Developing early lexical composition in Mandarin-speaking children: A longitudinal study.

This study investigated the developmental pattern of early lexical production and composition in Mandarin-speaking children. Forty Mandarin-speaking children and their parents participated in this one-and-a-half-year longitudinal study, and naturalistic samples of parent-to-child speech in toy play were collected when the children were 1;8, 2;2, and 3;0. The results showed that children's lexical production increased significantly between ages 1;8 and 3;0. The proportion of closed-class words increased significantly with age, whereas the proportion of common nouns showed the inverse pattern, indicating the role of grammatical words increased as the children grew. Furthermore, nouns and verbs were predominant in Mandarin-speaking children between ages 1;8 and 3;0, and Mandarin-speaking children used more verbs than nouns at 2;2 and 3;0 in the toy play context. The longitudinal study clarifies early lexical development in Mandarin-speaking children, which provides a valuable contrast for different language systems.

Gut microbiome changes in overweight male adults following bowel preparation.

BACKGROUND: Human gut microbiome has an essential role in human health and disease. Although the major dominant microbiota within individuals have been reported, the change of gut microbiome caused by external factors, such as antibiotic use and bowel cleansing, remains unclear. We conducted this study to investigate the change of gut microbiome in overweight male adults after bowel preparation, where none of the participants had been diagnosed with any systemic diseases. METHODS: A total of 20 overweight, male Taiwanese adults were recruited, and all participants were omnivorous. The participants provided fecal samples and blood samples at three time points: prior to bowel preparation, 7 days after colonoscopy, and 28 days after colonoscopy. The microbiota composition in fecal samples was analyzed using 16S ribosome RNA gene amplicon sequencing. RESULTS: Our results demonstrated that the relative abundance of the most dominant bacteria hardly changed from prior to bowel preparation to 28 days after colonoscopy. Using the ratio of Prevotella to the sum of Prevotella and Bacteroides in the fecal samples at baseline, the participants were separated into two groups. The fecal samples of the Type 1 group was Bacteroides-dominant, and that of the Type 2 group was Prevotella-dominant with a noticeable presence Bacteroides. Bulleidia appears more in the Type 1 fecal samples, while Akkermensia appears more in the Type 2 fecal samples. Of each type, the gut microbial diversity differed slightly among the three collection times. Additionally, the Type 2 fecal microbiota was temporarily susceptible to bowel cleansing. Predictive functional analysis of microbial community reveals that their activities for the mineral absorption metabolism and arachidonic acid metabolism differed significantly between the two types. Depending on their fecal type, the variance of triglycerides and C-reactive protein also differed between the two types of participants. CONCLUSIONS: Depending upon the fecal type, the microbial diversity and the predictive functional modules of microbial community differed significantly after bowel preparation. In addition, blood biochemical markers presented somewhat associated with fecal type. Therefore, our results might provide some insights as to how knowledge of the microbial community could be used to promote health through personalized clinical treatment.

The performance and predictors of Chinese character writing in children with attention deficit hyperactivity disorder.

Writing difficulties are common in children with attention deficit hyperactivity disorder (ADHD). Preliminary evidence suggests that early character writing ability is fundamental for later writing composition and academic achievement. Critical factors of different character writing tasks in children with ADHD, however, remain unclear. This study aims to describe the performance and identify predictors of Chinese character writing in children with ADHD. Thirty Mandarin Chinese-speaking children with ADHD (7.16 ± 0.59 years) and thirty matched peers (7.21 ± 0.57 years) were recruited from northern Taiwan. They were evaluated with the Battery of Chinese Basic Literacy (BCBL); the Chinese version of the Test of Nonverbal Intelligence, fourth edition (C-TONI-4); the Chinese version of the Peabody Picture Vocabulary Test-Revised (C-PPVT-R); orthographic awareness test; character naming test; and the Bruininks Oseretsky Test of Motor Proficiency, second edition (BOT-2). The results showed that Mandarin Chinese-speaking children with ADHD scored lower than their typically developing peers on both dictation and copying subtests. After controlling for age, orthographic awareness and inattention were identified as important predictors of character dictation; while, manual dexterity was a critical predictor of character copying in children with ADHD. The results demonstrated that character writing problems may exist in Mandarin Chinese-speaking children with ADHD, and cognitive-linguistic and fine-motor skills have varying contributions to Chinese character writing tasks.

A Facile Method for Batch Preparation of Electrochemically Reduced Graphene Oxide.

The electrochemical reduction of graphene oxide (GO) is an environmentally friendly and energy-saving method for improving the characteristics of GO. However, GO films must be coated on the cathode electrode in advance when usingthis technique. Thus, the formed electrochemically reduced GO (ERGO) films can be used only as sensing or conductive materials in devices because mass production of the flakes is not possible. Therefore, this study proposes a facile electrochemical reduction technique. In this technique, GO flakes can be directly used as reduced materials, and no GO films are required in advance. A 0.1 M phosphate buffered saline solution was used as the electrolyte, which is a highly safe chemical agent. Experimental results revealed that the as-prepared GO flakes were electrochemically reduced to form ERGO flakes by using a -10 V bias for 8 h. The ratio of the D-band and G-band feature peaks was increased from 0.86 to 1.12, as revealed by Raman spectroscopy, the π-π stacking interaction operating between the ERGO and GO has been revealed by UV-Vis absorption spectroscopy, and the C⁻O ratio was increased from 2.02 to 2.56, as indicated by X-ray photoelectron spectroscopy. The electrical conductivity of the ERGO film (3.83 × 10-1 S·cm-1) was considerably better than that of the GO film (7.92 × 10-4 S·cm-1). These results demonstrate that the proposed electrochemical reduction technique can provide high-quality ERGO flakes and that it has potential for large-scale production.