RESUMO
An original diffraction model for the analysis of grazing-incidence small-angle X-ray scattering (GISAXS) from the nanoparticle Langmuir films was developed. This model relies on the concept of the 2D hexagonal paracrystal and employs the distorted-wave Born approximation that is relevant for GISAXS measurements at the air/water interface when the angle of incidence is close to the critical value. The model comprises the cases of the close-packed nanoparticle monolayer and bilayer with the AB-type layer stacking. In this way, both the lateral (along the interface) and vertical (normal to the interface) correlations of the nanoparticle positions can be analyzed. The model was applied to an in situ GISAXS study of the formation of a silver nanoparticle Langmuir film during compression at the air/water interface in the Langmuir-Blodgett trough. Spherical nanoparticles of 5.8 ± 0.6 nm diameter were employed. Different compression stages starting from the submonolayer up to the monolayer collapse via bilayer formation were analyzed in terms of the mean lateral interparticle distance, degree of paracrystal disorder, interlayer distance, vertical disorder, and layer-stacking type in the bilayer as well as the ratio between the monolayer and bilayer coverage in the final film. The model developed is applicable to any nanoparticle Langmuir film formed at the air/liquid interface to extract structural parameters on the nanoscale. The particular results obtained have direct implications on the preparation of silver plasmonic templates with "hot spots" for surface-enhanced Raman scattering.
RESUMO
We report on grazing-incidence small-angle x-ray scattering (GISAXS) study of 3D nanoparticle arrays prepared by two different methods from colloidal solutions-layer-by-layer Langmuir-Schaefer deposition and spontaneous self-assembling during the solvent evaporation. GISAXS results are evaluated within the distorted wave Born approximation (DWBA) considering the multiple scattering effects and employing a simplified multilayer model to reduce the computing time. In the model, particular layers are represented by nanoparticle chains where the positions of individual nanoparticles are generated following a model of cumulative disorder. The nanoparticle size dispersion is considered as well. Three model cases are distinguished-no shift between the neighboring chains (AA stacking), a shift equal to half of the mean interparticle distance (AB stacking) and random shift between the chains. The first two cases correspond to vertically correlated nanoparticle positions across different chains. A comparison of the experimental GISAXS patterns with the model cases enabled us to distinguish important differences between the 3D arrays prepared by the two methods. In particular, laterally ordered layers without vertical correlation of the nanoparticle positions were found in the nanoparticle multilayers prepared by the Langmuir-Schaefer method. On the other hand, the solvent evaporation under particular conditions produced highly ordered 3D nanoparticle assemblies where both laterally and vertically correlated nanoparticle positions were found.
RESUMO
In December 2008, three hospitalized cases of suspected psittacosis infection were notified by respiratory disease clinicians from a local hospital to the Regional Epidemiology Unit of Pays de la Loire, France. They all had attended a bird fair. A retrospective cohort study was conducted among exhibitors and organizers to identify potential risk factors in relation to this fair. Environmental and veterinary investigations were implemented to trace potential sources of infection. We identified two confirmed, two probable and 44 possible cases among participants. The attack rate in exhibitors and organizers was 38% (33/86). The median incubation period was 11 days (range 6-22 days). Individuals located in two particular sectors of the showroom were found to be at double the risk of developing psittacosis (relative rate 2·1, 95% confidence interval 1·03-4·18) than those in other sectors. Pooled faecal samples of birds belonging to a possible case exhibitor tested positive for Chlamydiaceae by PCR. Ventilation conditions in the showroom were inadequate. This investigation allowed the formulation of recommendations to prevent psittacosis in bird exhibitions which are held weekly in France.
Assuntos
Surtos de Doenças , Psitacose/epidemiologia , Zoonoses/epidemiologia , Animais , Aves/microbiologia , Chlamydiaceae/genética , Chlamydiaceae/isolamento & purificação , Estudos de Coortes , DNA Bacteriano/genética , Microbiologia Ambiental , Fezes/microbiologia , Feminino , França/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Estudos RetrospectivosRESUMO
An in situ small-angle x-ray scattering study of the nanoparticle displacement in a self-assembled monolayer as a function of a supporting membrane strain is presented. The average nanoparticle spacing is 6.7 nm in the unstrained state and increases in the applied force direction, following linearly the membrane strain which reaches the maximum value of 11%. The experimental results suggest a continuous mutual shift of the nanoparticles and their gradual separation with the growing stress rather than nanoparticle islands formation. No measurable shift of the nanoparticles was observed in the direction perpendicular to the applied stress.
RESUMO
INTRODUCTION: Transmissible spongiform encephalopathies (TSE) have been under epidemiological surveillance in France and in Europe since the early 1990s. The observation of iatrogenic Creutzfeldt-Jakob disease (CJD), the outbreak of bovine spongiform encephalopathy (ESB) and its probable transmission to many species gave rise to the surveillance which remains warranted by the emergence of a variant of CJD (vCJD), in 1996. STATE OF ART: In France, epidemiological surveillance is coordinated by the InVS which receives input from cases notifications addressed to INSERM Unit 708 directly by clinicians or more often following requests for 14-3-3 detection in CSF. All suspected cases are followed up until a final diagnosis is established. Thanks to the effectiveness of the French network of neuropathology, autopsies are performed in more than half of patients who die with a diagnosis of suspected CJD. Diagnostic criteria allow comparison of the incidence of the different forms of the disease in all countries with a system of surveillance. Sporadic CJD is the most frequent form of the disease with more than 80% of the cases. Its origin remains unknown. To date, cases of iatrogenic CJD referred to the French surveillance network have been caused by dura mater grafts or human growth hormone treatments administrated in the 1980s. Ten percent of TSE are of genetic origin with an autosomic dominant transmission of a mutation or an insertion located on the PRNP gene. The most recent form of the disease is vCJD which is a new form, first described in the United Kingdom in 1994. PROSPECT AND CONCLUSION: Active epidemiological surveillance remains a timely issue, particularly in France, because of the development of new cases of iatrogenic CJD after human growth hormone treatment. It is of importance in France and worldwide because of the emergence of post-transfusional cases of vCJD and the possible appearance of vCJD in persons with valine-valine or methionine-valine genotypes at codon 129.
Assuntos
Síndrome de Creutzfeldt-Jakob/epidemiologia , Adulto , Fatores Etários , Idoso , Idoso de 80 Anos ou mais , Animais , Bovinos , Síndrome de Creutzfeldt-Jakob/classificação , Síndrome de Creutzfeldt-Jakob/genética , Síndrome de Creutzfeldt-Jakob/mortalidade , Feminino , França/epidemiologia , Geografia , Humanos , Doença Iatrogênica/epidemiologia , Masculino , Pessoa de Meia-Idade , Doenças Priônicas/epidemiologiaRESUMO
The spontaneous self-assembly process of superparamagnetic nanoparticles in a fast-drying colloidal drop is observed in real time. The grazing-incidence small-angle X-ray scattering (GISAXS) technique is employed for an in situ tracking of the reciprocal space, with a 3 ms delay time between subsequent frames delivered by a new generation of X-ray cameras. A focused synchrotron beam and sophisticated sample oscillations make it possible to relate the dynamic reciprocal to direct space features and to localize the self-assembly. In particular, no nanoparticle ordering is found inside the evaporating drop and near-surface region down to a drop thickness of 90 microm. Scanning through the shrinking drop-contact line indicates the start of self-assembly near the drop three-phase interface, in accord with theoretical predictions. The results obtained have direct implications for establishing the self-assembly process as a routine technological step in the preparation of new nanostructures.
Assuntos
Nanopartículas/química , Microscopia Eletrônica de Varredura , Nanopartículas/ultraestrutura , Difração de Raios XRESUMO
OBJECTIVE: The aim of this article was to assess the seroprevalence of Lyme Borreliosis and tick-borne encephalitis (TBE) among occupationally exposed forest workers. METHODS: Workers exposed to tick bites in Eastern France were interviewed by occupational health physicians of the mutualité sociale agricole (MSA) on their sociodemographic features, their occupational activity, their last tick bite, their clinical history, and their means of prevention. Blood sampling was carried out for antibody detection. RESULTS: Among the 2975 subjects included in the study, the observed seroprevalence was 14.1% for Lyme borreliosis and 3.4% for TBE. Age, occupational activity, and place of residence significantly influenced the serological status of Lyme borreliosis. The seroprevalence was significantly higher among woodcutters (17.5%) than among other occupational categories (p<.001). Seroprevalence in Alsace (26.9%) and Lorraine (16.5%) were significantly higher than in other regions (p<0.001 and p<0.01, respectively). The seroprevalence of TBE was significantly higher in Alsace (5.5%; p<0.001). The rates of seroprevalence for both infections varied according to forest areas. The multifactorial analysis of prevention practices revealed three types of behaviors as far as protection was concerned: "rigorous", "partial", or "insufficient". CONCLUSION: These results do not change the present French indications for use of TBE vaccine. They highlight the importance of information on these diseases and the need for further studies on microbial ecology and risk-factors identification.
Assuntos
Encefalite Transmitida por Carrapatos/epidemiologia , Agricultura Florestal , Doença de Lyme/epidemiologia , Doenças Profissionais/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Anticorpos Antibacterianos/sangue , Anticorpos Antivirais/sangue , Vetores Aracnídeos/microbiologia , Vetores Aracnídeos/virologia , Mordeduras e Picadas/epidemiologia , Mordeduras e Picadas/microbiologia , Mordeduras e Picadas/virologia , Borrelia burgdorferi/imunologia , Vírus da Encefalite Transmitidos por Carrapatos/imunologia , Encefalite Transmitida por Carrapatos/prevenção & controle , Encefalite Transmitida por Carrapatos/transmissão , Feminino , França/epidemiologia , Humanos , Doença de Lyme/prevenção & controle , Doença de Lyme/transmissão , Masculino , Pessoa de Meia-Idade , Doenças Profissionais/prevenção & controle , Estudos Soroepidemiológicos , Carrapatos/microbiologia , Carrapatos/virologia , Adulto JovemRESUMO
Fifteen tularaemia cases were identified after a holiday spent at a converted mill in the Vendee region in France, between 9 and 12 August 2004. The mill was visited, and descriptive, retrospective cohort and environmental investigations were conducted. The 39 people who had stayed at the mill between 24 July and 11 August were asked about symptoms, exposure to food and animals, and leisure activities. A case was defined as a person with evidence of fever and a positive serology (seroconversion or significant rise in antibody titre, or a single titre) = 40. Culture for Francisella tularensis and polymerase chain reaction (PCR) diagnosis was carried out for drinking water, firewood, and domestic animals at the mill. Fifteen cases of tularaemia (38%) were confirmed. Twelve of the cases (80%) had the pulmonary form. None of the patients was admitted to hospital. There was a strong association between infection and participation in a dinner at the mill on 4 August (p<10-8). One of the three dogs present in the dining room was serologically positive for F. tularensis. Results of analysis of environmental samples were negative. These investigations confirmed the occurrence of a cluster of 15 tularaemia cases, in patients who were infected on the evening of 4 August, in a mill in Vend¨¦e, an endemic area for tularaemia. The investigations highlight the existence of nonspecific and benign pulmonary forms of the illness in France. The pulmonary form of infection in the human cases and the positive serology of the dog suggest contamination by inhalation of contaminated particles from the dog¡¯s fur disseminated by the dog shaking itself.
RESUMO
Fifteen tularaemia cases were identified after a holiday spent at a converted mill in the Vendee region in France, between 9 and 12 August 2004. The mill was visited, and descriptive, retrospective cohort and environmental investigations were conducted. The 39 people who had stayed at the mill between 24 July and 11 August were asked about symptoms, exposure to food and animals, and leisure activities. A case was defined as a person with evidence of fever and a positive serology (seroconversion or significant rise in antibody titre, or a single titre) = 40. Culture for Francisella tularensis and polymerase chain reaction (PCR) diagnosis was carried out for drinking water, firewood, and domestic animals at the mill. Fifteen cases of tularaemia (38%) were confirmed. Twelve of the cases (80%) had the pulmonary form. None of the patients was admitted to hospital. There was a strong association between infection and participation in a dinner at the mill on 4 August (p < 10(-8)). One of the three dogs present in the dining room was serologically positive for F. tularensis. Results of analysis of environmental samples were negative. These investigations confirmed the occurrence of a cluster of 15 tularaemia cases, in patients who were infected on the evening of 4 August, in a mill in VendAe, an endemic area for tularaemia. The investigations highlight the existence of nonspecific and benign pulmonary forms of the illness in France. The pulmonary form of infection in the human cases and the positive serology of the dog suggest contamination by inhalation of contaminated particles from the dogos fur disseminated by the dog shaking itself.
Assuntos
Surtos de Doenças , Tularemia/epidemiologia , Adolescente , Adulto , Animais , Criança , Estudos de Coortes , Cães/microbiologia , Feminino , França/epidemiologia , Francisella tularensis/isolamento & purificação , Humanos , Masculino , Pessoa de Meia-Idade , Infecções Respiratórias/epidemiologia , Tularemia/complicações , Tularemia/transmissão , Zoonoses/epidemiologiaRESUMO
In August 2004, a case of rabies was diagnosed in a puppy that had been illegally imported from Morocco to Bordeaux (France). Because a great number of people and animals were thought to have come into contact with the puppy, extensive tracing measures were implemented, and an international alert was launched to trace and treat the contacts at risk. One hundred and eighty seven people received post-exposure treatment, eight of whom also received serovaccination, and 57 animals known to have been exposed to the puppy were tested. Six months after the death of the rabid animal, none of the people treated showed any signs of rabies, nor was any secondary animal case reported. The management of this crisis highlights the importance of the role of a rapid alert system at European level. Strict application of sanitary control regulations is essential for animals introduced into EU countries, and all necessary information must be made available to EU residents travelling to rabies enzootic areas.
RESUMO
In August 2004, a case of rabies was diagnosed in a puppy that had been illegally imported from Morocco to Bordeaux (France). Because a great number of people and animals were thought to have come into contact with the puppy, extensive tracing measures were implemented, and an international alert was launched to trace and treat the contacts at risk. One hundred and eighty seven people received post-exposure treatment, eight of whom also received serovaccination, and 57 animals known to have been exposed to the puppy were tested. Six months after the death of the rabid animal, none of the people treated showed any signs of rabies, nor was any secondary animal case reported. The management of this crisis highlights the importance of the role of a rapid alert system at European level. Strict application of sanitary control regulations is essential for animals introduced into EU countries, and all necessary information must be made available to EU residents travelling to rabies enzootic areas.
Assuntos
Comércio/legislação & jurisprudência , Controle de Doenças Transmissíveis , Busca de Comunicante , Doenças do Cão/transmissão , Raiva/veterinária , Zoonoses , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Animais , Criança , Pré-Escolar , Cães , Feminino , França , Humanos , Imunoglobulinas Intravenosas/uso terapêutico , Lactente , Internacionalidade , Masculino , Pessoa de Meia-Idade , Raiva/prevenção & controle , VacinaçãoRESUMO
BACKGROUND: Hantaviral infections causing hemorrhagic fever with renal syndrome are endemic in North Eastern France. Humans are contaminated by the inhalation of aerosols contaminated by rodent faeces. In February 2003, the National reference centre (NRC) for hemorrhagic fevers detected an increased number of cases. An investigation was carried out to confirm the outbreak and take appropriate control measures. METHODS: Cases were collected by the NRC. A case was defined as a person living in France with symptoms compatible with hantaviral infection and a positive blood test both for specific Puumala IgM, and IgG. Clinical information and at-risk exposures during the 2 weeks before onset were recorded. RESULTS: In 2003, 128 cases were diagnosed (61 in 2002). The median age of patients was 38, 77% were men and 82% were hospitalized. Patients were living in North-Eastern France. Clusters were detected in the Ardennes and Oise districts. Occupation (35%) (in agriculture, forestry, and construction work), manipulation of firewood (35%), gardening (29%), and outdoors leisure (14%) were identified as at-risk exposures in these cases. CONCLUSIONS: An increased number of positive diagnoses of hantaviral infections was confirmed. The location and at-risk exposures of the cases were identical in previous investigations. Exclusion and prevention of rodents' access to houses and avoiding the inhalation of contaminated dust are the only possible prevention measures of hantaviral infections. Information about the disease and its prevention needs to be made widely available to both healthcare professionals and the general population living in endemic areas.
Assuntos
Infecções por Hantavirus/epidemiologia , Adolescente , Adulto , Idoso , Criança , Feminino , França/epidemiologia , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
The use of an inorganic phase in water-in-oil microemulsions has received considerable attention for preparing metal particles. This is a new technique, which allows preparation of ultrafine metal particles within the size range 5 nm
RESUMO
Conventional dispersion polymerization and copolymerization of low-molecular weight (conventional) unsaturated monomers allows preparation of monodisperse and micronsize polymer particles. A similar behavior can be found in the surfactant-free dispersion polymerization of non-traditional vinyl monomers, unsaturated macromonomers. The latter systems allow preparation of random, comb-like, star-like and graft copolymers as well. An interesting alternative arises with the use of amphiphilic reactive macromonomers that contain a polymerizable group and aggregate into an organized structure -- a micelle. Under such conditions the high rate of polymerization and ultrafine (microparticles) polymer dispersions are generated. Thus, the surface-active macromonomers promote the formation of micelles and polymer growth within the main reaction loci -- polymer particles. Furthermore, the surface-active compounds can be formed during the copolymerization of hydrophilic macromonomer and hydrophobic low-molecular weight comonomer. The reactive surface-active oligomeric radicals are incorporated into the polymer matrix or the particle surface layer, which prevents them from subsequent migration. Besides, the covalently bound surface-active groups at the particle surface strongly increase the colloidal stability of final polymer dispersion. This article presents a review of the current literature in the field of the surfactant-free dispersion polymerization of the polyoxyethylene unsaturated macromonomers. Besides a short introduction into some kinetic aspects of radical polymerization of traditional monomers in homogeneous and disperse systems, we mainly focus on the organized aggregation of amphiphilic polyoxyethylene macromonomers, the characterization of amphiphilic graft copolymers and their aggregation properties, and radical copolymerization of polyoxyethylene macromonomers. We discuss the birth and growth of chains, the transfer of reaction loci from the continuous phase to polymer particles, the diffusion-controlled termination, association of amphiphilic reaction by-products, the particle growth by agglomeration, the particle nucleation, the deactivation of polymer chain growth and the colloidal stability. Effects of initiator type and concentration, the surface activity of macromonomer, the macromonomer type and concentration, temperature, additives and the type of continuous phase on the kinetics of polymerization, and colloidal parameters of the reaction system are also evaluated. Variation of the polymer coil density, the polymer-polymer interaction, and polymer-solvent interaction with the molecular weight, diluent and method (light scattering, the size exclusion, etc.) are discussed. Polymerization of macromonomers provides regularly branched polymers with varied branching density. Since both the degree of polymerization and the length of branches may be varied, polymeric materials with specific properties can be prepared.
Assuntos
Polietilenoglicóis/química , Tensoativos/química , Fenômenos Químicos , Físico-Química , Coloides , Difusão , Emulsões , Radicais Livres , Cinética , Micelas , Estrutura Molecular , Peso Molecular , Tamanho da Partícula , Polímeros , Temperatura , Terminologia como Assunto , ViscosidadeRESUMO
Polymerization in micellar systems is a technique which allows the preparation of ultrafine as well as coarse latex particles. This article presents a review of the current literature in the field of radical polymerization of classical monomers in micellar systems initiated by oil-soluble initiators. Besides a short introduction to some of the kinetic aspects of emulsion polymerization initiated by water-soluble initiators, we mainly focus on the kinetics and the mechanism of radical polymerization in o/w and w/o micellar systems initiated by classical oil-soluble initiators. The initiation of emulsion polymerization of an unsaturated monomer (styrene, butyl acrylate,...) by a water-soluble initiator (ammonium peroxodisulfate) is well understood. It starts in the aqueous phase and the initiating radicals enter the monomer-swollen micelle. The formed oligomeric radicals are surface active and increase the colloidal stability of the disperse system. Besides, the charged initiating radicals might experience the energetic barrier when entering the charged particle surface. The locus of initiation with oil-soluble initiators is more complex. It can partition between the aqueous-phase and the oil-phase. Besides, the surface-active oil-soluble initiator can penetrate into the interfacial layer. The dissolved oil-soluble initiator in the monomer droplet can experience the cage effect. The small fraction of the oil-soluble initiator dissolved in the aqueous phase takes part in the formation of radicals. The oligomeric radicals formed are uncharged and therefore, they do not experience the energetic barrier when entering the polymer particles. We summarize and discuss the experimental data of radical polymerization of monomers initiated by oil-soluble initiators in terms of partitioning an initiator among the different domains of the multiphase system. The inhibitor approach is used to model the formation of radicals and their history during the polymerization. The nature of the interfacial layer and the type of oil-soluble initiator including the surface active ones are related to the kinetic and colloidal parameters. The emulsifier type and reaction conditions in the polymerization are summarized and discussed.
Assuntos
Micelas , Óleos/química , Polímeros , Emulsões , Solubilidade , ÁguaRESUMO
The principal subject discussed in the current paper is the radical polymerization of styrene in the three- and four component microemulsions stabilized by a cationic emulsifier. Polymerization in the o/w microemulsion is a new polymerization technique which allows to prepare the polymer latexes with the very high particle interface area and narrow particle size distribution. Polymers formed are very large with a very broad molecular weight distribution. In emulsion and microemulsion polymerizations, the reaction takes place in a large number of isolated loci dispersed in the continuous aqueous phase. However, in spite of the similarities between emulsion and microemulsion polymerization, there are large differences caused by the much larger amount of emulsifier in the latter process. In the emulsion polymerization there are three rate intervals. In the microemulsion polymerization only two reaction rate intervals are commonly detected: first, the polymerization rate increases rapidly with the reaction time and then decreases steadily. Essential features of microemulsion polymerization are as follows: (1) polymerization proceeds under non-stationary state conditions; (2) size and particle concentration increases throughout the course of polymerization; (3) chain-transfer to monomer/exit of transferred monomeric radical/radical re-entry events are operative; and (4) molecular weight is independent of conversion and distribution of resulting polymer is very broad. The number of microdroplets or monomer-starved micelles at higher conversion is high and they persist throughout the reaction. The high emulsifier/water ratio ensures that the emulsifier is undissociated and can penetrate into the microdroplets. The presence of a large amount of emulsifier strongly influences the reaction kinetics and the particle nucleation. The mixed mode particle nucleation is assumed to govern the polymerization process. At low emulsifier concentration the micellar nucleation is dominant while at a high emulsifier concentration the interaction-like homogeneous nucleation is operative. Furthermore, the paper is focused on the initiation and nucleation mechanisms, location of initiation locus, and growth and deactivation of latex particles. Furthermore, the relationship between kinetic and molecular weight parameters of the microemulsion polymerization process and colloidal (water/particle interface) parameters is discussed. In particular, we follow the effect of initiator and emulsifier type and concentration on the polymerization process. Besides, the effects of monomer concentration and additives are also evaluated.
Assuntos
Estireno/química , Cátions , Coloides/química , Emulsões , Cinética , Polímeros , Fatores de Tempo , ÁguaRESUMO
In France, the number of reports of suspected CJD increased from 1998 to 2000, probably due to the increase in the requests for biological tests: respectively 459 in 1998, 590 in 1999, and 823 in 2000. For all three years, the distribution by sex is similar, with a sex ratio (M/F) of 1.05. The proportion of suspected cases aged under 50 remained stable (16% of all reports in 1998-2000). The number of sporadic CJD, confirmed or probable, was stable, with a mortality ratio of 1.38 per one million in 1998, 1.56 in 1999, and 1.41 in 2000.
Assuntos
Síndrome de Creutzfeldt-Jakob/epidemiologia , Distribuição por Idade , Códon , Síndrome de Creutzfeldt-Jakob/genética , Síndrome de Creutzfeldt-Jakob/patologia , França/epidemiologia , Humanos , Mutação , Vigilância da População , Fatores de RiscoRESUMO
Five clustered cases of leptospirosis were diagnosed in the area of Rochefort, France, in June 2001, among teenagers who had swum in the Genouillé canal. The symptoms included fever, headache, abdominal pain and vomiting, chills and myalgia. Three cases were confirmed by PCR and serology. The mean cumulative duration of bathing was significantly higher in cases (23.8 hours) compared to controls (14.4 hours). No other particular risk factor was observed. The environmental investigation revealed the presence of rodents excreting of leptospires near the bathing area. For all antigens considered, the occurence of seropositive rodents was 30.8%, L. icterohaemorrhagiae being the predominant serogroup (23,1%).
Assuntos
Surtos de Doenças/prevenção & controle , Leptospirose/epidemiologia , Leptospirose/transmissão , Adolescente , Adulto , Distribuição por Idade , Animais , Estudos de Casos e Controles , Criança , Vetores de Doenças , Feminino , França/epidemiologia , Água Doce/microbiologia , Humanos , Leptospira/isolamento & purificação , Leptospirose/diagnóstico , Masculino , Ratos , Fatores de Risco , Testes Sorológicos , Distribuição por Sexo , Natação , Microbiologia da ÁguaRESUMO
One of the objectives of the surveillance systems implemented by the French National Institute for Public Health Surveillance is to detect communicable diseases and to reduce their impact. For emerging infections, the detection and risk analysis pose specific challenges due to lack of documented criteria for the event. The surveillance systems detect a variety of events, or "signals" which represent a potential risk, such as a novel germ, a pathogen which may disseminate in a non-endemic area, or an abnormal number of cases for a well-known disease. These signals are first verified and analyzed, then classified as: potential public health threat, event to follow-up, or absence of threat. Through various examples, we illustrate the method and criteria which are used to analyze and classify these events considered to be emerging. The examples highlight the importance of host characteristics and exposure in groups at particular risk, such as professionals in veterinarian services, health care workers, travelers, immunodepressed patients, etc. The described method should allow us to identify future needs in terms of surveillance and to improve timeliness, quality of expertise, and feedback information regarding the public health risk posed by events which are insufficiently documented.