Enhancing productivity and sustainability of ravine lands through horti-silviculture and soil moisture conservation: A pathway to land degradation neutrality.

Ravine lands are the worst type of land degradation affecting soil quality and biodiversity. Crop production in such lands is impossible without adopting proper conservation measures. In-situ moisture conservation techniques could play an instrumental role in restoring ravine lands by improving soil moisture. We hypothesized that restoring ravine land through a combination of tree planting, fruit crop cultivation, and in-situ moisture conservation practice would result in significant improvements in productivity, profitability, and soil fertility. An experiment was conducted involving the combination of Malabar Neem (Melia dubia) and Dragon fruit (Hylocereus undatus) in conjunction with in-situ soil moisture conservation measures specifically involving half-moon structures (HM). The experiment was conducted under randomized block design (RBD) comprising eight treatments. These treatments include sole Melia cultivation (MD 3m × 3m), sole cultivation of dragon fruit (DF 3m × 3m), silviculture system (MDF-3m × 3m), horti-silviculture system with larger spacing (MDF-4m × 4m), sole Melia cultivation with in-situ moisture conservation (MDH-3m × 3m), sole Dragon fruit cultivation with in-situ moisture conservation (DFH-3m × 3m), horti-silviculture system of Melia and Dragon fruit with in-situ moisture conservation (MDFH-3m × 3m), and horti-silviculture system with larger spacing and in-situ moisture conservation (MDFH-4m × 4m). Each treatment was replicated thrice to evaluate their impact on productivity, profitability, soil fertility, and carbon sequestration for 8 years (2016-2023). The results revealed that the horti-silviculture system (MDFH-3 × 3 m) exhibited the highest total tree biomass and total carbon sequestration with an increase of 183.2% and 82.8% respectively, compared to sole Melia cultivation without HM and sole Melia with HM. Furthermore, sole Melia with HM augmented soil nutrients (N, P, K, and SOC) by 74.4%, 66.4%, 35.2%, and 78.3%, respectively, compared to control (no planting), with performance at par with MDFH-3 × 3 m. Similarly, sole Melia with HM enhanced SOC stock and SOC sequestration rate by 79.2% and 248% over control. However, it was found at par with MDFH-3 × 3 m. The horti-silviculture system (MDFH-3 × 3 m) consistently produced the highest fruit yield throughout the years surpassing other treatments. This treatment increased the average dragon fruit yield by 115.3% compared to sole dragon fruit without HM. Hence, the adoption of the horti-silviculture system (MDFH-3 × 3 m) could be a promising strategy for achieving enhanced environmental and economic benefits in ravine lands. Therefore, dragon fruit based horti-silviculture system (MDFH-3 × 3 m) could be recommended for restoration of ravine lands, improving land productivity, and mitigating impact of soil erosion particularly in Western India or similar agro-climatic regions of the world.

First Report of Neoscytalidium dimidiatum Causing Stem Canker of Dragon fruit (Hylocereus spp.) in India.

Dragon fruit (Hylocereus spp.) is a tropical perennial plant (Family Cactaceae) that is popular for consumption in India. Originated from Central and South America and currently became popular in India. Hylocereus undatus and H. polyrhizus are the most commonly cultivated species in India. During August to December, 2021, stem cankers were observed in commercial orchards within the Satara, Pune and Solapur districts of Maharashtra. The disease incidence across four orchards was approx. 40% and severity ranged between 30 and 80%. Initial symptoms on infected cladodes were minute, circular, depressed chlorotic spots often with a brick red flecks followed by elevation of the centre of the lesion. Later the lesions turned necrotic and contained black, erumpent pycnidia, followed by chlorosis and stem rot. Twelve diseased cladodes from different orchards were collected and isolations were conducted. Edges of the lesions (5 to10 mm2) were excised and surface sterilized by exposure to 1% sodium hypochlorite (2 min) followed by triple rinsing with sterilized distilled water. Excess moisture was removed with sterilized blotter paper and pieces were plated on potato dextrose agar (PDA) amended with streptomycin sulphate (30 mg/L) for 3 days at 27 ± 2°C with a 12 h photoperiod. All twelve isolation attempts yielded uniform fungal colonies and out of these, purified colonies from each location viz., SLNeo, LNeo, MGNeo and KNeo were selected for etiology study. On PDA, initially white mycelial colonies turned to olive green to grayish with dark gray to black pigmentation. Colony growth was rapid (30 mm/day). Brown, branched septate hyphae fragmented to produce abundant arthroconidia in chains that were hyaline to dark brown, thick walled, 0 to 1 septate, ellipsoid to ovoid (10.1 ± 1.4 × 4.88 ± 1.1 µm), rod (9.0 ± 0.8 µm × 5.3 ± 0.8 µm), round (7.9 ± 1.6 µm) and capsule (14.2 ± 3.1 × 6.1 ± 0.8 µm) shape. Conidia from pycnidia developed on host tissues were aseptate, hyaline and ellipsoid-cylindrical (8.2 ± 0.8 × 2.2 ± 0.3 µm) in shape. Based on morphological characteristics, the isolates were identified as Neoscytalidium dimidiatum (Penz.) Crous & Slippers (Crous et al. 2006). Molecular characterization was done by amplifying the partial internal transcribed spacer (ITS) region, and translation elongation factor 1-α and ß-tubulin genes of four isolates using ITS1/ITS4, EF1-728F/EF1-986R and BT2A/BT2B primers (White et al. 1990, Glass and Donaldson 1995, Carbone and Kohn 1999). Sequences were deposited in GenBank accesions (ITS: OM884028, OM884029, OM884030, OM899800; TUB2: OM927962, OM927963, ON099066; TEF1-α: OM927965, OM927966, OM927964, OM984744) showed 99 to 100% identity with the epitype CBS 499.66 accession numbers KF531820 for ITS and KF531798 for TEF1-α and KF531800 for TUB2. N. dimidiatum isolate designated as SLNeo has been deposited at Microbial Type Culture Collection (MTCC), Chandigarh, India with MTCC 13250. Phylogenetic analysis using maximum likelihood (ML) revealed these isolates were clustered with N. dimidiatum clade. To evaluate pathogenicity, stems of one year old dragon fruit plants were wounded with a sterilized needle and inoculated with 7 mm fresh mycelial discs. For each isolate three replications were kept. Non-inoculated controls were plants receiving only non-colonized PDA discs. Inoculated and control plants were kept separately at ambient temperature (35 ± 2°C during day and 22 ± 2°C at night). Symptom started to develop at 2 days after inoculation (DAI) and canker lesions with chlorotic halos and rotting were observed at 15 DAI. No symptoms were observed on the negative control. Pathogenicity testing was repeated twice and the pathogen was re-isolated from symptomatic tissues with 100% re-isolation frequency and found identical with N. dimidiatum. N. dimidiatum has been reported on H. undatus and H. polyrhizus in subtropical and tropical countries worldwide (Chuang et al. 2012; Ezra et al. 2013; Lan et al. 2012; Sanahuja et al. 2016; Serrato-Diaz and Goenaga 2021). To our knowledge, this is the first report of dragon fruit stem canker caused by N. dimidiatum from India. Since the disease poses a major threat to dragon fruit plantations, additional epidemiological studies may assist in developing management strategies.

First report of Colletotrichum truncatum causing anthracnose of dragon fruit (Hylocereus spp.) in India.

Dragon fruit (Hylocereus spp.) is gaining popularity due to high net return, medicinal importance and ability to survive under less water and poor quality soils. In year 2020 and 2021, H. undatus and H. polyrhizus plants at research field of ICAR-National Institute of Abiotic Stress Management, Baramati (18°09'30.62″N, 74°30'08″E) were affected with anthracnose disease. Out of 340 plants, 60 were symptomatic, showed disease severity up to 25 to 30%. Intermittent raining in July to September and untimely rain in November, 2021 favored the disease. Infected cladodes showed reddish to dark-brown, sunken lesions, with chlorotic haloes later converted to mature necrotic patches with prominent black acervuli. On fruits, small, light brown spots quickly turned to sunken water-soaked lesions with concentric rings of black acervuli. Infected stems were collected randomly from different plants. For pathogen isolation, lesion edge tissues (5 to 10 mm2) were excised and disinfected with 1% Sodium hypochlorite (2 min) followed by triple rinsed with sterilized water and plated on potato dextrose agar (PDA) amended with Streptomycin sulphate (30 mg/L) for 4 days at 27 ± 2°C with a 12 h photoperiod. Purified colonies of three isolates 2CT, 6CT, D6CT were obtained from successive isolation attempts. Colonies were round with smooth margins, initially pale white mycelia that changed to dark gray with pinkish-orange conidial masses. Average colony diameter was 58.3 mm at 7 DAI. Conidia were single-celled, hyaline, slightly curved, tapered tip and truncate base, with an oil globule at center. Average conidia size (n=50) was 25.7 (±2.3) µm × 3.7 (±0.2) µm, L/W ratio=6.9. Conidia were initiated from an acervular conidiomata with intermittent dark brown, septate, straight, pointed setae 114 (±35) µm long × 4.5 (±1.1) µm wide. Appressoria were dark brown, lobate or round, mostly in groups, measuring 11 (±2.4) × 6.6 (±0.8) µm. Morphological characters were consistent with Colletotrichum truncatum (Schwein.) Andrus & W.D. Moore (Damm et al. 2009). For molecular identity of three isolates, partial internal transcribed spacer (ITS) region, actin, ß-tubulin (TUB2) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were amplified with ITS1/ITS4, ACT512F/ACT783R, BT2A/BT2B, and GDF1/GDR1 primers, respectively. Sequences were deposited in GenBank (ITS: OK639098 to OK639100; Actin: OM927967, OM927968, ON099061; TUB2: OM927969, ON099062, ON099063; GAPDH: ON099064, ON099065). A maximum likelihood phylogenetic tree based on all sequenced loci in MEGA11 shows the clustering of present isolates in the C. truncatum clade. For pathogenicity, 4 month old unwounded stems of H. undatus and H. polyrhizus were inoculated with a spore spray (1x106/ml conidia) of C. truncatum. For each isolate, three plants were inoculated. Plants inoculated with sterilized water represented the negative control. Inoculated and control plants were kept separately at 25 ± 2°C temperature and >85% relative humidity. Inoculated plants showed minute, sunken, water soaked, reddish brown spots which were converted to sunken patches with black acervuli at 15 DAI. No symptoms were observed in the negative control. Pathogenicity test was repeated twice and the pathogen was re-isolated from symptomatic stems showed similar morphology with C. truncatum. Based on morphological and molecular characteristics and pathogenicity test, pathogen identified as C. truncatum. Previously, dragon fruit anthracnose caused by C. truncatum was reported from China (Guo et al. 2014) and Malaysia (Vijaya et al. 2015). To our knowledge, this is the first report of C. truncatum cause of dragon fruit anthracnose in India. Detailed pathogen diagnostics may help in formulating effective, on time, appropriate disease management strategies.

Agroforestry for controlling soil erosion and enhancing system productivity in ravine lands of Western India under climate change scenario.

Soil erosion in semi-arid climate leading to the development of ravine lands is the most severe form of land degradation. Ravine lands are formed when soil is not fully covered by the vegetation throughout the year and sporadic vegetation is not able to bind the soil particles from being washed away by rainfall. Throughout the globe, ravine lands have severe limitations for their rehabilitation and sustainable utilization as a consequence of its unique topographical features. Climatic and edaphic stresses make crop production extremely challenging in these lands. Practicing sole cropping promotes erosion, produces low crop yield, utilizes high energy, and emits greenhouse gasses (GHGs). Tree cultivation either sole or in combination with crops (agroforestry) has a strong potential to control erosion, produce sustainable economic yield, reduce energy consumption, and sequester greater amount of atmospheric carbon dioxide in biomass and soil carbon pools besides providing various ecosystem services. Therefore, practicing agroforestry could be a promising approach to obtain the greater environmental and economic benefits in the ravine lands. The present study was conducted on three systems, i.e., sole crop cultivation (cowpea + castor), agroforestry (sapota + cowpea + castor), and sole sapota plantation, to evaluate their impact on soil erosion, runoff, system productivity, profitability, energetics, and carbon sequestration during the 4-year period (2017-2020). The results revealed that agroforestry reduced the total soil loss and runoff by 37.7% and 19.1%, respectively, compared to the sole crop cultivation. Likewise, the highest system productivity as cowpea equivalent yield (CEY) was obtained under agroforestry system that increased the CEY by 162% and 81.9%, compared to sole crop and sole tree plantation, respectively. The climate change mitigation potential in terms of net carbon balance was observed highest in sole tree plantation (8.4 t/ha) followed by agroforestry system (5.9 t/ha) and lowest in sole cropping system (-2.8 t/ha). Therefore, an agroforestry system could be recommended for controlling soil erosion, improving system productivity and profitability, and reducing energy consumption as well as mitigating climate change in ravine lands.