Effects of sodium bicarbonate concentration on growth, photosynthesis, and carbonic anhydrase activity of macroalgae Gracilariopsis lemaneiformis, Gracilaria vermiculophylla, and Gracilaria chouae (Gracilariales, Rhodophyta).

There is potential for bicarbonate to improve crop yields and economic efficiency of marine algae. However, few studies have focused on the effect of bicarbonate on the growth, photosynthesis, and enzyme activity associated with carbon utilization, especially in commercial macroalgae. Here, the addition of bicarbonate (up to 420 mg L(-1)) to macroalgal cultures has been evaluated for Gracilariopsis lemaneiformis, Gracilaria vermiculophylla, and Gracilaria chouae with respect to growth rate, photosynthetic activity, carbonic anhydrase activity, and biochemical composition. The results showed that the effects of NaHCO3 on growth, chlorophyll a, phycoerythrin, photosynthetic oxygen evolution, photochemical parameters of PSI and PSII, carbonic anhydrase activity, and nitrogen content were significant (P < 0.05) and followed the same pattern in the three species. The parameter values were promoted in lower NaHCO3 concentrations (up to 252 or 336 mg L(-1)) and inhibited in higher NaHCO3 concentrations (>336 mg L(-1) for Gp. lemaneiformis and >420 mg L(-1) for the other two species). Moreover, species-specific differences induced by supplementation with bicarbonate were discovered during culture. Optimal concentrations of NaHCO3 used in this study were 252 mg L(-1) for Gp. lemaneiformis and 336 mg L(-1) for G. vermiculophylla and G. chouae. These results suggest that an adequate supplementation of sodium bicarbonate is a viable strategy for promoting growth and photosynthetic activity in some macroalgae as well as for improving biochemical composition. The study will help to accelerate the growth rate of algae and improve the quality of thalli, and will also be useful for enhancing the understanding of carbon utilization in macroalgae.

Clear speech production of Korean stops: changing phonetic targets and enhancement strategies.

The proposal that phonological contrast is enhanced through greater approximation of phonetic targets was investigated by comparing clear speech to conversational and citation-form speech produced by Korean speakers. The stop system of Korean is undergoing a sound change in which younger speakers produce the aspirated-lenis stop contrast differently from previous generations. Older speakers differentiate this contrast primarily with the acoustic correlate of voice onset time (VOT) and secondarily with F0. Younger speakers are merging VOT values for this contrast. As a result, the primary acoustic correlate is now F0 for younger speakers. These production differences likely indicate that younger speakers have developed different phonetic targets for stop production. These different phonetic targets were predicted to result in different enhancement patterns in clear speech: Younger speakers were predicted to enhance F0 differences, whereas older speakers were predicted to enhance VOT differences in clear speech. Results indicated that the older group solely used VOT to enhance the contrast in clear speech, whereas the younger group primarily used F0 but also demonstrated small VOT enhancement. These results indicate that clear speech enhancement strategies reflect phonetic targets. Older and younger speakers have different F0 and VOT targets and these different targets conditioned different enhancement strategies.

Response of sulfide:quinone oxidoreductase to sulfide exposure in the echiuran worm Urechis unicinctus.

Sulfide is a natural, widely distributed, poisonous substance, and sulfide:quinone oxidoreductase (SQR) is responsible for the initial oxidation of sulfide in mitochondria. In this study, we examined the response of SQR to sulfide exposure (25, 50, and 150 µM) at mRNA, protein, and enzyme activity levels in the body wall and hindgut of the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. The results revealed SQR mRNA expression during sulfide exposure in the body wall and hindgut increased in a time- and concentration-dependent manner that increased significantly at 12 h and continuously increased with time. At the protein level, SQR expression in the two tissues showed a time-dependent relationship that increased significantly at 12 h in 50 µM sulfide and 6 h in 150 µM, and then continued to increase with time while no significant increase appeared after 25 µM sulfide exposure. SQR enzyme activity in both tissues increased significantly in a time-dependent manner after 50 µM sulfide exposure. We concluded that SQR expression could be induced by sulfide exposure and that the two tissues studied have dissimilar sulfide metabolic patterns. A U. unicinctus sulfide-induced detoxification mechanism was also discussed.

Sulfide:quinone oxidoreductase from echiuran worm Urechis unicinctus.

Sulfide is a natural, widely distributed, poisonous substance, and sulfide:quinone oxidoreductase (SQR) has been identified to be responsible for the initial oxidation of sulfide in mitochondria. In this study, full-length SQR cDNA was cloned from the echiuran worm Urechis unicinctus, a benthic organism living in marine sediments. The protein consisted of 451 amino acids with a theoretical pI of 8.98 and molecular weight of 50.5 kDa. Subsequently, the SQR mRNA expression in different tissues was assessed by real-time reverse transcription and polymerase chain reaction and showed that the highest expression was in midgut, followed by anal sacs and coelomic fluid cells, and then body wall and hindgut. Furthermore, activated SQR was obtained by dilution refolding of recombinant SQR expression in E. coli, and the refolded product showed optimal activity at 37 °C and pH 8.5 and K (m) for ubiquinone and sulfide at 15.6 µM and 40.3 µM, respectively. EDTA and GSH had an activating effect on refolded SQR, while Zn(2+) caused decreased activity. Western blot showed that SQR in vivo was located in mitochondria and was ∼ 10 kDa heavier than the recombinant protein. In addition, SQR, detected by immunohistochemistry, was mainly located in the epithelium of all tissues examined. Ultrastructural observations of these tissues' epithelium by transmission electron microscopy provided indirect cytological evidence for its mitochondrial location. Interesting aspects of the U. unicinctus SQR amino acid sequence, its catalytic mechanism, and the different roles of these tissues in sulfide metabolic adaptation are also discussed.

Phonological systems in bilinguals: age of learning effects on the stop consonant systems of Korean-English bilinguals.

Interaction of Korean and English stop systems in Korean-English bilinguals as a function of age of acquisition (AOA) of English was investigated. It was hypothesized that early bilinguals (mean AOA=3.8 years) would more likely be native-like in production of English and Korean stops and maintain greater independence between Korean and English stop systems than late bilinguals (mean AOA=21.4 years). Production of Korean and English stops was analyzed in terms of three acoustic-phonetic properties: voice-onset time, amplitude difference between the first two harmonics, and fundamental frequency. Late bilinguals were different from English monolinguals for English voiceless and voiced stops in all three properties. As for Korean stops, late bilinguals were different from Korean monolinguals for fortis stops in voice-onset time. Early bilinguals were not different from the monolinguals of either language. Considering the independence of the two stop systems, late bilinguals seem to have merged English voiceless and Korean aspirated stops and produced English voiced stops with similarities to both Korean fortis and lenis stops, whereas early bilinguals produced five distinct stop types. Thus, the early bilinguals seem to have two independent stop systems, whereas the late bilinguals likely have a merged Korean-English system.