RESUMO
Hypothyroidism is the most commonly diagnosed endocrine disease in dogs. The objective of this study was to evaluate the changes in the redox status in canine hypothyroidism using whole blood (WB) and red blood cell (RBCs) lysates. For this purpose, a panel of five antioxidants and five oxidants biomarkers was measured in WB and RBCs lysates of 30 dogs with hypothyroidism, 26 dogs with non-thyroidal illnesses and 15 healthy dogs. The antioxidants measured were cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), Trolox equivalent antioxidant capacity (TEAC), thiol and paraoxonase type-1 (PON-1). Oxidants measured include the total oxidant status (TOS), peroxide-activity (POX-Act), reactive oxygen-derived metabolites (d-ROMs), advanced oxidation protein products (AOPP) and thiobarbituric acid reactive substances (TBARS). WB showed a significant decrease of the antioxidants CUPRAC, TEAC and thiol, and also an increase in TBARS and a decrease in AOPP in dogs with hypothyroidism compared to healthy dogs. Meanwhile, RBCs lysates showed a significant increase in FRAP and PON-1 in dogs with hypothyroidism. The changes in the redox biomarkers in this study show that WB in canine hypothyroidism had a higher number of changes in biomarkers of the redox status than RBCs lysates, making it a promising sample type for the evaluation of the redox status in this disease. In addition, WB is easier and simpler to process than RBCs lysates and unlike serum, it does not have any hemolysis interference.
Assuntos
Biomarcadores , Doenças do Cão , Eritrócitos , Hipotireoidismo , Oxirredução , Animais , Cães , Hipotireoidismo/veterinária , Hipotireoidismo/sangue , Doenças do Cão/sangue , Biomarcadores/sangue , Eritrócitos/metabolismo , Masculino , Feminino , Antioxidantes/metabolismo , Estresse Oxidativo , Estudos de Casos e ControlesRESUMO
Objetivo Analizar las diferencias clínicas y de tratamiento entre los cánceres de mama diagnosticados en el programa de cribado en la provincia de Segovia durante los años 1992-2007, y los diagnosticados en el mismo periodo de tiempo en el dispositivo asistencial habitual del Hospital General de Segovia. Material y métodos Estudio retrospectivo descriptivo, analiza variables tipo histológico, tamaño tumoral, ganglios afectos, estadio, hormonodependencia, grado de diferenciación, tipos de tratamiento y evolución clínica en ambas muestras. Se utilizaron tablas de contingencia para evaluar la posible existencia de asociación entre las variables clínicas. Resultados Se detectó en el grupo diagnosticado por el cribado un mayor porcentaje de carcinomas in situ (15,4 vs. 7,1%), menor tasa de infiltración ganglionar (45 vs. 70% consulta), tratamientos menos agresivos (el 30,1% recibe quimioterapia grupo cribado vs. 50,3% programa) y una tasa menor de fallecimientos (5,1% programa vs. 23% consulta). Conclusiones Este estudio muestra que hay diferencias en cuanto al comportamiento biológico de los tumores y en los protocolos de tratamiento aplicados según el proceso de diagnóstico se inicie a través del cribado o en el marco asistencial (AU)
Objective To analyze differences in clinical variables and treatment between breast cancer diagnosed in the screening program of the Spanish province of Segovia and breast tumors diagnosed in the same period in routine clinical practice. Materials and methods A descriptive and retrospective study was conduced to analyze the histological variables, tumor size, lymph node involvement, stage, hormone status, degree of differentiation, types of treatment and clinical outcome in both groups. Contingency tables were used to evaluate the association among clinical variables. Results In the group diagnosed in the screening program, there was a greater proportion of carcinomas in situ (15.4 vs 7.1%), a lower rate of lymph node infiltration (45 vs 70%), less aggressive treatment (30.1 vs 50.3% receiving chemotherapy), and lower mortality (5 vs 23%).ConclusionsT his study shows that there are differences in the biological behavior of tumors and treatment protocols applied according to whether the diagnostic process is initiated through a screening program or in routine clinical practice (AU)