Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre.

The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3% sensitivity, 93.3% specificity, a 99.2% positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.

[Canine visceral leishmaniasis in Maricá, State of Rio de Janeiro: first report of an autochthonous case]. / Leishmaniose visceral canina em Maricá, Estado do Rio de Janeiro: relato do primeiro caso autóctone.

Visceral leishmaniasis is a zoonosis of public health importance, and dogs represent one of the main problems. This paper describes the first autochthonous case of canine visceral leishmaniasis in the municipality of Maricá. It provides new facts regarding the geographical distribution of Leishmania (Leishmania) chagasi in the State of Rio de Janeiro.

Evaluation of polymerase chain reaction in the routine diagnosis for tegumentary leishmaniasis in a referral centre

The present study investigated the diagnostic value of polymerase chain reaction (PCR) performed in parallel to conventional methods at an American tegumentary leishmaniasis (ATL) referral centre for diagnosis. Accuracy parameters for PCR were calculated using 130 patients with confirmed ATL (ATL group), 15 patients established with other diseases and 23 patients with a lesion suggestive of ATL, but without parasitological confirmation (NDEF group). PCR showed 92.3 percent sensitivity, 93.3 percent specificity, a 99.2 percent positive predictive value and a 13.84 positive likelihood ratio. In the NDEF group, PCR confirmed ATL in 13 of the 23 patients, seven of whom responded to leishmaniasis treatment and six who presented spontaneous healing of the lesion. PCR should be included in the routine diagnostic procedures for ATL, especially for cases found to be negative by conventional methods.

Leishmaniose visceral canina em Maricá, Estado do Rio de Janeiro: relato do primeiro caso autóctone / Canine visceral leishmaniasis in Maricá, State of Rio de Janeiro: first report of an autochthonous case

Leishmaniose visceral é uma zoonose de importância em Saúde Pública, onde os cães representam um dos maiores problemas. Este trabalho visa relatar o primeiro caso autóctone da leishmaniose visceral canina no município de Maricá, fornecendo elementos relacionados à distribuição geográfica de Leishmania (Leishmania) chagasi no Estado do Rio de Janeiro.

Visceral leishmaniasis is a zoonosis of public health importance, and dogs represent one of the main problems. This paper describes the first autochthonous case of canine visceral leishmaniasis in the municipality of Maricá. It provides new facts regarding the geographical distribution of Leishmania (Leishmania) chagasi in the State of Rio de Janeiro.

Canine visceral leishmaniasis in Marica, State of Rio de Janeiro: first report of an autochthonous case / Canine visceral leishmaniasis in Maricá, State of Rio de Janeiro: first report of an autochthonous case

Leishmaniose visceral é uma zoonose de importância em Saúde Pública, onde os cães representam um dos maiores problemas. Este trabalho visa relatar o primeiro caso autóctone da leishmaniose visceral canina no município de Maricá, fornecendo elementos relacionados à distribuição geográfica de Leishmania (Leishmania) chagasi no Estado do Rio de Janeiro.

Leishmaniose visceral canina em Maricá, Estado do Rio de Janeiro: relato do primeiro caso autóctone / Canine visceral leishmaniasis in Maricá, State of Rio de Janeiro: first report of an autochthonous case

Leishmaniose visceral é uma zoonose de importância em Saúde Pública, onde os cães representam um dos maiores problemas. Este trabalho visa relatar o primeiro caso autóctone da leishmaniose visceral canina no município de Maricá, fornecendo elementos relacionados à distribuição geográfica de Leishmania (Leishmania) chagasi no Estado do Rio de Janeiro.(AU)