Remediation of copper and lead contaminated sediments using iron-based granule biochar: mechanisms and enzyme activity.

In recent years, there has been a growing concern about heavy metal contamination in sediments. In this study, iron-based granular biochar (MGB) is prepared to remediate Cu and Pb contaminated sediments. Characterizations via scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR) show that the rough surface of MGB with abundant pore structures and a large number of oxygen-containing functional groups that facilitate stabilization of Cu and Pb in sediments. Potential mobility and bioavailability of Cu and Pb are investigated using BCR sequential extraction in the 35 day remediation experiment. The XPS results indicate that FeOOH and C-OH play a crucial part in stabilizing heavy metals. Large affinity of FeOOH for Pb allows it to occupy a proportion in F2 while C-OH is attractive to Cu. Changes of pH, organic matter (OM), and available phosphorus (AP) in sediments after adding MGB as well as the relationship between changes and the stable solidification of Cu and Pb are explored. The stable solidification of heavy metals effectively reduces the available phosphorus in sediments. Magnetic and particle properties of the material are used to reduce the impact of MGB aging on sediment environment and separate it from the remediated sediment. Finally, 3% of MGB significantly enhanced the sediment catalase activity in the biological enzyme activity experiment. All findings indicate that MGB is a green and environmentally friendly sediment remediation material with satisfactory potential in synergistically stabilizing heavy metals and phosphorus.Highlights The complexation of FeOOH with Pb on the surface of MGB fixes it to the reduced stateThe C-OH on the surface of MGB is more attractive to Cu than PbMGB effectively mitigates the release of bioavailable phosphorus from sediments to overlying water.

Molecular Characterization and Expression Profile of PaCOL1, a CONSTANS-like Gene in Phalaenopsis Orchid.

CONSTANS (CO) and CONSTANS-like (COL) genes play important roles in coalescing signals from photoperiod and temperature pathways. However, the mechanism of CO and COLs involved in regulating the developmental stage transition and photoperiod/temperature senescing remains unclear. In this study, we identified a COL ortholog gene from the Taiwan native orchid Phalaenopsis aphrodite. The Phalaenopsis aphrodite CONSTANS-like 1 (PaCOL1) belongs to the B-box protein family and functions in the nucleus and cytosol. Expression profile analysis of Phalaenopsis aphrodite revealed that PaCOL1 was significantly expressed in leaves, but its accumulation was repressed during environmental temperature shifts. We found a differential profile for PaCOL1 accumulation, with peak accumulation at late afternoon and at the middle of the night. Arabidopsis with PaCOL1 overexpression showed earlier flowering under short-day (SD) conditions (8 h/23 °C light and 16 h/23 °C dark) but similar flowering time under long-day (LD) conditions (16 h/23 °C light and 8 h/23 °C dark). Transcriptome sequencing revealed several genes upregulated in PaCOL1-overexpressing Arabidopsis plants that were previously involved in flowering regulation of the photoperiod pathway. Yeast two-hybrid (Y2H) analysis and bimolecular fluorescence complementation (BiFC) analysis revealed that PaCOL1 could interact with a crucial clock-associated regulator, AtCCA1, and a flowering repressor, AtFLC. Furthermore, expressing PaCOL1 in cca1.lhy partially reversed the mutant flowering time under photoperiod treatment, which confirms the role of PaCOL1 function in the rhythmic associated factors for modulating flowering.