Exploring the Oxidative Effects of the Microbial Electro-Fenton Process on the Depolymerization of Lignin Extracted from Rice Straw in a Bio-Electrochemical System Coupled with Wastewater Treatment.

Lignin is a potential renewable feedstock to produce value-added compounds, but the overwhelming bulk of it is either burned for energy or discarded as waste. This paper addressed two critical issues: waste-to-value generation and management by demonstrating the in situ depolymerization of lignin extracted from waste rice straw utilizing the microbial electro-Fenton process in a microbial peroxide-producing cell (MPPC), a type of bio-electrochemical cell, for value addition while synchronously treating wastewater. The MPPC electrochemical voltage yields of 0.171 ± 0.05-0.497 ± 0.2 V produced 9 ± 0.43-34 ± 0.11 mM of H2O2, which was utilized to depolymerize lignin at various concentrations. Interestingly, a direct correlation was observed between lignin depolymerization and H2O2 concentration, while Fourier-transform infrared spectroscopy data revealed a constant disruption of the lignin structure accurately in the wavenumber region of 1000-1750 cm-1 irrespective of the H2O2 concentration. Carboxylic acid derivatives, benzopyran, hexanoic acid, and other valuable compounds were detected in the LC QTOF MS data from the depolymerized lignin mixture. Remarkably, SEM analysis demonstrated morphological changes in depolymerized lignin induced by the oxidative effects of hydroxyl radicals. Biochemical oxygen demand and chemical oxygen demand removal was 60 ± 3-85 ± 1% in anodic wastewater treatment. This research provides a sustainable and efficient technique for lignin valorization and wastewater treatment.

Catalytic valorization of Kraft lignin into feedstock chemicals with methyltrioxorhenium (MTO) catalyst in microbial electrochemical cell.

In this study, the authors investigate a novel approach to valorize Kraft lignin using the catalyst Methyltrioxorhenium (MTO) in tandem with in-situ produced H2O2 in a Microbial Electrochemical Cell (MEC). This study demonstrates the in-situ oxidation of Kraft lignin using different concentrations of MTO catalyst (2 mM to 8 mM) and H2O2 (5.24 ± 0.40 mM to 8.91 ± 0.70 mM) in three MECs. The depolymerized Kraft lignin samples were characterized using FTIR, CHNS/O, and 1H NMR analysis. The MTO/H2O2 combination showed high selectivity towards the oxidation of Kraft lignin, resulting in both aromatic ring and side chain cleavage reactions and the production of valuable feedstock chemicals. The oxidation also led to a reduction of 68.42 % to 78.18 % in Chemical Oxygen Demand (COD) of lignin. The selective oxidation favored the recovery of Guaiacyl (G) unit-derived feedstock chemicals, with Guaiacol being the most abundant compound (45.04 mg/mL) among the quantified products by HPLC. Additionally, the system demonstrated high efficiency in anodic wastewater treatment, achieving BOD and COD removal rates ranging from 67.68 % to 72.55 %. This method showcases the use of a sustainable system in combination with a selective catalyst to produce valuable products from usually discarded Kraft lignin while simultaneously treating wastewater.

Bio-separation of value-added products from Kraft lignin: A promising two-stage lignin biorefinery via microbial electrochemical technology.

The most ubiquitous aromatic biopolymer in nature, lignin offers a promising foundation for the development of bio-based chemicals with wide-ranging industrial uses attributable to its aromatic structure. Lignin must first be depolymerized into smaller oligomeric and monomeric units at the initial stage of lignin bioconversion, followed by separation to recover valuable products. This study demonstrates an integrative biorefinery idea based on in-situ depolymerization of the lignin via microbial electro-Fenton reaction in a microbial peroxide-producing cell and recovery of the identified products i.e., phenolic or aromatic monomers by one step high throughput chromatography. The yield percentage of acetovanillone, ethylvanillin, and ferulic acid recovered from the depolymerized lignin using the integrative biorefinery strategy were 2.1 %, 9.1 %, and 9.04 %, respectively. These products have diverse industrial usage and can be employed as platform chemicals. The development of a novel system for efficient simultaneous lignin depolymerization and subsequent quality separation are demonstrated in this study.

In-situ degradation of Amphotericin B in a microbial electrochemical cell containing wastewater.

Antimicrobial resistance raises serious medical implications and is primarily caused by indiscriminate usage and environmental contamination with antimicrobial agents. To prevent microbes from developing resistance against antimicrobial agents, they must be effectively degraded. This is the first study that investigates the degradation of Amphotericin B(AmB) with simultaneous wastewater treatment in a Microbial Peroxide producing cell (MPPC). Two sets of MPPCs (A and B) were used to degrade AmB oxidatively, one with H2O2 and the other with the microbial electro Fenton process in a catholyte containing 0.1% AmB. MPPC A and B had voltage outputs of 0.356 ± 3 V and 0.411 ± 2 V, producing 26 ± 0.04 mM and 44 ± 0.8 mM of H2O2 respectively. The structural changes of treated samples were analyzed using Fourier Transformed Infrared Spectroscopy, which revealed the disappearance of major characteristic bands such as the NH band (1556 cm-1), the CH band Polyene ring (3358 cm-1), and others, implying the disruption of multiple double bonds in polyene, resulting in the structure's lactone ring breakdown. Liquid chromatography quadrupole time-of-flight revealed the changes in retention time and peak area of treated samples in comparison to native AmB which also confirmed its structural changes. Such structural disruption induced the drug to lose its antifungal action since no zones of inhibition were detected in an antimicrobial susceptibility test against Candida albicans. The degradation of 57.05% and 69.83% of AmB by H2O2 and the Fenton process was also correlated with a reduction in COD. Simultaneously the anodic wastewater treatment in both the MPPCs had COD removal efficiency of 78% and 82% and the BOD removal efficiency was 75.38% and 90% respectively. The MPPC system's process conditions and reactor design could be optimized further to enhance antimicrobial degradation and wastewater treatment. This research offers a sustainable and efficient method for expediting antimicrobial degradation while simultaneously treating wastewater.