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Contracaecum rudolphii (s. l.) is a complex of sibling species of anisakid nematodes having the fish-eating birds belonging to the Family Phalacrocoracidae as final hosts. The great cormorant Phalacrocorax carbo sinensis is parasitized by C. rudolphii A and C. rudolphii B. Adults and L4 specimens of C. rudolphii (s. l.) (N = 3282) were collected in cormorants from brackish and freshwater ecosystems of Central Italy. Third-stage larvae of Contracaecum (N = 882) were obtained from the fish species Dicentrarchus labrax, Anguilla anguilla, Aphanius fasciatus, Atherina boyeri, Leuciscus cephalus, Barbus barbus, and Carassius carassius captured in the same geographical areas of cormorants' standings. Contracaecum rudolphii A and C. rudolphii B were identified by a multilocus genetic approach: allozymes, sequences analysis of the mtDNA cox2, and ITS region of rDNA gene loci. Differential distribution of the two parasite species was observed in different aquatic environments. Contracaecum rudolphii B outnumbered C. rudolphii A in wintering cormorants from freshwater ecosystems; the opposite trend was found in cormorants from brackish water. Analogously, C. rudolphii A larvae were more prevalent in brackish water fish, while C. rudolphii B larvae were found infecting only freshwater fish. The findings seem to confirm that C. rudolphii A and C. rudolphii B would have a life-cycle adapted to brackish and freshwater environments, respectively. A differential feeding behavior of wintering cormorants, the ecology of the infected fish species, and abiotic factors related to early stages of the parasites are supposed to maintain the distinctiveness of the two parasite species' life cycles in the two different aquatic ecosystems.
Assuntos
Ascaridoidea/fisiologia , Doenças das Aves/parasitologia , Doenças dos Peixes/parasitologia , Animais , Ascaridoidea/classificação , Ascaridoidea/genética , DNA Mitocondrial/genética , DNA Ribossômico , Ecossistema , Feminino , Água Doce , ItáliaRESUMO
This study focused on the development of a novel biocomposite material formed by a thermoplastic biodegradable polyester, poly(butylene succinate-co-adipate) (PBSA), and a carbonaceous filler as biochar (BC) derived by the pyrolysis of woody biomass waste. Composites with various BC contents (5, 10, 15, and 20 wt.%) were obtained by melt extrusion and investigated in terms of their processability, thermal, rheological, and mechanical properties. In all the composites, BC lowered melt viscosity, behaving as a lubricant, and enhancing composite extrudability and injection moulding at high temperatures up to 20 wt.% of biochar. While the use of biochar did not significantly change composite thermal stability, it increased its stiffness (Young modulus). Differential scanning calorimeter (DSC) revealed the presence of a second crystal phase induced by the filler addition. Furthermore, results suggest that biochar may form a particle network that hinders polymer chain disentanglement, reducing polymer flexibility. A biochar content of 10 wt.% was selected as the best trade-off concentration to improve the composite processability and cost competitiveness without compromising excessively the tensile properties. The findings support the use of biochar as a sustainable renewable filler and pigment for PBSA. Biochar is a suitable candidate to replace more traditional carbon black pigments for the production of biodegradable and inexpensive innovative PBSA composites with potential fertilizing properties to be used in agricultural applications.
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When properly compatibilized, the blending of polyethylene (PE) and polyamide (PA) leads to materials that combine low prices, suitable processability, impact resistance, and attractive mechanical properties. Moreover, the possibility of using these polymers without prior separation may be a suitable opportunity for their recycling. In this work, the use of an epoxidized waste vegetable oil (EWVO) was investigated as a green compatibilizer precursor (CP) for the reactive blending of a high-density PE (HDPE) with a polyamide-6 (PA6). EWVO was synthesized from waste vegetable cooking oil (WVO) using ion-exchange resin (Amberlite) as a heterogeneous catalyst. HDPE/PA6 blends were produced with different weight ratios (25/75, 75/25, 85/15) and amounts of EWVO (1, 2, 5 phr). Samples with WVO or a commercial fossil-based CP were also prepared for comparison. All the blends were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), rheology, and mechanical tests. In the case of HDPE/PA6 75/25 and 85/15 blends, the addition of EWVO at 2 phr showed a satisfactory compatibilizing effect, thus yielding a material with improved mechanical properties with respect to the blend without compatibilizer. On the contrary, the HDPE/PA6 25/75 ratio yielded a material with a high degree of crosslinking that could not be further processed or characterized. In conclusion, the results showed that EWVO had a suitable compatibilizing effect in HDPE/PA6 blends with high HDPE content, while it resulted in unsuitable for blends with high content of PA6.
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Xiphias gladius is an important fishing resource. The Mediterranean stock is affected by overfishing and is declining. In this light, the aim of this study was to evaluate the cross-talk among metabolism, stress response, immune system and reproduction in immature and mature females, coupling histological and transcriptomic approaches. The transcriptome of livers from 3 immature and 3 mature females was analyzed using the Artificial Intelligence RNA-Seq. For the histological analysis, ovary and liver samples were collected from 50 specimens caught during the reproductive season in the Mediterranean Sea. A total of 750 genes were differentially expressed between the livers. The gene ontologtabey analysis showed 91 upregulated and 161 downregulated biological process GO terms. Instead, the KEGG enrichment analysis revealed 15 enriched pathways. Furthermore, the binding occurring between estrogen receptors and aryl hydrocarbon receptor nuclear translocator, upregulated in mature females, could be liable for the inhibition of detoxification pathway. Indeed, at the histological level, mature females showed a higher density and number of melanomacrophage centers, biomarkers of stress. The present findings reveal the cross-talk among response to environmental stressors, metabolism and reproduction, highlighting that mature females invest a lot of energy in reproduction instead of immune response and detoxification.
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The increase in concrete structures' durability is a milestone to improve the sustainability of buildings and infrastructures. In order to ensure a prolonged service life, it is necessary to detect the deterioration of materials by means of monitoring systems aimed at evaluating not only the penetration of aggressive substances into concrete but also the corrosion of carbon-steel reinforcement. Therefore, proper data collection makes it possible to plan suitable restoration works which can be carried out with traditional or innovative techniques and materials. This work focuses on building heritage and it highlights the most recent findings for the conservation and restoration of reinforced concrete structures and masonry buildings.
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Molecular weight distributions are widely used to evaluate the effects of aging or modifiers in bituminous binders. As with polymers, the most common techniques to obtain the distributions can be subdivided into two main groups, depending on whether or not they use a solvent. In the first group, the dimension of the molecules is evaluated in a diluted unperturbed state, while, in the second, the dimension derives from the bulk, where aggregated or interacting molecules may behave as single entities. However, the calibration curves used in the bulk are tuned in order to homogenize the results derived from the two approaches. This sort of contradiction, plus the high number of experimental uncertainties, suggest that the term "apparent" should be used for both distributions. These aspects are well known in the field of polymers but have received less attention in the case of bitumens, which are even more complex. This paper pinpoints the advantages and disadvantages of the two techniques, thus highlighting the most appropriate use. Bulk methods are preferred when evaluating properties that are strictly dependent on the microstructure, such as the level of aging and the effects of additives or modifiers. Diluted methods should be used when the molecular size matters, such as in quantifying the presence of polymers or rejuvenators. Both techniques should be used for comparative studies only.
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The present study reports a strategy to produce polyhydroxyalkanoates (PHAs) by culturing the marine bacterium Rhodovulum sulfidophilum DSM-1374. The study was carried out by growing the bacterium anaerobically for 720 h under 16/8 light/dark cycle. Two analytical techniques such as proton magnetic nuclear magnetic resonance (1H NMR) and Fourier transform infrared spectroscopy (FT-IR) were used to determine that the polyester produced was poly-3-hydroxybutirate-co-3-hydroxyvalerate (PHBV). This study showed that the excess of lactate and the limitation of N-P nutrients under a light-dark cycle enhanced PHBV synthesis and achieved a PHBV concentration of 330 mg/L in the R. sulfidophilum culture. During the 30 days of bacterial cultivation, the percentage of polymer in the six harvested dry biomasses gradually increased from 13.7% to 23.4%. In addition, the study showed that PHBV synthesis stopped during the 8-h dark phase and restarted in the light. The light-dark cycle study also showed that R. sulfidophilum DSM-1374 can be grown outdoors because the cells are exposed to the natural light-dark cycle.
Assuntos
Fotobiorreatores , Poli-Hidroxialcanoatos , Bactérias/metabolismo , Fotoperíodo , Poliésteres/metabolismo , Poli-Hidroxialcanoatos/metabolismo , Espectroscopia de Infravermelho com Transformada de FourierRESUMO
In this study, non-chlorinated solvents such as cyclohexanone (CYC) and three ionic liquids, (ILs) (1-ethyl-3-methylimidazolium dimethylphosphate, [EMIM][DMP], 1-ethyl-3-methylimidazolium diethylphosphate, [EMIM][DEP] and 1-ethyl-3-methylimidazolium methylphosphite, [EMIM][MP]) were tested to extract polyhydroxyalkanoates (PHAs) from the purple non-sulfur photosynthetic bacterium (PNSB) Rhodovulumsulfidophilum DSM-1374. The photosynthetic bacterium was cultured in a new generation photobioreactor with 4 L of working volume using a lactate-rich medium. The extracted PHAs were characterized using a thermogravimetric analysis, differential scanning calorimetry, infrared spectroscopy, proton nuclear magnetic resonance and gel permeation chromatography. The most promising results were obtained with CYC at 125 °C with an extraction time of above 10 min, obtaining extraction yields higher than 95% and a highly pure poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB-HV) with around 2.7 mol% of hydroxylvalerate (HV). A similar yield and purity were obtained with chloroform (CHL) at 10 °C for 24 h, which was used as the referent solvent Although the three investigated ILs at 60 °C for 4 and 24 h with biomass/IL up to 1/30 (w/w) obtained PHAs strongly contaminated by cellular membrane residues, they were not completely solubilized by the investigated ILs.
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Cystic fibrosis (CF) is a progressive genetic disease caused by mutations in the gene that produces the CF transmembrane conductance regulator (CFTR) protein. The malfunction of the CFTR protein causes a thick buildup of mucus in the lungs that clogs the airways and traps bacteria, thus leading to infections, extensive lung damage and respiratory failure. Micro-delivery systems are currently being investigated as an efficient way to cross the viscous and complex architecture of the CF mucus. In this study, we produced synthetic and natural microparticles (MPs) based on poly(dllactidecoglycolide) (PLGA) or gellan gum through tailored water/oil emulsion procedures. Morphological and physico-chemical characterizations were carried out on both classes of MPs showing particles having diameters within suitable ranges to reach the CF airways. In vitro biocompatibility tests were also performed on both MPs using a human lung cancer cell line (A549) demonstrating that treatment with MPs induces no cytotoxic effects. Both classes of MPs were loaded with a mucolytic agent (Nacetyl cysteine, NAC) and their release kinetics evaluated using high performance liquid chromatography (HPLC). The analysis pointed out that the amount of NAC released from MPs resulted in a dose-dependent increment, with a rapid release kinetic to satisfy the requirement for inducing an early mucus degradation. Finally, mucolytic action of NAC-loaded MPs was evaluated in an artificial sputum model through its rheological analysis obtaining the lowest viscosity profile after the addition of drug-loaded MPs. Taken together, gained results allowed us to select suitable MPs as potential drug targeting platforms having a mucolytic action for CF treatment.
Assuntos
Materiais Biocompatíveis/metabolismo , Fibrose Cística/metabolismo , Muco/metabolismo , Células A549 , Adulto , Proliferação de Células , Cromatografia Líquida de Alta Pressão , Sistemas de Liberação de Medicamentos/métodos , Humanos , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/metabolismo , Escarro/metabolismoRESUMO
Sodium butyrate (SB) is used as an acidifier in animal feed. We hypothesized that supplemental SB impacts gastric morphology and function, depending on the period of SB provision. The effect of SB on the oxyntic and pyloric mucosa was studied in 4 groups of 8 pigs, each supplemented with SB either during the suckling period (d 4-28 of age), after weaning (d 29 to 39-40 of age) or both, or never. We assessed the number of parietal cells immunostained for H+/K+-ATPase, gastric endocrine cells immunostained for chromogranin A and somatostatin (SST) in the oxyntic mucosa, and gastrin-secreting cells in the pyloric mucosa. Gastric muscularis and mucosa thickness were measured. Expressions of the H+/K+-ATPase and SST type 2 receptor (SSTR2) genes in the oxyntic mucosa and of the gastrin gene in the pyloric mucosa were evaluated by real-time RT-PCR. SB increased the number of parietal cells per gland regardless of the period of administration (P < 0.05). SB addition after, but not before, weaning increased the number of enteroendocrine and SST-positive cells (P < 0.01) and tended to increase gastrin mRNA (P = 0.09). There was an interaction between the 2 periods of SB treatment for the expression of H/K-ATPase and SSTR2 genes (P < 0.05). Butyrate intake after weaning increased gastric mucosa thickness (P < 0.05) but not muscularis. SB used orally at a low dose affected gastric morphology and function, presumably in relationship with its action on mucosal maturation and differentiation.
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Ácido Butírico/farmacologia , Suplementos Nutricionais , Mucosa Gástrica/citologia , Mucosa Gástrica/efeitos dos fármacos , Suínos/fisiologia , Ração Animal , Fenômenos Fisiológicos da Nutrição Animal , Animais , Animais Lactentes , Dieta/veterinária , Células Enteroendócrinas/efeitos dos fármacos , Células Enteroendócrinas/metabolismo , Mucosa Gástrica/metabolismo , Gastrinas/genética , Gastrinas/metabolismo , Regulação da Expressão Gênica/efeitos dos fármacos , ATPase Trocadora de Hidrogênio-Potássio/genética , ATPase Trocadora de Hidrogênio-Potássio/metabolismo , Receptores de Somatostatina/genética , Receptores de Somatostatina/metabolismo , DesmameRESUMO
The microbial community in the guts of mammals is often seen as an important potential target in therapeutic and preventive interventions. The aim of the present study was to determine whether enterotoxigenic Escherichia coli (ETEC) F4 infection in young animals might be counteracted by a probiotic treatment with Lactobacillus sobrius DSM 16698. The experiment was conducted in three randomized consecutive replications, each consisting of 16 piglets, and including a control group and an L. sobrius fed group, both experimentally challenged with ETEC. During the entire trial, the animals' health status, body weight, and microbial parameters were monitored periodically. Probiotic supplementation containing L. sobrius significantly reduced the levels of ETEC in the ileum when fed directly to piglets after weaning. In contrast, the number of days when the piglets had an increased faecal water content was significantly higher in the probiotic group. Nevertheless, an improved daily weight gain was also observed in the animals that received probiotic L. sobrius relative to the control fed group. The data indicate that L. sobrius may be effective in the reduction of the E. coli F4 colonization and may improve the weight gain of infected piglets.
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Escherichia coli Enterotoxigênica/fisiologia , Infecções por Escherichia coli/veterinária , Trato Gastrointestinal/microbiologia , Lactobacillus/fisiologia , Doenças dos Suínos/microbiologia , Suínos/crescimento & desenvolvimento , Suínos/microbiologia , Animais , Líquidos Corporais/imunologia , Contagem de Colônia Microbiana , Escherichia coli Enterotoxigênica/crescimento & desenvolvimento , Escherichia coli Enterotoxigênica/isolamento & purificação , Infecções por Escherichia coli/microbiologia , Conteúdo Gastrointestinal/química , Concentração de Íons de Hidrogênio , Ílio/microbiologia , Imunoglobulina A/análise , Imunoglobulina A/sangue , Probióticos/administração & dosagem , Suínos/imunologia , Doenças dos Suínos/prevenção & controleRESUMO
OBJECTIVE: Our aim was to study the possible synergic action of one prebiotic with increasing dietary doses of a probiotic strain of Bifidobacterium animalis on the translocation of bifidobacteria and on Toll-like receptor (TLR) gene expression in different organs of weaned piglets. METHODS: Sixty-four pigs, reared from 21 to 35 d of age, were fed eight different diets according to a 2 x 4 factorial design: a control diet or the control diet supplemented with three different levels of B. animalis (10(7), 10(9), 10(11) colony-forming units/d), crossed with 0% or 2% sugar beet fructo-oligosaccharides. Pigs were then sacrificed, and the jejunum mucosa, ileocecal lymph nodes, and liver were sampled to determine the presence of Bifidobacterium spp. DNA and to quantify the expression of TLR2-, TLR4-, and tumor necrosis factor-alpha-encoding genes. RESULTS: We found Bifidobacterium spp. genus-specific DNA in lymph nodes of subjects from all dietary treatments, including the control diet, but it increased with the bifidobacteria oral dose (P = 0.065). The linear effect of the dose of B. animalis on the expression of the TLR2-encoding gene in the lymph nodes was observed when fructo-oligosaccharides were added to the diet (P < 0.05). Tumor necrosis factor-alpha-encoding gene expression was positively correlated with TLR4- and TLR2-encoding gene expressions (P < 0.001 and P < 0.01, respectively) and negatively correlated with bifidobacteria DNA (P < 0.05). Moreover, the expression of the TLR4-encoding gene showed a positive correlation with TLR2-encoding gene expression (P < 0.001). In contrast, there was no correlation between expressions of the TLR2- and TLR4-encoding genes with the bifidobacteria DNA. CONCLUSION: Soon after weaning, the translocation of the commensal bacteria in the ileocecal lymph nodes is a physiologic process. Moreover, diet affects the expression of the TLR2-encoding gene.
Assuntos
Translocação Bacteriana/efeitos dos fármacos , Bifidobacterium/crescimento & desenvolvimento , Oligossacarídeos/farmacologia , Probióticos/farmacologia , Receptores Toll-Like/genética , Animais , Animais Recém-Nascidos , Translocação Bacteriana/fisiologia , Contagem de Colônia Microbiana , DNA Bacteriano/análise , Regulação Bacteriana da Expressão Gênica/efeitos dos fármacos , Regulação Bacteriana da Expressão Gênica/genética , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Regulação da Expressão Gênica no Desenvolvimento/genética , Mucosa Intestinal/microbiologia , Fígado/microbiologia , Linfonodos/microbiologia , Oligossacarídeos/administração & dosagem , Probióticos/administração & dosagem , Distribuição Aleatória , Suínos , Receptor 2 Toll-Like/biossíntese , Receptor 2 Toll-Like/genética , Receptor 4 Toll-Like/biossíntese , Receptor 4 Toll-Like/genética , Receptores Toll-Like/biossíntese , Fator de Necrose Tumoral alfa/metabolismo , DesmameRESUMO
During the last decades, the number of vehicles per citizen as well as the traffic speed and load has dramatically increased. This sudden and somehow unplanned overloading has strongly shortened the life of pavements and increased its cost of maintenance and risks to users. In order to limit the deterioration of road networks, it is necessary to improve the quality and performance of pavements, which was achieved through the addition of a polymer to the bituminous binder. Since their introduction, polymer-modified asphalts have gained in importance during the second half of the twentieth century, and they now play a fundamental role in the field of road paving. With high-temperature and high-shear mixing with asphalt, the polymer incorporates asphalt molecules, thereby forming a swallowed network that involves the entire binder and results in a significant improvement of the viscoelastic properties in comparison with those of the unmodified binder. Such a process encounters the well-known difficulties related to the poor solubility of polymers, which limits the number of macromolecules able to not only form such a structure but also maintain it during high-temperature storage in static conditions, which may be necessary before laying the binder. Therefore, polymer-modified asphalts have been the subject of numerous studies aimed to understand and optimize their structure and storage stability, which gradually attracted polymer scientists into this field that was initially explored by civil engineers. The analytical techniques of polymer science have been applied to polymer-modified asphalts, which resulted in a good understanding of their internal structure. Nevertheless, the complexity and variability of asphalt composition rendered it nearly impossible to generalize the results and univocally predict the properties of a given polymer/asphalt pair. The aim of this paper is to review these aspects of polymer-modified asphalts. Together with a brief description of the specification and techniques proposed to quantify the storage stability, state-of-the-art knowledge about the internal structure and morphology of polymer-modified asphalts is presented. Moreover, the chemical, physical, and processing solutions suggested in the scientific and patent literature to improve storage stability are extensively discussed, with particular attention to an emerging class of asphalt binders in which the technologies of polymer-modified asphalts and polymer nanocomposites are combined. These polymer-modified asphalt nanocomposites have been introduced less than ten years ago and still do not meet the requirements of industrial practice, but they may constitute a solution for both the performance and storage requirements.
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Baby formula acidification can be used to reduce diarrhea. Calcium formate is a dietary acidifier frequently used in animal weaning diets; it is also a source of available calcium. Gastric acidification reduces gastrin release and hydrochloric acid (HCl) secretion. To study the medium-term effects on fundic gastric mucosa, we fed weaning pigs control diets or diets supplemented with free or fat-protected calcium formate. We evaluated the following: 1) the number of HCl-secreting parietal cells, by immunohistochemistry using an antibody against H(+)/K(+)-ATPase; 2) the number of enteroendocrine cells immunohistochemically stained with chromogranin A (CGA), somatostatin, and histamine (HIS); and 3) the expression of the H(+)/K(+)-ATPase gene, by real-time RT-PCR in the oxyntic mucosa. Cells co-staining for CGA and HIS were defined as enterochromaffin-like (ECL) cells. Pigs fed calcium formate had fewer parietal cells and a lower expression of the H(+)/K(+)-ATPase gene than the controls (P < 0.05). This reduction did not occur in pigs fed fat-protected calcium formate. Somatostatin immune-reactive cells were also more numerous in pigs fed free calcium formate than in controls (P < 0.05). The number of ECL cells was not affected. Using covariance analysis, the number of parietal cells explained part of the differences in the expression of H(+)/K(+)-ATPase gene (positive correlation, r = 0.385, P < 0.01), and excluded the statistical significance of the diet. In the future, the effects on the oxyntic mucosa should be checked when the diet supplemented with calcium formate is discontinued. Furthermore, a reduction in the number of parietal cells could impair the absorption of vitamin B-12 due to a reduced secretion of the intrinsic factor by these cells.