Identification of specific regions in hepatitis C virus core, NS2 and NS5A that genetically interact with p7 and co-ordinate infectious virus production.

The p7 protein of hepatitis C virus (HCV) is a small, integral membrane protein that plays a critical role in virus replication. Recently, we reported two intergenotypic JFH1 chimeric viruses encoding the partial or full-length p7 protein of the HCV-A strain of genotype 1b (GT1b; Virology; 2007; 360:134). In this study, we determined the consensus sequences of the entire polyprotein coding regions of the wild-type JFH1 and the revertant chimeric viruses and identified predominant amino acid substitutions in core (K74M), NS2 (T23N, H99P) and NS5A (D251G). Forward genetic analysis demonstrated that all single mutations restored the infectivity of the defective chimeric genomes suggesting that the infectious virus production involves the association of p7 with specific regions in core, NS2 and NS5A. In addition, it was demonstrated that the NS2 T23N facilitated the generation of infectious intergenotypic chimeric virus encoding p7 from GT6 of HCV.

Prevalence of Hepatitis B virus between Qeshm Island people in 2013-2014, Iran.

Introduction: About 1/ 3 of the world crowd (2 billion) suffers from HBV infection. 15 to 40% of Hepatitis B cases develop into chronic hepatitis, cirrhosis, and hepatocellular carcinoma (HCC). Considering the dangerous complication of the illness and the evidence that the prevalence is different in various areas of the country, this research was directed with the purpose of determining the currency of the Hepatitis B between Qeshm Island crowds. Method: This cross-partial research was directed on 1500 cases. The sampling procedure was the stratify-cluster organization. Later creating the checklist, including the demographic information and risk factors, blood cases were formed. ELISA system evaluated the currency of HBsAg. At the end, the mathematical studies were conducted by applying the mathematical Plans for software of Social Sciences (SPSS) system issue 16.0. The information were investigated by Chi-square and detailed mathematical exams. Result: The overall currency of HBsAg positivity was 1%, 0.8%, and 1.1% between male and female, individually. The middle age of members was 30.07 years old. Virus was more currency in married persons, students, lower than in 15-years-old educated people and persons who had a past of vaccination and transfusion. The currency of Hepatitis B in people who had a past of sex and substance infusion was zero. Finally, the finding of the research showed that none of the investigated factors was associated with the prevalence of HBsAg. Conclusion: It appears that the currency of HBV virus in Qeshm is slightly lower than that of the public.

Prevalence of HIV disease between Qeshm Island people during 2013-2014, Iran.

Introduction: Got safe loss syndrome (HIV) is represented with a variety of disorders of cellular and humoral immune dysfunction caused with personal immunodeficiency disease (HIV) infection. Immune deficiency caused by HIV, leads to opportunistic diseases & certainly the progression of the infections cause the patient's death. That is why we chose to realize this research, to assess the prevalence of HIV among the Qeshm Island inhabitants. Materials and Method: The cross-sectional research did carry on 1500 subjects. The sampling approach is the stratify-cluster compound. Ten head-clusters were randomly selected from each center and individuals are received from within the blocks. Later developing the questionnaire, including demographic the information and danger agents, gore examples are captured from the brachial vein. The currency of HIV-Ab is assessed with the approach of ELISA. Indeed, the actuarial studies are performed with applying the actuarial Plan for public Sciences software (SPSS) program issue 16.0. The information is examined with Chi-square and detailed actuarial trial. Results: The all over the currency of HIV disease is zero. Of the members in the examination, 511 (34.1%) are men and 989 (65.9%) were women. This age of mediocre is 32.6 years. 88% and 12% of the individuals were married and single, respectively. The training plane of maximum cases (66%) was the degree diploma. In terms of location, mostly subjects (75.2%) lived into the village region. 136 (7.9%) had a history of travelling abroad and none of the subjects did not report a history of running away from home. Conclusion: The most of the subjects lived in the rural area and were married women with high school education. Although there were cases that had records of sexually spread illnesses (STD) or tattoos, HIV prevalence was zero. This gives hope to the health of our society Regarding HIV disease.

Prevalence of Hepatitis C infection in Qeshm Island in 2013-2014, Iran.

Introduction: Hepatitis has involved many individuals and has left many complications. Hepatitis C is a type of hepatitis connected with several dilemmas. The purpose of the research is to study the Hepatitis epidemiology C into the Island of Qeshm in 2014. Method: this was an interventional study conducted on 1500 inhabitants of Qeshm Island. Participants were selected by using cluster sampling. Five cc of blood was drawn from each participant in order to test for HCV-Ab with ELIZA technique. Positive samples were referred for PCR to investigate the presence of anti Hepatitis C anti body. Data were entered in SPSS v.16 after sample collection and are examined utilizing detailed census (prevalence, mean, percent and standard deviation) and chi-square. Results: out of 1500 participants, 986 (65.7%) are women and 514 (34.3 %) are men. HCV anti body was seen in four patients (0.3 percent). The outcomes of the research explained that not of the studied factors (age, gender, marital status, place of residence, educational level, history of IV drug abuse, being in jail, quitting addiction, risky sexual behavior, etc.) is related to antibody pervasiveness. Conclusion: The disease pervasiveness was 0.3 percent in Qeshm Island, that is compatible with the another research outcomes. Also, factors investigated for HCV were not recognized as HCV risk factors.