Assuntos
Betacoronavirus/isolamento & purificação , Infecções por Coronavirus , Pulmão/diagnóstico por imagem , Pandemias , Administração dos Cuidados ao Paciente/métodos , Pneumonia Viral , Radiografia Torácica/métodos , Tomografia Computadorizada por Raios X/métodos , COVID-19 , Teste para COVID-19 , Técnicas de Laboratório Clínico/métodos , Infecções por Coronavirus/sangue , Infecções por Coronavirus/diagnóstico , Infecções por Coronavirus/fisiopatologia , Infecções por Coronavirus/terapia , Humanos , Masculino , Pessoa de Meia-Idade , Pneumonia Viral/sangue , Pneumonia Viral/diagnóstico , Pneumonia Viral/fisiopatologia , Pneumonia Viral/terapia , SARS-CoV-2 , Avaliação de Sintomas/métodos , Resultado do TratamentoRESUMO
No abstract available.
RESUMO
Intra-disc injection of mesenchymal stem cells (MSCs) to treat disc degeneration may lead to unfavorable complications, particularly osteophyte formation. Development of an effective method to block the injection portal, prevent the leakage of injected cells and materials and, hence, prevent osteophyte formation is of the utmost importance before MSC-based therapies can be applied in a clinical setting. Here we seek to alleviate the cell leakage problem and the associated complication osteophyte formation by developing an injectable annulus plug to block the injection portal during intra-disc delivery. Specifically, we fabricated a needle-shaped collagen plug by photochemical crosslinking and successfully delivered it intra-discally, in association with MSCs in collagen microsphere carriers, using a custom-made delivery device. The mechanical performance of the plug and its effectiveness in reducing cell leakage were evaluated ex vivo under compression and in torsion push-out tests. The results demonstrate that the plug survived physiologically relevant loadings and significantly reduced leakage and enhanced retention of the injected materials. Finally, a pilot in vivo study in rabbits was conducted to evaluate the performance of the plug. Microcomputed tomography imaging and histology revealed that the plug significantly reduced osteophyte formation. This work suggests the potential of the annulus plug as an adjunct or annulus closure device for intra-disc delivery of cells and materials.
Assuntos
Colágeno/administração & dosagem , Extravasamento de Materiais Terapêuticos e Diagnósticos/etiologia , Extravasamento de Materiais Terapêuticos e Diagnósticos/prevenção & controle , Degeneração do Disco Intervertebral/cirurgia , Transplante de Células-Tronco Mesenquimais/efeitos adversos , Transplante de Células-Tronco Mesenquimais/métodos , Animais , Colágeno/efeitos da radiação , Reagentes de Ligações Cruzadas/efeitos da radiação , Estudos de Viabilidade , Degeneração do Disco Intervertebral/complicações , Luz , Teste de Materiais , Fotoquímica/métodos , CoelhosRESUMO
The influence of the structure of C6 growth substrate on the activity of oxidoreductases and content of cytochromes b5 and P-450 in cells of Pseudomonas aeruginosa and P. fluorescens was studied. Among the hydrocarbons tested (hexane, 1-hexene, cyclohexene), 1-hexene was the most efficient inducer of the synthesis of cytochromes b5 and P-450 in log-phase cells of the pseudomonads. Substitution of the hydrocarbon substrate for a carbohydrate one (glucose) also resulted in a considerable increase in the activity of enzymes of the NADH-dependent electron-transport chain.