RESUMO
OBJECTIVES: To observe the expression changes of hypoxia inducible factor-1αï¼HIF-1αï¼ and vascular endothelial growth factor-A ï¼VEGF-Aï¼ in rats with arrhythmias, and to explore the differences of the expression pattern in the two indicators of acute myocardial ischemia caused by arrhythmias and coronary insufficiency. METHODS: The arrhythmia was induced by CaCl2, and the expression changes of HIF-1α and VEGF-A were detected by immunohistochemistry, Western blotting and real-time PCR within 6 h after the arrhythmia in rats. RESULTS: The expression of HIF-1α and VEGF-A showed diffuse in the myocardial tissue of rats died from arrhythmias. Both of them increased in the early arrhythmia, then decreased. Extensive myocardial ischemia happened at the beginning of arrhythmia occurrence and its range didn't expand with time. CONCLUSIONS: The expressions of HIF-1α and VEGF-A in myocardium of the rats with arrhythmia can provide evidence for the differential diagnosis of acute myocardial ischemia caused by fatal arrhythmia and coronary insufficiency.
Assuntos
Arritmias Cardíacas/metabolismo , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Isquemia Miocárdica/metabolismo , Miocárdio/metabolismo , Fator A de Crescimento do Endotélio Vascular/metabolismo , Animais , RatosRESUMO
A retrospective study was carried out in 406 cases to analyse ultrasound feature of ovarian tumor and compared with pathological specimen. The aim is to suggest a new classification of ovarian tumors in ultrasonography. According to the pathological reports the ovarian tumors were divided into five groups, and found out the ultrasonographic characteristic feature of benign or malignant. The results showed that the new classification of ovarian tumor has a better method. The diagnostic accurate rate is 90.9%. In addition, we described the characteristic feature of five groups of ovarian tumors by sonography and discussed the role in early diagnosis of malignant ovarian tumors and improvement five year survival rate.