Targeted association analysis identified japonica rice varieties achieving Na(+)/K (+) homeostasis without the allelic make-up of the salt tolerant indica variety Nona Bokra.

During the last decade, a large number of QTLs and candidate genes for rice tolerance to salinity have been reported. Using 124 SNP and 52 SSR markers, we targeted 14 QTLs and 65 candidate genes for association mapping within the European Rice Core collection (ERCC) comprising 180 japonica accessions. Significant differences in phenotypic response to salinity were observed. Nineteen distinct loci significantly associated with one or more phenotypic response traits were detected. Linkage disequilibrium between these loci was extremely low, indicating a random distribution of favourable alleles in the ERCC. Analysis of the function of these loci indicated that all major tolerance mechanisms were present in the ERCC although the useful level of expression of the different mechanisms was scattered among different accessions. Under moderate salinity stress some accessions achieved the same level of control of Na(+) concentration and Na(+)/K(+) equilibrium as the indica reference variety for salinity tolerance Nona Bokra, although without sharing the same alleles at several loci associated with Na(+) concentration. This suggests (a) differences between indica and japonica subspecies in the effect of QTLs and genes involved in salinity tolerance and (b) further potential for the improvement of tolerance to salinity above the tolerance level of Nona Bokra, provided the underlying mechanisms are complementary at the whole plant level. No accession carried all favourable alleles, or showed the best phenotypic responses for all traits measured. At least nine accessions were needed to assemble the favourable alleles and all the best phenotypic responses. An effective strategy for the accumulation of the favourable alleles would be marker-assisted population improvement.

NATURAL RADIOACTIVITY STUDIES OF PHOSPHATE FERTILIZERS APPLIED ON GREEK FARM SOILS USED FOR WHEAT CULTIVATION.

Naturally Occurring Radionuclide Materials (NORM) such as fertilizers, agricultural soils fertilized and wheat grain cultivated at those soils were studied regarding the radiation hazard to the public and workers. Activity of 238U, 226Ra, 232Th and 40K was measured hermitical sealing the marinelli beaker using the Stopaq FN 2100L material that prevent 222Rn escape from the baker. The phosphate fertilization of soil did not affect natural radioactivity in comparison with non-fertilized soils and also did not affect natural radioactivity of wheat grain cultivated since only 40K was measured. The high mean value of Dair 211 nGy h-1 for fertilizers compared to soils fertilized 53 nGy h-1 highlights the raising concern about radioprotection of workers occupied with tasks involving fertilizers. The maximum external dose rate 1.15 mSv y-1 calculated for truck drivers involved to transportation section is similar to annual external effective dose limit 1 mSv. Real concerns rise for workers in the fertilizers storage and supply department since the maximum dose rate estimated is 1.98 mSv y-1, while the mean value is 0.89 mSv y-1.

Laboratory and field dissipation of penoxsulam, tricyclazole and profoxydim in rice paddy systems.

Rice cultivation relies on pesticide applications to ensure high yields. However, the regular use of pesticides seriously affects the quality of neighboring surface water systems. Thus complete knowledge of the environmental fate and dissipation of pesticides in the paddy rice environment should become available. So far only a few studies have provided comprehensive assessment of the dissipation of pesticides under the submerged cultivation conditions followed in rice. Thus, laboratory and 2-year field studies were performed to assess the dissipation of two new generation rice herbicides (penoxsulam and profoxydim) and one of the most important rice fungicides (tricyclazole). A good agreement between laboratory and field experiments was observed with a faster dissipation of penoxsulam and tricyclazole under field conditions. Profoxydim was the least persistent chemical (DT50 soil<1d; DT50 water 0.5-1.2d), followed by penoxsulam which persisted for longer particularly in the water compartment (DT50water=3.8-5.9d). Tricyclazole was the most persistent pesticide, especially in the soil compartment with DT50 values of 44.5-84.6 (field) and 197d (laboratory). These results could be utilized for the assessment of the environmental risk associated with the use of those pesticides in rice cultivation and the determination of potential mitigation measures for minimizing the risk for contamination of neighboring natural water resources.