Microdose Lithium Treatment Reduced Inflammatory Factors and Neurodegeneration in Organotypic Hippocampal Culture of Old SAMP-8 Mice.

It was already shown that microdoses of lithium carbonate (Li2CO3) promoted memory stabilization in humans and mice. Prolonged treatment also reduced neuronal loss and increased the density of the neurotrophin BDNF in transgenic mice for Alzheimer's disease. The aim of this study was to evaluate whether lithium ions affect inflammatory profiles and neuronal integrity in an animal model of accelerated senescence (SAMP-8). Organotypic hippocampal cultures obtained from 11 to 12-month-old SAMP-8 mice were treated with 2 µM, 20 µM and 200 µM Li2CO3. 2 µM Li2CO3 promoted a significant reduction in propidium iodide uptake in the CA2 area of hippocampus, whereas 20 µM promoted neuroprotection in the CA3 and GrDG areas. 200 µM caused an increase in cellular death, showing toxicity. Measured with quantitative PCR, IL-1α, IL-6 and MIP-1B/CCL-4 gene expression was significantly reduced with 20 µM Li2CO3, whereas IL-10 gene expression was significantly increased with the same concentration. In addition, 2 µM and 20 µM Li2CO3 were also effective in reducing the activation of NFkB and inflammatory cytokines densities, as evaluated by ELISA. It is concluded that very low doses of Li2CO3 can play an important role in neuroprotection as it can reduce neuronal loss and neuroinflammation in older individuals.

Neuroprotective Effects of Kinin B2 Receptor in Organotypic Hippocampal Cultures of Middle-Aged Mice.

Aging is a multifactorial phenomenon that results in several changes at cellular and molecular levels and is considered the main risk factor for some neurodegenerative diseases. Several evidence show the participation of the kallikrein-kinin system (KKS) in neurodegeneration and this system has been associated with inflammation and immunogenic responses in the central and peripheral systems by the activation of the B1 and B2 receptors. Previous work by our group showed that bradykinin (BK) and the B2 receptor played a possible role in neuroprotection. Therefore, the objective of this study was to evaluate the participation of B2 receptors in cell viability, neuroinflammatory response and neuroplasticity in organotypic hippocampal cultures (OHCs) of 6- and 12-month-old mice. It was observed that activation of the B2 receptor by bradykinin decreased the inflammatory response and increased plasticity in 12-month-old slices. Conversely, there was an increase in the inflammatory response and a decrease in neural plasticity in the 6-month-old slices. In both ages, an increase in cell viability was observed. This data suggests that the function of the kinin B2 receptor in the hippocampus is modulated by age, providing neuroprotective action in old age.

Genotoxic and mutagenic evaluation of water samples from a river under the influence of different anthropogenic activities.

Pollution of aquatic ecosystems is associated with the discharge of mostly industrial and urban effluents, which may cause loss of biodiversity and damage to public health. This study aims to evaluate the toxicity and mutagenicity of water samples collected in the Corrente River, a major waterway in the river basin district of Pedro II, Piauí (Brazil). This river is exposed to intense anthropogenic influence from urban, automotive mechanical and family farm waste, and it is used as the main source of water supply by the population. Water samples were collected during the rainy and dry seasons, at four sites in the Corrente River, and evaluated by physicochemical, microbiological and inorganic elements analyses. The samples were evaluated for mutagenicity using the Allium cepa test (toxicity, chromosomal aberration and micronucleus tests) and fish (Tilapia rendalli and Hoplias malabaricus). The physicochemical, microbiological and inorganic results show a large contribution to the pollution loads at collection points in the town of Pedro II, demonstrating the influence of urban pollution. The Al, Si, Ti, Cr, Ni and Cu contents were determined by PIXE. These same Corrente River water samples demonstrated mutagenic effect for A. cepa and fish, as well as toxicity in the A. cepa test. The observations of mutagenic effect may suggest that the complex mixture of agents is comprised of both clastogenic and aneugenic agents. This study also showed the need for constant monitoring in places with environmental degradation caused by urban sewage discharges.