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1.
J Pineal Res ; 76(5): e12995, 2024 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-39073181

RESUMO

Melatonin (MT) has been implicated in the plant response to phosphorus (P) stress; however, the precise molecular mechanisms involved remain unclear. This study investigated whether MT controls internal P distribution and root cell wall P remobilization in rice. Rice was treated with varying MT and P levels and analyzed using biochemical and molecular techniques to study phosphorus utilization. The results demonstrated that low P levels lead to a rapid increase in endogenous MT levels in rice roots. Furthermore, the exogenous application of MT significantly improved rice tolerance to P deficiency, as evidenced by the increased biomass and reduced proportion of roots to shoots under P-deficient conditions. MT application also mitigated the decrease in P content regardless in both the roots and shoots. Mechanistically, MT accelerated the reutilization of P, particularly in the root pectin fraction, leading to increased soluble P liberation. In addition, MT enhanced the expression of OsPT8, a gene involved in root-to-shoot P translocation. Furthermore, we observed that MT induced the production of nitric oxide (NO) in P-deficient rice roots and that the mitigating effect of MT on P deficiency was compromised in the presence of the NO inhibitor, c-PTIO, implying that NO is involved in the MT-facilitated mitigation of P deficiency in rice. Overall, our findings highlight the potential of MT as a promising strategy for enhancing rice tolerance to P deficiency and improving P use efficiency in agricultural practices.


Assuntos
Parede Celular , Melatonina , Óxido Nítrico , Oryza , Fósforo , Raízes de Plantas , Oryza/metabolismo , Fósforo/metabolismo , Melatonina/metabolismo , Melatonina/farmacologia , Raízes de Plantas/metabolismo , Raízes de Plantas/efeitos dos fármacos , Parede Celular/metabolismo , Parede Celular/efeitos dos fármacos , Óxido Nítrico/metabolismo
2.
Skin Res Technol ; 30(4): e13693, 2024 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-38572573

RESUMO

BACKGROUND: To further clarify the acne profile of Chinese adult women, we included 1,156,703 adult women. An artificial intelligence algorithm was used to analyze images taken by high-resolution mobile phones to further explore acne levels in Chinese adult women. METHOD: In this study, we assessed the severity of acne by evaluating patients' selfies through a smartphone application. Furthermore, we gathered basic user information through a questionnaire, including details such as age, gender, skin sensitivity, and dietary habits. RESULTS: This study showed a gradual decrease in acne severity from the age of 25 years. A trough was reached between the ages of 40 and 44, followed by a gradual increase in acne severity. In terms of skin problems and acne severity, we have found that oily skin, hypersensitive skin, frequent makeup application and unhealthy dietary habits can affect the severity of acne. For environment and acne severity, we observed that developed city levels, cold seasons and high altitude and strong radiation affect acne severity in adult women. For the results of the AI analyses, the severity of blackheads, pores, dark circles and skin roughness were positively associated with acne severity in adult women. CONCLUSIONS: AI analysis of high-res phone images in Chinese adult women reveals acne severity trends. Severity decreases after 25, hits a low at 40-44, then gradually rises. Skin type, sensitivity, makeup, diet, urbanization, seasons, altitude, and radiation impact acne. Blackheads, pores, dark circles, and skin roughness are linked to acne severity. These findings inform personalized skincare and public health strategies for adult women.


Assuntos
Acne Vulgar , Inteligência Artificial , Adulto , Humanos , Feminino , Acne Vulgar/epidemiologia , Acne Vulgar/complicações , Pele , China/epidemiologia
3.
Ann Plast Surg ; 93(2S Suppl 1): S4-S10, 2024 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-38896857

RESUMO

BACKGROUND: An increasing number of surgical and nonsurgical interventions are available in the field of female genital plastic surgery. The rate of female genital plastic surgery has increased by nearly 220 percent over the past 5 years. Despite several studies on the topic, no relevant bibliometric analysis has been conducted. METHODS: We searched the Web of Science Core Collection for articles related to female genital plastic surgery. CiteSpace 6.1.R2 (Drexel University, USA) and VOSviewer 1.6.10.0 (Leiden University, the Netherlands) were used, and national distribution, institutions, journals, authors, and key words were analyzed and calculated. RESULTS: From 2003 to 2022, 1299 papers in the field of female genital plastic surgery were retrieved. There were more articles produced in the United States, and there were also two institutions in the Netherlands that were highly productive. A wide and close relationship has been established between researchers and institutions conducting female genital plastic surgery. Professor Bouman MB published the most articles on female genital plastic surgery in the Journal of Sexual Medicine. Female genital plastic surgery dominated the top 10 references with the highest local citation score. There were four clusters of key words with the most citations, and the most recently trending key words were "vaginal agenesis," "transgender," and "congenital adrenal hyperplasia." CONCLUSIONS: The purpose of this article is to provide a summary of the current research status focusing on female genital plastic surgery. It is hoped that more efforts will be made to promote the development of female genital plastic surgery in the future.


Assuntos
Bibliometria , Cirurgia Plástica , Humanos , Feminino , Cirurgia Plástica/estatística & dados numéricos , Cirurgia Plástica/tendências , Procedimentos de Cirurgia Plástica/estatística & dados numéricos , Procedimentos Cirúrgicos em Ginecologia/estatística & dados numéricos , Genitália Feminina/cirurgia
4.
Int Wound J ; 21(1): e14559, 2024 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-38272806

RESUMO

Localised scleroderma predominantly affects the skin with an unknown aetiology. Despite its clinical importance, no comprehensive bibliometric analysis has been conducted to assess the existing research landscape and future prospects for localised scleroderma. The articles related to localised scleroderma were retrieved from the WoSCC database and analysed by VOSviewer 1.6.10.0 (Leiden University, Netherlands), CiteSpace 6.1.R1 (Dreiser University, USA), and HistCite 2.1 (New York, United States). 2049 research papers pertaining to localised scleroderma spanning the years from 1993 to 2022 were extracted from the WoSCC database. The United States exhibited the highest productivity with 644 papers, accounting for 31.43% of the total output, followed by Germany with 206 papers (10.05%) and Italy with 200 papers (9.76%). Regarding academic institutions and journals, the University of Texas System and Dermatology published the most significant number of papers, and Professor Ihn, H emerged as the most prolific contributor among scholars. The top 10 cited references primarily concentrated on the diagnosis and treatment of localised scleroderma. "Phototherapy" and "methotrexate (MTX)" surfaced as the most recent and noteworthy keywords, representing the research hotspots in the domain of localised scleroderma. This bibliometric analysis furnishes valuable insights into the contemporary research landscape of localised scleroderma.


Assuntos
Esclerodermia Localizada , Humanos , Esclerodermia Localizada/terapia , Pele , Bibliometria , Bases de Dados Factuais , Alemanha
5.
J Org Chem ; 88(13): 7998-8009, 2023 07 07.
Artigo em Inglês | MEDLINE | ID: mdl-37279456

RESUMO

An unexpected Ugi cascade reaction was developed for the facile construction of γ-lactam-fused pyridone derivatives with high tolerance of substrates. A C(sp3)-N bond and a C(sp2)-C(sp2) bond were formed together, accompanied by a chromone ring-opening in Ugi adducts, under the basic conditions without any metal catalyst for the whole process. Screening data of several difficult-to-inhibit cancer cell lines demonstrated that 7l displayed a high cytotoxicity against HCT116 cells (IC50 = 5.59 ± 0.78 µM). Taken together, our findings revealed new insights into the molecular mechanisms underlying compound 7l and provided potential usage of this scaffold for cancer therapeutics.


Assuntos
Compostos Heterocíclicos , Lactamas , Lactamas/farmacologia , Piridonas/farmacologia , Piridonas/química , Metais
6.
Skin Res Technol ; 29(11): e13492, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38009029

RESUMO

BACKGROUND: To better compare the progression of dark circles and the aging process in Chinese skin. A total of 100 589 Chinese males and 1 838 997 Chinese females aged 18 to 85, without facial skin conditions, and who had access to a smartphone with a high-resolution camera all took selfies. METHOD: Using a smartphone application with a built-in artificial intelligence algorithm, facial skin diagnostic evaluated the selfies and score the severity of the dark circles with four other facial indicators (including skin type, Pores, Acne vulgaris, and Blackheads). Basic information was collected with online questionnaire, including their age, gender, skin sensitivity, and dietary habits. RESULTS: In users between the age of 18 and 59, the prevalence of comprehensive, pigmented, and structural type of dark circles all rose with age. However, between the age of 60 and 85, the intensity of all types of dark circles diminished. Besides, vascular dark circles progressively worsen from the age of 18 to their peak at 39, and then gradually decline with age. Females typically have more pronounced black circles under their eyes than males in China. Bad eating habits, urbanization, regular cosmetics use, and sensitive skin positively correlate with severe dark circles. Vascular, comprehensive dark circles were worse in spring. Both pigmented and structural dark circles were worse in the summer. The results indicated that the intensity of dark circles was influenced by oily skin, wide pores, severe blackheads, and severe acne. CONCLUSIONS: Chinese men and women differed noticeably in the prevalence of each face aging indicator and the appearance of aging dark circles. Selfies could be automatically graded and examined by artificial intelligence, which is a quick and private method for quantifying signs of facial aging and identifying major problems for different populations. Artificial intelligence would assist in the development of individualized preventive and therapeutic interventions.


Assuntos
Inteligência Artificial , Face , Envelhecimento da Pele , Feminino , Humanos , Masculino , Acne Vulgar , População do Leste Asiático , Pele , Adolescente , Adulto Jovem , Adulto , Pessoa de Meia-Idade , Idoso , Idoso de 80 Anos ou mais
7.
Aesthetic Plast Surg ; 47(3): 1192-1204, 2023 06.
Artigo em Inglês | MEDLINE | ID: mdl-36460771

RESUMO

BACKGROUND: Fat grafting is one of the most effective treatments for soft tissue restoration and augmentation. Adipose-derived stem cells (ASCs) supplementation is one of the foremost concerns to improve its efficiency. There have been several studies aiming at adipose-derived mesenchymal stem cells in fat grafting, but no relevant bibliometric research has conducted. METHODS: Articles about fat grafting and ASCs were retrieved in Web of Science Core Collection (WoSCC). Using VOSviewer 1.6.10.0 (Leiden University, the Netherlands) and CiteSpace 6.1.R2 (Drexel University, USA), the information of national distribution, institutions, journals, authors and keywords were evaluated and calculated. RESULTS: A total of 1166 papers in the field of ASCs in fat grafting were retrieved from 2002 to 2021. The USA produced the most articles, and the top 2 productive institutions were all from the USA. Researchers and institutions conducting ASCs in fat grafting research have shown a widespread and close connection. Plastic and Reconstructive Surgery published the most article on ASCs in fat grafting, and professor Rubin Peter is the most productive author. The top 10 references with the highest LCS mainly focused on applying ASCs to assist fat transplantation in plastic surgery. The most cited keywords formed 4 clusters, and "mesenchymal stem," "mesenchymal stromal cell," "stromal vascular fraction" and "long term" were the most recently trending keywords. CONCLUSIONS: This article provides a summary of the current research status focusing on fat grafting and ASCs. More efforts will be made to promote the application of ASCs in fat grafting. LEVEL OF EVIDENCE V: This journal requires that authors assign a level of evidence to each article. For a full description of these Evidence-Based Medicine ratings, please refer to the Table of Contents or the online Instructions to Authors www.springer.com/00266 .


Assuntos
Adipócitos , Procedimentos de Cirurgia Plástica , Humanos , Adipócitos/transplante , Bibliometria , Tecido Adiposo/transplante , Células-Tronco
8.
J Environ Manage ; 326(Pt B): 116836, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36435130

RESUMO

Renewable energy generation varies frequently, making it difficult to match electricity demand. Pumped storage hydropower plants can alleviate this problem by reducing the unevenness of renewable energy generation. It is a new exploration of energy storage methods to construct pumped storage hydropower plants by using underground goaf of abandoned mines and mining subsidence water area. However, the construction of lower reservoirs using underground goaf areas of abandoned mines can lead to potential heavy metal pollution. To assess the impact of using abandoned mines for pumped storage hydropower on the downstream surface water environment, this work first analyzed the release characteristics of heavy metals from underground goaf and surface dump through field sampling and leaching tests, then constructed a water-environment model of the downstream surface water based on the experimental results and water environment theory, and finally simulated and analyzed the impact of underground pollutants pumped to the surface on downstream surface water-quality in typical hydrological years. The maximum error between the simulated values and measured values of the hydrodynamic model was 0.1, and the overall error was within a reasonable range (±0.2 m). The comparison between simulated values and measured values of heavy metal concentration in water quality model showed RMSE values ranged from 0.003 to 0.81, with an average of 0.4; the SI ranged from 0.84 to 0.95, with an average of 0.89. During the simulation of low, normal, and high flow years, pollution downstream was concentrated near the drainage outlet, and the underground pollutants pumped to the surface influenced the concentration of heavy metals there. After a period of drainage, the concentration of heavy metals decreased. Drainage volume was an important factor affecting the concentration of heavy metals downstream surface water. These results prove that the water environment model established based on MIKE21 is reliable and can provide guidance for the simulation and control of heavy metal pollution in the utilization of abandoned mines for pumped storage hydropower. This work provides a reproducible idea and method to assess the impact of using abandoned mines and mining subsidence water area for pumped storage hydropower on downstream surface water and ensure the safety of the ecological environment.


Assuntos
Poluentes Ambientais , Metais Pesados , Poluentes do Solo , Mineração , Metais Pesados/análise , Poluição Ambiental , Meio Ambiente , Monitoramento Ambiental , Poluentes do Solo/análise
9.
J Environ Manage ; 348: 119335, 2023 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-37857212

RESUMO

Addressing the widespread concern of chromium (Cr) pollution, this study investigated its impacts on bacterial communities across eight soil types, alongside the potential Cr transformation-related genes. Utilizing real-time PCR, 16S rRNA gene sequencing and gene prediction, we revealed shifts in bacterial community structure and function at three Cr exposure levels. Our results showed that the bacterial abundance in all eight soil types was influenced by Cr to varying extents, with yellow‒brown soil being the most sensitive. The bacterial community composition of different soil types exhibited diverse responses to Cr, with only the relative abundance of Proteobacteria decreasing with increasing Cr concentration across all soil types. Beta diversity analysis revealed that while Cr concentration impacted the assembly process of bacterial communities to a certain extent, the influence on the compositional structure of bacterial communities was primarily driven by soil type rather than Cr concentration. The study also identified biomarkers for each soil type under three Cr levels, offering a basis for monitoring changes in Cr pollution. By predicting crucial functional genes related to Cr transformation, it was observed that the relative abundance of chrA (chromate transporter) in yellow‒brown soil significantly exceeded that in all other soil types, suggesting its potential for Cr adaptation. The study also revealed correlations among soil physicochemical properties, Cr concentration, and these functional genes, providing a foundation for future research aimed at more precise functional analysis and the development of effective soil remediation strategies.


Assuntos
Cromo , Poluentes do Solo , Cromo/análise , Solo/química , RNA Ribossômico 16S/genética , Bactérias/genética , Proteobactérias/genética , Microbiologia do Solo , Poluentes do Solo/análise
10.
Molecules ; 28(7)2023 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-37049976

RESUMO

Colorectal cancer (CRC) is the most common intestinal malignancy, and nearly 70% of patients with this cancer develop metastatic disease. In the present study, we synthesized a novel compound, termed N-(3-(5,7-dimethylbenzo [d]oxazol-2-yl)phenyl)-5-nitrofuran-2-carboxamide (compound 275#), and found that it exhibits antiproliferative capability in suppressing the proliferation and growth of CRC cell lines. Furthermore, compound 275# triggered caspase 3-mediated intrinsic apoptosis of mitochondria and autophagy initiation. An investigation of the molecular mechanisms demonstrated that compound 275# induced intrinsic apoptosis, and autophagy initiation was largely mediated by increasing the levels of the intracellular accumulation of reactive oxygen species (ROS) in CRC cells. Taken together, these data suggest that ROS accumulation after treatment with compound 275# leads to mitochondria-mediated apoptosis and autophagy activation, highlighting the potential of compound 275# as a novel therapeutic agent for the treatment of CRC.


Assuntos
Apoptose , Neoplasias Colorretais , Humanos , Espécies Reativas de Oxigênio/metabolismo , Linhagem Celular Tumoral , Mitocôndrias/metabolismo , Autofagia , Neoplasias Colorretais/patologia , Proliferação de Células
11.
Molecules ; 28(4)2023 Feb 17.
Artigo em Inglês | MEDLINE | ID: mdl-36838936

RESUMO

In this study, 2-benzyl-10a-(1H-pyrrol-2-yl)-2,3-dihydropyrazino[1,2-a]indole-1,4,10(10aH)-trione (DHPITO), a previously identified inhibitor against hepatocellular carcinoma cells, is shown to exert its cytotoxic effects by suppressing the proliferation and growth of CRC cells. An investigation of its molecular mechanism confirmed that the cytotoxic activity of DHPITO is mediated through the targeting of microtubules with the promotion of subsequent microtubule polymerisation. With its microtubule-stabilising ability, DHPITO also consistently arrested the cell cycle of the CRC cells at the G2/M phase by promoting the phosphorylation of histone 3 and the accumulation of EB1 at the cell equator, reduced the levels of CRC cell migration and invasion, and induced cellular apoptosis. Furthermore, the compound could suppress both tumour size and tumour weight in a CRC xenograft model without any obvious side effects. Taken together, the findings of the present study reveal the antiproliferative and antitumour mechanisms through which DHPITO exerts its activity, indicating its potential as a putative chemotherapeutic agent and lead compound with a novel structure.


Assuntos
Antineoplásicos , Neoplasias Colorretais , Humanos , Linhagem Celular Tumoral , Tubulina (Proteína)/metabolismo , Pontos de Checagem do Ciclo Celular , Apoptose , Moduladores de Tubulina/farmacologia , Microtúbulos , Antineoplásicos/farmacologia , Neoplasias Colorretais/metabolismo , Proliferação de Células
12.
Int J Mol Sci ; 23(15)2022 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-35955772

RESUMO

Environmental metal pollution is a common problem threatening sustainable and safe crop production. Heavy metals (HMs) cause toxicity by targeting key molecules and life processes in plant cells. Plants counteract excess metals in the environment by enhancing defense responses, such as metal chelation, isolation to vacuoles, regulating metal intake through transporters, and strengthening antioxidant mechanisms. In recent years, microRNAs (miRNAs), as a small non-coding RNA, have become the central regulator of a variety of abiotic stresses, including HMs. With the introduction of the latest technologies such as next-generation sequencing (NGS), more and more miRNAs have been widely recognized in several plants due to their diverse roles. Metal-regulated miRNAs and their target genes are part of a complex regulatory network. Known miRNAs coordinate plant responses to metal stress through antioxidant functions, root growth, hormone signals, transcription factors (TF), and metal transporters. This article reviews the research progress of miRNAs in the stress response of plants to the accumulation of HMs, such as Cu, Cd, Hg, Cr, and Al, and the toxicity of heavy metal ions.


Assuntos
Metais Pesados , MicroRNAs , Antioxidantes , Metais Pesados/toxicidade , MicroRNAs/genética , Plantas/genética , Estresse Fisiológico/genética
13.
Phys Chem Chem Phys ; 24(1): 30-34, 2021 Dec 22.
Artigo em Inglês | MEDLINE | ID: mdl-34882162

RESUMO

The existence of an inflection point between fullerene and nonfullerene molecules was demonstrated using bowl-nonfullerene models, which were proposed to explore the key points relating to the different properties. This study explains the differences in the stacking configurations of the inflection points and reveals the reason for the inversion of properties, which is caused by small structural differences.

14.
Biol Res ; 54(1): 27, 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34488902

RESUMO

BACKGROUND: Demethylzeylasteral (T-96) is a pharmacologically active triterpenoid monomer extracted from Tripterygium wilfordii Hook F (TWHF) that has been reported to exhibit anti-neoplastic effects against several types of cancer cells. However, the potential anti-tumour effects of T-96 against human Prostate cancer (CaP) cells and the possible underlying mechanisms have not been well studied. RESULTS: In the current study, T-96 exerted significant cytotoxicity to CaP cells in vitro and induced cell cycle arrest at S-phase in a dose-dependent manner. Mechanistically, T-96 promoted the initiation of autophagy but inhibited autophagic flux by inducing ROS-mediated endoplasmic reticulum (ER) stress which subsequently activated the extrinsic apoptosis pathway in CaP cells. These findings implied that T-96-induced ER stress activated the caspase-dependent apoptosis pathway to inhibit proliferation of CaP cells. Moreover, we observed that T-96 enhances the sensitivity of CaP cells to the chemotherapeutic drug, cisplatin. CONCLUSIONS: Taken together, our data demonstrated that T-96 is a novel modulator of ER stress and autophagy, and has potential therapeutic applications against CaP in the clinic.


Assuntos
Autofagia , Neoplasias da Próstata , Apoptose , Linhagem Celular Tumoral , Humanos , Masculino , Neoplasias da Próstata/tratamento farmacológico , Espécies Reativas de Oxigênio , Triterpenos
15.
Chin Med Sci J ; 36(4): 295-306, 2021 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-34986966

RESUMO

Objective To study the reliability and validity of the Chinese version of the Lymphedema Quality of Life Questionnaire (LYMQOL) in lymphedema patients. Methods LYMQOL was translated into Chinese. The Chinese version of the LYMQOL was distributed with the official Wechat account "Lymphedema Channel" to lymphedema patients who were recruited from October 28 th, 2020 to February 23rd, 2021. Patients with upper limb lymphedema and lower limb lymphedema completed the LYMQOL-ARM subscale and the LYMQOL-LEG subscale separately, at enrollment, 1 week later, and 1 month later. Reliability, validity, feasibility, responsiveness and average time required for completing the questionnaire were assessed. Results A total of 195 patients participated in the study. The Chinese questionnaire showed high reliability with Cronbach's α coefficients of 0.849-0.902 for the LYMQOL-ARM and intraclass correlation coefficient (ICC) of 0.848-0.884 and Cronbach's α coefficients of 0.726-0.902 for the LYMQOL-LEG and ICC of 0.863-0.900. The LYMQOL showed moderate to good correlations with the EQ-5D (0.4

Assuntos
Linfedema , Qualidade de Vida , China , Humanos , Reprodutibilidade dos Testes , Inquéritos e Questionários
16.
J Org Chem ; 84(19): 12632-12638, 2019 10 04.
Artigo em Inglês | MEDLINE | ID: mdl-31357859

RESUMO

A facile and metal-free one-pot protocol for the synthesis of fused imidazopyridine scaffolds has been developed. This novel protocol combines the Groebke-Blackburn-Bienaymé reaction (GBBR) with a sequential TBAB-mediated cyclization cascade. Biological evaluation demonstrated that compound 6a inhibits human prostate cancer cell DU-145 proliferation with an IC50 of 1.6 µM. The molecular mechanism study indicates that 6a significantly suppresses the oncogenic Erk kinase phosphorylation at 3 µM.


Assuntos
Antineoplásicos/farmacologia , Imidazóis/farmacologia , Piridinas/farmacologia , Compostos de Amônio Quaternário/farmacologia , Antineoplásicos/síntese química , Antineoplásicos/química , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , Cristalografia por Raios X , Ciclização , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Imidazóis/química , Micro-Ondas , Modelos Moleculares , Estrutura Molecular , Piridinas/química , Compostos de Amônio Quaternário/química
17.
Sensors (Basel) ; 19(2)2019 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-30642019

RESUMO

To reduce the emissions and weight of vehicles, manufacturers are incorporating polymer materials into vehicles, and this has increased the difficulty in recycling End-of-Life vehicles (ELVs). About 25⁻30% (mass) of an ELV crushed mixture is the unrecyclable material known as automotive shredder residues (ASRs), and most of the vehicle polymers are concentrated in this fraction. Thus, these vehicle polymers are conventionally disposed of in landfills at a high risk to the environment. The only way to solve this problem is through the development of a novel separation and recycling mechanism for ASRs. Our previous research reported a novel sensor-aided single-scrap-oriented sorting method that uses laser-triangulation imaging combined with impact acoustic frequency recognition for sorting crushed ASR plastics, and we proved its feasibility. However, the sorting efficiencies were still limited, since, in previous studies, the method used for scrap size determination was mechanical sieving, resulting in many deviations. In this paper, a new method based on three-dimensional (3D) imaging and circularity analysis is proposed to determine the equivalent particle size with much greater accuracy by avoiding the issues that are presented by the irregularity of crushed scraps. In this research, two kinds of commonly used vehicle plastics, acrylonitrile-butadiene-styrene (ABS) and polypropylene (PP), and their corresponding composite materials, acrylonitrile-butadiene-styrene/polycarbonate (ABS/PC) and polypropylene/ethylene-propylene-diene-monomer (PP/EPDM), were studied. When compared with our previous study, with this new method, the sorting efficiency increased, with PP and PP/EPDM and ABS and ABS/PC achieving about 15% and 20% and 70% and 90%, respectively. The sorting efficiency of ASR polymer scraps can be optimized significantly by using sensor-aided 3D image measurement and circularity analysis.

18.
Molecules ; 24(15)2019 Jul 26.
Artigo em Inglês | MEDLINE | ID: mdl-31357480

RESUMO

We have previously shown that compound-7g inhibits colorectal cancer cell proliferation and survival by inducing cell cycle arrest and PI3K/AKT/mTOR pathway blockage. However, whether it has the ability to exert antitumor activity in other cancer cells and what is the exact molecular mechanism for its antiproliferation effect remained to be determined. In the present study, compound-7g exhibited strong activity in suppressing proliferation and growth of glioblastoma cells. The inhibitor selectively downregulated F-box protein SKP2 expression and upregulated cell cycle inhibitor p27, and then resulted in G1 cell cycle arrest. Mechanism analysis revealed that compound-7g also provokes the down-regulation of E2F-1, which acts as a transcriptional factor of SKP2. Further results indicated that compound-7g induced an increase of LC3B-II and p62, which causes a suppression of fusion between autophagosome and lysosome. Moreover, compound-7g mediated autophagic flux blockage promoted accumulation of ubiquitinated proteins and then led to endoplasmic reticulum stress. Our study thus demonstrated that pharmacological inactivation of E2F-1-SKP2-p27 axis is a promising target for restricting cancer progression.


Assuntos
Antineoplásicos/química , Antineoplásicos/farmacologia , Benzimidazóis/química , Isoquinolinas/química , Proteínas Quinases Associadas a Fase S/genética , Autofagia/efeitos dos fármacos , Linhagem Celular Tumoral , Fator de Transcrição E2F1/metabolismo , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Glioblastoma/genética , Glioblastoma/metabolismo , Humanos , Proteólise
19.
BMC Genet ; 19(1): 12, 2018 02 13.
Artigo em Inglês | MEDLINE | ID: mdl-29439656

RESUMO

BACKGROUND: Loropetalum subcordatum is an endangered species endemic to China that is characterized by narrow distribution, small population size, and delayed fertilization. However, the genetic diversity of the entire extant natural and ex situ populations has not been assessed to date. In this study, we evaluated the genetic diversity and structure of six natural populations and a single ex situ population (the only known ex situ population of L. subcordatum) using sequence-related amplified polymorphism data. RESULTS: In total, 553 reliable DNA bands, of which 359 (63.28%) were polymorphic, were amplified by polymerase chain reaction with combinations of 15 primers. Low average gene diversity within populations and high genetic differentiation were detected in L. subcordatum. A Mantel test demonstrated that there was a positive correlation between genetic and geographic distances, indicating that significant genetic divergence was likely the result of geographic isolation among natural populations. Furthermore, based on genetic structure patterns, populations of L. subcordatum were divided into three clusters. Group 1 was composed of specimens from Libo, Guizhou Province (GZ) and Huanjiang, Guangxi Zhuang Autonomous Region (GX). Group 2 was composed of Mt. Wuguishan, Guangdong Province (GD). Group 3 was composed of three populations in Hong Kong Special Administrative Region. Additionally, clonal reproduction probably existed in GD population. According to the genetic information analysis and field survey, the ex situ population did not match its source population (GD) in terms of genetics, and its habitat was different from the original natural habitat. We observed that a few individual GD seeds were needed to improve ZS ex situ in the future. CONCLUSIONS: Compared to previous SRAP-based studies of endangered plants, L. subcordatum had extremely low average gene diversity within populations and high genetic differentiation among populations. At present, the unique ex situ population has not been successful due to non-representative samples being taken, a smaller population size, and man-made changes in habitat. Potential strategies are suggested to improve the conservation of this species.


Assuntos
Espécies em Perigo de Extinção , Hamamelidaceae/genética , Filogeografia , Polimorfismo Genético , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados/métodos , China , Hamamelidaceae/classificação , Densidade Demográfica
20.
Sensors (Basel) ; 18(5)2018 Apr 27.
Artigo em Inglês | MEDLINE | ID: mdl-29702564

RESUMO

With the increase the worldwide consumption of vehicles, end-of-life vehicles (ELVs) have kept rapidly increasing in the last two decades. Metallic parts and materials of ELVs can be easily reused and recycled, but the automobile shredder residues (ASRs), of which elastomer and plastic materials make up the vast majority, are difficult to recycle. ASRs are classified as hazardous materials in the main industrial countries, and are required to be materially recycled up to 85⁻95% by mass until 2020. However, there is neither sufficient theoretical nor practical experience for sorting ASR polymers. In this research, we provide a novel method by using S-Band microwave irradiation together with 3D scanning as well as infrared thermal imaging sensors for the recognition and sorting of typical plastics and elastomers from the ASR mixture. In this study, an industrial magnetron array with 2.45 GHz irradiation was utilized as the microwave source. Seven kinds of ELV polymer (PVC, ABS, PP, EPDM, NBR, CR, and SBR) crushed scrap residues were tested. After specific power microwave irradiation for a certain time, the tested polymer materials were heated up to different extents corresponding to their respective sensitivities to microwave irradiation. Due to the variations in polymer chemical structure and additive agents, polymers have different sensitivities to microwave radiation, which leads to differences in temperature rises. The differences of temperature increase were obtained by a thermal infrared sensor, and the position and geometrical features of the tested scraps were acquired by a 3D imaging sensor. With this information, the scrap material could be recognized and then sorted. The results showed that this method was effective when the tested polymer materials were heated up to more than 30 °C. For full recognition of the tested polymer scraps, the minimum temperature variations of 5 °C and 10.5 °C for plastics and elastomers were needed, respectively. The sorting efficiency was independent of particle sizes but depended on the power and time of the microwave irradiation. Generally, more than 75% (mass) of the tested polymer materials could be successfully recognized and sorted under an irradiation power of 3 kW. Plastics were much more insensitive to microwave irradiation than elastomers. With this method, the tested mixture of the plastic group (PVC, ABS, PP) and the mixture of elastomer group (EPDM, NBR, CR, and SBR) could be fully separated with an efficiency of 100%.

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