Quantitative assessment of AD markers using naked eyes: point-of-care testing with paper-based lateral flow immunoassay.

Aß42 is one of the most extensively studied blood and Cerebrospinal fluid (CSF) biomarkers for the diagnosis of symptomatic and prodromal Alzheimer's disease (AD). Because of the heterogeneity and transient nature of Aß42 oligomers (Aß42Os), the development of technologies for dynamically detecting changes in the blood or CSF levels of Aß42 monomers (Aß42Ms) and Aß42Os is essential for the accurate diagnosis of AD. The currently commonly used Aß42 ELISA test kits usually mis-detected the elevated Aß42Os, leading to incomplete analysis and underestimation of soluble Aß42, resulting in a comprised performance in AD diagnosis. Herein, we developed a dual-target lateral flow immunoassay (dLFI) using anti-Aß42 monoclonal antibodies 1F12 and 2C6 for the rapid and point-of-care detection of Aß42Ms and Aß42Os in blood samples within 30 min for AD diagnosis. By naked eye observation, the visual detection limit of Aß42Ms or/and Aß42Os in dLFI was 154 pg/mL. The test results for dLFI were similar to those observed in the enzyme-linked immunosorbent assay (ELISA). Therefore, this paper-based dLFI provides a practical and rapid method for the on-site detection of two biomarkers in blood or CSF samples without the need for additional expertise or equipment.

Elucidating the Trade-off between Membrane Wetting Resistance and Water Vapor Flux in Membrane Distillation.

Membrane distillation (MD) has been receiving considerable attention as a promising technology for desalinating industrial wastewaters. While hydrophobic membranes are essential for the process, increasing membrane surface hydrophobicity generally leads to the reduction of water vapor flux. In this study, we investigate the mechanisms responsible for this trade-off relation in MD. We prepared hydrophobic membranes with different degrees of wetting resistance through coating quartz fiber membranes with a series of alkylsilane molecules while preserving the fiber structures. A trade-off between wetting resistance and water vapor flux was observed in direct-contact MD experiments, with the least-wetting-resistant membrane exhibiting twice as high vapor flux as the most wetting-resistant membrane. Electrochemical impedance analysis, combined with fluorescence microscopy, elucidated that a lower wetting resistance (still water-repelling) allows deeper penetration of the liquid-air interfaces into the membrane, resulting in an increased interfacial area and therefore a larger evaporative vapor flux. Finally, we performed osmotic distillation experiments employing anodized alumina membranes that possess straight nanopores with different degrees of wetting resistance, observed no trade-off, and substantiated this proposed mechanism. Our study provides a guideline to tailor the membrane surface wettability to ensure stable MD operations while maximizing the water recovery rate.

Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor.

Salmonella, a common foodborne pathogen, causes many cases of foodborne illness and poses a threat to public health worldwide. Immunological detection systems can be combined with nanoparticles to develop sensitive and portable detection technologies for timely screening of Salmonella infections. Here, we developed an antibody-probe-based immuno-N-hydroxysuccinimide (NHS) bead (AIB) system to detect Salmonella. After adding the antibody probe, Salmonella accumulated in the samples on the surfaces of the immuno-NHS beads (INBs), forming a sandwich structure (INB-Salmonella-probes). We demonstrated the utility of our AIB diagnostic system for detecting Salmonella in water, milk, and eggs, with a sensitivity of 9 CFU mL-1 in less than 50 min. The AIB diagnostic system exhibits highly specific detection and no cross-reaction with other similar microbial strains. With no specialized equipment or technical requirements, the AIB diagnostic method can be used for visual, rapid, and point-of-care detection of Salmonella.

The vacuum ultraviolet beamline/endstations at NSRL dedicated to combustion research.

An undulator-based vacuum ultraviolet (VUV) beamline (BL03U), intended for combustion chemistry studies, has been constructed at the National Synchrotron Radiation Laboratory (NSRL) in Hefei, China. The beamline is connected to the newly upgraded Hefei Light Source (HLS II), and could deliver photons in the 5-21 eV range, with a photon flux of 10(13) photons s(-1) at 10 eV when the beam current is 300 mA. The monochromator of the beamline is equipped with two gratings (200 lines mm(-1) and 400 lines mm(-1)) and its resolving power is 3900 at 7.3 eV for the 200 lines mm(-1) grating and 4200 at 14.6 eV for the 400 lines mm(-1) grating. The beamline serves three endstations which are designed for respective studies of premixed flame, fuel pyrolysis in flow reactor, and oxidation in jet-stirred reactor. Each endstation contains a reactor chamber, an ionization chamber where the molecular beam intersects with the VUV light, and a home-made reflectron time-of-flight mass spectrometer. The performance of the beamline and endstations with some preliminary results is presented here. The ability to detect reactive intermediates (e.g. H, O, OH and hydroperoxides) is advantageous in combustion chemistry research.

[Multi-Element Detection in Molten Steel with Laser-Induced Breakdown Spectroscopy].

On-line element content detection in iron and steel industry is one of the key techniques to ensure the quality in iron and steel metallurgy. Laser Induced Breakdown Spectroscopy (LIBS) has been applied to on-line components detection in molten steel. We have built LIBS system for components detection of molten steel in laboratory. The system consists of a Q-switched Nd∶YAG laser (repetition rate 10 Hz, wavelength 1 064 nm, pulse length 10 ns, pulse energy about 120 mJ), high frequency induction furnace (temperature 1 600 ℃), spectrometer (wavelength range 186～310 nm, spectral resolution 0.1 nm), laser focusing and spectral signal collecting system. Multi-elements were detected in molten steel with the application of deep-UV detector coating and solarization resistant fibers. According to the calibration curves of C, S, Mn and Cr, the limit of detections are 169, 15, 58.9 and 210 µg·g-1 respectively. The R-squares of calibration curves of C, S, Mn, and Cr are better than 0.96 by using appropriate analytical lines and reference lines. At the same time, through the comparison of different elements, we find the best calibration curve of different element need different delay conditions.

[Development of a High Spectral Resolution UV Flat-Field Spectrograph].

As an important optical splitting element, grating is used in many different spectrometers and spectrographs. Spherical varied-line-spacing grating (SVLSG) is easily combined with array detectors to get a wide wavelength range of spectrums in one time, because it can focus the spectrums in approximately a plane. Therefore, it's widely used in many spectral instruments. We usually only know the central groove density of a commercial grating and its mounting parameters, while its line spacing parameters are unknown. Moreover, the mounting parameters are optimized within the whole using wavelength range of the grating. However, in most circumstances only part of the wavelength range is used. Therefore, the mounting parameters are not optimized for the needed wavelength range. Under this condition, in this article we developed a method based on the focusing theory of the flat-field grating and the mounting parameters the manufacture provided to deduce the line spacing parameters of the grating. With these parameters, we can optimize the detector position according to the wavelength range we need and ray tracing can be done to test the optical system. In this article we developed a high spectral resolution ultraviolet spectrograph, covering a wavelength range of 230-280 nm. The grating used in this spectrograph has a central groove density of 1 200 lines x mm(-1) and a designed wavelength range of 170-500 nm. We deduced the line spacing parameters of the grating and optimized the detector mounting parameters. Hollow cathode lamps of different elements were used to calibrate the spectrograph and test the spectral resolution of it. Wavelength calibration of the spectrograph has been done with the parameter fitting method, and the calibration accuracy is better than 0.01 nm. Results show the spectral resolution of the spectral graph is about 0.08 nm at 280.20 nm.

[The auto-focusing remote laser-induced breakdown spectroscopy system].

The present paper presents an auto-focus laser-induced breakdown spectroscopy (LIBS) remote measuring system. This system contains a Schwarzschild telescope, which consists of a convex mirror and a concave mirror. The two spherical mirrors are coaxially placed. The convex mirror is mounted on a motorized linear translation stage. With this motorized linear translation stage, the convex mirror can move along the optical axis to change the spacing between the convex mirror and the concave mirror. Therefore the focal length can be adjusted to focus the laser on samples at different distances and collect the plasma spectra. The advantages of the telescope system include, firstly, the light path of laser focusing and spectra signal collection is the same, which make it easier for mounting and collimation; secondly, the light path of the telescope uses total reflection type, which is fit for the detection in ultra-violate region; finally, the telescope consists of only two spherical mirrors which are relatively easier to manufacture. Within the translation range of the motorized linear translation stage, the focal length of the telescope in this paper can be adjusted from 1.5 to 3.6 m. The diameter of the focusing spot varies from 0.5 to 1.0 mm. Utilizing this telescope system, LIBS experiments were conducted using copper sample. And the characteristic lines of Cu element (Cu I 223.01 nm, Cu I 224.43 nm) obtained are used for the auto focusing. By investigating the relation of the area of spectral lines covered and the spacing between the mirrors, the optimal laser focusing location was obtained. The LIBS experiment results show that the system functions well, fulfilling the demand of remote ablation of sample and LIBS spectral measuring, and the telescope is able to auto-focus the laser on samples at different position to perform remote LIBS experiment.

Deciphering molecular heterogeneity and dynamics of human hippocampal neural stem cells at different ages and injury states.

While accumulated publications support the existence of neurogenesis in the adult human hippocampus, the homeostasis and developmental potentials of neural stem cells (NSCs) under different contexts remain unclear. Based on our generated single-nucleus atlas of the human hippocampus across neonatal, adult, aging, and injury, we dissected the molecular heterogeneity and transcriptional dynamics of human hippocampal NSCs under different contexts. We further identified new specific neurogenic lineage markers that overcome the lack of specificity found in some well-known markers. Based on developmental trajectory and molecular signatures, we found that a subset of NSCs exhibit quiescent properties after birth, and most NSCs become deep quiescence during aging. Furthermore, certain deep quiescent NSCs are reactivated following stroke injury. Together, our findings provide valuable insights into the development, aging, and reactivation of the human hippocampal NSCs, and help to explain why adult hippocampal neurogenesis is infrequently observed in humans.

[Laser-induced breakdown spectroscopy system for elements analysis in high-temperature and vacuum environment].

Laser-induced breakdown spectroscopy (LIBS) is one of the most promising technologies to be applied to metallurgical composition online monitoring in these days. In order to study the spectral characters of LIBS spectrum and to investigate the quantitative analysis method of material composition under vacuum and high temperature environment, a LIBS measurement system was designed and set up which can be used for conducting experiments with high-temperature or molten samples in different vacuum environment. The system consists of a Q-switched Nd : YAG laser used as the light source, lens with different focus lengths used for laser focusing and spectrum signal collecting, a spectrometer used for detecting the signal of LIBS spectrums, and a vacuum system for holding and heating the samples while supplying a vacuum environment. The vacuum was achieved and maintained by a vacuum pump and an electric induction furnace was used for heating the system. The induction coil was integrated to the vacuum system by attaching to a ceramic sealing flange. The system was installed and testified, and the results indicate that the vacuum of the system can reach 1X 10(-4) Pa without heating, while the heating temperature could be about 1 600 degreeC, the system can be used for melting metal samples such as steel and aluminum and get the LIBS spectrum of the samples at the same time. Utilizing this system, LIBS experiments were conducted using standard steel samples under different vacuum or high-temperature conditions. Results of comparison between LIBS spectrums of solid steel samples under different vacuum were achieved, and so are the spectrums of molten and solid steel samples under vacuum environment. Through data processing and theoretical analyzing of these spectrums, the initial results of those experiments are in good agreement with the results that are presently reported, which indicates that the whole system functions well and is available for molten metal LIBS experiment under vacuum environment.

[EUV flat field grating spectrometer and performance measurement].

A high-resolution extreme ultraviolet (EUV) spectrometer has been developed to diagnose the magnetically confined plasmas. A holographic spherical varied line spacing concave grating which provides a flat focal plane is used as the diffraction element working with the grazing incidence angle of 3 degrees. The nominal groove density is 1200 lines x mm(-1). A deeply cooled back-illuminated CCD camera is used as the spectra detector and a mechanical shutter is used to control the time of exposure. It covers the wavelength range of 5-50 nm with the CCD cameral moving along the spectra focal plane to cover different wavelength range interested. Spectrometer design is presented and it was tested by a Penning discharge light source. By the wavelength calibration, the actual parameters of the optical system were calculated and the wavelength accuracy is 0.003 nm. Results show that the spectral resolution is about 0.015 nm at 20 nm with the width of entrance slit opened at 30 microm, which agrees with the design goal.

Long-Chain PFASs-Free Omniphobic Membranes for Sustained Membrane Distillation.

Omniphobic membranes possessing high wetting resistance have been created for the treatment of challenging hypersaline feedwaters with low surface tension through membrane distillation (MD). However, virtually all such membranes are fabricated with long-chain per- and polyfluoroalkyl substances (PFASs, ≥8 fluorinated carbons). The environmental risks and high bioaccumulation potential of long-chain PFASs have raised increasing concerns. Developing highly wetting-resistant MD membranes while avoiding the use of long-chain PFASs is essential to improve the viability of MD for resilient and sustainable water purification. We demonstrate that MD membranes with exceptional wetting resistance can be designed through the combination of hierarchically structured membranes consisting of re-entrant texture at different length scales and (ultra)short-chain fluorocarbons, which have lower acute toxicity and bioaccumulation potentials than long-chain PFASs. Our hierarchically structured membrane with three-tier micro/nanostructure fabricated with short-chain fluorocarbon possesses superior wetting resistance, which is comparable to or higher than the long-chain PFASs-based omniphobic membranes reported in the literature. Furthermore, the hierarchically structured membranes fabricated with ultrashort-chain fluorocarbons display improved wetting resistance against feedwaters with low surface tension. Our findings indicate that long-chain PFASs are not required when designing wetting-resistant membranes and that the balance between sustainability and wetting resistance should be tailored to the wetting potential of the feedwater.

The water footprint of hydraulic fracturing under different hydroclimate conditions in the Central and Western United States.

The oil and gas (O&G) exploitation via hydraulic fracturing (HF) has augmented both energy production and water demand in the United States. Despite the geographical coincidence of U.S. shale plays with water-scarce areas, the water footprint of HF under drought conditions, as well as its impacts on local water allocation, have not been well understood. In this study, we investigated the water consumption by HF activities under different hydroclimate conditions in eleven O&G-producing states in the central and western U.S. from 2011 to 2020. Our results show that the water consumption under abnormally dry or drought climates accounted for 49.7 % (475.3 billion gallons or 1.8 billion m3) of total water usage of HF, with 9 % (86.1 billion gallons or 325.9 million m3) of water usage occurring under extreme or exceptional drought conditions. The water usage of HF under arid conditions can translate to high densities of water footprint at the local scale, equivalent to >10 % and 50 % of the annual water usage by the irrigation and domestic sectors in 6-29 irrigation-active counties and 11-51 counties (depending on the specific year), respectively. Such water stress imposed by O&G production, however, can be effectively mitigated by the reuse of flowback and produced water. Our findings, for the first time, quantify the water footprint of HF as a function of hydroclimate condition, providing evidence that the water consumption by HF intensifies local water competition and alters water supply threatened by climate variability. This renders wastewater reuse necessary to maintain water sustainability of O&G-producing regions in the context of both a rising O&G industry and a changing climate.

Assessment of the invisible blood contamination on nurses' gloved hands during vascular access procedures in a hemodialysis unit.

A prospective study was conducted to assess potential invisible blood contamination on nurses' gloved hands during vascular access procedures using the occult blood detection method in a hemodialysis unit. 60.13% (273/454) of samples tested positive for hemoglobin. These results highlighted the importance of hand hygiene and glove change during hemodialysis access care.

BRN2 as a key gene drives the early primate telencephalon development.

Evolutionary mutations in primate-specific genes drove primate cortex expansion. However, whether conserved genes with previously unidentified functions also play a key role in primate brain expansion remains unknown. Here, we focus on BRN2 (POU3F2), a gene encoding a neural transcription factor commonly expressed in both primates and mice. Compared to the limited effects on mouse brain development, BRN2 biallelic knockout in cynomolgus monkeys (Macaca fascicularis) is lethal before midgestation. Histology analysis and single-cell transcriptome show that BRN2 deficiency decreases RGC expansion, induces precocious differentiation, and alters the trajectory of neurogenesis in the telencephalon. BRN2, serving as an upstream factor, controls specification and differentiation of ganglionic eminences. In addition, we identified the conserved function of BRN2 in cynomolgus monkeys to human RGCs. BRN2 may function by directly regulating SOX2 and STAT3 and maintaining HOPX. Our findings reveal a previously unknown mechanism that BRN2, a conserved gene, drives early primate telencephalon development by gaining novel mechanistic functions.

A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo.

The development of biosensors capable of achieving accurate and precise molecular measurements in the living body in pH-variable biological environments (e.g. subcellular organelles, biological fluids and organs) plays a significant role in personalized medicine. Because they recapitulate the conformation-linked signaling mechanisms, electrochemical aptamer-based (E-AB) sensors are good candidates to fill this role. However, this class of sensors suffers from a lack of a stable and pH-independent redox reporter to support their utility under pH-variable conditions. Here, in response, we demonstrate the efficiency of an electron donor π-extended tetrathiafulvalene (exTTF) as an excellent candidate (due to its good electrochemical stability and no proton participation in its redox reaction) of pH-independent redox reporters. Its use has allowed improvement of E-AB sensing performance in biological fluids under different pH conditions, achieving high-frequency, real-time molecular measurements in biological samples both in vitro and in the bladders of living rats.

[Measuring method of spatial and spectral distribution of hollow cathode lamp].

In the present paper, the authors propose a method to measure the spatial and spectral distribution of hollow cathode lamp by imaging spectrometer. A push-broom convex grating imaging spectrometer based on Offner system is presented, with wavelength range 400-1 000 nm, field angle 22 degrees and spectral resolution 2-3 nm. The spatial distribution of Hg hollow cathode lamp was measured for the first time; this gives the spatial intensity distribution for different wavelengths and spectral distribution at different positions, with high spatial and spectral resolution. Also the hyperspectral data under different working current condition was obtained in order to analyze the differences of the distribution. It will be a powerful tool for the spatial and spectral properties measurement of hollow cathode lamps and other kinds of light sources.

Effect of continuous nursing care based on the IKAP theory on the quality of life of patients with chronic obstructive pulmonary disease: A randomized controlled study.

OBJECTIVE: To explore the effect of continuous nursing care based on the Information, Knowledge, Attitude, and Practice (IKAP) theory on the quality of life of patients with chronic obstructive pulmonary disease (COPD). METHODS: This study is a randomized control trial. COPD patients attending the Affiliated Hospital of Inner Mongolia Medical University, China between July 1 and October 31, 2017 were eligible. Following random assignment of participants to either the intervention group or control group, 70 patients (35 in each group) were included in the final sample. The intervention group received nursing care based on the Information, Knowledge, Attitude, and Practice theory, while the control group received standard nursing care. Data were collected before the intervention, 1 month after the intervention, and three months after the intervention. The St. George's Respiratory Questionnaire (SGRQ) was used to measure quality of life. RESULTS: Three months after the intervention, there were significant differences in the total SGRQ score (20.29 ±â€Š10.03 vs 30.14 ±â€Š12.52) and in the three SGRQ dimensions between the intervention group and the control group (P < .05). A repeated-measures analysis of variance showed that the total SGRQ score and the scores for impact and symptoms had a significant time effect (P < .001), that the total SGRQ score and the score for symptoms had a significant interaction effect (P < .05), and that the impact dimension had a significant group effect (P = .042). Pairwise comparisons of the data for the intervention group showed that there were significant differences between the pre-intervention and 1 month after intervention scores as well as between pre-intervention and three months after intervention, for the total SGRQ scores and the scores for impact and symptoms(P < .001). In terms of the impact dimension, there was a significant difference in the intervention group between 1 month after intervention and 3 months after intervention (P = .016). CONCLUSION: Continuous nursing care based on Information, Knowledge, Attitude, and Practice theory improved quality of scores at 3 months after intervention among COPD patients. Given limitations of the study, future large-scale studies are needed to validate our results.

Development and Application of an Immunocapture PCR Diagnostic Assay Based on the Monoclonal Antibody for the Detection of Shigella.

BACKGROUND: Shigella is among the most important human pathogenic microorganisms, infecting both humans and nonhuman and causing clinically severe diarrhea. Shigella must be enriched before detection, which is time-consuming. OBJECTIVES: To develop a sensitive, rapid, and specific method for Shigella detection. MATERIALS AND METHODS: Shigella was used as an antigen to generate monoclonal antibodies (mAbs). mAbs were screened via indirect enzyme-linked immunosorbent assay (ELISA) and western blot, and two mAbs were selected. The mAb A3 showed high affinity and specificity and was used to develop immune magnetic beads (IMBs) for Shigella enrichment. An immunocapture (IC)-PCR primer was designed from the ipaH gene, and IC-PCR was developed based on the IMBs and PCR. RESULTS: This system shortened the Shigella detection time to 70 min. The sensitivity of the IC-PCR was 9 colony-forming units.mL-1 in artificial milk. The accuracy of the IC-PCR was confirmed using 46 clinical samples collected from monkeys. The IC-PCR results were consistent with the serological and biochemical assays. CONCLUSION: The IC-PCR described herein accurately detected Shigella from milk samples, monkeys and can thus be used to complement classical detection methods.

Factors associated with post-traumatic stress disorder of nurses exposed to corona virus disease 2019 in China.

Quantitative studies using validated questionnaires on post-traumatic stress disorder (PTSD) of Nurses exposed to corona virus disease 2019 (COVID-19) in China are rare and the baseline PTSD must first be evaluated before prevention. This study aimed to investigate the factors potentially involved in the level of PTSD of Nurses exposed to COVID-19 in China.In this cross-sectional study, male and female Nurses (n = 202) exposed to COVID-19 from HuBei China were included in the final sample. The PTSD Checklist-Civilian (PCL-C) questionnaire and Simplified Coping Style Questionnaire (SCSQ) were used for evaluation. Multivariate stepwise linear regression analysis and spearman correlation test were performed to assess the association between various factors associated with PTSD.The incidence of PTSD in Nurses exposed to COVID-19 was 16.83%, the PCL-C score was 27.00 (21.00-34.00), and the highest score in the three dimensions was avoidance dimension 9.50 (7.00-13.25); multivariable stepwise linear regression analysis showed that job satisfaction and gender were independently associated with lower PCL-C scores (both P < .001); PCL-C scores were correlated with positive coping (r = -0.151, P = .032), negative coping (r = 0.154, P = .029).Nurses exposed to COVID-19 from HuBei China with job satisfaction, male and positive coping had low PCL-C scores which necessitate reducing the PTSD level by ways of improving job satisfaction, positive response, and strengthening the psychological counseling of female nurses in order to reduce the risk of psychological impairment.