Low incidence of human coronavirus among hospitalized children in Novosibirsk city, Russia during pre-pandemic period (2013-2020).

We investigated the incidence of 15 respiratory viruses among 2991 children with acute respiratory infections in Novosibirsk city, Russia, prior to the COVID-19 pandemic (2013-2020). Viral infections were detected in 72.5% cases. The incidence of human coronavirus was 2% (Alphacoronaviruses, 63%; Betacoronaviruses, 37%).

[Changes in external respiration function in response to berotek and histamine in diabetic patients].

AIM: To study airways reactivity in patients with diabetes mellitus (DM). MATERIAL AND METHODS: Berotek and histamine tests were performed in 29 DM patients (type 1 DM - 20, type 2 DM - 9 patients) and 39 DM patients (12 and 27 patients, respectively). The age of the patients varied from 16 to 66 years. RESULTS: Positive berotek test was in 6 (20.7%) patients (delta FEV1 > 200). Of them 5 had type 1 DM. Positive histamine test was registered in 9 patients with type 2 DM (23.1%). This indicates bronchial hyperreactivity (BH). A qualitative analysis with Fisher's criterion detected correlation between BH and type of DM, BH and intake of sugar reducing tablets. Correlations between BH, body mass index, diabetes duration and late complications were not found. CONCLUSION: DM patients have predisposition to bronchial spasm. Positive bronchodilation test was mainly in type 1 DM, BH in response to histamine was obtained in type 2 DM. Different mechanisms of a bronchospasm in type 1 and 2 DM are demonstrated. Predisposition to bronchospasm may play an unfavourable role in the course of nonspecific pulmonary diseases in DM patients.

[Oxidative modification of blood proteins in patients with psychiatric disorders (depression, depersonalization)]. / Okislitel'naia modifikatsiia belkov plazmy krovi bol'nykh psikhicheskimi rasstroistvami (depressiia, depersonalizatsiia).

We determined the oxidative modification of proteins (spontaneous and metal-catalysing oxidation, MKO) and the level of corticosteroids in patients with the depersonalization and depression. For detecting oxidative modification of plasma proteins we measured the concentration of protein carbonyl groups formed with 2,4dinitrophenylhydrazine 2,4dinitrophenylhydrazone derivatives; the formation of dityrosine by fluorescence method; protein aggregation and fragmentation. Polyacrylamide gel electrophoresis with sodium dodecyl sulfate (SDS)-PAGE in the presence beta-mercaptoethanol was used to determine the aggregation or fragmentation of proteins by oxygen radicals (OH). Acid-soluble peptides were analised as products of the fragmentation oxidative modification proteins. The level of the corticosteroids was determined using HPLS. The increase of the concentration of protein carbonyl groups in blood plasma of patients with mental disorders. In patients with depersonalization we determined the increase of the bityrosyl cross-link, and different degrees of fragmentation compared with depressive patients. The cortisol level was decreased and corticosterone was increased in the blood plasma of patients with depersonalization. In depressive patients the cortisol level was increased and corticosterone was decreased is discussed. We discussed the role oxidative modification proteins in the disturbance of the corticosteroid and opioid receptors functions in the patients with mental disorders.

[Complex-formation between myeloperoxidase and fibronectin and its modulation by fibronectin ligands]. / Kompleksoobrazovanie mieloperoksidazy i fibronektina i ego moduliatsiia ligandami fibronektina.

Human blood plasma fibronectin immobilised on agarose in physiological interacts with soluble myeloperoxidase (Kd = 2.43 mM). Interaction of myeloperoxidase with fibronectin adsorbed on immobilised gelatin a, natural fibronectin ligand, resulted in formation more stable complex (Kd = 0.94 mM). The presence of thermoaggregated (but not native) IgG in the liquid phase increased a stability of the complex (0.06 mM). It is suggested that myeloperoxidase could represent a component of complex super molecular structure, real immune complex.

[Surgical treatment of pyo-necrotic forms of diabetic foot]. / Khirurgicheskoe lechenie gnoino-nekroticheskoi formy diabeticheskoi stopy.

The authors present the experience of combined surgical treatment of 94 patients with complicated "diabetic foot". In the majority of patients (92.8%) there was ischemic form of diabetic foot. Wide usage of staged necrectomies as treatment modalities, preferable use of economical amputations and exarticulations, continuous intensive intraarterial infusion (heparine, desaggregants, antibiotics, insulin suspension) contributed to preservation of the limbs in 82.6% of cases.

[Thermodynamic parameters of complex formation of blood plasma fibronectin and myeloperoxidase]. / Termodinmicheskie parametry kompleksoobrazovaniia fibronektina plazmy krovi i mieloperoksidazy.

Previous studies have demonstrated that fibronectin immobilized on BrCN-activated agarose forms a complex with soluble myeloperoxidase. The affinity of such interaction increases after preliminary absorption of fibronectin on a column with immobilized gelatin, one of its natural ligands. The thermodynamic characteristics of the myeloperoxidase interaction with fibronectin-gelatin-agarose have been established. The role of fibronectin-myeloperoxidase interaction in the inflammation focus is discussed in terms of the mode of the phagocyte loading with the enzyme and as a way of protecting intrinsic body tissues from injury by oxidants generated by extracellular myeloperoxidase.

Oxidative modification of proteins: oxidation of tryptophan and production of dityrosine in purified proteins using Fenton's system.

Specific features of metal-catalyzed oxidation (MCO) of purified proteins (human serum albumin and human erythrocyte superoxide dismutase) were analyzed by the oxidation level of tryptophan and tyrosine. The production of dityrosine cross-links and the oxidation of tryptophan residues were recorded by fluorescence. The degree of oxidative modification of the amino acid residues of the proteins depended on the concentration of the Fenton's medium components and on the incubation time. These changes were different in different proteins. By electrophoresis and gel-permeation chromatography, changes in the superoxide dismutase structure are shown to be caused by oxidative modification of the enzyme and to be accompanied by a decrease in its activity. Findings with OH* scavengers (mannitol and ethanol) suggest that oxidative modification of the proteins in Fenton's medium should be associated not only with hydroxyl radical but also with ferryl and perferryl ions and with the radical CO(-.)(3).

[Oxidative modification of blood plasma proteins in elderly people with vascular dementia]. / Okislitel'naia modifikatsiia belkov plazmy krovi u pozhilykh liudei s sosudistoi dementsiei.

We examined the spontaneous and metal-ion-catalyzed oxidative modification plasma blood proteins in the group of healthy adults and elderly ones and patients with vascular dementia (mild and severe). We determined the spectrophotometric measurement of 2,4-dinitrophenylhydrazone derivatives formed by reactions with protein carbonyls. The level of metal-ion-catalyzed oxidation proteins in the aged patients both with and without dementia was high in comparison to the healthy adults. The patients with severe dementia showed lower amount of 2,4-dinitrophenylhydrazone deriviates. Low levels of metal-ion-catalysed protein oxidation strongly correlated with the degree of psychoorganic disturbances. The elderly persons with both and without dementia showed a high level of plasma nonenzymatic H2O2 scavenging in comparison with the healthy adult ones. We discovered an imbalance between enzymatic and nonenzymatic components of the antioxidant system. The latter indicates that the oxidative modification of brain tissue proteins probably plays an important role in aging and mental disorders.