RESUMO
Hundred and fifty-four samples of heparinized blood from patients with chronic toxoplasmosis, chronic brucellosis, lung diseases, psychiatric affective disorders, healthy individuals and neonates, were obtained. The experimental material included also 14 bone marrow samples from patients with hematological diseases or lung cancer. The whole cell populations were treated with several classical IFN inducers and supernatants were assayed for IFN activity in human lung adenocarcinoma cell line A 549 using VSV or EMC viruses as challenge. The response of WBC to NDV or PHA + PMA was high and remarkably stable in majority of cases except listed below. The levels of IFN induced by LPS were low. The response of WBMC to IFN inducers closely resembled the response of WBC. Low IFN levels in samples of stimulated WBMC from some hematological patients were connected with low cellularity. WBC from psychiatric patients with affective disorders weakly responded to stimulation with NDV. This suggested an existence of deficiency of IFN alpha production connected with affective disorders. Also WBC from cord blood failed to react to PHA + PMA and were poorly responsive to NDV.
Assuntos
Medula Óssea/metabolismo , Interferon Tipo I/biossíntese , Interferon gama/biossíntese , Adolescente , Adulto , Transtornos Psicóticos Afetivos/sangue , Transtornos Psicóticos Afetivos/metabolismo , Brucelose/sangue , Brucelose/metabolismo , Doença Crônica , Feminino , Humanos , Recém-Nascido , Indutores de Interferon/farmacologia , Interferon Tipo I/sangue , Interferon gama/sangue , Pneumopatias/sangue , Pneumopatias/metabolismo , Masculino , Pessoa de Meia-Idade , Toxoplasmose/sangue , Toxoplasmose/metabolismoRESUMO
Therapeutic plasmaphereses using CS 3000 Fenwal Cell Separator were performed in 4 women and 2 men, aged between 17 and 44 years, with hepatic coma complicating acute viral hepatitis type B. One to four plasma exchanges per patient were performed, usually at the volume of 3000 ml per procedure. Two patients at II and IVa period of the coma, according to Aboun classification, survived. Four patients at II, III and two at III/IV period of the coma died. The authors suggest that in some cases exchange of large volumes of plasma in the treatment of hepatic coma complicating acute viral hepatitis may be a lifesaving procedure.
Assuntos
Encefalopatia Hepática/terapia , Hepatite B/complicações , Plasmaferese/métodos , Doença Aguda , Adolescente , Adulto , Feminino , Encefalopatia Hepática/etiologia , Encefalopatia Hepática/mortalidade , Humanos , Masculino , Prognóstico , Indução de Remissão , Fatores de TempoRESUMO
Dipyridamole, previously reported to be an interferon (IFN) inducer in mice, was evaluated in 43 normal volunteers or cancer patients. At doses ranging from 150 mg orally to 887 mg intravenously, no serum antiviral activity suggesting IFN induction was observed.