RESUMO
Capybaras (Hidrochoeris hidrochoeris) are 1 of the largest rodents used for meat in South and Central America. Prevalence of anti-Toxoplasma gondii antibodies in 149 feral H. hidrochoeris from the state of São Paulo, Brazil, was evaluated using the indirect immunofluorescent antibody test (IFAT) and the modified agglutination test (MAT). Using IFAT, antibodies (>1:16) were found in 104 (69.8%) and with the MAT, antibodies (>1:25) were found in 63 (42.3%) capybaras. This is the first report of prevalence of T. gondii antibodies in this host.
Assuntos
Anticorpos Antiprotozoários/sangue , Doenças dos Roedores/epidemiologia , Toxoplasma/imunologia , Toxoplasmose Animal/epidemiologia , Testes de Aglutinação/veterinária , Animais , Brasil/epidemiologia , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Carne/parasitologia , Doenças dos Roedores/parasitologia , Doenças dos Roedores/transmissão , Roedores , Estudos Soroepidemiológicos , Toxoplasmose Animal/transmissãoRESUMO
Dogs sera samples collected from Cotia County, São Paulo were tested using indirect immunoenzymatic test (ELISA) in order to study Lyme disease serology in dogs. ELISA method was standardized and G39/40 North American strain of Borrelia burgdorferi was used as antigen. Positive results were confirmed employing the Western blotting technique. Because of the possibility of cross-reactions, sera were also tested for different serological strains of Leptospira interrogans and L. biflexa using microscopic sera agglutination test. Twenty-three of 237 (9.7%) serum samples were positive in the ELISA; 20 of them (86.9%) were confirmed by the Western blotting, what suggests that Cotia may be a risk area for Lyme disease. Although 4 samples (1.7%) were positive for Lyme disease and leptospirosis, no correlation was found between the results (X(2) = 0.725; p = 0.394) what suggests absence of serological cross reactivity.
Assuntos
Anticorpos Antibacterianos/isolamento & purificação , Borrelia burgdorferi/imunologia , Doenças do Cão/microbiologia , Doença de Lyme/veterinária , Animais , Western Blotting , Brasil/epidemiologia , Distribuição de Qui-Quadrado , Doenças do Cão/diagnóstico , Doenças do Cão/epidemiologia , Cães , Ensaio de Imunoadsorção Enzimática , Feminino , Doença de Lyme/diagnóstico , Doença de Lyme/epidemiologia , Masculino , Estudos SoroepidemiológicosRESUMO
The prevalence of rabies neutralizing antibodies (NA) in sera of wild animals from São Paulo City (Brazil) was investigated using the Rapid Fluorescent Focus Inhibition Test between 1994 and 1997. Sera from 547 specimens were examined. Marsupials represented 45% of the sample and primates 37%; carnivores, rodents, deer and edentates represented 6, 6, 3 and 2%, respectively. The overall prevalence of NA was 14%. The prevalence of NA was 18% in primates; whereas in marsupials, carnivores, edentates and rodents it was 13, 9, 8 and 6%, respectively. The stratification according to sex, age, and site of capture of the marsupials and primates showed a small predominance in males versus females and a large predominance of adults versus juveniles. The same relationship was seen in specimens captured near human habitations versus specimens captured in their own habitat. It is evident that there is circulation of rabies virus in wild animals, which are not recommended as pets since they represent a potential risk of exposure to rabies virus for both humans and domestic animals.