RESUMO
OBJECTIVE: To identify clinical, laboratory and morphological prognostic criteria at different during Crohn's disease in children. MATERIALS AND METHODS: in 48 patients with primary BC in age from 1 year to 18 years retrospectively evaluated the clinical and medical history, laboratory parameters, used complex morphological study of the intestinal mucosa. RESULTS: prediction of complicated Crohns diseaseis important to the high humoral activity, small bowel localization process with the identifcation of ulcers, fractures, the presence of such cell populations in the mucosa of the colon as inactive macrophages and young tbroblasts, muscle infrtration of neutrophilic granulocytes plate. CONCLUSION: in the primary treatment of the patient along with the production of BC diagnosis can predict the course of the disease and determine the tactics of the patient.
Assuntos
Colo/patologia , Doença de Crohn , Mucosa Intestinal/patologia , Intestino Delgado/patologia , Adolescente , Criança , Pré-Escolar , Doença de Crohn/diagnóstico , Doença de Crohn/patologia , Feminino , Humanos , Lactente , Masculino , Prognóstico , Estudos RetrospectivosRESUMO
BACKGROUND: The aim of the study was to investigate the levels of S-nitrosothiols (RSNO) in blood serum of children with inflammatory bowel disease (IBD), nonspecific ulcerative colitis (NUC) and Crohn's disease (CD), and correlate the obtained data with the number of total nitric oxide metabolites (NOx) and proinflammatory cytokines (PC). METHODS: RSNO and NOx were determined by the Griess method and PC by ELISA. We examined 174 children of both genders aged from 6 to 17 years (mean age - 14 years) including 112 children suffering from IBD and 62 children from the control group. The results were statistically processed using Statistica 6.1. RESULTS: The obtained data show an increased but statistically indistinguishable RSNO number in NUC and CD. We revealed the absence of correlation between RSNO and NO and unidirectional RSNO and PC values. CONCLUSIONS: The obtained data of RSNO concentrations in blood serum of children with IBD suggest the participation of RSNO in IBD pathogenesis in children and an indirect correlation of the translation of nitric oxide synthase aberrant activity into RSNO.
Assuntos
Citocinas/sangue , Mediadores da Inflamação/sangue , Doenças Inflamatórias Intestinais/sangue , Óxido Nítrico/sangue , S-Nitrosotióis/sangue , Adolescente , Estudos de Casos e Controles , Criança , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , MasculinoRESUMO
UNLABELLED: RELEVANCE OF RESEARCH: Inflammatory bowel diseases are among the most severe pathologies in pediatric gastroenterology, often lead to disability. Despite the similarity of the clinic, pathogenetic mechanisms, the question of the differential diagnosis of ulcerative colitis and Crohn's disease is relevant in view of their different prognosis and treatment strategy. In recent years, in medical uses infrared spectroscopy of blood serum for the differential diagnosis of various inflammatory diseases, benign and malignant tumors. Besides finding increasing application of mathematical methods for data processing, the so-called mathematical modeling of pathological processes, allowing objectify the survey results for a more accurate diagnosis and prognosis of pathological processes. OBJECTIVE: improving the differential diagnosis of ulcerative colitis and Crohn's disease in children. PATIENTS: 21 children with ulcerative colitis, 56 children with Crohn's disease and 34 healthy children. The method of infrared spectroscopy of serum and mathematical modeling results through multivariate entropy analysis. RESULTS: the obtained spectral characteristics of blood serum in children with ulcerative colitis and Crohn's disease and in healthy children, as well as "images of diseased in these pathologies". CONCLUSION: The use of this medical technology reduced the time of diagnosis, which contributes to the timely choice of rational treatment strategies and provides an opportunity to avoid the development of complications, worsening of the disease.
Assuntos
Colite Ulcerativa/diagnóstico , Doença de Crohn/diagnóstico , Espectrofotometria Infravermelho/métodos , Criança , Diagnóstico Diferencial , Feminino , Humanos , Masculino , Modelos Teóricos , Reprodutibilidade dos TestesRESUMO
UNLABELLED: Inflammatory bowel disease is one of the most difficult groups of pathologies in pediatric gastroenterology, due to the steady growth of their prevalence, offset debut at an earlier age, the increase in the incidence of severe complicated forms. Established clinical, genetic, serologic criteria for progression of ulcerative colitis are contradictory, and some--the reach of most medical institutions. PURPOSE: to evaluate the prognostic significance of morphological features of ulcerative colitis relapse and continuous flow. MATERIALS AND METHODS: a retrospective study conducted histological kolonobioptatov 26 children with recurrent, 20--with a continuous flow of ulcerative colitis during their primary treatment in a hospital, the comparison group consisted of 50 children without inflammatory bowel disease. RESULTS: morphological characteristics identified predictors of recurrent ulcerative colitis in the form of severe eosinophilic infiltration of the epithelium, lamina propria of the mucosa. For a continuous flow was characterized: high density cell infiltration of the lamina propria mucosa epithelium predominantly under represented plasma cells, fibroblasts, sclerosis of the basal membrane, the presence of epithelial arcades deformed crypt abscesses. CONCLUSION: the extended study of biopsy material, including assessment of the composition and location of infiltration, fibroblast pool of epithelial-connective relationships in the colonic mucosa of children with ulcerative colitis will not only assess the inflammatory process, but also to predict the course of disease, thereby defining a long-term tactics treatment.
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Colite Ulcerativa/patologia , Colo/patologia , Mucosa Intestinal/patologia , Biópsia , Criança , Progressão da Doença , Seguimentos , Humanos , Prognóstico , Recidiva , Estudos RetrospectivosRESUMO
In this study we report the experience with the provision of rehabilitative services for the children presenting with long-standing chronic diseases of the digestive organs (ulcerative colitis, Crohn's disease, celiac disease) based at a regional health resort. The possibility of comprehensive rehabilitation was substantiated with the contribution by non-medical specialists including a psychologist, social worker, and educator. A number of organizational problems related to rehabilitation logistics and scientific support have been successfully solved. Standard programs comprising the mechanisms of comprehensive rehabilitation and methods for the evaluation of its effectiveness are proposed.
Assuntos
Doença Celíaca/reabilitação , Colite Ulcerativa/reabilitação , Doença de Crohn/reabilitação , Estâncias para Tratamento de Saúde/normas , Modalidades de Fisioterapia/organização & administração , Modalidades de Fisioterapia/normas , Adolescente , Criança , Feminino , Humanos , MasculinoRESUMO
Waveform-stabilized laser pulses have revolutionized the exploration of the electronic structure and dynamics of matter by serving as the technological basis for frequency-comb and attosecond spectroscopy. Their primary sources, mode-locked titanium-doped sapphire lasers and erbium/ytterbium-doped fibre lasers, deliver pulses with several nanojoules energy, which is insufficient for many important applications. Here we present the waveform-stabilized light source that is scalable to microjoule energy levels at the full (megahertz) repetition rate of the laser oscillator. A diode-pumped Kerr-lens-mode-locked Yb:YAG thin-disk laser combined with extracavity pulse compression yields waveform-stabilized few-cycle pulses (7.7 fs, 2.2 cycles) with a pulse energy of 0.15 µJ and an average power of 6 W. The demonstrated concept is scalable to pulse energies of several microjoules and near-gigawatt peak powers. The generation of attosecond pulses at the full repetition rate of the oscillator comes into reach. The presented system could serve as a primary source for frequency combs in the mid infrared and vacuum UV with unprecedented high power levels.