the supplementation level (during both pregnancy and lactation), with higher values in ewes fed at a level of 1.5% of BW. A significant interaction between treatment × reproductive stages was found for the Mg concentration. A period effect (P < 0.05) on serum concentrations of P, Ca/P, Mg, and IGF-I was observed. Serum P concentrations were influenced (P < 0.05) by treatments and reproductive stages. There were significant differences in the Ca/P ratio among the reproductive stages. The enzymatic activity of ALP and serum IGFI differed among reproductive stages. Ewes supplemented at a level of 1.5% of BW produced 18.5% more milk than ewes supplemented at a level of 0.5% of BW. The use of 0.5% of body weight in concentrate supplementation is recommended for the reduction of production costs, without having an effect on the mineral metabolism of Santa Inês hair ewes.

Performance and carcass characteristics of lambs fed diets with increasing levels of Mimosa tenuiflora (Willd.) hay replacing Buffel grass hay.

This study evaluated the performance and carcass characteristics of lambs fed diets with increasing levels of Mimosa tenuiflora (Willd.) hay replacing Buffel grass (Cenchrus ciliaris). Twenty-eight Santa Inês male lambs with an average body weight (BW) of 20.3 ± 1.49 kg(mean ± SD) were allocated in individual stalls and distributed in a completely random design with four treatments (0, 20, 40, and 60 g/100 g total DM M. tenuiflora hay replacing Buffel grass hay in diet) with seven replications. M. tenuiflora hay at the level of 20% dry matter (DM) total replacing Buffel grass hay increased final weight (P = 0.006), total weight gain (P < 0.001), average daily weight gain (ADWG; P < 0.001), DM intake (P < 0.001), and feed efficiency (P < 0.001). Intake of crude protein, NDFap, ADFap, ash, ether extract, total and non-fibrous carbohydrates, and total digestible nutrients presented a positive quadratic effect with M. tenuiflora hay replacing Buffel grass hay and 40 g/100 g total DM level presented greater intake. There were positive quadratic effects by M. tenuiflora hay inclusion at 20 g/100 g total DM level on slaughtering weight (P = 0.005), hot carcass weight (P = 0.002), cold carcass weight (P = 0.002), empty body weight (P = 0.001), hot carcass yield (P = 0.002), cold carcass yield (P = 0.003), and increase linear on biological yield (P = 0.003). There was no influence on cooling weight loss (P = 0.284). M. tenuiflora hay may be included in lamb diets at amounts up to 20 g/100 g total DM substitution of Buffel grass hay because increase in the nutrients intake, growth performance, and carcass characteristics.

Fatty acid profiles of milk and Minas frescal cheese from lactating grazed cows supplemented with peanut cake.

Milk and Minas frescal cheese were evaluated from crossbred Holstein × Gir cows that were fed diets enriched with 0, 33, 66 and 100% inclusion levels of palm kernel cake in a concentrated supplement in replace of soybean meal. Eight crossbred lactating cows were distributed (four animals × four treatments × four periods) in the experimental design of double 4 × 4 Latin squares. The capric (C : 10, P = 0.0270), undecylic (C : 11, P = 0.0134), and lauric (C : 12, P = 0.0342) saturated fatty acid concentrations and CLA (C18 : 2c9t11, P = 0.0164) of the milk fat decreased linearly with an increasing percentage of peanut cake in the diet. The increased peanut cake content (100%) in the diet was associated with a linear decrease in C : 10 (P = 0.0447), C : 12 (P = 0.0002), mirystic (C : 14, P 0.05) ratios were not influenced by the different peanut cake levels. The inclusion of up to 100% peanut cake as a substitution for soybean meal in the concentrate of grazing lactating cows resulted in changes in the nutritional quality of their milk products, as indicated by the increase in polyunsaturated fatty acids and the decrease of saturated fatty acids (lauric, myristic, and palmitic).

Intake, Nutrient Apparent Digestibility, and Ruminal Constituents of Crossbred Dorper × Santa Inês Sheep Fed Diets with Babassu Mesocarp Flour.

The aim of this study was to evaluate the effect on intake, apparent digestibility, and ruminal constituents of sheep in response to the addition of increasing levels of babassu mesocarp flour (BMF) to the diet. Twenty crossbred sheep (29.17 ± 2.23 kg) were used in a randomized complete block design. Lambs were confined for 21 days, with 16 days for diet adaptation and 5 days for data collection, in which they were fed an isonitrogenous diet (16.5 ± 0.2 CP, DM basis) containing 70% of concentrate and 30% (DM basis) of Tifton 85 hay. Increasing levels of BMF were 0, 10, 20, and 30% (DM basis). There was a quadratic effect (P < 0.05) on the DM intake, nutrients intake, and digestibility of CP and NFC. The digestibility of DM, OM, TC, and NDF decreased linearly, while EE digestibility increased linearly with increasing levels of BMF. The high NDF content presented in the chemical composition of the babassu mesocarp flour ranked the same as fibrous food, which can limit the inclusion in the diet of high production animals. So, babassu mesocarp flour is an alternative for energy source in lambs feed and can be added at levels up to 10%.

Effect of multiple stress factors (thermal, nutritional and pregnancy type) on adaptive capability of native ewes under semi-arid environment.

This study was conducted to evaluate the effect of multiple stress factors (thermal, nutritional and pregnancy type) on two different native track breeds of ewes as reflected by their adaptive capability under semi-arid environment. The multiple stressor experiment was conducted in twenty-four ewes (12 Santa Inês and 12 Morada Nova ewes). Both heat stress and pregnancy stress was common to all four groups. However, the animals were divided into further two groups within each breed on the basis of nutrition regimen. According the groupings were: Group 1 (Six Santa Ines ewes; heat stress; nutrition at 0.5% of BW; single pregnancy); Group 2 (Six Santa Ines ewes; heat stress; nutrition at 1.5% BW; twin pregnancy); groups Group 3 (Six Morada Nova ewes; heat stress; nutrition at 0.5% of BW; single pregnancy); Group 4 (Six Morada Nova ewes; heat stress; nutrition at 1.5% BW; twin pregnancy). All the animals in the experiment were pregnant. Heat stress was induced by exposing all animals to summer heat stress in outside environment while the nutritional regimen followed was at 0.5% and 1.5% level of body weight (BW) respectively in each breed. The experiment was conducted in a completely randomized design with two breeds, two nutritional treatments and two pregnancy types, 10 repetitions for physiological parameters and six for blood parameters, with repeated measures over time. Physiological parameters (respiratory rate, pulse rate and rectal temperature) were measured with the animals at rest in the morning and afternoon, 0600-0700 and 1300-1400h, respectively, every seven days. Blood samples were collected every 14d for determination of serum glucose, triglycerides, cholesterol, urea and creatinine. We found interaction effect between breed and pregnancy type on respiratory rate and rectal temperature with greater values in Santa Inês ewes than Morada Nova ewes. However, there was no significant fixed effect of pregnancy type and supplementation level on physiological responses of breeds. Environmental factor (period of the day) had influenced the physiological responses of ewes during all gestational period. Santa Inês ewes had greater serum glucose concentration at 105d and 120d of gestation compared to the Morada Nova ewes. Morada Nova ewes had greater concentrations of triglycerides, urea at 120d, 150d and also greater cholesterol at 105d, 135d and 150d of gestation compared with Santa Inês ewes. The present result indicates that thermal condition was most important factor that modified the physiological responses of ewes in a semi-arid tropical environment.

Requirements of protein for maintenance and growth in ram hair lambs.

The experiment was conducted to determine the protein requirements for maintenance (PM) and weight gain (NPg) in Brazilian Somali lambs (n = 48). A database of information on non-castrated rams (n = 48) with an initial body weight (BW) of 13.47 ± 1.76 kg was obtained, and the comparative slaughter technique was used to assess protein requirements. Eight lambs were initially slaughtered and used as a reference for estimation of the empty body weight (EBW) and initial body composition of the remaining animals. The lambs (n = 40) were distributed in a completely randomized block design involving five experimental diets, containing 4.93, 8.65, 9.41, 10.12, and 11.24 MJ/kg dry matter (DM). Daily nitrogen retention (DNR) was calculated as the difference between the final N content in the body minus the initial content estimated from the reference animals, divided by the number of experimental days. PM was estimated from the retained N regression equation as a function of ingested N (g per kg(0.75) EBW/day), extrapolating the equation to the zero level of N intake, followed by multiplication by a factor of 6.25. The protein content of the EBW was reduced from 119.72 to 102.07 g/kg of empty body weight gain (EBG) when BW increased from 13 to 28.70 kg, respectively. The requirement of protein for maintenance was 0.80 g per kg(0.75) EBW/day. Based on information described in the present study, we recommend the value of PM 0.80 g per kg(0.75) EBW/day, for hair sheep Brazilian Somalis raised in tropical regions. We also suggest that hair sheep have different requirements for protein maintenance and gain.

Ingestive behavior and physiological parameters of goats fed diets containing peanut cake from biodiesel.

The experiment was conducted to evaluate the ingestive behavior and physiological parameters of confined goats fed peanut cake instead of soybean meal in their feed. We used 40 goats that were ¾ Boer, uncastrated, and 5 months of age on average, with an average initial weight of 15.6 ± 2.7 kg. The treatments consisted of diets with different levels of peanut cake replacing soybean meal in the concentrate (0.0, 33.33, 66.67, and 100%). The experimental design was completely randomized, with four treatments and ten repetitions. For the evaluation of feeding behavior, single animals were observed every 5 min for 24 h on 3 days. The physiological responses (respiratory rate; heart rate; rectal temperature, obtained with a rectal thermometer; and surface temperature) of the animals were evaluated at 09:00 and 15:00 h. The replacement of soybean meal with peanut cake did not change (P > 0.05) feeding behavior. The physiological parameters of the animals (P < 0.05) were altered; however, the changes appeared to be unrelated to the diet and to be due to the weather conditions. Peanut cake can replace soybean meal at 100% without causing negative effects on the feeding behavior or physiological parameters of confined ¾ Boer goats.

Intake and digestibility, rumen fermentation, and concentrations of metabolites in steers fed with peanut cake.

This study evaluated peanut cake as a replacement for soybean meal in the diet of steers. We used five castrated Holstein × Zebu steers, fistulated, with average live weight 550 kg, in a 5 × 5 Latin square design. The diets were composed of Tifton hay and ground corn with peanut cake (0, 25, 50, 75, and 100 %) as a replacement for soybean meal in the concentrate. Intake (kg/day) of dry matter, neutral detergent fiber, and crude protein were not affected by peanut cake, but ether extract intake increased linearly with the level of peanut cake in the diet. The digestibility of dry matter showed a quadratic effect, with a calculated maximum regression level of 56 % at 37 % replacement. There was no effect of the level of peanut cake on metabolizable energy intake and net energy, ammonia nitrogen, rumen pH, plasma urea, and glucose concentrations. We recommend replacing soybean meal in the concentrate with up to 40 % peanut cake.

Performance, nitrogen balance and microbial efficiency of beef cattle under concentrate supplementation strategies in intensive management of a tropical pasture.

The objective of this study was to evaluate the effect of concentrate supplementation strategies on the nutritional characteristics of beef cattle in intensive management of tropical pasture. Twenty-four Nellore steer at 250 kg body weight (BW) were used, divided into two plots, with 12 animals in each plot. The experimental area consisted of 32 paddocks with 0.25 ha of Panicum maximum cv. Mombaça. The experiment consisted of 96-day experimental periods, with three periods of 32 days. The strategies studied were P = exclusively on pasture and without concentrate supplementation (control), ES = pasture and supplemented with a concentrate low in protein, PS = pasture and supplemented with high protein content, and PES = pasture and supplementation with balanced protein-energy. There was reduced intake of DM in animals of the treatment P in relation to supplemented pasture, regardless of supplementation. Animals fed on ES showed an intake of more nutrients than the animals on PS. The CP and TDN were also lower in P than in pastures where animals received the additional types of concentrate, and the PS animals showed greater digestibility of CP and TDN than the ES animals. However, the animals exhibited similar weight gains. Animals on P ingested smaller amounts of N and had lower fecal excretion compared to the supplemented animals, but there was no difference between treatments in nitrogen balance. Urea nitrogen and urea from the blood were higher in the supplemented animals than in animals fed on pasture; these levels were also higher in PS animals compared to animals receiving ES. Both the purines absorbed and microbial protein production were similar between treatments. However, the animals fed with concentrate supplementation, independent of the strategy involved, showed higher microbial efficiency compared to animals fed exclusively on pasture.