Superionic iron alloys and their seismic velocities in Earth's inner core.

Earth's inner core (IC) is less dense than pure iron, indicating the existence of light elements within it1. Silicon, sulfur, carbon, oxygen and hydrogen have been suggested to be the candidates2,3, and the properties of iron-light-element alloys have been studied to constrain the IC composition4-19. Light elements have a substantial influence on the seismic velocities4-13, the melting temperatures14-17 and the thermal conductivities18,19 of iron alloys. However, the state of the light elements in the IC is rarely considered. Here, using ab initio molecular dynamics simulations, we find that hydrogen, oxygen and carbon in hexagonal close-packed iron transform to a superionic state under the IC conditions, showing high diffusion coefficients like a liquid. This suggests that the IC can be in a superionic state rather than a normal solid state. The liquid-like light elements lead to a substantial reduction in the seismic velocities, which approach the seismological observations of the IC20,21. The substantial decrease in shear-wave velocity provides an explanation for the soft IC21. In addition, the light-element convection has a potential influence on the IC seismological structure and magnetic field.

Effect of Intermittent Oro-Esophageal Tube Feeding in Bulbar Palsy After Ischemic Stroke: A Randomized Controlled Study.

BACKGROUND: Nasogastric tube feeding (NG) has been widely used in patients with bulbar palsy after ischemic stroke but is associated with a significant risk of complications including malnutrition and pneumonia. Intermittent oro-esophageal tube feeding (IOE) can help alleviate these concerns. This study explored the clinical effect of IOE versus NG on nutritional status, swallowing function, stroke-associated pneumonia, and depression in patients with bulbar palsy after ischemic stroke. METHODS: This randomized controlled study included 148 patients with bulbar palsy after ischemic stroke who underwent routine treatment and swallowing rehabilitation training in the Department of Rehabilitation Medicine between July 2017 and July 2019 in China. The participants were randomly divided into the IOE group (n=74) and NG group (n=74) with IOE and NG as nutritional supports, respectively. The primary outcome was nutritional status including (1) body mass index (kg/m2), (2) serum ALB (albumin, g/L), and (3) PA (prealbumin, mg/L). The secondary outcomes were (1) swallowing function including (i) Functional Oral Intake Scale (FOIS) and (ii) Penetration-Aspiration Scale, (2) pneumonia, (3) depression, and (4) adverse events. Statistical analyses for continuous outcomes were performed using t test, Mann-Whitney U test and Wilcoxon signed-rank test and categorical variables using χ2 test. SPSS 21.0 was used for all analysis. RESULTS: There were no significant baseline differences between the 2 groups. After the treatment, the IOE group demonstrated significantly better results compared with the NG group in ALB ([32.71±0.94] versus [32.28±0.81] g/L; P=0.003), PA ([278.15±13.81] versus [270.31±15.08] mg/L; P=0.001], body mass index ([19.77±1.03] versus [19.41±0.98] kg/m2; P=0.002], FOIS (P<0.001), Penetration-Aspiration Scale (P<0.001), stroke-associated pneumonia ([1, 4.05%] versus [26, 35.14%]; P<0.001), depression ([1, 1.35%] versus [44, 59.46%]; P<0.001) and overall less adverse events (reflux, fever, discomfort in the throat; P<0.001). CONCLUSIONS: In patients with dysphagia with bulbar palsy after ischemic stroke who received routine treatment and swallowing rehabilitation training, IOE is safer and more conducive to the improvement of nutritional status, swallowing function, stroke-associated pneumonia, and depression than NG. REGISTRATION: URL: https://www.chictr.org.cn; Unique identifier: ChiCTR-INC-17011741.

A consensus genome of sika deer (Cervus nippon) and transcriptome analysis provided novel insights on the regulation mechanism of transcript factor in antler development.

BACKGROUND: Sika deer (Cervus nippon) holds significance among cervids, with three genomes recently published. However, these genomes still contain hundreds of gaps and display significant discrepancies in continuity and accuracy. This poses challenges to functional genomics research and the selection of an appropriate reference genome. Thus, obtaining a high-quality reference genome is imperative to delve into functional genomics effectively. FINDINGS: Here we report a high-quality consensus genome of male sika deer. All 34 chromosomes are assembled into single-contig pseudomolecules without any gaps, which is the most complete assembly. The genome size is 2.7G with 23,284 protein-coding genes. Comparative genomics analysis found that the genomes of sika deer and red deer are highly conserved, an approximately 2.4G collinear regions with up to 99% sequence similarity. Meanwhile, we observed the fusion of red deer's Chr23 and Chr4 during evolution, forming sika deer's Chr1. Additionally, we identified 607 transcription factors (TFs) that are involved in the regulation of antler development, including RUNX2, SOX6, SOX8, SOX9, PAX8, SIX2, SIX4, SIX6, SPI1, NFAC1, KLHL8, ZN710, JDP2, and TWST2, based on this consensus reference genome. CONCLUSIONS: Our results indicated that we acquired a high-quality consensus reference genome. That provided valuable resources for understanding functional genomics. In addition, discovered the genetic basis of sika-red hybrid fertility and identified 607 significant TFs that impact antler development.

Detoxifying bacterial genes for deoxynivalenol epimerization confer durable resistance to Fusarium head blight in wheat.

Fusarium head blight (FHB) and the presence of mycotoxin deoxynivalenol (DON) pose serious threats to wheat production and food safety worldwide. DON, as a virulence factor, is crucial for the spread of FHB pathogens on plants. However, germplasm resources that are naturally resistant to DON and DON-producing FHB pathogens are inadequate in plants. Here, detoxifying bacteria genes responsible for DON epimerization were used to enhance the resistance of wheat to mycotoxin DON and FHB pathogens. We characterized the complete pathway and molecular basis leading to the thorough detoxification of DON via epimerization through two sequential reactions in the detoxifying bacterium Devosia sp. D6-9. Epimerization efficiently eliminates the phytotoxicity of DON and neutralizes the effects of DON as a virulence factor. Notably, co-expressing of the genes encoding quinoprotein dehydrogenase (QDDH) for DON oxidation in the first reaction step, and aldo-keto reductase AKR13B2 for 3-keto-DON reduction in the second reaction step significantly reduced the accumulation of DON as virulence factor in wheat after the infection of pathogenic Fusarium, and accordingly conferred increased disease resistance to FHB by restricting the spread of pathogenic Fusarium in the transgenic plants. Stable and improved resistance was observed in greenhouse and field conditions over multiple generations. This successful approach presents a promising avenue for enhancing FHB resistance in crops and reducing mycotoxin contents in grains through detoxification of the virulence factor DON by exogenous resistance genes from microbes.

Overexpression of TNFSF11 reduces GPX4 levels and increases sensitivity to ferroptosis inducers in lung adenocarcinoma.

BACKGROUND: Lung adenocarcinoma (LUAD), the most common and lethal subtype of lung cancer, continues to be a major health concern worldwide. Despite advances in targeted and immune therapies, only a minority of patients derive substantial benefits. As a result, the urgent need for novel therapeutic strategies to improve lung cancer treatment outcomes remains undiminished. METHODS: In our study, we employed the TIMER database to scrutinize TNFSF11 expression across various cancer types. We further examined the differential expression of TNFSF11 in normal and tumor tissues utilizing the TCGA-LUAD dataset and tissue microarray, and probed the associations between TNFSF11 expression and clinicopathological parameters within the TCGA-LUAD dataset. We used the GSE31210 dataset for external validation. To identify genes strongly linked to TNFSF11, we engaged LinkedOmics and conducted a KEGG pathway enrichment analysis using the WEB-based Gene SeT AnaLysis Toolkit. Moreover, we investigated the function of TNFSF11 through gene knockdown or overexpression approaches and explore its function in tumor cells. The therapeutic impact of ferroptosis inducers in tumors overexpressing TNFSF11 were also investigated through in vivo and in vitro experiments. Through these extensive analyses, we shed light on the potential role of TNFSF11 in lung adenocarcinoma, underscoring potential therapeutic targets for this malignancy. RESULTS: This research uncovers the overexpression of TNFSF11 in LUAD patients and its inverse correlation with peroxisome-related enzymes. By utilizing gene knockdown or overexpression assays, we found that TNFSF11 was negatively associated with GPX4. Furthermore, cells with TNFSF11 overexpression were relatively more sensitive to the ferroptosis inducers. CONCLUSIONS: Our research has provided valuable insights into the role of TNFSF11, revealing its negative regulation of GPX4, which could be influential in crafting therapeutic strategies. These findings set the stage for further exploration into the mechanisms underpinning the relationship between TNFSF11 and GPX4, potentially opening up new avenues for precision medicine in the treatment of LUAD.

Efficient tunable cascaded Raman source with all-silica fibers based on 2-µm DSR pulse pumping.

We present an efficient tunable all-silica-fiber 2nd-order cascaded Raman pulse laser utilizing 2-µm dissipative-soliton-resonance (DSR) rectangular pulses for pumping and highly GeO2-doped silica fiber as Raman gain medium. When pumped at 1966.5 nm, the maximum 1st-order Raman optical conversion efficiency is up to 64.4% at 2153 nm, with 92.4% spectral purity and 0.39-W average power. The maximum 2nd-order Raman optical conversion efficiency is 19.3% at 2370 nm, with 39.2% spectral purity and 0.25-W average power. To our knowledge, these conversion efficiencies and spectral purities represent the highest levels achieved in a mid-infrared all-silica-fiber cascaded pulsed Raman laser. Additionally, by adjusting the central wavelength of the DSR seed pulse, the 2nd-order Raman light can be tuned within a range of 41 nm (2354∼2395 nm). Our system provides a simple and easy-to-implement solution for realizing efficient tunable cascaded pulsed Raman lasers in the 2.4-µm band.

Controllable non-uniformly distributed spiking cluster generation in broadband optoelectronic oscillator.

An approach to achieve controllable non-uniformly distributed spiking cluster generation is proposed and demonstrated based on an externally-triggered broadband optoelectronic oscillator (OEO). The theory of controlling the distribution of the spiking pulses in a spiking cluster is established. Based on the theory, the dynamic and the distribution characteristics are analyzed and revealed in the stable spiking oscillation state under different externally-injected trigger signal voltages. The peak-voltage envelop of the cluster and the interval of the spiking pulses are demonstrated to have an approximate negative linearity relationship with the externally-injected trigger signal voltage in both the numerical simulation and the experiment, where a square waveform, a trapezoidal waveform, a parabola waveform, and a half-sinusoidal waveform are used as the externally-injected trigger signals. The results indicate that the spiking pulse distribution in the generated spiking cluster can be well controlled through tuning the externally-injected trigger signal voltage. The proposed scheme can be utilized in spiking encoding and reservoir computing.

Phase-locked dual-frequency microwave signal generation in an optoelectronic oscillator based on frequency mixing mutual injection.

An approach to generating stable phase-locked dual-frequency microwave signals is proposed and demonstrated based on a dual-passband optoelectronic oscillator (OEO). Mode gain competition is broken by employing frequency mixing mutual injection effect to realize phase locking between the two oscillation signals, which is achieved by applying a single-tone signal to a microwave mixer in the OEO cavity. In addition, a dual-loop configuration with balanced detection is utilized to ensure a high side mode suppression ratio (SMSR) and ultra-low phase noise, which also enhances the stability of the generated signal. In the experiment, a phase-locked dual-frequency microwave signal at 9.9982 GHz and 10.1155 GHz is generated by using the proposed OEO scheme. The SMSR and the phase noise are 75 dB and -141 dBc/Hz@10 kHz, respectively. Additionally, the Allan deviation of the generated signal is in the order of 10-11@1 s. These parameters are superior to those based on the same OEO but with a single-loop configuration, which are also compared in detail.

Successful immunotherapy with PD-1 Iinhibitor for advanced pancreatic cancer: report of two cases and review of literature.

Pancreatic cancer is a highly malignant tumor, and most patients are diagnosed at an advanced stage. Unfortunately, due to the immunosuppressive tumor microenvironment of pancreatic cancer, the benefits of immunotherapy for patients with advanced pancreatic cancer are still unclear. Here, we present two cases of advanced pancreatic cancer being controlled by immunotherapy, with pathological diagnoses of ductal adenocarcinoma and acinar cell carcinoma, respectively. Next-generation sequencing (NGS) of both patients is high tumor mutation burden (tumor mutation burden-High) and microsatellite stable. The patient with pancreatic ductal adenocarcinoma was diagnosed as a locally advanced disease (stage III). She received irreversible electroporation, used the programmed death receptor-1 (PD-1) inhibitor (pembrolizumab) combined with chemotherapy (S-1), and then used only the PD-1 inhibitor as a maintenance treatment. As a result, the patient's lesion was significantly reduced, with a partial response time of up to 31 months. The patient with acinar cell carcinoma was diagnosed as a metastatic disease (stage IV), next-generation sequencing revealed mutations in SMAD4 and KMT2D, and two chemotherapy regimens were used unsuccessfully. Then, the combination of chemotherapy with PD-1 (tislelizumab) and vascular endothelial growth factor/vascular endothelial growth factor receptor (anlotinib) inhibitors were used, and the lesions of the patient were significantly reduced, and the progression-free survival after immunotherapy was 19 months. In advanced pancreatic cancer, a prognosis of this magnitude is rare. Our cases reveal the potential of immunotherapy as a cornerstone treatment in the management of advanced pancreatic cancer.

Molecular mechanisms of HCG18 in the sorafenib resistance of hepatocellular carcinoma.

Sorafenib has been approved for advance hepatocellular carcinoma (HCC), however, drug resistance often occurred. Therefore, it is of great significance to clarify the underlying mechanisms of sorafenib resistance and to find out the effective strategies to overcome sorafenib resistance. The expression of HCG18 was detected by qPCR, MTT, colony formation, flow cytometry and TUNEL assay were used to explore the function of HCG18 on sorafenib resistance in HCC. RNA pull-down, RNA immunoprecipitation, immunofluorescence labeling, luciferase reporter assay, western blot and qPCR were used to investigate the mechanism of HCG18 regulating sorafenib resistance in HCC. Our results showed that HCG18 was significantly increased in HCC, which resulted in shorter 5-year survival for patients with HCC. Sorafenib can induce the expression of HCG18, suggesting HCG18 might be involved in sorafenib resistance in HCC. Further analysis showed that knockdown of HCG18 can reduce viability and increase apoptosis of HCC cells. Mechanistically, HCG18 can bind to USP15, further regulated the protein stability of p65, TAB2 and TAB3, and nuclear location of p65, which finally modulated the NF-κB signaling. Our findings showed that HCG18 played an important role in sorafenib resistance in HCC. And knockdown of HCG18 can promote the sensitivity of HCC cells to sorafenib, inferring that targeting HCG18 might be an effective strategy to overcome sorafenib resistance in HCC.

A Enhanced Fluorescent Probe for Simultaneous Detection and Discrimination of Hydrogen Bisulfite Anions and Glutathione.

Bisulfite (HSO3-) and biological thiols molecules, such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy), play important roles in organisms. Developing a fluorescent probe that can simultaneously detect and distinguish HSO3- and biological thiols is of great significance. In this study, ethyl(2E,4Z)-5-chloro-2-cyano-5-(7-(diethylamino)-2-oxo-2 H-chromen-3-yl)penta-2,4-dienoate (CCO) as a novel enhanced fluorescence probe was synthesized by integrating coumarin derivatives and ethyl cyanoacetate, which can simultaneous detection and discrimination of hydrogen bisulfite anions and glutathione. The sensing mechanism was elucidated through spectral analysis and some control experiments. In weakly alkaline environments, the probe not only has good selectivity for HSO3- and GSH, but also has a lower detection limits of 0.0179 µM and 0.2034 µM. The probe exhibited fuorescent turn-on for distinguishing with 296 and 28 fold the fluorescent intensity increase at 486 and 505 nm, respectively, through diferent excitation wavelengths. This provides a new method for simultaneous detection and discrimination of HSO3- and biological thiol cell levels and further applications.

Analysis of risk factors and development of predictive model for malnutrition in patients with traumatic brain injury.

Malnutrition is a highly prevalent complication in patients with traumatic brain injury (TBI), and it is closely related to the prognosis of patients. Accurate identification of patients at high risk of malnutrition is essential. Therefore, we analyzed the risk factors of malnutrition in patients with TBI and developed a model to predict the risk of malnutrition. A retrospective collection of 345 patients with TBI, and they were divided into malnutrition and comparison groups according to the occurrence of malnutrition. Univariate correlation and multifactor logistic regression analyses were performed to determine patients' malnutrition risk factors. We used univariate and logistic regression (forward stepwise method) analyses to identify significant predictors associated with malnutrition in patients with TBI and developed a predictive model for malnutrition prediction. The model's discrimination, calibration, and clinical utility were evaluated using the receiver operating characteristic (ROC) curve, calibration plots, and decision curve analysis (DCA). A total of 216 patients (62.6%) developed malnutrition. Multifactorial logistic regression analysis showed that pulmonary infection, urinary tract infection, dysphagia, application of NGT, GCS score ≤ 8, and low ADL score were independent risk factors for malnutrition in patients with TBI (P < 0.05). The area under the curve of the model was 0.947. Calibration plots showed good discrimination of model calibration. DCA showed that the column line plot models were all clinically meaningful when nutritional interventions were performed over a considerable range of threshold probabilities (0-0.98). Malnutrition is widespread in patients with TBI, and the nomogram is a good predictor of whether patients develop malnutrition.

A straightforward access to trifluoromethylated natural products through late-stage functionalization.

Covering: 2011 to 2021Trifluoromethyl (CF3)-modified natural products have attracted increasing interest due to their magical effect in binding affinity and/or drug metabolism and pharmacokinetic properties. However, the chemo and regioselective construction of natural products (NPs) bearing a CF3 group still remains a long-standing challenge due to the complex chemical scaffolds and diverse reactive sites of NPs. In recent years, the development of late-stage functionalization strategies, including metal catalysis, organocatalysis, light-driven reactions, and electrochemical synthesis, has paved the way for direct trifluoromethylation process. In this review, we summarize the applications of these strategies in the late-stage trifluoromethylation of natural products in the past ten years with particular emphasis on the reaction model of each method. We also discuss the challenges, limitations, and future prospects of this approach.

High-resolution radar ranging based on the ultra-wideband chaotic optoelectronic oscillator.

A high-resolution radar ranging scheme is proposed and demonstrated based on the ultra-wideband chaotic optoelectronic oscillator (OEO). Through biasing the electro-optic intensity modulator near its minimum transmission point, high-dimensional chaotic signals with flat spectra and low time-delayed signatures can be generated in the OEO, which are favorable for increasing the ranging resolution and the confidentiality. In the experiment, the optimized broadband OEO generates a high-dimensional chaotic signal with a flat spectrum in the frequency range of 2 GHz to 16 GHz and a high permutation entropy of 0.9754. This chaotic signal is used to achieve multiple target ranging, where a ranging resolution of 1.4 cm is realized.

Characterization of high-speed electro-optic phase modulators based on heterodyne carrier mapping at a fixed low-frequency.

A self-referenced method based on heterodyne carrier mapping is proposed to characterize the modulation efficiency of high-speed electro-optic phase modulators (EOPMs). The heterodyne carrier mapping replicates the optical carrier after phase modulation to an electrical replica, which enables observing the power variation of the optical carrier at a fixed low-frequency in the electrical domain. The modulation depths and half-wave voltages within the frequency range of up to 40 GHz are determined by measuring the amplitude ratio of the mapped low-frequency component at 80 MHz in the cases of on and off single-tone modulation of the EOPM. The measured results are compared to those obtained with the traditional optical spectrum analysis method and the electrical spectrum sweep method to check the consistency and accuracy. Surpassing the heterodyne spectrum mapping (HSM) scheme, our method only requires a single-tone driving of the EOPM under test and completely avoids the roll-off responsivity of the photodetector through the fixed low-frequency detection.

Neuromorphic regenerative memory optoelectronic oscillator.

Neuromorphic spiking information processing based on neuron-like excitable effect has achieved rapid development in recent years due to its advantages such as ultra-high operation speed, programming-free implementation and low power consumption. However, the current physical platforms lack building blocks like compilers, logic gates, and more importantly, data memory. These factors become the shackles to construct a full-physical layer neural network. In this paper, a neuromorphic regenerative memory scheme is proposed based on a time-delayed broadband nonlinear optoelectronic oscillator (OEO), which enables reshaping and regenerating on-off keying encoding sequences. Through biasing the dual-drive Mach-Zehnder electro-optic modulator in the OEO cavity near its minimum transmission point, the OEO can work in excitable regime, where localized states are maintained for robust nonlinear spiking response. Both simulation and experiment are carried out to demonstrate the proposed scheme, where the simulation results and the experimental results fit in with each other. The proposed OEO-based neuromorphic regenerative memory scheme exhibits long-term response ability for short-term excitation, which shows an enormous application potential for high-speed neuromorphic information buffering, optoelectronic interconnection and computing.

Instantaneous bandwidth expansion of photonic sampling analog-to-digital conversion for linear frequency modulation waveforms based on up-sampling and fractional Fourier transform signal processing.

An approach to expanding the instantaneous bandwidth of a photonic sampling analog-to-digital converter (ADC) for receiving linear frequency modulation waveforms (LFMWs) is proposed and experimentally demonstrated based on up-sampling and filtering in the fractional Fourier domain. Through twice zero interpolation, the equivalent sampling rate is quadrupled, which also quadruples the nominal instantaneous bandwidth of the photonic sampling ADC. In addition, with the assistance of bandpass filtering in the fraction Fourier domain, the image signals and the harmonic distortions generated in the interpolation process are filtered out. As a result, the effective instantaneous bandwidth of the photonic sampling ADC is doubled. In the experiment, the instantaneous bandwidth of a photonic sampling ADC with a sampling rate of 5 GSa/s for receiving LFMWs is increased from 2.5 GHz to 5 GHz by using the proposed method. Input LFMWs within the frequency range of 24-27 GHz and 30-33 GHz, i.e., with an instantaneous bandwidth of 3 GHz, are digitized without frequency-domain aliasing. Besides, the ability of the proposed method to enhance the ranging accuracy in a broadband radar system is demonstrated. This method reduces the hardware complexity of the photonic sampling ADC for receiving broadband LFMWs in radar systems.

Time-delay signature suppression of the chaotic signal in a semiconductor laser based on optoelectronic hybrid feedback.

An approach to generating chaotic signals with low time-delay signatures (TDSs) from a semiconductor laser (SCL) is proposed and demonstrated based on optoelectronic hybrid feedback. Through using a chirped fiber Bragg grating (CFBG) to provide distributed feedback, a chaotic signal with a low TDS is generated from the SCL. With the assistance of the nonlinear optoelectronic feedback provided by a microwave photonic link, the relaxation oscillation effect in the SCL is effectively suppressed, and the periodicity of the oscillation is greatly weakened. Hence, the TDS of the generated chaotic signal from the SCL is further suppressed, and the effective bandwidth is enlarged. Both simulation and experiment are carried out to verify the feasibility of the proposed scheme to suppress the TDS. In the experiment, a chaotic signal with a large effective bandwidth of 12.93 GHz, an extremely high permutation entropy (PE) of 0.9983, and a low TDS of 0.04, is generated by using a CFBG with a dispersion coefficient of 22.33 ps/nm. This TDS value is at the same level as that obtained by using the SCL-based scheme relying solely on distributed feedback in a CFBG with a dispersion coefficient of 2000 ps/nm.

Pulse generation with programmable positions based on a phase-modulated optical frequency-shifting loop.

An approach to generating pulses with programmable positions is proposed and demonstrated based on a phase-modulated optical frequency-shifting loop (OFSL). By setting the OFSL to operate in the integer Talbot state, pulses are generated in the phase-locked positions, since the additional phase introduced by the electro-optic phase modulator (PM) in the OFSL is equal to an integer multiple of 2π in each round trip. Therefore, the pulse positions can be controlled and encoded by designing the driving waveform of the PM in a round-trip time. In the experiment, linear, round-trip, quadratic, and sinusoidal variations of pulse intervals are achieved by applying the corresponding driving waveforms to the PM. Pulse trains with coded pulse positions are also realized. In addition, the OFSL driven by waveforms with repetition rates equal to double and triple the free spectral range of the loop is also demonstrated. The proposed scheme paves a way to generate optical pulse trains with user-defined pulse positions, which can be used for such applications as compressed sensing and lidar.

Elevated FAM134B expression induces radiation-sensitive in hepatocellular carcinoma.

BACKGROUND: Previous studies have shown that Family with sequence similarity 134 member B (FAM134B) was involved in the occurrence and development of malignancy, however, the function and molecular mechanism of FAM134B in Hepatocellular Carcinoma (HCC) radiotherapy resistance remain unclear. Therefore, it may clinical effective to clarify the molecular mechanism and identify novel biomarker to overcome radiotherapy resistance in HCC. METHODS: The protein and mRNA expression of FAM134B were determined using Real-time PCR and Western blot, respectively. IHC assay was performed to investigate the association between FAM134B expression and the clinicopathological characteristics of 132 HCC patients. Functional assays, such as in situ model, colon formation, FACS, and Tunel assay were used to determine the oncogenic role of FAM134B in human HCC progression. Furthermore, western blotting and luciferase assay were used to determine the mechanism of FAM134B promotes radiation-sensitive in HCC cells. RESULTS: We noted that FAM134B was downregulated in HCC, which was correlated with the radiation resistance in patients with HCC. Overexpression of FAM134B contribute to radiation sensitive in HCC; however, inhibition of FAM134B confers HCC cell lines to radiation resistance both in vitro and in vivo. Moreover, we found that FAM134B interacts with FMS related receptor tyrosine kinase 3 (FLT3) and downregulation of FAM134B activated JAK/Stat3 signaling pathway. Importantly, pharmacological inhibition of JAK/Stat3 signaling pathway significantly counteracted downregulation of FAM134B-induced radiation resistance and enhanced radiation therapeutic efficacy in HCC. CONCLUSIONS: Our findings suggest that FAM134B may be a potential therapeutic biomarker for the treatment of HCC patients with radiotherapy tolerance.