[Study of stabilized erythrocytes in the direct hemagglutination reaction with influenza viruses by the method of multifactorial planning of experiments]. / Izuchenie stabilizirovannykh éritrotsitov v reaktsii priamoi gemaggliutinatsii s virusami grippa metodom mnogofaktornogo planirovaniia éksperimenta

It was demonstrated that human, chicken and guinea pig erythrocytes stabilized with acrolein, glutaraldehyde and formalin could be used for titration of influenza A1, A2 and B viruses. For this purpose, a four-factor experiment of the type of 3X4X3X2 was planned. All the factors under study (the erythrocyte species, stabilization, erythrocyte concentration and virus type), and some dual interactions (erythrocyte species and stabilization, erythrocyte species and virus type, stabilization and virus type) were found to be significant. Planning of a multifactorial experiment permits a composite investigation of the infleunce of the factors under study and their interactions on influenza virus titers with a reduction of the total number of tests and to obtain more significant answers to preset tasks.