Forensic Geochemistry Reveals International Ship Dumping as a Source of New Oil Spill in Brazil's Coastline (Bahia) in Late 2023.

In the present study, we applied forensic geochemistry to investigate the origin and fate of spilled oils like tarballs stranded at the beaches of Bahia, in northeastern Brazil, in September 2023, based on their fingerprints. Saturated and aromatic compounds were assessed by gas chromatography, and the oceanic surface circulation patterns were deciphered to determine the geographic origin of the spill. Contamination by petroleum represents an enormous threat to the unique, species-rich ecosystems of the study area. The geochemical fingerprint of the oil spilled in 2023 did not correlate with those of previous events, including the one in 2019, the one in early 2022 in Ceará, and an extensive spill across the Brazilian Northeast in late 2022. However, the fingerprint did correlate with crude oils produced by Middle Eastern countries, most likely Kuwait. The oil of the 2023 spill had a carbonate marine origin from early mature source rocks. These findings, together with the moderate weathering of the 2023 tarballs and the ocean circulation patterns at the time of the event, indicate that the oil was discharged close to the shore of Brazil, to the east or southeast of Salvador, by a tanker on an international route in the South Atlantic.

An overview of vinasse pollution in aquatic ecosystems in Brazil.

We review the negative impacts of vinasse, a byproduct of alcohol distillation, on Brazil's freshwater ecosystems. We found a total of 37 pollution events between the years 1935 and 2023, with this number almost certainly an underestimate due to underreporting and/or unassessed events. Pollution by vinasse occurred both through accidents (e.g., tank failure) and deliberately (i.e., opening of floodgates), although in many cases the causes remain undetermined. All pollution events caused fish kills, with some records reporting negative effects on other organisms as well (i.e., crustaceans and reptiles). Pollution by vinasse, and associated negative effects, was reported for 11 states, with a notable number of cases in São Paulo. Most cases of vinasse pollution and negative impacts on biodiversity were recorded in rivers, followed by streams and reservoirs. Some of the affected river systems harbour threatened freshwater fishes. Hydrological connectivity means that pollution could have propagated along watercourses. Given these consequences of vinasse pollution on biodiversity, ecosystem functioning and services, we recommend a number of remedial actions.

Irrigation dams threaten Brazilian biodiversity.

Brazil is among the main contributors to global biodiversity, which, in turn, provides extensive ecosystem services. Agriculture is an activity that benefits greatly from these ecosystem services, but at the same time is degrading aquatic and terrestrial ecosystems and eroding Brazilian biodiversity. This conflict is growing, as emerging unsustainable legislative proposals that will benefit the agricultural sector are likely to accelerate the decline of biodiversity. One such initiative (Bill 1282/2019) would change Brazil's "Forest Code" (Law 12,651/2012) to facilitate construction of irrigation dams in Permanent Preservation Areas, a category that includes strips (with or without vegetation) along the edges of watercourses. Two other similar bills are advancing through committees in the Chamber of Deputies. Here we provide details of these three bills and discuss their consequences for Brazil's biodiversity if they are approved. Expected negative impacts with changes in the legislation include: increased deforestation; siltation; habitat fragmentation; introduction of non-native species; reduction in the availability of aquatic habitats; and changes in biogeochemical process. These proposals jeopardize biodiversity and may compromise the negotiations for an agreement between Mercosur and the European Union.

Lessons from the invasion front: Integration of research and management of the lionfish invasion in Brazil.

After successful invasions in the Caribbean and Mediterranean, lionfish (Pterois spp.) have recently invaded another important biogeographical region -the Brazilian Province. In this article, we discuss this new invasion, focusing on a roadmap for urgent mitigation of the problem, as well as focused research and management strategies. The invasion in Brazil is already in the consolidation stage, with 352 individuals recorded so far (2020-2023) along 2766 km of coastline. This includes both juveniles and adults, including egg-bearing females, ranging in length from 9.1 to 38.5 cm. Until now, most of the records in the Brazilian coast occurred in the equatorial southwestern Atlantic (99%), mainly on the Amazon mesophotic reefs (15% of the records), northeastern coast of Brazil (45%), and the Fernando de Noronha Archipelago (41%; an UNESCO World Heritage Site with high endemism rate). These records cover a broad depth range (1-110 m depth), twelve protected areas, eight Brazilian states (Amapá, Pará, Maranhão, Piauí, Ceará, Rio Grande do Norte, Paraíba, and Pernambuco) and multiple habitats (i.e., mangrove estuaries, shallow-water and mesophotic reefs, seagrass beds, artificial reefs, and sandbanks), indicating a rapid and successful invasion process in Brazilian waters. In addition, the lack of local knowledge of rare and/or cryptic native species that are potentially vulnerable to lionfish predation raises concerns regarding the potential overlooked ecological impacts. Thus, we call for an urgent integrated approach with multiple stakeholders and solution-based ecological research, real-time inventories, update of environmental and fishery legislation, participatory monitoring supported by citizen science, and a national and unified plan aimed at decreasing the impact of lionfish invasion. The experience acquired by understanding the invasion process in the Caribbean and Mediterranean will help to establish and prioritize goals for Brazil.

-I fish, therefore I monitor: Participatory monitoring to assess inland small-scale fisheries.

Analysis of small-scale inland fisheries (SSIFs) is often highly dispersed and tends not reflect the true magnitude of their contribution to society. This is partly due to the insufficient attention given to this sector by the relevant authorities, in addition to its highly diverse characteristics, with complex patterns of operation in a wide range of systems, often in remote areas. Here, by integrating fishers as participatory fishery monitors, we provide fishery-dependent estimates of yields, the biological attributes of the fish species, and the spatiotemporal dynamics of the fisheries of lakes on the floodplain of the São Francisco basin in northeastern Brazil. As the fishers were willing participates in the monitoring, the results revealed well-structured artisanal fishing activities, with the lake system providing high-profile fish harvests from both monthly and annual perspectives. The spatial distribution of fishing effort reflected the adaptation of the fishers to the flood cycle of the river, in order to maintain high fishery productivity throughout the year. The results also indicate that participatory monitoring can help to overcome knowledge gaps and provide a database that is readily applicable to management needs at both local and regional scales. As Brazil is one few world's nations that no longer have national fishing monitoring program, participatory monitoring represents a low-cost solution for the credible and useful data on small-scale fisheries. It would thus appear to be extremely worthwhile to invest in the empowerment of communities in order to overcome the historic vulnerability of productive sector and the food security of the populations that depend on these fisheries.

Niche availability and habitat affinities of the red porgy Pagrus pagrus (Linnaeus, 1758): An important ecological player on the world's largest rhodolith beds.

The red porgy (Pagrus pagrus) is a carnivore bottom dweller sparid, inhabiting flat sandy bottoms, rhodolith and seagrass beds of the Mediterranean Sea, the Western Atlantic (from Florida to Argentina) and the Eastern Atlantic (from Britain to Gabon). Along its native range, the red porgy is highly targeted by commercial and artisanal fisheries. In the past 40 years, the population decline of the species has been widely reported. In many locations, such as the Brazilian coast, stocks have collapsed. The central portion of the Brazilian coast harbours the largest rhodolith beds in the world and the highest levels of nektonic and benthic biodiversity. Along the rhodolith megahabitat, P. pagrus density is disproportionately higher (by 480%) than that of conspicuous benthic fishes inhabiting the same environment. Despite the ecological and economic importance of such an important species along its native range, little is known regarding its habitat use, niche availability and population responses to global warming. Here we present habitat affinities based on data sampled using baited remote stereo-video systems, and modelled niche availability and global warming populational responses. Our findings reveal that the red porgy is a species highly associated with rhodolith beds along the central portion of the Brazilian coast. The presence of a disproportional density and biomass of the red porgy, compared to other marine fish species, indicates that the species plays a key ecological role as a carnivore, mesoconsumer and prey/predator tolerant species, maintaining essential ecological functions in the habitat. In a global warming scenario, the model predicted populational niche shifts poleward and a severe niche erosion at lower latitudes as expected. Conservation initiatives (implementation of Maine Protected Areas, trawling exclusion zones, mining exclusion zones, fisheries management policies) are urgent to secure future stocks of the red porgy and also preserve the fragile rhodolith beds they inhabit.

BRUVS reveal locally extinct shark and the way for shark monitoring in Brazilian oceanic islands.

Here we present records of sharks obtained using baited remote underwater stereo-video systems (stereo-BRUVS) at two Brazilian oceanic islands. Fourteen of the 60 deployments recorded 19 sharks in Trindade Island. In Saint Peter and Saint Paul Archipelago (SPSPA), two pelagic and two demersal deployments recorded two and one shark, respectively, including the locally extinct Galapagos shark Carcharhinus galapagensis. Stereo-BRUVS should be considered as adjuncts to other non-invasive methods to monitor shark populations.

Molecular evidence of two new species of Eleotris (Gobiiformes: Eleotridae) in the western Atlantic.

Fishes of the genus Eleotris present highly conserved morphology, which may make their recognition difficult. Here, two cryptic Eleotris lineages from five locations along the coast of Brazil were identified using the COI gene and two nuclear fragments. High bootstrap and posteriori values supported those lineages, and the genetic distance of COI varied from 6% between the two lineages to 14.1% from other western Atlantic Eleotris species, such as E. pisonis, E. amblyopsis and E. perniger. The reciprocal monophyly for both types of markers, the divergences between those lineages and the other Eleotris species from the Brazilian coast may, in fact, represent two new cryptic species. The cryptic lineages and currently recognized species were collected in distinct environments, reinforcing the need for further sampling to understand the real distribution of each taxon.

Population structure and allometry of Podocnemis unifilis (Testudines, Podocnemididae) in a protected area upstream Belo Monte dam in Xingu River, Brazil.

Amazon river turtles are increasingly threatened by habitat loss and alteration due to the Brazilian energy policy based on construction of hydroelectric dams, meanwhile, populational studies remain scarce. We described the population structure, and established body allometric relationships of Podocnemis unifilis in the Terra do Meio Ecological Station in the Iriri River, tributary of the Xingu River upstream the Belo Monte dam under construction Turtles were captured by hand net and diving in 2012 and 2013 dry seasons, and 2013 rainy season. A total of 728 males, 296 females and four juveniles were captured. Adult sex ratio was male-biased by 9.15 ♂:1 ♀. Females were significantly larger than males. Mean straight carapace length was 268.9 ± 46.7 mm (165 - 403) for females; and 232.7 ± 24.8 mm (167 - 303) for males. The sexes were morphologically distinct in function of a proportionally larger plastron, and higher carapace, on females. Allometric relationships between straight carapace length and other morphometric traits were strong for males (R2 range = 0.87 - 0.96 and females (R2 range =0.79 - 0.98. Exploitation of P. unifilis in biomass extirpated from the Middle Xingu River may be estimated from body parts found post-consumption by the presented regressions.

Food consumption as an indicator of the conservation of natural resources in riverine communities of the Brazilian Amazon.

The present study analyzed and compared the daily consumption of foods of animal origin in eleven communities of the Lower Amazon, Trombetas and Purus Rivers, representing three different management systems and levels of conservation in the Brazilian Amazon. All food items of animal origin were weighed by at least 10% of the families in the study communities during a week in each period of the flood cycle between 2006 and 2008. Fish was the most important food, and was consumed during six days of the week, with an average rate of 169 kg.person(-1).year(-1). Game was second in importance, with 37 kg.person(-1).year-(1). This yearly rate of fish consumption is one of the highest in the world and is almost double the minimum recommended by the World Health Organization. The dietary patterns reflect both the isolation of the communities from large urban centers and the better preservation of the local environments due to the existence of protected areas. Environmental degradation may thus have effects on the health and food security of local populations. The study emphasizes the need for the implementation of public policies and participative management initiatives.

Microplastics in the Amazon biome: State of the art and future priorities.

Microplastics (MPs) have been identified as a major potential threat to the biota and human health. Despite the exponential increase in MP research worldwide, few studies have focused on the extensive Amazon biome. To assess research priorities, the present study reviewed and summarized the available scientific knowledge on MPs in the Amazon, in addition to analyzing population and waste-management data, to evaluate potential sources of MPs in the hydrographic system. Poor sanitation conditions are a main source of MPs for the vast hydrographic basin, and, consequently, for the adjacent ocean. Secondary MPs predominated, mostly fibers (96% of debris), composed of polyamide (32%). Mean MP concentrations ranged from 0.34 to 38.3 particles.individual-1 in biota, 5 to 476,000 particles.m-3 in water, and 492.5 to 1.30848 × 107 particles.m-3 in sediment, values in close comparison with those found in areas profoundly affected by anthropogenic pollution. MPs were widespread in a range of Amazonian environments and species, and negative effects are probably occurring at various ecological levels. However, limited research, methodological constraints, flaws and the lack of standardization, combined with the continental dimensions of the Amazon, hampers the collection of the fundamental knowledge needed to reliably evaluate the impacts and implement effective mitigation measures. There is an urgent need to expand scientific data available for the region, improving local research infrastructure, and training and deploying local researchers.

Do coral reefs act as sinks for microplastics?

Microplastic (MP) pollution has been detected in coral reefs, raising concerns regarding its global impact. Although they cover a small portion (<1%) of the total area of the world's oceans, coral reefs are geological and biological structures that trap MPs and disproportionately enhance their accumulation. In this review, we attempted to understand how coral reefs act as short- and long-term sinks for MPs. We describe five characteristics that lead to the enrichment of microplastics in coral reefs: 1) adhesion on reef-building corals at distinct depths; 2) ingestion by reef organisms (e.g., suspension feeders, such as sponges, ascidians, and corals), bioconcentration, and formation of short-term (i.e., years to decades) biological sinks for MPs; 3) formation of long-term (i.e., centuries) MP sinks in coral skeletons and unconsolidated subsurface sediments; 4) reduction of sediment resuspension and seafloor turbulent kinetic energy by complex marine forest architecture that reduces bottom shear stress, facilitates the retention, and deposition of small (<0.5 mm) and high-density floating MPs; and 5) diagenesis of Anthropocene sedimentary rocks containing MPs. We estimate that reef processes may remove more than 10% of floating MPs in shallow tropical waters yearly. Statistical results show that microplastic abundance for reef-building corals are higher than values found in reef sediments and especially in seawater. Moreover, pellets, films, foams and mainly fragments and fibers have been found. These field-based data support our hypothesis of sinks in the reef sediments and organisms. We highlight the role of these seascapes in the interception of MPs as traps and sinks in reef sediments, biota, and carbonate frameworks. As coral reefs are prone to MP accumulation and can become pollution hotspots, global initiatives are necessary to conserve these rich ecosystems and prevent rapidly increasing plastic pollution.

Association between PAH and plastic fragments on Brazilian coast beaches: a baseline assessment.

Plastic materials have many destinies on the environment, and one of them is its continuous fragmentation, originating microplastics (MP). MP act as an adsorption surface for organic pollutants like polycyclic aromatic hydrocarbons (PAH). Due to its toxic and harmful properties, 16 PAH are defined as priorities in studies. Several works have evaluated the occurrence of MP and PAH individually in environmental matrices, but just a few have investigated their association. The present study aims to investigate PAH occurrence, sources, and concentration in MP from beach sediments of eight Brazilian coastal states. Sixteen PAH were analyzed by gas chromatography, in which 14 PAH were detected, and PAH total concentration (ΣPAH) ranged from 0.25 to 71.60 ng g-1. In the North and Northeast regions, the low PAH concentrations in MP appear to be related to the intense hydrodynamic processes. Naphthalene levels on the Styrofoam were above the threshold effect level in two samples and near it on one; therefore, effects in the organisms might occur due to these PAH. The Southeast and South regions are the only ones with previous studies relating PAH with MP, and ΣPAH ranged from 0.34 to 2.52 ng g-1. According to the diagnostic ratios, the PAH sources were associated with industrial and port activities, untreated effluent disposal, and urban runoff by pluvial waters. The highest ΣPAH level was found in the Styrofoam samples, suggesting that this MP type might contribute considerable to PAH dispersion on Brazilian coast beaches.

Marine debris provide long-distance pathways for spreading invasive corals.

Anthropogenic marine debris and invasive species are pervasive in the ocean. However, research on the mechanisms and dynamics controlling their distribution in marine systems (e.g.; by floating debris acting as vectors for invasive species) is limited. Applying a numerical modeling approach, we demonstrate that rafting invasive corals (Tubastraea spp.) can be transported over long distances and reach important tropical receptor regions. In <180 days, buoyant debris can cover distances between 264 and 7170 km moving from the Brazilian semiarid coast to the Amazon coast and reaching eight regions in the Wider Caribbean (mainly the Eastern Caribbean and Greater Antilles). Analyzing 48 simulated scenarios (4 years × 3 depths × 4 months), we demonstrate that in ~86 % of the scenarios the particles are stranded in the Caribbean and in ~71 % they end up in the Amazon coast. Our results showed litter floating trajectories at 0-10 m water depth, transported every year to the Caribbean province. However, in August this transport is frequently blocked by the retroflection of the North Brazil Current adjacent to the Amazon River estuarine plume. Our results indicate routes for fast and long-distance transport of litter-rafting invasive species. We hypothesized a high risk of bioinvasion on important marine ecosystems (e.g., coral reefs) likely becoming increasingly threatened by these invasive species and debris. This highlights the imperative need for an ocean governance shift in prevention, control, and eradication, not only focused on local actions to prevent the spread of invasive species but also a broad international action to decrease and mitigate marine debris pollution globally.

Genetic diversity patterns of lionfish in the Southwestern Atlantic Ocean reveal a rapidly expanding stepping-stone bioinvasion process.

In 2020, multiple lionfish (Pterois spp.) records along the equatorial Southwestern (SW) Atlantic revealed a new expansion of these potentially damaging invasive populations, which could impact over 3500 km of Brazilian coastline over the next few years, as well as unique ecosystems and marine protected areas in its path. To assess the taxonomic status, invasion route, and correlation with other centres of distribution, we investigated the genetic diversity patterns of lionfish caught in 2022 at the Amazonia, Northeastern Brazil, and Fernando de Noronha and Rocas Atoll ecoregions, using two molecular markers, the mitochondrial COI and the nuclear S7 RP1. The data indicate that all studied lionfish belong to what is generally accepted as P. volitans, and share the same genetic signature as lionfish present in the Caribbean Sea. The shared haplotypes and alleles indicate that the SW Atlantic invasion derives from an active movement of adult individuals from the Caribbean Sea into the Brazilian coast. The Amazon mesophotic reefs likely served as a stepping-stone to overcome the biogeographical barrier represented by the Amazon-Orinoco River plume. New alleles found for S7 RP1 suggest the onset of local genetic diversification, heightening the environmental risks as this bioinvasion heads towards other South Atlantic ecoregions.

Microplastics and microfibers in the Guajará Bay, Amazon delta: Potential sources and variability.

The role of Amazon on the transport and as a source of microplastics (MPs) to the ocean is uncertain. This study is an assessment on the distribution of MPs and microfibers (MFs) in a portion of the Amazon delta. Guajará bay is a potential source for surrounding waters, since a metropolis is located at the right margin. Surface water samples were collected during the dry and rainy season of 2014/2015 at six stations. MP and MF abundance ranged from 218 to 5529.98 (1565.01 ± 196.94) particles·m-3. Transparent, white and blue particles were frequent. Higher values were detected on the right, urbanized margin of the bay (p = 0.0124). Most of the particles were anthropogenic cellulose fibers (68.8 %). Polyethylene terephthalate (52.9 %) and polyamide (34.4 %) were the dominant polymers. Our results indicate higher MP and MF abundances near to the potential source, the urban nucleus, and related to local hydrodynamic characteristics.

First Molecular Identification of a Goussia Parasite from a New World Invasive Blenny.

PURPOSE: Introduced or invasive fish are susceptible to new parasites but can simultaneously carry infectious parasites from their native range towards new hosts. Screening these parasites is key to address the health of fish populations and spread of diseases. METHODS: In this study, we sequenced a Coccidia parasite, for the first time from the blenny Omobranchus sewalli, introduced in the northern coast of Brazil with an Indo-Pacific origin. RESULTS: Only one individual was infected, its genetic sequence matched (over 99%) with two lineages of undetermined species, belonging to the genus Goussia, sequenced from three marine fish species (Mulloidichthys flavolineatus, Lutjanus kasmira, and Selar crumenophthalmus) in Hawaii. CONCLUSIONS: Phylogenetic analysis suggests considerable differentiation between the Goussia detected and other Goussia spp. sequenced from North Atlantic marine fish, thus we cannot exclude the possibly that this parasite was carried by O. sewalli from its native Indo-Pacific range.

First record of plastic ingestion by a freshwater stingray.

The abundance and dispersion of plastic particles in aquatic ecosystems has become pervasive resulting in the incorporation of these materials into food webs. Here we describe the first record of plastic ingestion by the freshwater white-blotched river stingray Potamotrygon leopoldi (Potamotrygonidae), an endemic and threatened species in the Xingu River, Amazon basin. Potamotrygonidae stingrays inhabit exclusively Neotropical rivers, occupying rocky substrate habitats and feeding mainly on benthic macroinvertebrates. The gastrointestinal tract of 24 stingrays were analyzed, 16 (66.6 %) of which contained plastic particles. In total, 81 plastic particles were recorded and consisted of microplastics (< 5 mm, n = 57) and mesoplastics (5-25 mm, n = 24). The plastic particles found were classified into fibers (64.2 %, n = 52) and fragments (35.8 %, n = 29). The predominant color was blue (33.3 %, n = 27), followed by yellow (18.5 %, n = 15), white (14.8 %, n = 12), black (13.6 %, n = 11), green (6.2 %, n = 5), transparent (4.9 %, n = 4), pink, grey and brown (2.5 %, n = 2, each) and orange (1.2 %, n = 1). No significant correlation was observed between the number of plastic particles and the body size. Eight types of polymers were identified in the plastic particles analyzed using 2D FTIR Imaging. The most frequent polymer was artificial cellulose fiber. This is the first report of plastic ingestion by freshwater elasmobranchs in the world. Plastic waste has become an emerging problem in aquatic ecosystems globally and our results provide an important datapoint for freshwater stingrays in the Neotropics.

The legacy of artisanal gold mining and its impact on fish health from Tapajós Amazonian region: A multi-biomarker approach.

Tapajós Region, is an area with intense historical artisanal and small-scale gold mining. Therefore, the core objective of this study was to evaluate the environmental status of different rivers located in this region, using biomarker endpoints in Serrasalmus rhombeus as a tool. Fish and sediment were collected from two rivers, Tropas and Crepori, affluent of Tapajós River, located inside a Federal Protection Area and in a Reference site. Mercury concentration in sediment and fish were traced, and biomarkers in gills and liver were analyzed. Results showed a clear difference between these two rivers compared to the Reference site. Fish tissues presented biomarker responses according to the site of collection. Catalase (CAT) activity was statistically higher in fish gills from Crepori, confirming the capacity of mercury interference with redox equilibrium. High levels of lipid peroxidation were also noted to contribute greatly in incidence of morphological changes in the liver and gills, suggesting that mercury bioaccumulation during continuous exposure promote biological responses in a cumulative manner, from molecules to tissues. This study also indicates adaptation in fish defense mechanisms given the conditions in the Tropas River, as well as a variation in biomarker responses to that of the Crepori river. In summary, Tapajós affluents presented high mercury levels in fish tissues leading to biomarker responses, demonstrating a hazardous signal of a long history of mercury pollution.