RESUMO
The maize GapC4 promoter harbours a complex arrangement of cis-sequences involved in activation of anaerobic gene expression in tobacco. As shown by transient expression assays, four copies of a 50 bp anaerobic response element (ARE) increase anaerobic gene expression compared to the ARE alone. Expression strength is similar to a 190 bp fragment that contains most sequences required for anaerobic expression, including the 50 bp ARE. This supports the notion that redundancy of cis-acting sequences contribute to the anaerobic expression strength of the promoter. Mutation analysis of the 50 bp ARE revealed that cis-regulatory sequences are located within 30 bp at the 5' end of the ARE. Of these 30 bp a putative binding site for a Myb transcription factor is essential for anaerobic induction. The TATA box of the GapC4 promoter is also required for anaerobic gene expression and is bound specifically by a recombinant TATA box binding protein (TBP) from tobacco. A model for anaerobic induction of the GapC4 minimal promoter in tobacco that summarizes the presented data is discussed.
Assuntos
Regulação da Expressão Gênica de Plantas , Genes de Plantas , Genes myb , TATA Box , Anaerobiose , Sítios de Ligação , Gliceraldeído-3-Fosfato Desidrogenases/genética , Nicotiana/genética , Zea mays/genéticaRESUMO
Non-phosphorylating glyceraldehyde 3-phosphate dehydrogenase (GAPDH, NADP-specific, EC 1.2.1.9) operates in the cytosol of autotrophic eukaryotes where it generates NADPH for biosynthetic processes from photosynthetic glyceraldehyde 3-phosphate exported from the chloroplast by the phosphate translocator. Here we report the first cloning and characterization of cDNAs encoding complete polypeptide chains of nonphosphorylating GAPDH from pea and maize by using oligonucleotide probes derived from amino acid sequences determined for the purified enzyme. Unexpectedly, nonphosphorylating GAPDH cannot be aligned with the well-known sequences of phosphorylating GAPDH, but shares about 30% amino acid identity with various specialized and non-specialized aldehyde dehydrogenases (ALDHs) of eubacteria and eukaryotes. A phylogenetic analysis of this ALDH superfamily reveals a complex evolutionary pattern with numerous major branches carrying genes from eubacteria, eukaryotes, or both, encoding enzymes that are specific or non-specific for particular aldehyde substrates. This topology suggests a concomitant emergence of multiple substrate specificities from non-specialized ALDH during an early evolutionary phase of intense metabolic diversification. Although unrelated at the sequence level, non-phosphorylating aldehyde dehydrogenases and phosphorylating GAPDH resemble one another with respect to catalytic hydride transfer and covalent thiol ester formation. Whether or not this reflects an ancestral relationship can only be decided when crystallographic data for ALDH enzymes have become available.
Assuntos
Gliceraldeído-3-Fosfato Desidrogenases/química , Plantas/enzimologia , Sequência de Aminoácidos , Sequência de Bases , Evolução Biológica , Catálise , Clonagem Molecular , DNA Complementar , Fabaceae/enzimologia , Dados de Sequência Molecular , Fosforilação , Plantas Medicinais , Homologia de Sequência de Aminoácidos , Zea mays/enzimologiaRESUMO
A nuclear gene encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase from maize (subunit GAPC1, gene Gpc1) and 2.2 x 10(3) base-pairs of its 5' flanking region have been cloned and sequenced. The structure of the maize Gpc1 gene (10 introns) is different from that of the maize gene encoding subunit GAPA of chloroplast glyceraldehyde-3-phosphate dehydrogenase (1 intron) and relatively similar to that of the chicken gene (11 introns). Introns in the Gpc1 gene show a positional polarity; the more 3' their position, the more they are displaced relative to introns in the chicken gene. The Gpc1 gene and other nuclear genes from maize are associated with CpG islands, the relative size of which determines the degree of codon bias in the gene. The promoter of the maize Gpc1 gene contains an anaerobic regulatory element and a pyrimidine box upstream from the TATA box and within intron 1. Southern blotting analyses and Northern hybridizations suggest that there are three functional Gpc genes in maize whose transcript levels are controlled differentially by anaerobiosis. In spite of its "typical" anaerobic promoter, the Gpc1 gene does not seem to be an anaerobic gene in vivo.
Assuntos
Anaerobiose , Genes , Gliceraldeído 3-Fosfato/genética , Gliceraldeído/análogos & derivados , Metabolismo , Zea mays , Sequência de Bases , Evolução Biológica , Citosol , Éxons , Íntrons , Modelos Genéticos , Dados de Sequência Molecular , Regiões Promotoras GenéticasRESUMO
A cDNA-library has been constructed from Nicotiana plumbaginifolia seedlings, and the non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase (GapN, EC 1.2.1.9) was isolated by plaque hybridization using the cDNA from pea as a heterologous probe. The cDNA comprises the entire GapN coding region. A putative polyadenylation signal is identified. Phylogenetic analysis based on the deduced amino acid sequences revealed that the GapN gene family represents a separate ancient branch within the aldehyde dehydrogenase superfamily. It can be shown that the GapN gene family and other distinct branches of the superfamily have its phylogenetic origin before the separation of primary life-forms. This further demonstrates that already very early in evolution, a broad diversification of the aldehyde dehydrogenases led to the formation of the superfamily.
Assuntos
Gliceraldeído-3-Fosfato Desidrogenases/genética , Nicotiana/enzimologia , Proteínas de Plantas/genética , Plantas Tóxicas , Aldeído Desidrogenase/genética , Sequência de Aminoácidos , Proteínas de Bactérias/genética , Sequência de Bases , Southern Blotting , Clonagem Molecular , Genes de Plantas , Dados de Sequência Molecular , Família Multigênica , Fosforilação , Filogenia , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Streptococcus mutans/genéticaRESUMO
Photosynthetic eukaryotes typically possess two distinct glyceraldehyde-3-phosphate dehydrogenases, an NAD+ -specific enzyme in the cytosol (GapC: EC 1.2.1.12) and an NADP+ -dependent enzyme in the chloroplast (GapAB: EC 1.2.1.13). The gymnosperm Pinus sylvestris is an exception in that it is known to express a gene encoding a transit peptide-bearing GapC-like subunit that is imported into chloroplasts (GapCp), but the enzymatic properties of this novel GAPDH have not been described from any source. We have expressed the mature GapCp unit from Pinus in Escherichia coli and have characterized the active enzyme. GapCp has a specific activity of 89 units per milligram and is strictly NAD+ -dependent, showing no detectable activity with NADP+. Values of the apparent Km for NAD+ and glyceraldehyde-3-phosphate were determined as 62 and 344 microM, respectively. The Pinus GapCpl gene possesses 12 introns, two in the region encoding the transit peptide and ten in the region encoding the mature subunit, all of which are found at positions strictly conserved across genes for higher plant GapC. A cDNA encoding a homologue of GapCp was isolated from the heterosporous fern Marsilea quadrifolia, indicating that NAD+ -dependent chloroplast GAPDH also occurs in other higher plants.
Assuntos
Cycadopsida/genética , Genes de Plantas , Gliceraldeído-3-Fosfato Desidrogenases/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Cloroplastos/enzimologia , Clonagem Molecular , Cycadopsida/enzimologia , Escherichia coli , Evolução Molecular , Gliceraldeído-3-Fosfato Desidrogenases/química , Gliceraldeído-3-Fosfato Desidrogenases/metabolismo , Humanos , Íntrons , Cinética , Dados de Sequência Molecular , NAD/metabolismo , Filogenia , Pinus sylvestris , Mapeamento por Restrição , Alinhamento de Sequência , Homologia de Sequência de AminoácidosRESUMO
With primers constructed against highly conserved regions of tRNA genes (trnTUGU, trnLUAA and trnFGAA) in chloroplast DNA, we have amplified two different non-coding spacers and one intron from four species within the genus Echium L. (Boraginaceae) and from two confamilial outgroups. The trnTUGU-trnLUAA intergenic spacer contains a greater number of polymorphic sites than the trnLUAA intron or the trnLUAA-trnFGAA intergenic spacer. We analyzed a total of 11 kb of sequence data from this non-coding DNA. Total nucleotide divergence between Echium species is on the order of 1% for these regions, all of which possess infrageneric length polymorphisms. The latter two regions contain indels which occur only in the 14 Macaronesian Island endemic species of Echium studied and suggest that these may form a monophyletic group.
Assuntos
DNA de Cloroplastos/genética , Filogenia , Plantas/genética , Primers do DNA/genética , Dados de Sequência Molecular , Reação em Cadeia da Polimerase , Polimorfismo Genético/genéticaRESUMO
A DNA fragment transforming the cells of the cyanobacterium Synechocystis sp. PCC 6803 to amitrole (3-amino-1,2,4-triazole) resistance was cloned from the Atr2 mutant resistant to this herbicide. The transforming activity of the cloned fragment was shown to be related to the missence-mutation "Val250-->Leu250" in the glmS gene encoding glucosamine-6-phosphate synthase, a key enzyme of cell wall synthesis. The amino acid substitution is localized in the central nonconservative part of the GlmS protein, far from two reaction centers positioned at the ends of a polypeptide. It is suggested that the mutant protein has lost sensitivity to amitrole. In the wild type strain, this herbicide causes conditional glucosamine auxotrophy (exogenous glucosamine restores ability of the cells to row in the presence of the lethal amitrole concentrations). Val250 is proposed to be allosteric binding site of AM in the GlmS protein of cyanobacterium.
Assuntos
Amitrol (Herbicida)/farmacologia , Cianobactérias/efeitos dos fármacos , Glutamina-Frutose-6-Fosfato Transaminase (Isomerizante)/genética , Herbicidas/farmacologia , Sequência de Aminoácidos , Cianobactérias/genética , Cianobactérias/metabolismo , Dados de Sequência Molecular , Mutação de Sentido IncorretoRESUMO
The role of the prqR gene in the regulation of the adaptive response of the cyanobacterium Synechocystis sp. PCC6803 to the oxidative stress induced with methyl viologen (MV) was studied. For this, transcription activity of prqR and the genes, which may be involved in the control of resistance to MV, was determined by means of Northern blot hybridization in wild-type cells and in the MV-resistant Prq20 mutant with a mutation located in the DNA-binding domain of the PrqR protein. It was ascertained that the prqR gene is a component of the prqR-prqA operon and down regulates its transcription. In cells of the wild-type strain containing MV, the autorepressor activity of the PrqR protein enhances and transcription of mvrA and sodB genes encoding an respectively assumed transporter protein and iron-containing superoxide dismutase increases. The prqR gene may be involved in the negative, indirect control of transcription of these genes. The Prq20 mutant is characterized by an MV-independent derepression of the prqR-prqA operon and by a slightly increased transcription of mvrA and sodB genes not stimulated by MV. Nevertheless, the expression of mvrA and sodB genes was lower than in wild-type cells after the MV treatment. On the strength of this evidence, it is assumed that the main mechanism underlying for the resistance to MV in the Prq20 mutant is derepression of the prqA gene, the product of which is homologous to multidrug transporters, drug efflux proteins.
Assuntos
Cianobactérias/genética , Regulação Bacteriana da Expressão Gênica , Genes Bacterianos , Herbicidas/farmacologia , Paraquat/farmacologia , Proteínas Repressoras/genética , Cromossomos Bacterianos , Cianobactérias/efeitos dos fármacos , Cianobactérias/metabolismo , Mutação , Óperon , Estresse Oxidativo , Estrutura Terciária de Proteína , Proteínas Repressoras/metabolismo , Transcrição GênicaRESUMO
The development of NADP- and NAD-dependent glyceraldehyde 3-phosphate dehydrogenase and NADH-specific glyoxylate reductase was followed in Sinapis alba cotyledons grown in the dark or under continuous red and far red light. All three enzyme activities are promoted by light, continuous far red light being more than twice as effective as continuous red light. The activities of the NADP-glyceraldehyde 3-phosphate dehydrogenase and glyoxylate reductase increase in the far red light from 36 to 96 hours. They remain constant until at least 120 hours after sowing and are respectively 11 and 6 times higher than the maximum dark activities. Contrary to this, the activity of the NAD-glyceraldehyde 3-phosphate dehydrogenase is scarcely more than doubled under continuous far red irradiation relative to its maximal dark level, and its time course curve is displaced along the time axis, with the activity increasing between 24 and 72 hours after sowing.The increase in activity of NADP-glyceraldehyde 3-phosphate dehydrogenase and glyoxylate reductase is inhibited by d-threo-chloramphenicol but not by the l-threo isomer at concentrations of 200 micrograms per milliliter or less, whereas the slight inhibitory effect of chloramphenicol on the NAD-glyceraldehyde 3-phosphate dehydrogenase is not stereospecific. The three enzyme activities are inhibited by cycloheximide.When Knop's solution is used as growth medium it strongly promotes NADP-glyceraldehyde 3-phosphate dehydrogenase activity in vivo and is twice as effective in the red light as in the far red light and dark. The activity of NAD-glyceraldehyde 3-phosphate dehydrogenase is only slightly and almost equally enhanced by Knop's solution in the dark, red, and far red light.These results are consistent with the following conclusions. [List: see text].
RESUMO
Aggregation of glyceraldehyde-3-P dehydrogenase (NADP) (EC 1.2.1.13) from Sinapis alba seedlings during gel filtration on Sepharose 6B is dependent on the presence of a fraction ("binding fraction") which can be separated from the enzyme by precipitation with 55% ammonium sulfate. Association of the enzyme with this binding fraction is NAD-dependent whereas NADP(+) causes release. Dithioerythritol (2 mM) has no influence on these reversible processes.Binding fractions, partially purified by ammonium sulfate and acetone fractionation, were submitted to dodecylsulfate-polyacrylamide gel electrophoresis. They always contain one or two dominant polypeptides with apparent molecular weights 42,000 and 58,000. The 42,000 polypeptide comigrates during dodecylsulfate electrophoresis with the corresponding subunit of the enzyme. It comprises up to 70% of the total protein in partially purified binding fractions and can be regarded as a major protein in seedling extracts.The differential transport behavior of glyceraldehyde-3-P dehydrogenase (NADP) on Sephadex G-200 in the presence of NAD(+) and NADP(+) can be used as a simple and effective purification procedure. The enzyme isolated in this way has an isoelectric point of about 4.5 and maintains under all tested conditions a heterogeneous subunit composition of at least three different polypeptide chains (apparent molecular weights, 39,000, 42,000, 43,000).The present data suggest that NAD(P)-controlled aggregation of glyceraldehyde-3-P dehydrogenase (NADP) from Sinapis alba L. is due primarily to enzyme association with a separate binding fraction rather than to enzyme polymerization. It is possible that a major component of this binding fraction, the 42,000 polypeptide, consists of "surplus" nonactive enzyme subunits, which self-associate and interact with the NAD-conformer of the enzyme.
RESUMO
Extracts from 13 different angiosperm species (spinach, mustard, pea, bean, tomato, cucumber, pumpkin, maize, sorghum, rye, wheat, oats, barley) were submitted to electrophoresis under nondenaturing conditions and stained for enzyme activities of cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases by a modified tetrazolium test of high sensitivity. Zymograms of the cytosolic enzyme revealed a single band of similar electrophoretic mobility for all but one species, the tomato, which displayed an ordered set of five different bands. In contrast, zymograms of the chloroplast dehydrogenase are highly different, containing between two and five distinct bands of variable electrophoretic mobilities according to the plant species examined. This variability of the native chloroplast enzyme is paralleled by a remarkable interspecific heterogeneity of the enzyme with respect to subunit size and number, as shown by dodecylsulfate electrophoresis of the purified chloroplast enzyme from 11 different angiosperm species. The present data suggest that cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases belong to two separate protein families of different evolutionary rate. While the cytosolic enzyme is probably an extremely conservative protein like the corresponding enzymes from animals, yeast and bacteria, the chloroplast enzyme seems to change rather rapidly during evolution.
Assuntos
Evolução Biológica , Cloroplastos/enzimologia , Citosol/enzimologia , Gliceraldeído-3-Fosfato Desidrogenases/genética , Plantas/enzimologiaRESUMO
1. NAD(P)+-induced changes in the aggregational state of prepurified NADP-linked glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13) were used to isolate the enzyme from Spinacia oleracea, Pisum sativaum and Hordeum vulgare. Each of the three plant species contains two separate isoenzymes. Isoenzyme 1 (fast moving during conventional electrophoresis) precipitates with the ammonium sulfate fraction 55--70% saturation. It shows two separate subunits in dodecylsulfate gels, which are probably arranged as A2B2 in the native enzyme molecule. Isoenzyme 2 (slow moving during conventional electrophoresis) precipitates with the ammonium sulfate fraction 70--95%. It contains a sigle subunit of the same Mr as subunit A in isoenzyme 1 and is apparently a tetramer (A4). The molecular weights of subunits A/B for spinach, peas and barley were determined as 38,000/40,000, 38,000/42,000 and 36,000/39,000 respectively. 2. The NAD-specific glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.12) was purified from Spinacia oleracea and Pisum sativum by affinity chromatography on blue Sepharose CL-6B. The enzyme from both plant species is shown to be a tetramer of subunits with Mr 39,000. 3. The present findings contrast with heterogeneous results obtained previously by other authors. These results suggested that there are considerable interspecific differences in the quaternary structure of glyceraldehyde-3-phosphate dehydrogenases from higher plants.
Assuntos
Gliceraldeído-3-Fosfato Desidrogenases , Plantas/enzimologia , Gliceraldeído-3-Fosfato Desidrogenases/isolamento & purificação , Substâncias Macromoleculares , NAD/metabolismo , NADP/metabolismo , Peptídeos/análise , Conformação Proteica , Especificidade da EspécieRESUMO
Two cDNA clones, encoding cytosolic and chloroplast glyceraldehyde-3-phosphate dehydrogenases (GAPDH) from mustard (Sinapis alba), have been identified and sequenced. Comparison of the deduced amino acid sequences with one another and with the GAPDH sequences from animals, yeast and bacteria demonstrates that nucleus-encoded subunit A of chloroplast GAPDH is distinct from its cytosolic counterpart and the other eukaryotic sequences and relatively similar to the GAPDHs of thermophilic bacteria. These results are compatible with the hypothesis that the nuclear gene for subunit A of chloroplast GAPDH is of prokaryotic origin. They are in puzzling contrast with a previous publication demonstrating that Escherichia coli GAPDH is relatively similar to the eukaryotic enzymes [Eur. J. Biochem. 150, 61-66 (1985)].
Assuntos
Cloroplastos/enzimologia , Citosol/enzimologia , DNA/análise , Gliceraldeído-3-Fosfato Desidrogenases/genética , Sequência de Aminoácidos , Bacillus/enzimologia , Sequência de Bases , Clonagem Molecular , Escherichia coli/enzimologia , Gliceraldeído-3-Fosfato Desidrogenases/análise , Mostardeira/enzimologia , Plantas MedicinaisRESUMO
Subunits A and B of chloroplast glyceraldehyde-3-phosphate dehydrogenase are synthesized as higher molecular weight precursors when polyadenylylated mRNA from angiosperm seedlings is translated in vitro by wheat germ ribosomes. The in vivo levels of mRNA coding for these precursors are strongly light dependent, and the increase in translational activity stimulated by continuous white light, relative to dark-grown seedlings, is at least 5- to 10-fold for the seven plant species investigated. As opposed to this, light does not seem to change mRNA levels coding for cytosolic glyceraldehyde-3-phosphate dehydrogenase, and the polypeptides synthesized in vitro have the same size as the authentic subunits. In addition, precursors of the chloroplast enzyme were identified for 12 different angiosperm species and compared with their respective subunits synthesized in vivo. The patterns of the in vitro and in vivo products correlate in several major characteristics. They both display a remarkable interspecific heterogeneity with respect to size and number of polypeptides. The peptide extensions of the enzyme precursors calculated from these data vary between 4,000 and 12,000 daltons and seem to fall into three major size classes. The present data demonstrate that chloroplast glyceraldehyde-3-phosphate dehydrogenase, like its cytosolic counterpart, is encoded in the nucleus. Yet, the two dehydrogenases are controlled differently at both the ontogenetic and phylogenetic levels. They follow separate biosynthetic pathways with respect to light regulation, post-translational processing, and transport and also exhibit different evolutionary rates. The fast evolutionary change observed for the chloroplast enzyme contrasts sharply with the conservative structure and sequence of the cytosolic enzyme.
RESUMO
Ammonium sulfate chromatography has been employed to separate glyceraldehyde 3-phosphate dehydrogenases (GPD) of Sinapis alba cotyledons of various developmental stages. Cotyledons of dark-grown seedlings possess one major NAD-specific enzyme designated NAD-GPD I. Irradiation with continuous far red light leads to a strong increase in NADP-GPD activity and to the formation of a second NAD activity designated NAD-GPD II. These two activities occur in a constant ratio during cotyledon development, and they are eluted together in ammonium sulfate chromatography. In a later stage of cotyledon development the light-dependent increase in NAD-GPD II is matched by an equivalent decrease in NAD-GPD I. These data suggest that the chloroplast marker enzyme NADP-GPD (EC 1.2.1.13) also has NAD activity and that the light-dependent formation of this bifunctional enzyme is correlated with activity changes of the NAD-GPD of cytoplasmic glycolysis (EC 1.2.1.12).
RESUMO
In a previous publication (Cerff, R. (1979) Eur. J. Biochem., 94, 243--247) we demonstrated that chloroplast NADP-linked glyceraldehyde-3-P dehydrogenase (EC 1.2.1.13) from higher plants consists of two separate isoenzymes with apparent subunit compositions A2B2 (isoenzyme 1) and A4 (isoenzyme 2), where Subunits A and B are distinguished by slightly different molecular weights (A smaller than or approximately to B). In the present study we compare isoenzymes 1 and 2 from Sinapis alba and Hordeum vulgare on the basis of antigenic cross-reactivity, tryptic peptides, and amino acid composition. Isoenzymes 1 and 2 show immunochemical identity. They also have very similar tryptic peptide maps and amino acid compositions. This strongly suggests that Subunits A and B of the NADP-linked enzyme are very similar in primary sequence. As opposed to this, cytoplasmic NAD-specific glyceraldehyde-3-P dehydrogenase (EC 1.2.1.12) does not cross-react with antisera raised against the NADP-linked enzyme. Furthermore, tryptic peptide maps of the NAD-specific enzyme show little or no similarity with those of the NADP-linked enzyme. This indicates that the subunits of the NADP-linked enzyme and the subunit of the NAD-specific enzyme are different proteins coded by separate genes. The differences in the amino acid compositions between the two species corresponds to a SdeltaQ value of 21, suggesting some sequence resemblance and a common phylogenetic origin.
Assuntos
Gliceraldeído-3-Fosfato Desidrogenases , Plantas/enzimologia , Aminoácidos/análise , Gliceraldeído-3-Fosfato Desidrogenases/isolamento & purificação , Hordeum/enzimologia , Imunodifusão , Isoenzimas/isolamento & purificação , Substâncias Macromoleculares , Peso Molecular , Fragmentos de Peptídeos/análise , Especificidade da Espécie , TripsinaRESUMO
In this study we have determined gap sequences from nine different spirochetes. Phylogenetic analyses of these sequences in the context of all other available eubacterial and a selection of eukaryotic Gap sequences demonstrated that the eubacterial glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene diversity encompasses at least five highly distinct gene families. Within these gene families, spirochetes show an extreme degree of sequence divergence that is probably the result of several lateral gene transfer events between spirochetes and other eubacterial phyla, and early gene duplications in the eubacterial ancestor. A Gap1 sequence from the syphilis spirochete Treponema pallidum has recently been shown to be closely related to GapC sequences from Euglenozoa. Here we demonstrate that several other spirochetal species are part of this cluster, supporting the conclusion that an interkingdom gene transfer from spirochetes to Euglenozoa must have occurred. Furthermore, we provide evidence that the GAPDH genes present in the protists Parabasalia may also be of spirochetal descent.
Assuntos
Variação Genética , Gliceraldeído-3-Fosfato Desidrogenases/genética , Spirochaetales/genética , Sequência de Aminoácidos , Animais , Células Eucarióticas/fisiologia , Evolução Molecular , Gliceraldeído-3-Fosfato Desidrogenases/química , Gliceraldeído-3-Fosfato Desidrogenases/classificação , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Spirochaetales/enzimologiaRESUMO
The promoter of the maize glyceraldehyde-3-phosphate dehydrogenase 4 gene (GapC4) confers strong, specific and ubiquitous anaerobic reporter gene expression in tobacco. To identify factors required for heterologous anaerobic gene expression, 19 progressive 5' and 3' promoter deletions were linked to a chimeric GapC4 TATA box-beta-glucuronidase (GUS) reporter gene construct and transformed into tobacco. In all transgenic lines aerobic expression values were in the range obtained for negative controls while histochemical GUS assays reveal some weak expression in roots only. Anaerobic induction of about 100-fold to more than 1000-fold above unspecific background is mediated by a region of about 190 bp of the GapC4 promoter. Anaerobic reporter gene induction strongly decreases upon deletion of a 20 bp fragment from -286 to -266 relative to the transcription start point. This fragment harbours putative cis-acting sequences. Electrophoretic mobility shift assays with a 50 bp fragment harbouring these cis sequences reveal a high-mobility complex that is formed with nuclear extracts from aerobic and anaerobic leaf tissue while an additional low-mobility complex is anaerobiosis-specific. The formation of the high-mobility complex requires the sequence GTGGGCCCG. The 50 bp fragment alone confers weak and orientation-dependent anaerobic induction to a GapC4 TATA box-beta-glucuronidase (GUS) reporter gene.
Assuntos
DNA de Plantas/metabolismo , Gliceraldeído-3-Fosfato Desidrogenases/genética , Proteínas Nucleares/metabolismo , Regiões Promotoras Genéticas/genética , Zea mays/enzimologia , Anaerobiose , Sequência de Bases , DNA de Plantas/genética , Regulação da Expressão Gênica de Plantas , Glucuronidase/genética , Glucuronidase/metabolismo , Dados de Sequência Molecular , Plantas Geneticamente Modificadas , Plantas Tóxicas , Ligação Proteica , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Sequências Reguladoras de Ácido Nucleico , Deleção de Sequência , Homologia de Sequência do Ácido Nucleico , Nicotiana/genética , Ativação Transcricional , Zea mays/genéticaRESUMO
We have sequenced and analysed the transaldolase (tal) genes from two cyanobacteria, Anabaena variabilis (ATCC 29413) and Synechocystis sp. PCC 6803, which are filamentous heterocyst-forming and unicellular organisms, respectively. The deduced amino acid sequences of the two cyanobacterial tal genes are 78% identical and are highly homologous to both eubacterial and eukaryotic transaldolases (Escherichia coli, two yeasts, and man) with values ranging from 54 to 60% amino acid identity. In contrast, the transaldolase homologous sequences from the cyanobacterium Nostoc sp. ATCC 29133, from Mycobacterium leprae, and the partial sequence from the higher plant Arabidopsis thaliana have a much lower degree of homology with each other and relative to the sequences mentioned above. These data indicate three different types of transaldolases.
Assuntos
Anabaena/genética , Cianobactérias/genética , Genes Bacterianos , Transaldolase/genética , Sequência de Aminoácidos , Anabaena/enzimologia , Bactérias/enzimologia , Bactérias/genética , Sequência de Bases , Cianobactérias/enzimologia , Dados de Sequência Molecular , Via de Pentose Fosfato/genética , Filogenia , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Transaldolase/classificaçãoRESUMO
The nuclear gene encoding chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from maize has been cloned and sequenced. The gene is G + C rich in its coding sequences and, in addition, contains a CpG-rich region surrounding the promoter. Further upstream several enhancer-like repetitions have been identified that may control the light- and phytochrome-mediated expression of this gene. The gene is interrupted by three introns. Introns 1 and 2 are located within the sequence encoding the transit peptide, dividing it into three parts, each containing one of the three major homology blocks typical for transit peptides of nucleus-encoded chloroplast proteins. Intron 3 is located at codon 166 (glycine) at the same nucleotide position as intron 1 in the GAPDH gene from the nematode Caenorhabditis elegans, suggesting that this intron was present in the parental GAPDH gene from which these two modern descendants originated. Intron 3 divides the GAPDH protein into its two constituent domains, the NAD-binding and the catalytic domain, immediately after helix alpha 1 at a position homologous to that of intron 9 in the gene for maize alcohol dehydrogenase, thereby confirming the prediction of Brändén et al. on the basis of gene-protein structure correlations in maize alcohol dehydrogenase for the placement of introns in the GAPDH gene [Brändén, C.I., Eklund, H., Cambillau, C. & Pryor, A.J. (1984) EMBO J. 3, 1307-1310]. These results suggest that intron 3 is an archetypical relic of early GAPDH and alcohol dehydrogenase evolution, whereas introns 1 and 2 were implicated in the evolution of chloroplast transit peptides.