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1.
Orthod Craniofac Res ; 20(3): 164-169, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28653350

RESUMO

OBJECTIVE: To evaluate orthodontic tooth movement (OTM) in rats treated with two types of bisphosphonates (BPs), alendronate sodium (A) and zoledronic acid (Z). DESIGN: In all, 15 male Wistar rats were randomly divided into three groups. Group OTM+A: orthodontic tooth movement and subcutaneous administration of alendronate sodium (2.5 mg/kg); Group OTM+Z: orthodontic tooth movement and subcutaneous administration of zoledronic acid (0.02 mg/kg), and Group OTM: orthodontic tooth movement and subcutaneous injection of saline. The BPs were administered once a day during 25 days before OTM started and during 10 days of OTM. The left upper first molar was moved with a stainless-steel closed coil spring which delivered an initial force of 0.4N. OTM was measured with a digital caliper comparing the moved and the contralateral side. The histomorphometric analysis counted the number of osteoclasts, inflammatory cells, blood vessels and fibroblasts (n/104  m2 ) in periodontal ligament (PDL) of the distobuccal root. RESULTS: A reduction of 58.3% of OTM was found in Group OTM+A and 99.6% in Group OTM+Z, when compared with Group OTM. There was a significant decrease of osteoclasts and inflammatory cells in BP-treated groups. Blood vessels and fibroblastic cells decreased mainly in Group OTM+Z. CONCLUSION: Alendronate sodium and zoledronic acid have similar effects on the periodontal tissue during orthodontic treatment in rats. Especially, zoledronic acid can affect orthodontic tooth movement.


Assuntos
Alendronato/farmacologia , Remodelação Óssea/efeitos dos fármacos , Difosfonatos/farmacologia , Imidazóis/farmacologia , Técnicas de Movimentação Dentária , Animais , Densidade Óssea/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar , Ácido Zoledrônico
2.
Colloids Surf B Biointerfaces ; 66(2): 253-9, 2008 Oct 15.
Artigo em Inglês | MEDLINE | ID: mdl-18676122

RESUMO

In this work the structural features of microemulsions (MEs) containing the pharmaceutical biocompatible Soya phosphatidylcholine/Tween 20 (1:1) as surfactant (S), Captex 200 as oil phase (O), and phosphate buffer 10mM, pH 7.2 as aqueous phase (W) were studied. Systems obtained with different proportions of the components were described by pseudo-ternary phase diagrams in order to characterize the microemulsions studied here. MEs were prepared with and without the polyene antifungal drug amphotericin B (AmB). The maximum AmB incorporation into the ME system was dependent on both the oil phase and surfactant proportions with 6.80 and 5.7 mg/mL in high contents, respectively. The incorporation of AmB into the ME systems significantly increased the profile of the droplet size of the ME for all ranges of surfactant proportions used in the formulations. The microstructures of the system were characterized by dynamic light scattering (DLS) and rheological behavior. The DLS results showed that the size of the oil droplets increases 4.6-fold when AmB is incorporated into the ME system. In all cases the increase in the proportion of the oil phase of the ME leads to a slight increase in the diameter of the oil droplets of the system. Furthermore, for both the AmB-loaded and AmB-unloaded MEs, the size of the oil droplets decrease significantly with the increase of the S proportion in the formulations, demonstrating the efficiency of the surfactant in stabilizing the ME. Depending on the ME composition, an anti-thixotropic behavior was found. The maximum increases of the consistency index caused by the increase of the oil phase of the ME were of 17- and 25-times for the drug-loaded and drug-unloaded MEs, respectively. However, the observed effect for the drug-loaded ME was about 4.6 times higher than that for the drug-unloaded one, demonstrating the strong effect of the drug on the rheological characteristics of the ME system. Therefore, it is possible to conclude that the investigated ME can be used as a very promising vehicle for AmB.


Assuntos
Anfotericina B/química , Caprilatos/química , Sistemas de Liberação de Medicamentos , Lecitinas/química , Óleos/química , Polietilenoglicóis/química , Emulsões , Luz , Conformação Molecular , Tamanho da Partícula , Fosfatos/química , Fosfatidilcolinas/química , Polissorbatos/química , Espalhamento de Radiação , Glycine max/química , Propriedades de Superfície , Tensoativos/química , Água/química
3.
Colloids Surf B Biointerfaces ; 63(2): 287-95, 2008 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-18313903

RESUMO

Depending on the composition, the mixture of surfactant, oil and water, may form supramolecular aggregates with different structures which can significantly influence the drug release. In this work several microemulsion (ME) systems containing soya phosphatidylcholine (SPC) and eumulgin HRE40 (EU) as surfactant, cholesterol (O) as oil phase, and ultra-pure water as an aqueous phase were studied. MEs with and without the antitumoral drug doxorubicin (DOX) were prepared. The microstructures of the systems were characterized by photon correlation spectroscopy, rheological behavior, polarized light microscopy, small-angle X-ray scattering (SAXS) and X-ray diffraction (XRD). The results reveal that the diameter of the oil droplets was dependent on the surfactant (S) amount added to formulations. The apparent viscosity was dependent on the O/S ratio. High O/S ratio leads to the crystallization of cholesterol polymorphs phases which restricts the mobility of the DOX molecules into the ME structure. Droplets with short-range spatial correlation were formed from the ME with the low O/S ratio. The increase of the cholesterol fraction in the O/S mixture leads to the formation of ordered structures with lamellar arrangements. These different structural organizations directly influenced the drug release profiles. The in vitro release assay showed that the increase of the O/S ratio in the formulations inhibited the constant rate of DOX release. Since the DOX release ratio was directly dependent on the ratio of O/S following an exponential decay profile, this feature can be used to control the DOX release from the ME formulations.


Assuntos
Materiais Biocompatíveis , Doxorrubicina/química , Emulsões , Glycine max/química , Fosfatidilcolinas/química , Viscosidade , Difração de Raios X
4.
J Sports Med Phys Fitness ; 45(4): 529-31, 2005 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16446686

RESUMO

AIM: Brain natriuretic peptide (BNP) is a cardioactive molecule produced in the myocardium. BNP is a sensitive marker of cardiac failure and its measurement in blood could be useful to the diagnosis and the treatment of this disease. Sporting activities, especially endurance ones, can induce cardiac problems, owing to the high workload for the myocardium during long and ultralong heavy effort. There are 2 papers describing the behavior of BNP in endurance events. BNP was elevated in marathoners, immediately after the race and also after 4 h. We studied the behavior of BNP in the triathlon, which is a complex sport characterized by 3 different activities (swimming, cycling, running). METHODS: We recruited 49 athletes, all males, except for 4 females; 2 athletes did not finish the race and were not included in the statistical analysis in 2 different competitions. In these subjects we measured BNP using an immunological method before and after a triathlon. RESULTS: No statistical significance between BNP values, before and after the triathlon, was found. CONCLUSIONS: We found no significant differences between pre- and postcompetition BNP values. Moreover, the range of values in both the blood drawings are similar of those of the general population, representing the biological variability of the analyte. The values in regularly trained athletes,, are not different from the general population and BNP is not modified by a triathlon, a typical endurance sport performance. We can underline that BNP increases in plasma are induced by heavy pathologies and are not influenced by physical activities, even strenuous ones.


Assuntos
Peptídeo Natriurético Encefálico/sangue , Resistência Física/fisiologia , Esportes/fisiologia , Adulto , Ciclismo/fisiologia , Biomarcadores/sangue , Cardiomiopatias/diagnóstico , Cardiomiopatias/fisiopatologia , Comportamento Competitivo/fisiologia , Exercício Físico/fisiologia , Feminino , Humanos , Masculino , Estudos Prospectivos , Corrida/fisiologia , Natação/fisiologia , Fatores de Tempo
5.
J Cereb Blood Flow Metab ; 18(6): 639-45, 1998 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-9626188

RESUMO

Little is known of the factors that regulate CBF in sleep. We therefore studied 10 lambs to assess the vasodilatory processes that underlie cerebral autoregulation during sleep. Lambs, instrumented to measure CBF (flow probe on the superior sagittal sinus), sleep state, and cerebral perfusion pressure (CPP), were rapidly made hypotensive by inflating a cuff around the brachiocephalic artery to reduce CPP to 30 mm Hg in each state. During control periods, cerebral vascular resistance (CVR in mm Hg/mL/min) was lower in active sleep (2.8 +/- 0.3, mean +/- SD, P < or = 0.001) than in wakefulness (3.9 +/- 0.6) and quiet sleep (4.3 +/- 0.6). The CVR decreased promptly in each state as CPP was lowered. The time (seconds) required for maximal cerebral vasodilation to occur was longer in active sleep (35 +/- 11) than in quiet sleep (20 +/- 6, P < or = 0.001) and wakefulness (27 +/- 11, P < or = 0.05). The CVR decreased less in active sleep (0.6 +/- 0.3, P < or = 0.001) than in quiet sleep (1.5 +/- 0.3), although the changes in CPP induced with brachiocephalic occlusion were equal in each state. In conclusion, our studies provide the first evidence that the vasoactive mechanisms that underlie autoregulation of the cerebral circulation function during sleep. Moreover, our data reveal that the speed and the magnitude of the vasodilatory reserves available for autoregulation are significantly less in active sleep than in quiet sleep.


Assuntos
Animais Recém-Nascidos , Encéfalo/irrigação sanguínea , Circulação Cerebrovascular , Hipotensão , Sono/fisiologia , Vasodilatação , Animais , Pressão Sanguínea , Artérias Cerebrais , Constrição , Homeostase , Ovinos , Resistência Vascular
6.
J Cereb Blood Flow Metab ; 16(6): 1312-8, 1996 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-8898706

RESUMO

Brain capillary perfusion was evaluated in the different states of the wake-sleep cycle-quiet wakefulness (QW), quiet sleep (QS), and active sleep (AS)-in rats. The extent of the perfused capillary network was determined by intravascular distribution of a fluorescent marker. Evans blue (EB); it remained unchanged across the three behavioral conditions, QW, QS, and AS. The anatomical network was assessed by alkaline phosphatase (AP) endothelial staining, which is known to underestimate the number of existing capillaries. The resulting number of AP profiles were, therefore, significantly lower than the number of EB profiles, but the percentage of AP-stained capillaries that were perfused (96%) was also unchanged across the behavioral conditions. The results indicate that no capillary recruitment accompanies the wake-sleep cycle. Capillary surface area is a relevant factor in determining exchanges across the blood-brain barrier. In the absence of capillary recruitment (relative constancy of the surface area), the CBF changes during sleep should preferentially affect flow-limited with respect to diffusion-limited transport.


Assuntos
Encéfalo/irrigação sanguínea , Capilares/fisiologia , Sono/fisiologia , Animais , Encéfalo/fisiologia , Capilares/anatomia & histologia , Azul Evans , Corantes Fluorescentes , Masculino , Perfusão , Ratos , Ratos Sprague-Dawley
7.
Sleep ; 24(7): 753-8, 2001 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-11683478

RESUMO

UNLABELLED: Spontaneous fluctuations in Heart Period (HP) and Mean Arterial Pressure (MAP) make it possible to evaluate baroreceptor-heart rate reflex sensitivity (BRS). 30-s sequences of HP and MAP beat-to-beat values were considered in the different wake-sleep states (Wake, W; Quiet Sleep, QS; Active Sleep, AS) in rats to assess whether 1) BRS changes between states and 2) the different indexes supply consistent BRS measures. BRS indexes were calculated according to validated literature procedures as regression coefficients of HP vs. MAP 1) within all ramps of increasing or decreasing MAP of four beats or more, with HP and MAP changing in the same direction (baroreflex-mediated fluctuations, BRSp), 2) within all such ramps irrespective of the relative direction of HP and MAP changes (baroreflex + non-baroreflex, i.e. non-homeostatic centrally driven, fluctuations, BRSA). HP vs. MAP regression coefficient along the entire 30-s sequence (bHPMAP) was also calculated. RESULTS: BRSp did not change among states, BRSA decreased from QS to W to AS, bHPMAP decreased from QS to W and became negative in AS. CONCLUSIONS: 1) as indicated by BRSp, baroreflex sensitivity is state independent, 2) BRSp to BRS(A) to bHPMAP are increasingly affected by non-baroreflex fluctuations, BRSp being most apt to measure BRS, 3) non-homeostatic MAP and HP fluctuations increase from QS to W and prevail in AS. These potentially harmful fluctuations are normally buffered by baroreflexes: in the case of baroreflex impairment, circulatory risk may arise in conditions like AS, when they prevail.


Assuntos
Vias Auditivas/fisiologia , Barorreflexo/fisiologia , Frequência Cardíaca/fisiologia , Ratos/fisiologia , Sono/fisiologia , Vigília/fisiologia , Animais , Pressão Sanguínea/fisiologia , Eletroencefalografia , Eletromiografia , Masculino , Ratos Sprague-Dawley
8.
Brain Res ; 111(2): 253-60, 1976 Jul 30.
Artigo em Inglês | MEDLINE | ID: mdl-949599

RESUMO

The effects on respiratory frequency of preoptic heating during slow-and fast-wave sleep were studied in freely moving cats. During slow-wave sleep, at neutral ambient temperature, the subliminal range of preoptic temperature for thermal polypnea extends to 0.2-0.4 degrees C above control preoptic temperature. During fast-wave sleep, at neutral ambient temperature, the subliminal range of preoptic temperature for thermal polypnea increases beyond 0.9-1.0 degrees C above control preoptic temperature. Moreover, the polypneic response is weak and unstable. The results show the suspension of a precise thermoregulatory control during fast-wave sleep in a homoiothermic species.


Assuntos
Regulação da Temperatura Corporal , Hipotálamo/fisiologia , Área Pré-Óptica/fisiologia , Respiração , Sono/fisiologia , Animais , Temperatura Corporal , Mapeamento Encefálico , Gatos , Fases do Sono/fisiologia
9.
Brain Res ; 641(1): 46-50, 1994 Mar 28.
Artigo em Inglês | MEDLINE | ID: mdl-8019850

RESUMO

Cerebral blood flow (CBF) and blood flow (BF) in extracerebral head structures were measured during the sleep-wake cycle in rats using radioactive microspheres. While no statistically significant changes occurred in the transition from Waking to quiet sleep (also referred to as synchronized or non-REM Sleep), CBF increased significantly in active sleep (AS, also referred to as desynchronized or REM Sleep) in all structures considered, with the sole exception of the cerebellum. In extracerebral head structures, no significant state-dependent BF changes were found. Factor Analysis however extracted a common factor accounting for BF variability in the external carotid circulation. This factor was uncorrelated with CBF changes in AS, suggesting independent regulation of the two vascular beds in this sleep state.


Assuntos
Artéria Carótida Primitiva/fisiologia , Circulação Cerebrovascular/fisiologia , Sono/fisiologia , Animais , Masculino , Ratos , Ratos Sprague-Dawley , Fluxo Sanguíneo Regional/fisiologia
10.
Brain Res ; 415(1): 14-20, 1987 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-3620941

RESUMO

Regional cerebral blood flow (rCBF) was measured in rabbits during the sleep-waking cycle, using radioactive microspheres. rCBF does not change significantly in the transition from wakefulness (W) to synchronized sleep (SS), while it significantly increases during desynchronized sleep (DS). No correlation was found between CBF changes and changes in cardiac output or regional blood flows in the transition from SS to DS. This suggests that the CBF increase in DS is not relevantly affected by changes in extracerebral hemodynamic factors.


Assuntos
Encéfalo/fisiologia , Circulação Cerebrovascular , Hemodinâmica , Sono/fisiologia , Animais , Pressão Sanguínea , Frequência Cardíaca , Microesferas , Coelhos , Vigília/fisiologia
11.
Clin Biochem ; 31(6): 449-57, 1998 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9740966

RESUMO

The analytical accuracy of the results of routine clinical chemistry measurements is contributed by a two-steps mechanism, involving transferring trueness from a higher metrological and monitoring the time-stability of trueness itself. In both operations, different materials are used: however, accuracy in the routine assay of genuine patient samples has to be the end product of this overall process. To such an aim, the materials must show an intermethod behavior similar to that of patient sera, i.e., they have to show commutability. Definitions of commutability and methods for assessing such a property are mentioned. The following aspects of lack of commutability of materials are then discussed: frequency; effects on the measured interlaboratory variability; and effects on the recalibration of analytical systems. The causes giving rise to lack of commutability are neither clear or easy to be shown. Matrix effect is one of the main causes; also, differences in the characteristics of the component being measured are often responsible for noncommutability of materials for enzyme activity measurements. Examples of these two different situations are given. It is concluded that, for an efficacious overall quality assurance process, either a set of minimally processed patient sera or commutable reference materials are to be used in the operations concerned with the control of trueness. An additional alternative approach is based on the use of materials with system-specific assigned values.


Assuntos
Técnicas de Laboratório Clínico/normas , Animais , Humanos , Padrões de Referência , Valores de Referência
12.
Neurosci Lett ; 163(2): 173-6, 1993 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-8309626

RESUMO

Regional spinal cord blood flow was measured in rats during the sleep-wake cycle with the use of radioactive microspheres. Spinal cord blood flow decreases from wakefulness to quiet (synchronized) sleep while increasing in active (desynchronized) sleep. Blood-flow changes depend on changes in vascular resistance whose mechanisms have yet to be elucidated. Blood-gas tension or mean arterial pressure, however, do not play a relevant causal role.


Assuntos
Sono/fisiologia , Medula Espinal/irrigação sanguínea , Vigília/fisiologia , Animais , Pressão Sanguínea , Radioisótopos de Cobalto , Eletroencefalografia , Eletromiografia , Frequência Cardíaca , Masculino , Microesferas , Radioisótopos , Ratos , Ratos Sprague-Dawley , Fluxo Sanguíneo Regional , Medula Espinal/fisiologia , Estrôncio , Estanho , Resistência Vascular
13.
Clin Chim Acta ; 135(2): 175-9, 1983 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-6652926

RESUMO

Human blood haemolysates containing ethylene glycol (final volume fraction 0.35) were prepared and stored at -20 degrees C (in the liquid state) up to 372 days. During the whole period, the total haemoglobin concentration (assayed in the material by means of the reference HiCN method) was found to be stable; spectral analysis also failed in detecting any deterioration, Hi formation being low. Good stability was also recorded on storage at 2-4 degrees C for 15 days, but only for 1-2 days at room temperature. The stabilised haemolysate is suggested as a material for long-term control of accuracy in hemoglobinometry.


Assuntos
Etilenoglicóis , Hemoglobinometria/métodos , Etilenoglicol , Hemoglobinas/análise , Humanos
14.
Clin Chim Acta ; 69(1): 73-9, 1976 May 17.
Artigo em Inglês | MEDLINE | ID: mdl-1269155

RESUMO

From a concentrated solution of bilirubin in dimethylsulfoxide (about 13 mmol/1, standardized spectrophotometrically) and a serum diluted 1/3 (whose native bilirubin is lowered by exposure to phototherapy lamps) a bilirubin-enriched serum is prepared (bilirubin concentration about 130 mumol/1 = 7.5 mg/dl) wherein all the bilrubin is bound to albumin. Such an enriched serum behaves in the diazo-reaction in the same way as a bilirubin-enriched serum prepared with undiluted serum and can, therefore, be used as a working standard in serum bilirubin assays.


Assuntos
Bilirrubina/sangue , Compostos de Diazônio , Dimetil Sulfóxido , Estudos de Avaliação como Assunto , Humanos , Indicadores e Reagentes , Ligação Proteica , Albumina Sérica , Espectrofotometria/métodos
15.
Clin Chim Acta ; 298(1-2): 181-6, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10876014

RESUMO

Serum transferrin saturation (TS) values were calculated on the basis of serum iron and transferrin (protein) measurements in a total of 2425 serum samples from six groups of subjects: individuals applying for selection as blood donors (M and F, median age 34 and 32 years); patients referring to the hospital laboratory for routine testing (M and F, median age 45 and 48 years); and elderly subjects living in a specialized institute (M and F, median age 76 and 82 years). In the first four groups the frequency of TS values <15% and >62% respectively, was substantially as expected, considering the average health conditions and sex. These results indirectly support the reliability of the measurement procedure. In the elderly group, however, the frequency of TS values >62% was zero. Mean TS values in the elderly group (males and females) were significantly lower (P<0. 0001) than in the blood donors group and in the hospital patients one. This observation suggests a shortened survival in the presence of (unrecognized) iron overload, pointing out at the usefulness of iron overload screening using simple biochemical tests.


Assuntos
Envelhecimento/sangue , Transferrina/metabolismo , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Ferro/sangue , Deficiências de Ferro , Sobrecarga de Ferro/sangue , Masculino , Pessoa de Meia-Idade , Peso Molecular , Valores de Referência , Transferrina/análise
16.
Clin Chim Acta ; 303(1-2): 55-60, 2001 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-11163023

RESUMO

The low biological variation of myoglobin and creatine kinase isoenzyme MB mass (CK-MBm) requires accurate measurements. In the standardization process, in order to effectively measure and correct intermethod variability, the intermethod behaviour of control materials must be the same of patient sera, i.e. they must be commutable. In this work we checked the commutability of some commercially available control materials in pairs of methods for myoglobin and CK-MBm measurements; we assessed the impact of commutable and non-commutable control materials when used for equalizing patient sera results by two different methods and discussed the problems related to external quality assessment schemes. Myoglobin and CK-MBm were measured in sets of 49 and 56 patient sera and in 13 commercially available control materials with two automatic analytical systems. The non-commutability rate was 8.3% for myoglobin and 23.1% for CK-MBm. Recalculation of serum samples results with a control material as calibrator lowered or increased the bias originally present according to whether the material itself was commutable or not. We conclude that also for myoglobin and CK-MBm assays it is necessary to check the commutability of materials to be used in external quality assessment schemes, or to normalize patient results by different methods.


Assuntos
Creatina Quinase/sangue , Isoenzimas/sangue , Mioglobina/sangue , Creatina Quinase Forma MB , Humanos , Imunoensaio/métodos , Padrões de Referência , Reprodutibilidade dos Testes
17.
Dig Liver Dis ; 36(2): 135-40, 2004 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-15002822

RESUMO

BACKGROUND: Authors generally agree that Giant Pancreatic Pseudocysts (> 10 cm) have a lower spontaneous resolution and are more difficult to treat than smaller pancreatic pseudocysts. This study was carried out on two groups of patients with larger and smaller pancreatic pseudocysts (pancreatic pseudocysts > 10 cm versus pancreatic pseudocysts < 10 cm), and aims to establish whether the size of pancreatic pseudocysts is a factor influencing treatment outcomes. PATIENTS AND METHODS: In a retrospective study, we examined 71 patients with pancreatic pseudocysts following an episode of acute pancreatitis, which were treated in our hospital from 1980 to 2000. Forty-one (57.5%) patients had a large pancreatic pseudocyst. Most patients underwent invasive treatments: 9 (12.6%) had percutaneous drainage, 37 (52.1%) open surgery and 13 (18.3%) endoscopic cyst gastrostomy. 12 patients (16.9%) of the 71 were cured with medical therapy alone. RESULTS: As far as the aetiology of the pancreatitis, location and number of the cysts were concerned, no major differences emerged between the two groups, although large pancreatic pseudocysts followed more severe pancreatitis (P = 0.0005). All giant pancreatic pseudocysts required invasive treatments; 40% of the pancreatic pseudocysts < 10 cm were successfully treated with medical therapy alone. No statistical differences were found regarding hospital mortality, morbidity, recurrence rate and hospital stay among the patients treated invasively. CONCLUSIONS: Giant pancreatic pseudocysts more often require invasive therapy due to persistent symptoms or complications. Treatment outcomes do not seem to be influenced by the size of the pancreatic pseudocysts.


Assuntos
Pseudocisto Pancreático/fisiopatologia , Pseudocisto Pancreático/terapia , Procedimentos Cirúrgicos Operatórios/métodos , Adulto , Idoso , Pesos e Medidas Corporais , Endoscopia do Sistema Digestório/métodos , Feminino , Humanos , Tempo de Internação , Masculino , Pessoa de Meia-Idade , Recidiva , Estudos Retrospectivos , Resultado do Tratamento
18.
Ann Clin Biochem ; 22 ( Pt 3): 257-60, 1985 May.
Artigo em Inglês | MEDLINE | ID: mdl-2411186

RESUMO

Stabilised haemolysates, to be stored at -20 degrees C in the liquid state, have been evaluated in an interlaboratory trial: haemoglobin A2 and haemoglobin F levels were stable for at least 1 year. Since the materials can be very easily prepared in each laboratory, they appear to meet the requirements for use in internal quality-control schemes, for the assay of both haemoglobin fractions.


Assuntos
Hemoglobina Fetal/análise , Hemoglobina A2/análise , Hemoglobina A/análise , Estabilidade de Medicamentos , Estudos de Avaliação como Assunto , Humanos , Controle de Qualidade , Padrões de Referência , Fatores de Tempo
19.
J Int Med Res ; 20(2): 136-49, 1992 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-1521670

RESUMO

A study was carried out in 73 male or female patients suffering from mild vascular dementia. The experimental protocol involved an initial run-in period (14 days) and subsequent double-blind, randomized treatment with 300 mg buflomedil or placebo given orally twice daily for 90 days. At the end of this treatment period, all patients received buflomedil for a further 90 days and, thereafter, patients received buflomedil or no further treatment for another 90 days. The efficacy of buflomedil was monitored using rating scales and neuropsychological tests. The findings of the study indicate that buflomedil improved the symptoms of vascular dementia; the most improvement was recorded in patients who had received buflomedil for the longest period. Inadequacy, cooperation, psycho-affective disorders, self-care and somatic complaints were positively influenced by buflomedil. The efficacy of buflomedil may be due to its effects on platelet aggregation and improvement in blood flow distribution to the ischaemic brain and oxygenation of brain tissue.


Assuntos
Demência Vascular/tratamento farmacológico , Pirrolidinas/uso terapêutico , Vasodilatadores/uso terapêutico , Idoso , Demência Vascular/fisiopatologia , Demência Vascular/psicologia , Feminino , Humanos , Masculino , Testes Neuropsicológicos , Pirrolidinas/efeitos adversos , Fatores Socioeconômicos
20.
Minerva Med ; 83(6): 389-93, 1992 Jun.
Artigo em Italiano | MEDLINE | ID: mdl-1630702

RESUMO

Approximately one week after a cycle of antibiotic therapy using ampicillin for pulmonary inflammation, an 82-year-old woman developed symptoms of acute renal insufficiency reminiscent of nephrotic syndrome. Symptoms were completely reversible and normal renal function was recovered (even after 14 months of follow-up). The Authors conclude by attributing this to acute tubulo-interstitial nephropathy due to hypersensitivity to ampicillin.


Assuntos
Injúria Renal Aguda/induzido quimicamente , Ampicilina/efeitos adversos , Hipersensibilidade a Drogas/etiologia , Idoso , Idoso de 80 Anos ou mais , Feminino , Seguimentos , Humanos , Testes de Função Renal , Síndrome Nefrótica/induzido quimicamente , Fatores de Tempo
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