Influence of planting dates and fertilizer modules on yield of chrysanthemum and soil health.

BACKGROUND: Optimum planting date and appropriate fertilizer module are essential facets of chrysanthemum cultivation, to enhance quality yield, and improve soil health. A field-based study was undertaken over multiple growing seasons in 2022 and 2023, where six different planting dates, viz., P1:June 15, P2:June 30, P3:July 15, P4:July 30, P5:August 15 and P6:August 30 and two fertilizer modules, FM1:Jeevamrit @ 30 ml plant-1 and FM2:NPK @ 30 g m-2 were systematically examined using a Randomized Block Design (factorial), replicated thrice. RESULTS: P6 planting resulted in early bud formation (44.03 days) and harvesting stage (90.78 days). Maximum plant height (79.44 cm), plant spread (34.04 cm), cut stem length (68.40 cm), flower diameter (7.83 cm), stem strength (19.38˚), vase life (14.90 days), flowering duration (24.08 days), available soil N (314 kg ha-1), available P (37 kg ha-1), available K (347 kg ha-1), bacterial count (124.87 × 107 cfu g-1 soil), actinomycetes count (60.72 × 102 cfu g-1 soil), fungal count (30.95 × 102 cfu g-1 soil), microbial biomass (48.79 µg g-1 soil), dehydrogenase enzyme (3.64 mg TPF h-1 g-1 soil) and phosphatase enzyme (23.79 mol PNP h-1 g-1 soil) was recorded in P1 planting. Among the fertilization module, minimum days to bud formation (74.94 days) and days to reach the harvesting stage (120.95 days) were recorded with the application of NPK @30 g m-2. However, maximum plant height (60.62 cm), plant spread (23.10 cm), number of cut stems m-2 (43.88), cut stem length (51.34 cm), flower diameter (6.92 cm), stem strength (21.24˚), flowering duration (21.75 days), available soil N (317 kg ha-1), available P (37 kg ha-1) and available K (349 kg ha-1) were also recorded with the application of NPK @300 kg ha-1. Maximum vase life (13.87 days), OC (1.13%), bacterial count (131.65 × 107 cfu g-1 soil), actinomycetes count (60.89 × 102 cfu g-1 soil), fungal count (31.11 × 102 cfu g-1 soil), microbial biomass (51.27 µg g-1 soil), dehydrogenase enzyme (3.77 mg TPF h-1 g-1 soil) and phosphatase enzyme (21.72 mol PNP h-1 g-1 soil) were observed with the application of Jeevamrit @ 30 ml plant-1. CONCLUSION: Early planting (P1) and inorganic fertilization (NPK @ 30 g m-2) resulted in improved yield and soil macronutrient content. The soil microbial population and enzymatic activity were improved with the jeevamrit application. This approach highlights the potential for improved yield and soil health in chrysanthemum cultivation, promoting a more eco-friendly and economically viable agricultural model.

Enhancing productivity and sustainability of ravine lands through horti-silviculture and soil moisture conservation: A pathway to land degradation neutrality.

Ravine lands are the worst type of land degradation affecting soil quality and biodiversity. Crop production in such lands is impossible without adopting proper conservation measures. In-situ moisture conservation techniques could play an instrumental role in restoring ravine lands by improving soil moisture. We hypothesized that restoring ravine land through a combination of tree planting, fruit crop cultivation, and in-situ moisture conservation practice would result in significant improvements in productivity, profitability, and soil fertility. An experiment was conducted involving the combination of Malabar Neem (Melia dubia) and Dragon fruit (Hylocereus undatus) in conjunction with in-situ soil moisture conservation measures specifically involving half-moon structures (HM). The experiment was conducted under randomized block design (RBD) comprising eight treatments. These treatments include sole Melia cultivation (MD 3m × 3m), sole cultivation of dragon fruit (DF 3m × 3m), silviculture system (MDF-3m × 3m), horti-silviculture system with larger spacing (MDF-4m × 4m), sole Melia cultivation with in-situ moisture conservation (MDH-3m × 3m), sole Dragon fruit cultivation with in-situ moisture conservation (DFH-3m × 3m), horti-silviculture system of Melia and Dragon fruit with in-situ moisture conservation (MDFH-3m × 3m), and horti-silviculture system with larger spacing and in-situ moisture conservation (MDFH-4m × 4m). Each treatment was replicated thrice to evaluate their impact on productivity, profitability, soil fertility, and carbon sequestration for 8 years (2016-2023). The results revealed that the horti-silviculture system (MDFH-3 × 3 m) exhibited the highest total tree biomass and total carbon sequestration with an increase of 183.2% and 82.8% respectively, compared to sole Melia cultivation without HM and sole Melia with HM. Furthermore, sole Melia with HM augmented soil nutrients (N, P, K, and SOC) by 74.4%, 66.4%, 35.2%, and 78.3%, respectively, compared to control (no planting), with performance at par with MDFH-3 × 3 m. Similarly, sole Melia with HM enhanced SOC stock and SOC sequestration rate by 79.2% and 248% over control. However, it was found at par with MDFH-3 × 3 m. The horti-silviculture system (MDFH-3 × 3 m) consistently produced the highest fruit yield throughout the years surpassing other treatments. This treatment increased the average dragon fruit yield by 115.3% compared to sole dragon fruit without HM. Hence, the adoption of the horti-silviculture system (MDFH-3 × 3 m) could be a promising strategy for achieving enhanced environmental and economic benefits in ravine lands. Therefore, dragon fruit based horti-silviculture system (MDFH-3 × 3 m) could be recommended for restoration of ravine lands, improving land productivity, and mitigating impact of soil erosion particularly in Western India or similar agro-climatic regions of the world.

System and Method for Driver Drowsiness Detection Using Behavioral and Sensor-Based Physiological Measures.

The amount of road accidents caused by driver drowsiness is one of the world's major challenges. These accidents lead to numerous fatal and non-fatal injuries which impose substantial financial strain on individuals and governments every year. As a result, it is critical to prevent catastrophic accidents and reduce the financial burden on society caused by driver drowsiness. The research community has primarily focused on two approaches to identify driver drowsiness during the last decade: intrusive and non-intrusive. The intrusive approach includes physiological measures, and the non-intrusive approach includes vehicle-based and behavioral measures. In an intrusive approach, sensors are used to detect driver drowsiness by placing them on the driver's body, whereas in a non-intrusive approach, a camera is used for drowsiness detection by identifying yawning patterns, eyelid movement and head inclination. Noticeably, most research has been conducted in driver drowsiness detection methods using only single measures that failed to produce good outcomes. Furthermore, these measures were only functional in certain conditions. This paper proposes a model that combines the two approaches, non-intrusive and intrusive, to detect driver drowsiness. Behavioral measures as a non-intrusive approach and sensor-based physiological measures as an intrusive approach are combined to detect driver drowsiness. The proposed hybrid model uses AI-based Multi-Task Cascaded Convolutional Neural Networks (MTCNN) as a behavioral measure to recognize the driver's facial features, and the Galvanic Skin Response (GSR) sensor as a physiological measure to collect the skin conductance of the driver that helps to increase the overall accuracy. Furthermore, the model's efficacy has been computed in a simulated environment. The outcome shows that the proposed hybrid model is capable of identifying the transition from awake to a drowsy state in the driver in all conditions with the efficacy of 91%.

Enhanced antimicrobial and antioxidant properties of Nano chitosan and pectin based biodegradable active packaging films incorporated with fennel (Foeniculum vulgare) essential oil and potato (Solanum tuberosum) peel extracts.

Active packaging materials are generally prepared by incorporating antimicrobial agents. The main objective of the present research is the development and characterization of active packaging with Nano chitosan and pectin. Active packaging film was prepared by incorporating essential oil of Fennel (EOF) along with potato peel extract (PPE) to improve the antioxidant and antimicrobial property of the film. Incorporation of EOF into Nano chitosan and pectin-based films increased antimicrobial activity, whereas; PPE increased the antioxidant property of the films. In the present study, thickness, optical property, mechanical property, antioxidant and antimicrobial property, total soluble matter, morphological study, FTIR analysis, and microbial degradation study of the prepared active packaging material was done. The addition of the PPE and EOF together improved the surface, optical, mechanical, antimicrobial and antioxidant properties of the packaging film. Enhanced antimicrobial and antioxidant property of the Nano chitosan-PPE-PVA-EOF and pectin-PPE-PVA-EOF-based packaging films can extend the shelf life of the packed food; hence it is suggested for the active packaging for perishable food commodity. In short, the prepared active packaging film with biodegradable property is suggested as an effective packaging material to replace synthetic plastic packages for food and hence reduce plastic pollution. Supplementary Information: The online version contains supplementary material available at 10.1007/s13197-021-05333-9.

Distribution of soil carbon fractions under different bamboo species in northwest Himalayan foothills, India.

Soil carbon and its fractions are important in understanding the mechanism of soil carbon sequestration. The present study evaluated the impact of seven commercial bamboo species, viz., Bambusa balcooa, B. bambos, B. vulgaris, B. nutans, Dendrocalamus hamiltonii, D. stocksii, and D. strictus, on labile and non-labile carbon fractions. In the 0-15-cm layer, B. nutans had the highest very labile C (7.65 g kg-1) followed by B. vulgaris > B. balcooa > D. stocksii > D. hamiltonii > B. bambos > D. strictus > open. The active carbon pool was significantly low under the control plot (i.e. the open) indicating the positive influence of bamboo in soil C build-up in the top 0-15 cm soil layer. Amongst the different species of bamboo evaluated in this study, D. strictus accumulated the highest active C pool in 0-30-cm soil layer followed by B. vulgaris. Of the total organic C in the 0-30 cm soil depth, majority (55-60%) was contributed by the passive C pool comprising the less labile and the non-labile fraction of SOC. A high value of carbon stratification ratio (> 2) was observed for D. strictus, B. bambos, and D. hamiltonii which proves their potential for restoration of the degraded lands. The majority of bamboo species except for B. balcooa and D. stocksii showed a higher carbon management index than open systems, thereby indicating higher rates of soil C rehabilitation. Of the seven bamboo species, B. vulgaris, D. strictus, and B. nutans can be adopted for cultivation in the northwest Himalayas given their ability to positively impact the SOC and its fractions in both surface and sub-surface soil.

Assessment of eco-hydrological parameters for important sympodial bamboo species in Himalayan foothills.

Bamboos due to high soil water conservation potential are gaining increased attention in plantation programs across the globe. Large-scale plantation of fast-growing bamboo, however, can have important hydrological consequences. The study aims to quantify the eco-hydrological parameters, viz., throughfall (TF), stemflow (SF), and interception (I) in seven important sympodial bamboo species in north western Himalayan foothills of India. The species selected include Bambusa balcooa, Bambusa bambos, Bambusa vulgaris., Bambusa nutans, Dendrocalamus hamiltonii, Dendrocalamus stocksii, and Dendrocalamus strictus. Throughfall versus gross rainfall (GR) relationship in different species indicated high throughfall production during high rainfall events with r2 > 0.90. Average throughfall was lowest (62.1%) in D. hamiltonii and highest in B. vulgaris (74.6%). SF ranged from 1.32% in B. nutans to 3.39% in D. hamiltonii. The correlation coefficient (r) between leaf area index (LAI), number of culms, and crown area with the interception were 0.746, 0.691, and 0.585, respectively. The funneling ratio (F) was highest (27.0) in D. hamiltonii and least in B. nutans. Canopy storage capacity was highest in D. strictus (3.57 mm) and least in D. hamiltonii (1.09 mm). Interception loss was highest (34.4%) in D. hamiltonii and lowest in B. vulgaris (23.5%) and D. strictus (23.6%). Higher interception in bamboos make them suitable for soil conservation, but careful selection of species is required in low rainfall areas.

Assessment of urban growth effects on green space and surface temperature in Doon Valley, Uttarakhand, India.

This study aims to examine the spatio-temporal urban expansion pattern and its impacts on green space variation as well as thermal behavior in Doon valley over the last two decades during 2000 and 2019. Landsat 5 and Landsat 8 images of February and May month of two study years 2000 and 2019 were used for the analysis. The land use change analysis revealed notable outgrowth of urbanization with 184% increase in Doon valley during 2000-2019. To examine the effects of locational factors on urban growth, relative Shannon entropy analysis was carried out based on two factors, i.e., distances from city center and roads. It was seen that all the roads and city center have witnessed consistent and higher urban spread in its surroundings with high relative entropy value more than 0.9. Further analysis shows that there was considerable loss of agriculture crop lands and fallow lands along the major roads and around city center. Forest area was mostly affected along the road towards Mussorie hill station (road 2) because of its hilly surroundings whereas in Subhash Nagar area (road 4), fallow land and cultivated land were mainly replaced by the development activities. Analysis was also carried out to assess the spatial-temporal distribution of land surface temperature (LST) and its changing dynamics with land covers. It revealed that LST has increased in all the land use types with overall increase of 1.86 °C and 8.62 °C in the months February and May, respectively, during the study period. It is also found that normalized difference vegetation index (NDVI) and LST are negatively correlated with R2 0.46 and 0.28 for the months February and May, respectively. However, the correlation between NDVI and LST was found highly significant with P value less than 0.01. Therefore, spatial and temporal changes of different land use types especially rapid urbanization at the cost of green spaces with rampant anthropogenic activities is one of the main factor for LST increase in the study area. Moreover, this temperature rise with ever-increasing anthropogenic activities is not a healthy indication for the hilly region like Doon Valley which may adversely affect the ecosystem stability and its resources as well. The study may be used as reference for future ecological and urban management studies and policies.

Microbial chitinases and their relevance in various industries.

Chitin, the second most abundant biopolymer on earth after cellulose, is composed of ß-1,4-N-acetylglucosamine (GlcNAc) units. It is widely distributed in nature, especially as a structural polysaccharide in the cell walls of fungi, the exoskeletons of crustaceans, insects, and nematodes. However, the principal commercial source of chitin is the shells of marine or freshwater invertebrates. Microbial chitinases are largely responsible for chitin breakdown in nature, and they play an important role in the ecosystem's carbon and nitrogen balance. Several microbial chitinases have been characterized and are gaining prominence for their applications in various sectors. The current review focuses on chitinases of microbial origin, their diversity, and their characteristics. The applications of chitinases in several industries such as agriculture, food, the environment, and pharmaceutical sectors are also highlighted.

Adaptive resilience of roadside trees to vehicular emissions via leaf enzymatic, physiological, and anatomical trait modulations.

Unplanned urbanization and heavy automobile use by the rapidly growing population contribute to a variety of environmental issues. Roadside plants can mitigate air pollution by modifying their enzymatic activity, physiological and anatomical traits. Plant enzymes, physiological and anatomical traits play an important role in adaptation and mitigation mechanisms against vehicular emissions. There is a significant gap in understanding of how plant enzymes and anatomical traits respond or how they participate in modulating the effect of vehicular emissions/air pollution. Modulation of leaf anatomical traits is also useful in regulating plant physiological behavior. Hence, the present study was conducted to evaluate the effects of vehicular pollution on the enzymatic activity, physiological, and anatomical traits of plant species that grow in forests (S1) and alongside roads (S2-1 km away from the S1 site) during different seasons. The present study examines four commonly found roadside tree species i.e. Grevillea robusta, Cassia fistula, Quercus leucotrichophora and Cornus oblonga. The study found that the activities of catalase and phenylalanine ammonium enzymes were higher in G. robusta species of roadside than control site (S1). Non-enzymatic antioxidants such as flavonoid and phenol were also found in higher concentrations in roadside tree species during the summer season. However, the measured values of physiological traits were higher in Q. leucotrichophora tree species of S1 during the summer season. When compared to the other species along the roadside, Q. leucotrichophora had the highest number of stomata and epidermal cells during the summer season. Hence, we found that tree species grown along the roadside adapted towards vehicular emissions by modulating their enzymatic, physiological, and anatomical traits to mitigate the effect of air pollution.

Build-up of labile, non-labile carbon fractions under fourteen-year-old bamboo plantations in the Himalayan foothills.

Carbon fractions under different bamboo species viz., Bambusa balcooa, Bambusa bambos, Bambusa nutans, Dendrocalamus hamiltonii, Dendrocalamus asper and Dendrocalamus strictus were evaluated to understand the potential of these different bamboo species in soil rehabilitation in Himalayan foothills. The highest accumulation of the different carbon fractions likes very labile (6.12 mg g-1), less labile (2.55 mg g-1) and non-labile (11.40 mg g-1) was observed under D. hamiltonii, while highest labile fraction (3.17 mg g-1) was recorded under D. strictus. The highest active (8.85 mg g-1) and passive pool (13.95 mg g-1) were recorded under D. hamiltonii. Higher carbon management index (CMI) was obtained under D. hamiltonii (186.04) which was comparable with D. strictus (182.66) and B. nutans (179.24). Among all the six species, D. hamiltonii had the highest buildup of active and passive pool in both the soil depths. Bamboo plantations irrespective of the different species helped in enhancing the SOC fraction and enhanced C buildup in the soil in comparison to the open fallow land and holds potential in combating the problems of land degradation and soil rehabilitation.

Field Based Assessment of Capsicum annuum Performance with Inoculation of Rhizobacterial Consortia.

Plant growth promoting rhizobacteria (PGPR) are associated with plant roots and augment plant productivity and immunity by reducing fertilizer application rates and nutrient runoff. Studies were conducted to evaluate bell pepper transplants amended with formulation of consortium of two indigenous PGPR isolates (Bacillus subtilis and Bacillus pumilus) in terms of increase in yield and disease resistance under field conditions. Transplants were planted into plots treated by NPK (nitrogen, phosphorus and potassium), fungicides, soil solarization, MeBr fumigation, PGPR and untreated soil. Treatments were assessed for incidence of soil-borne phytopathogens viz. Phytophthora capsici and Colletotrichum sp. Highly significant increases in bell pepper transplant growth occurred in response to formulations of PGPR isolates. Transplant vigor and survival in the field were also improved by PGPR treatments. Consortium of Bacillus subtilis and Bacillus pumilus reduced disease incidence of damping off by 1.81% and anthracnose by 1.75%. Numbers of colony forming units of Phytophthora capsici and Colletotrichum sp. were significantly higher in all plots than those treated with PGPR consortium. Incidence of seed rot and seedling blight on bell pepper was significantly lower in PGPR-treated plots and highest in untreated plots. Total fruit yield of bell pepper increased by 379.36% with PGPR consortium (Bacillus subtilis and Bacillus pumilus).

Preparation and characterization of CuO catalyst for the thermolysis treatment of distillery wastewater.

CuO catalyst was prepared from copper sulfate by alkali precipitation method followed by drying and calcination. Characterization of CuO catalyst using X-ray diffraction, Brunauer-Emmett-Teller, and Barrett-Joyner-Halenda surface area analysis envisaged the effectiveness of CuO as a catalyst for the treatment of biodigester effluent (BDE) emanated from distilleries. The catalytic thermolysis is an efficient advance treatment method for distillery biodigester effluent (BDE). CT treatment of BDE was carried out in a 0.5 dm3 thermolytic batch reactor using CuO as a catalyst at different pH (1-9), temperatures (80-110°C), and catalyst loadings (1-4 kg/m3). With CuO catalyst, a temperature of 110°C, catalyst loading of 4 kg/m3, and pH of 2 was found to be optimal, providing a maximum reduction in chemical oxygen demand of 65%. The settling characteristics at different temperatures of CT-treated sludge were also presented.

Bacillus pumilus strain YSPMK11 as plant growth promoter and bicontrol agent against Sclerotinia sclerotiorum.

A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35 °C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus. Furthermore, the genome of this isolate was searched for antimicrobial lipopeptide, i.e., ItuD and SrfC genes. The PCR amplification results showed the presence of both these lipopeptide genes in isolate YSPMK11. Iturin A as antifungal compound was identified as major components of fraction through GC/MS. In field experiments, the application of strain YSPMK11 cell suspension (108 CFU/ml) suppressed disease severity by 93% and increased curd yield by 36% which was more that of commercially used fungicide in farmer practices under mid-hills of Himachal Pradesh. Conclusively, our study is first to demonstrate the effect of B. pumilus strain YSPMK11 in suppression of SSR under field conditions and would be employed as an efficient biocontrol agent to replace commercial fungicides in cauliflower cropping system. In addition, the presence of both lipopeptide genes (ItuD and SrfC) and iturin A in this isolate makes him potent strain for biological control application in agriculture.

Regeneration complexities of Pinus gerardiana in dry temperate forests of Indian Himalaya.

Pinus gerardiana is considered an important species in dry temperate forests of North-Western Indian Himalaya because of its influence on ecological processes and economic dependence of local people in the region. But, large numbers of biotic and abiotic factors have affected P. gerardiana in these forests; hence, there is a crucial need to understand the regeneration dynamics of this tree species. The present investigation was conducted in P. gerardiana forests to understand vegetation pattern and regeneration processes on different sites in the region. Statistical analysis was performed to know variability in growing stock and regeneration on sample plots, while correlation coefficients and regression models were developed to find the relationship between regeneration and site factors. The vegetation study showed dominance of P. gerardiana, which is followed by Cedrus deodara, Pinus wallichiana and Quercus ilex in the region. The growing stock of P. gerardiana showed steep increasing and then steadily declining trend from lower to higher diameter class. The distribution of seedling, sapling, pole and trees was not uniform at different sites and less number of plots in each site were observed to have effective conditions for continuous regeneration, but mostly showed extremely limited regeneration. Regeneration success ranging from 8.44 to 15.93 % was recorded in different sites of the region, which suggests that in different sites regeneration success is influenced by collection of cone for extracting seed, grazing/browsing and physico-chemical properties of soil. Regeneration success showed significant correlation and relationship with most of abiotic and biotic factors. The regeneration success is lower than the requirement of sustainable forest, but varies widely among sites in dry temperate forests of Himalaya. More forest surveys are required to understand the conditions necessary for greater success of P. gerardiana in the region.

Maintenance pemetrexed in nonsmall cell lung carcinoma: Outcome analysis from a tertiary care center.

BACKGROUND: Maintenance pemetrexed is a standard treatment option for selected non squamous nonsmall cell lung carcinoma patients having a response to platin based doublet. We conducted a clinical audit of such selected patients and report the outcome among the Indian population. AIM: To evaluate the outcomes with maintenance pemetrexed in the patients with locally advanced and metastatic adenocarcinoma lung. OBJECTIVES: To calculate the progression free survival (PFS), overall survival (OS), and factors affecting the outcome. MATERIALS AND METHODS: Data of patients with locally advanced and metastatic adenocarcinoma lung were retrieved from prospectively maintained lung cancer database registered between June 2011 and March 2014. The patients who achieved partial response (n = 87) or stable disease (n = 101) after 6 cycles of pemetrexed platin based doublet and received the maintenance pemetrexed were selected for final analysis (n = 188). Kaplan-Meir survival analysis was used for PFS and OS. Log rank test was used to evaluate the factors affecting the outcome. RESULTS: Median follow-up is 14 months. The median number of maintenance pemetrexed cycles received is 6 (1-38). Common reason for the discontinuation are disease progression (n = 127), renal toxicity (n = 4), and social/financial (n = 7). Median PFS and OS are 8 months and 20 months, respectively. The patients with baseline pleural effusion had better PFS (9 months vs. 7 months, P = 0.02) and OS (26 months vs. 18 months, P = 0.05). The patients receiving more than 6 cycles of maintenance had improved PFS (12 vs. 7 months, P = 0.002) and OS (26 vs. 16 months, P = 0.05). CONCLUSION: Maintenance pemetrexed is feasible and well tolerated by the majority of Indian patients who achieved the response after platin based doublet. The patients with baseline pleural effusion benefit more with maintenance pemetrexed.