RESUMO
In this research we investigate neuroblastoma cells cultivated on single-walled carbon nanotubes networks made by CVD method on silicon substrates. The complex analysis of grown cells made by atomic force, electron microscopy and Raman spectroscopy was carried out and the effect of nanotube growth process on proliferation factor was investigated. It is shown that despite of a weak decrease in proliferation, cell morphology remains unchanged and no physical or chemical interaction between carbon nanotubes and cells is observed. The results of the research can be used to investigate the interaction between conductive nanomaterials and cells for the development of neural replacement implants. Also they can be useful in bio-electronic interface investigation of signal propagation in neurons.
Assuntos
Proliferação de Células , Nanotubos de Carbono/química , Neuroblastoma/metabolismo , Neurônios/metabolismo , Linhagem Celular Tumoral , Humanos , Microscopia de Força Atômica , Microscopia Eletrônica de Varredura , Neuroblastoma/ultraestrutura , Neurônios/ultraestrutura , Análise Espectral RamanRESUMO
The effects of single-walled and multiwalled carbon nanotubes on proliferative activity and viability of human embryo fibroblasts and glioblastoma cells were studied. Low cytotoxic activity of single-walled carbon tubes was demonstrated. Possible mechanisms of nanotube effects on cell growth are discussed.
Assuntos
Fibroblastos/citologia , Glioblastoma/patologia , Teste de Materiais , Nanotubos de Carbono , Proliferação de Células , Sobrevivência Celular , Células Cultivadas , Embrião de Mamíferos , HumanosRESUMO
It was demonstrated that the three studied samples of carbon nanotubes of domestic production fixed on the substrate surface did not have toxic effect and could be used for cell cultivation. A biocompatible conductive coating based on carbon nanotubes and bovine serum albumin was developed. The efficacy of the coating for growing in vitro cell cultures was tested. A device was developed for electric stimulation of the cells. Local electric potential was applied to the cells using nanoscale electrodes. The results of human embryonic fibroblast cultivation in a pulsed electric field on conductive nanocomposite substrates were presented. An 26% increase in the proliferative activity of cells was observed at potentials up to 100 mV.
Assuntos
Estimulação Elétrica , Nanotubos de Carbono , Soroalbumina Bovina/química , Animais , Materiais Biocompatíveis/química , Materiais Biocompatíveis/toxicidade , Bovinos , Linhagem Celular/efeitos dos fármacos , Linhagem Celular/efeitos da radiação , Proliferação de Células/efeitos dos fármacos , Proliferação de Células/efeitos da radiação , Eletricidade , Fibroblastos/citologia , Fibroblastos/efeitos dos fármacos , Humanos , Nanotubos de Carbono/química , Nanotubos de Carbono/toxicidadeRESUMO
The structure of biocompatible nanocomposites formed by the action of laser radiation on an aqueous dispersion of albumin with carbon nanotubes has been studied by the high-resolution methods of atomic force and transmitting electron microscopy. It has been shown that the nanocomposites have a bulky structure consisting of conglomerates of nanotubes uniformly distributed in the albumin matrix. The results of the study may be useful in the production of filling nanomaterials for implants of biological tissues and organs and the control of their quality.