RESUMO
The case of a patient with immune thrombocytopenia associated with mycoplasma pneumoniae infection is described. The thrombocytopenia was considered to be provoked by the infection and disappeared gradually after antibiotic treatment.
Assuntos
Pneumonia por Mycoplasma/complicações , Púrpura Trombocitopênica/etiologia , Adolescente , Eritromicina/uso terapêutico , Humanos , Masculino , Pneumonia por Mycoplasma/diagnóstico , Pneumonia por Mycoplasma/tratamento farmacológico , Prednisona/uso terapêutico , Púrpura Trombocitopênica/tratamento farmacológico , Púrpura Trombocitopênica/imunologiaRESUMO
A middle-aged female with chronic diarrhoea was diagnosed after extensive evaluation as having collagenous colitis. Remarkable in this case was the definitely abnormal-looking mucosa in a period of clinical exacerbation whereas the subepithelial collagenous band completely disappeared in a clinically more quiet stage of the disease. The diagnostic importance of performing stepwise colonic biopsies even in normal-appearing colon in a patient with chronic undiagnosed diarrhoea is discussed.
Assuntos
Colite/patologia , Colágeno/análise , Mucosa Intestinal/patologia , Adulto , Biópsia , Colite/diagnóstico , Feminino , Humanos , Mucosa Intestinal/químicaAssuntos
Acantose Nigricans/complicações , Carcinoma de Células Escamosas/complicações , Neoplasias Pulmonares/complicações , Carcinoma de Células Escamosas/cirurgia , Humanos , Hipotireoidismo/complicações , Neoplasias Pulmonares/cirurgia , Masculino , Pessoa de Meia-Idade , Pneumonectomia , Fibrose Pulmonar/complicações , Fibrose Pulmonar/diagnóstico por imagem , Radiografia , Remissão EspontâneaAssuntos
Adenocarcinoma/cirurgia , Neoplasias da Mama/cirurgia , Leiomioma/cirurgia , Neoplasias Primárias Múltiplas , Neoplasias Uterinas/cirurgia , Feminino , Humanos , Leiomioma/complicações , Leiomiossarcoma , Neoplasias Pulmonares , Metrorragia/etiologia , Pessoa de Meia-Idade , Metástase Neoplásica , Osteoartropatia Hipertrófica Secundária/complicações , Neoplasias Uterinas/complicaçõesRESUMO
The development and application of nucleic acid sequence-based amplification (NASBA) assays for the detection of West Nile (WN) and St. Louis encephalitis (SLE) viruses are reported. Two unique detection formats were developed for the NASBA assays: a postamplification detection step with a virus-specific internal capture probe and electrochemiluminescence (NASBA-ECL assay) and a real-time assay with 6-carboxyfluorescein-labeled virus-specific molecular beacon probes (NASBA-beacon assay). The sensitivities and specificities of these NASBA assays were compared to those of a newly described standard reverse transcription (RT)-PCR and TaqMan assays for SLE virus and to a previously published TaqMan assay for WN virus. The NASBA assays demonstrated exceptional sensitivities and specificities compared to those of virus isolation, the TaqMan assays, and standard RT-PCR, with the NASBA-beacon assay yielding results in less than 1 h. These assays should be of utility in the diagnostic laboratory to complement existing diagnostic testing methodologies and as a tool in conducting flavivirus surveillance in the United States.
Assuntos
Vírus da Encefalite de St. Louis/isolamento & purificação , Encefalite de St. Louis/virologia , Replicação de Sequência Autossustentável/métodos , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/isolamento & purificação , Animais , Doenças das Aves/virologia , Aves/virologia , Chlorocebus aethiops , Culicidae/virologia , Vírus da Encefalite de St. Louis/genética , Sondas Moleculares , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Sensibilidade e Especificidade , Taq Polimerase/metabolismo , Fatores de Tempo , Células Vero , Febre do Nilo Ocidental/veterináriaRESUMO
Widespread deaths of American Crows (Corvus brachyrhynchos)were associated with the 1999 outbreak of West Nile (WN) virus in the New York City region. We compared six organs from 20 crow carcasses as targets for WN virus detection. Half the carcasses had at least one positive test result for WN virus infection. The brain was the most sensitive test organ; it was the only positive organ for three of the positive crows. The sensitivity of crow organs as targets for WN virus detection makes crow death useful for WN virus surveillance.
Assuntos
Doenças das Aves/virologia , Surtos de Doenças , Aves Canoras/virologia , Febre do Nilo Ocidental/veterinária , Vírus do Nilo Ocidental/isolamento & purificação , Doenças das Aves/patologia , New Jersey/epidemiologia , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Febre do Nilo Ocidental/patologia , Febre do Nilo Ocidental/virologia , Vírus do Nilo Ocidental/genéticaRESUMO
After the 1999 West Nile (WN) encephalitis outbreak in New York, 2,300 overwintering adult mosquitoes were tested for WN virus by cell culture and reverse transcriptase-polymerase chain reaction. WN viral RNA and live virus were found in pools of Culex mosquitoes. Persistence in overwintering Cx. pipiens may be important in the maintenance of WN virus in the northeastern United States.
Assuntos
Culex/virologia , Surtos de Doenças , Insetos Vetores/virologia , Vírus do Nilo Ocidental/isolamento & purificação , Aedes/citologia , Animais , Linhagem Celular , Chlorocebus aethiops , Cidade de Nova Iorque/epidemiologia , RNA Viral/análise , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estações do Ano , Células Vero , Vírus do Nilo Ocidental/genéticaRESUMO
The authors report on the development and application of a rapid TaqMan assay for the detection of West Nile (WN) virus in a variety of human clinical specimens and field-collected specimens. Oligonucleotide primers and FAM- and TAMRA-labeled WN virus-specific probes were designed by using the nucleotide sequence of the New York 1999 WN virus isolate. The TaqMan assay was compared to a traditional reverse transcriptase (RT)-PCR assay and to virus isolation in Vero cells with a large number ( approximately 500) of specimens obtained from humans (serum, cerebrospinal fluid, and brain tissue), field-collected mosquitoes, and avian tissue samples. The TaqMan assay was specific for WN virus and demonstrated a greater sensitivity than the traditional RT-PCR method and correctly identified WN virus in 100% of the culture-positive mosquito pools and 98% of the culture-positive avian tissue samples. The assay should be of utility in the diagnostic laboratory to complement existing human diagnostic testing and as a tool to conduct WN virus surveillance in the United States.