Emergence of a clinical Salmonella enterica serovar 1,4,[5], 12: i:-isolate, ST3606, in China with susceptibility decrease to ceftazidime-avibactam carrying a novel blaCTX-M-261 variant and a blaNDM-5.

PURPOSE: The detection rate of Salmonella enterica serovar 1,4,[5], 12: i: - (S. 1,4,[5], 12: i: -) has increased as the most common serotype globally. A S. 1,4,[5], 12: i: - strain named ST3606 (sequence type 34), isolated from a fecal specimen of a child with acute diarrhea hospitalized in a tertiary hospital in China, was firstly reported to be resistant to carbapenem and ceftazidime-avibactam. The aim of this study was to characterize the whole-genome sequence of S. 1,4,[5], 12: i: - isolate, ST3606, and explore its antibiotic resistance genes and their genetic environments. METHODS: The genomic DNA of S. 1,4,[5], 12: i: - ST3606 was extracted and performed with single-molecule real-time sequencing. Resistance genes, plasmid replicon type, mobile elements, and multilocus sequence types (STs) of ST3606 were identified by ResFinder 3.2, PlasmidFinder, OriTfinder database, ISfinder database, and MLST 2.0, respectively. The conjugation experiment was utilized to evaluate the conjugation frequency of pST3606-2. Protein expression and enzyme kinetics experiments of CTX-M were performed to analyze hydrolytic activity of a novel CTX-M-261 enzyme toward several antibiotics. RESULTS: Single-molecule real-time sequencing revealed the coexistence of a 109-kb IncI1-Iα plasmid pST3606-1 and a 70.5-kb IncFII plasmid pST3606-2. The isolate carried resistance genes, including blaNDM-5, sul1, qacE, aadA2, and dfrA12 in pST3606-1, blaTEM-1B, aac(3)-lld, and blaCTX-M-261, a novel blaCTX-M-1 family member, in pST3606-2, and aac(6')-Iaa in chromosome. The blaCTX-M-261 was derived from blaCTX-M-55 by a single-nucleotide mutation 751G>A leading to amino acid substitution of Val for Met at position 251 (Val251Met), which conferred CTX-M increasing resistance to ceftazidime verified by antibiotics susceptibility testing of transconjugants carrying pST3606-2 and steady-state kinetic parameters of CTX-M-261. pST3606-1 is an IncI1-α incompatibility type that shares homology with plasmids of pC-F-164_A-OXA140, pE-T654-NDM-5, p_dm760b_NDM-5, and p_dmcr749c_NDM-5. The conjugation experiment demonstrated that pST3606-2 was successfully transferred to the Escherichia coli recipient C600 with four modules of OriTfinder. CONCLUSION: Plasmid-mediated horizontal transfer plays an important role in blaNDM-5 and blaCTX-M-261 dissemination, which increases the threat to public health due to the resistance to most ß-lactam antibiotics. This is the first report of blaCTX-M-261 and blaNDM-5 in S. 1,4,[5], 12: i: -. The work provides insights into the enzymatic function and demonstrates the ongoing evolution of CTX-M enzymes and confirms urgency to control resistance of S. 1,4,[5], 12: i: -.

The alleviative effect of C-phycocyanin peptides against TNBS-induced inflammatory bowel disease in zebrafish via the MAPK/Nrf2 signaling pathways.

INTRODUCTION: Ulcerative colitis (UC) is an incurable and highly complex chronic inflammatory bowel disease (IBD) affecting millions of people worldwide. C-phycocyanin (C-PC) has been reported to possess outstanding anti-inflammatory activities and can effectively inhibit various inflammation-related diseases. Whether C-PC-derived bioactive peptides can inhibit intestinal inflammation is worth research and consideration. METHODS: The inhibition activities of three anti-neuroinflammatory peptides were evaluated using 2-4-6-trinitrobenzen sulfonic acid (TNBS)-induced zebrafish colitis model. Subsequently, the abilities of peptides to promote gastrointestinal motility were also examined. The changes in the intestinal pathological symptoms and ultrastructure of intestinal, reactive oxygen species (ROS) levels, and antioxidant enzymes were then determined after co-treatment with peptides and TNBS. Transcriptome analysis was used to investigate the underlying ameliorating TNBS-induced colitis effects molecular mechanisms of better activity peptide. Furthermore, quantitative reverse-transcription polymerase chain reaction and molecular docking techniques verified the mRNA sequencing results. RESULTS: Three peptides, MHLWAAK, MAQAAEYYR and MDYYFEER, which significantly inhibit macrophage migration, were synthesized. The results showed that these peptides could effectively alleviate the inflammatory responses in the TNBS-induced zebrafish model of colitis. In addition, co-treatment with TNBS and C-PC peptides could decrease ROS production and increase antioxidant enzyme activities in zebrafish larvae. Moreover, MHLWAAK had the most significantly therapeutic effects on colitis in zebrafish. The transcriptome analysis suggests that the effect of MHLWAAK on TNBS-induced colitis may be associated with the modulation of nuclear factor-erythroid 2-related factor 2 (Nrf2) and mitogen-activated protein kinase (MAPK) signaling pathway associated genes. In addition, molecular docking was conducted to study the prospective interaction between peptides and the key proteins that streamline the Nrf2 and MAPK signaling pathways. IL-6, JNK3, TNF-α, KEAP1-NRF2 complex and MAPK may be the core targets of MHLWAAK in treating colitis. CONCLUSION: The results suggested that the three C-PC-derived peptides could ameliorate TNBS-induced colitis in zebrafish, and these peptides might be a promising therapeutic candidate for UC treatment.

Marine-Fungus-Derived Natural Compound 4-Hydroxyphenylacetic Acid Induces Autophagy to Exert Antithrombotic Effects in Zebrafish.

Marine natural products are important sources of novel drugs. In this study, we isolated 4-hydroxyphenylacetic acid (HPA) from the marine-derived fungus Emericellopsis maritima Y39-2. The antithrombotic activity and mechanism of HPA were reported for the first time. Using a zebrafish model, we found that HPA had a strong antithrombotic activity because it can significantly increase cardiac erythrocytes, blood flow velocity, and heart rate, reduce caudal thrombus, and reverse the inflammatory response caused by Arachidonic Acid (AA). Further transcriptome analysis and qRT-PCR validation demonstrated that HPA may regulate autophagy by inhibiting the PI3K/AKT/mTOR signaling pathway to exert antithrombotic effects.

Duplex-Hierarchy Representation Learning for Remote Sensing Image Classification.

Remote sensing image classification (RSIC) is designed to assign specific semantic labels to aerial images, which is significant and fundamental in many applications. In recent years, substantial work has been conducted on RSIC with the help of deep learning models. Even though these models have greatly enhanced the performance of RSIC, the issues of diversity in the same class and similarity between different classes in remote sensing images remain huge challenges for RSIC. To solve these problems, a duplex-hierarchy representation learning (DHRL) method is proposed. The proposed DHRL method aims to explore duplex-hierarchy spaces, including a common space and a label space, to learn discriminative representations for RSIC. The proposed DHRL method consists of three main steps: First, paired images are fed to a pretrained ResNet network for extracting the corresponding features. Second, the extracted features are further explored and mapped into a common space for reducing the intra-class scatter and enlarging the inter-class separation. Third, the obtained representations are used to predict the categories of the input images, and the discrimination loss in the label space is minimized to further promote the learning of discriminative representations. Meanwhile, a confusion score is computed and added to the classification loss for guiding the discriminative representation learning via backpropagation. The comprehensive experimental results show that the proposed method is superior to the existing state-of-the-art methods on two challenging remote sensing image scene datasets, demonstrating that the proposed method is significantly effective.

Anti-Tumor Activity and Mechanism of Silibinin Based on Network Pharmacology and Experimental Verification.

Silibinin is a flavonoid compound extracted from the seeds of Silybum marianum (L.) Gaertn. It has the functions of liver protection, blood-lipid reduction and anti-tumor effects. However, the potential molecular mechanism of silibinin against tumors is still unknown. This study aimed to assess the anti-tumor effects of silibinin in adenoid cystic carcinoma (ACC2) cells and Balb/c nude mice, and explore its potential mechanism based on network pharmacology prediction and experimental verification. A total of 347 targets interacting with silibinin were collected, and 75 targets related to the tumor growth process for silibinin were filtrated. Based on the PPI analysis, CASP3, SRC, ESR1, JAK2, PRKACA, HSPA8 and CAT showed stronger interactions with other factors and may be the key targets of silibinin for treating tumors. The predicted target proteins according to network pharmacology were verified using Western blot analysis in ACC2 cells and Balb/c nude mice. In the pharmacological experiment, silibinin was revealed to significantly inhibit viability, proliferation, migration and induce the apoptosis of ACC2 cells in vitro, as well as inhibit the growth and development of tumor tissue in vivo. Western blot analysis showed that silibinin affected the expression of proteins associated with cell proliferation, migration and apoptosis, such as MMP3, JNK, PPARα and JAK. The possible molecular mechanism involved in cancer pathways, PI3K-Akt signaling pathway and viral carcinogenesis pathway via the inhibition of CASP3, MMP3, SRC, MAPK10 and CDK6 and the activation of PPARα and JAK. Overall, our results provided insight into the pharmacological mechanisms of silibinin in the treatment of tumors. These results offer a support for the anti-tumor uses of silibinin.

Tunable topological slow-light in gyromagnetic photonic crystal waveguides with unified magnetic field.

We have proposed a tunable topological slow-light in a photonic crystal (PC) waveguide with unified magnetic field. The waveguide is constructed by bringing close two gyromagnetic photonic crystals (GPCs) with different structural parameters and introducing a row of Al2O3 rods as the coupling layer. The two GPCs are applied with a unified external static magnetic field (ESMF) instead of two opposite ESMFs. Such waveguide supports a slow-light state originated from the coupling effect of two one-way edge states on both sides of the waveguide. By simply changing the strength of ESMF, one can achieve a tunable slow-light state with large normalized delay-bandwidth product (NDBP) (0.36< NDBP <0.84). Based on these excellent properties, we further design an optical delayer with a compact structure and expansibility simultaneously. This unique topological slow-light state with simple unified magnetic condition, high maneuverability and strong immunity to defects holds promise for many fields such as signal processing, optical modulation, and the design of various slow-light devices.

Recruitment of phosphatase PP2A by RACK1 adaptor protein deactivates transcription factor IRF3 and limits type I interferon signaling.

The transcription factor IRF3 is a central regulator of type I interferon (IFN) signaling. The mechanisms underlying deactivation of IRF3 are poorly understood although many studies suggest that IRF3 activity is terminated through degradation after viral infection. Here we report that IRF3 is deactivated via dephosphorylation mediated by the serine and threonine phosphatase PP2A and its adaptor protein RACK1. The PP2A-RACK1 complex negatively regulated the IRF3 pathway after LPS or poly(I:C) stimulation or Sendai virus (SeV) infection. After challenge with LPS, poly(I:C), or low-titer SeV, activated IRF3 was dephosphorylated and returned to resting state without being degraded, although high-titer SeV infection triggered the degradation of IRF3. Furthermore, PP2A-deficient macrophages showed enhanced type I IFN signaling upon LPS, poly(I:C), and SeV challenge and protected mice from lethal vesicular stomatitis virus infection. Therefore, dephosphorylation of IRF3 is a deactivation mechanism that contributes to termination of IRF3-type I IFN signaling.

Strong Thermo-tolerant Silicone-Modified Waterborne Polyurethane/Polyimide Pressure-Sensitive Adhesive.

A waterborne polyurethane pressure-sensitive adhesive (WPUPSA) has the advantages of low pollution and good viscoelasticity. However, its poor thermo-tolerance limits its application in the field of high temperatures. Hence, a novel silicone-modified strong thermo-tolerant waterborne polyurethane/polyimide pressure-sensitive adhesive is developed as a way to remedy this problem. The single-chain structure of waterborne polyurethane (WPU) is transformed into a network structure by introducing the three-position network structure to increase the cohesive energy and heat resistance of the WPUPSA. Meanwhile, the primary chain of waterborne polyurethane (WPU) is modified by the reaction between pyromellitic dianhydride (PMDA) and isophorone diisocyanate (IPDI) to include an imide ring and a benzene ring with more stable structures and heat resistance. Characterization results of the prepared WPUPSA show that the thermo-tolerance index of the WPUPSA increases by 15.2% and the room temperature 180° peel strength and shear resistance of the WPUPSA increase by 80.9 and 231.8%, respectively. Meanwhile, the temperature corresponding to the maximum thermal decomposition rate of the samples is improved. More importantly, at 80 and 100 °C, the 180° peel strength and shear resistance of the modified samples are stronger than those of the unmodified samples. In addition, the energy storage modulus of WPUPSAs is also greater than the loss and increases with the increase of the frequency. Viscoelasticity dominates in the samples. This will provide new insight for the development of WPUPSAs in the field of high-temperature resistance.

Formic Acid Dehydrogenation through Ligand Design Strategy of Amidation in Half-Sandwich Ir Complexes.

A series of Cp*Ir (Cp* = pentamethylcyclopentadienyl) complexes with amidated 8-aminoquinoline ligands were synthesized and tested for formic acid (FA) dehydrogenation. These complexes showed improved activities compared to pristine 8-anminquinoline (L1). Specially, amidation changed the outer coordination sphere of the complex (3) bearing N-8-quinolinylformamide (L3), and 3 was proved to be a proton-responsive catalyst. Our experimental results and DFT calculations demonstrated that the deprotonated carbanion in L3 could interact with a water molecule to stabilize the transition states and lower the reaction energy barrier, which improved the reaction activity. A turnover frequency of 206250 h-1 was achieved by 3 under optimized conditions. This study presents a method to develop new ligands and modify the existing ligands for efficient FA dehydrogenation.

Phakellisins A-E, cyclopeptides from a marine sponge Phakellia sp. guided by LC-MS.

A chemical investigation of the marine sponge Phakellia sp. from the South China Sea yielded five new cyclopeptides, phakellisins A-E (1-5). Structures of these compounds were determined by comprehensive analysis of 1D/2D NMR, HRESIMS/MS spectroscopic data and the advanced Marfey's method. All compounds were evaluated for their cytotoxic activity. Compound 1 showed a strong inhibitory activity against WSU-DLCL-2 cells with an IC50 value of 5.25 ± 0.2 µM by induction of G0/G1 cell cycle arrest and apoptosis.

Genetic origin of sporadic cases and RNA toxicity in neuronal intranuclear inclusion disease.

BACKGROUND: GGC repeat expansion in NOTCH2NLC has been recently linked to neuronal intranuclear inclusion disease (NIID) via unknown disease mechanisms. Herein, we explore the genetic origin of the sporadic cases and toxic RNA gain-of-function mechanism in NIID. METHODS: Multiple genetic screenings were performed on NIID individuals and their available family members. Methylation status of blood DNA, NOTCH2NLC mRNA level from muscle biopsies and RNA foci from skin biopsies of NIID individuals or asymptomatic carriers were evaluated and compared. RESULTS: In two sporadic NIID families, we identified two clinically and pathologically asymptomatic fathers carrying large GGC repeat expansion, above 300 repeats, with offspring repeat numbers of 172 and 148, respectively. Further evaluation revealed that the GGC repeat numbers in the sperm from two asymptomatic fathers were only 63 and 98, respectively. The CpG island in NOTCH2NLC of the asymptomatic carriers was hypermethylated, and accordingly, the NOTCH2NLC mRNA levels were decreased in the asymptomatic fathers. GGC repeat expansion RNA formed RNA foci and sequestered RNA binding proteins into p62 positive intranuclear inclusions in NIID individuals but not in the control or asymptomatic carrier. CONCLUSION: Our study suggested the GGC repeat expansion in NOTCH2NLC might have a disease-causing number ranging from ~41 to ~300 repeats. The contraction of GGC repeat expansion in sperm could be a possible mechanism for the paternal-biased origin in some sporadic or recessive inherited NIID individuals. The toxic RNA gain-of-function mechanism was identified to be involved in the pathogenicity of this disease.

Transurethral en bloc resection of a bladder perivascular epithelioid cell tumor (PEComa): a case report.

BACKGROUND: Perivascular epithelioid cell tumor (PEComa) is a mesenchymal tumor with distinct histologic and immunologic features. PEComas that originate in the bladder are extremely rare clinically, with only 35 cases reported in the English literature thus far. Here, we report a case of bladder PEComa resection by transurethral en bloc resection of bladder tumor (ERBT). CASE PRESENTATION: A 66-year-old female with a history of poorly controlled type 2 diabetes with associated complications of frequent urinary tract infections presented to our hospital for a routine physical examination. Outpatient ultrasound examination revealed a strong echogenic mass of approximately 1.5 × 1.3 × 1.3 cm in size on the posterior wall of the bladder. The enhanced computed tomography and enhanced magnetic resonance imaging after admission both suggested a well-defined isolated nodular mass on the posterior wall of the bladder with significant enhancement on the enhanced scan. The tumor was successfully and completely resected by ERBT. Postoperative pathological examination and immunohistochemical results confirmed the mass was a bladder PEComa. No tumor recurrence was observed in the six-month postoperative follow-up. CONCLUSION: Bladder PEComa is an extremely rare mesenchymal tumor of the urinary system. When imaging and cystoscopy reveal a nodular mass with an abundant blood supply in the bladder, PEComa should be included in the differential diagnosis of bladder tumors. Surgical resection is currently the primary option for the treatment of bladder PEComa. For a solitary, pedunculated, narrow-based, small-sized bladder PEComa, resection of the tumor by ERBT was a safe and feasible approach in our patient and may be considered for similar cases in the future.

LC-MS Analysis of Ginsenosides in Different Parts of Panax quinquefolius and Their Potential for Coronary Disease Improvement.

Seven main ginsenosides, including ginsenoside Re, ginsenoside Rb1, pseudoginsenoside F11, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd, and ginsenoside F2, were identified by LC-QTOF MS/MS from root, leaf and flower extracts of Panax quinquefolius. These extracts promoted intersegmental vessel growth in a zebrafish model, indicating their potential cardiovascular health benefits. Network pharmacology analysis was then conducted to reveal the potential mechanisms of ginsenoside activity in the treatment of coronary artery disease. GO and KEGG enrichment analyses elucidated that G protein-coupled receptors played a critical role in VEGF-mediated signal transduction and that the molecular pathways associated with ginsenoside activity are involved in neuroactive ligand-receptor interaction, cholesterol metabolism, the cGMP-PKG signaling pathway, etc. Moreover, VEGF, FGF2, and STAT3 were confirmed as the major targets inducing proliferation of endothelial cells and driving the pro-angiogenic process. Overall, ginsenosides could be potent nutraceutical agents that act to reduce the risks of cardiovascular disease. Our findings will provide a basis to utilize the whole P. quinquefolius plant in drugs and functional foods.

Research and Implementation of Millet Ear Detection Method Based on Lightweight YOLOv5.

As the millet ears are dense, small in size, and serious occlusion in the complex grain field scene, the target detection model suitable for this environment requires high computing power, and it is difficult to deploy the real-time detection of millet ears on mobile devices. A lightweight real-time detection method for millet ears is based on YOLOv5. First, the YOLOv5s model is improved by replacing the YOLOv5s backbone feature extraction network with the MobilenetV3 lightweight model to reduce model size. Then, using the multi-feature fusion detection structure, the micro-scale detection layer is augmented to reduce high-level feature maps and low-level feature maps. The Merge-NMS technique is used in post-processing for target information loss to reduce the influence of boundary blur on the detection effect and increase the detection accuracy of small and obstructed targets. Finally, the models reconstructed by different improved methods are trained and tested on the self-built millet ear data set. The AP value of the improved model in this study reaches 97.78%, F1-score is 94.20%, and the model size is only 7.56 MB, which is 53.28% of the standard YoloV5s model size, and has a better detection speed. Compared with other classical target detection models, it shows strong robustness and generalization ability. The lightweight model performs better in the detection of pictures and videos in the Jetson Nano. The results show that the improved lightweight YOLOv5 millet detection model in this study can overcome the influence of complex environments, and significantly improve the detection effect of millet under dense distribution and occlusion conditions. The millet detection model is deployed on the Jetson Nano, and the millet detection system is implemented based on the PyQt5 framework. The detection accuracy and detection speed of the millet detection system can meet the actual needs of intelligent agricultural machinery equipment and has a good application prospect.

Evaluating the effects of formation and stabilization of opal/sand aggregates with organic matter amendments.

Opal (SiO2·nH2O, amorphous silica), the by-product of alumina extraction from coal fly ash (CFA), has a strong adsorption capacity and is also an important component of clay minerals in soils. The combining of opal with sand to form artificial soils is an effective disposal strategy for large-scale CFA stockpiles and reduction of environmental risk. Nevertheless, its poor physical condition limits plant growth. Organic matter (OM) amendments have broad potential applications for water-holding and improving soil aggregation. Effects of OMs (vermicompost (VC), bagasse (BA), biochar (BC) and humic acid (HA)) on the formation, stability and pore characteristics of opal/sand aggregates were evaluated through 60-day laboratory incubation experiments. Results demonstrated that four OMs could reduce pH, with BC having the most significant effect, VC significantly increasing the electrical conductivity (EC) and TOC content of the aggregates. Except for HA, other OMs could improve the aggregates' water-holding capacity. The mean weight diameter (MWD) and percentage of >0.25 mm aggregates (R0.25) of BA-treated aggregates were the largest, and BA had the most noticeable contribution to macro-aggregate's formation. The best aggregate stability was obtained with HA treatment, meanwhile the percentage of aggregate destruction (PAD0.25) decreased with the addition of HA. After amendments, the proportion of organic functional groups increased, which favored aggregate's formation and stability; the surface pore characteristics were improved, with the porosity ranging from 70% to 75%, reaching the level of well-structured soil. Overall, the addition of VC and HA can effectively promote aggregates' formation and stabilization. This research may play a key role in converting CFA or opal into artificial soil. The combining of opal with sand to form artificial soil will not only solve the environmental problems caused by large-scale CFA stockpiles but will also enable the comprehensive utilization of siliceous materials in agriculture.

Antibacterial and Alkali-responsive Cationic Waterborne Polyurethane Based on Modification of Aloe Emodin.

Cationic water-based polyurethane(CWPU) was synthesized to explore aloe-emodin modifies to obtain CWPU materials with better comprehensive performance. It provides a simple way to synthesize antibacterial waterborne polyurethane, which is to introduce the end-blocking group of herbal extracts into the structure. It contains synergistic antibacterial effect of herbal antibacterial and quaternary ammonium ion on Escherichia coli. It makes the material resist the erosion of bacterial, and increase the service life of materials. When the pH value of the environment changes, the UV absorbance of the aloe-emodin modified cationic water-based polyurethane(AE-CWPU) also changes. Therefore, within a certain detection range, AE-CWPU has great applications in the field of smart response materials. The modified thermodynamic properties have been improved, and the mechanical properties basically maintained the maximum stress, and the elongation at break was reduced.

Identification of COPA as a potential prognostic biomarker and pharmacological intervention target of cervical cancer by quantitative proteomics and experimental verification.

BACKGROUND: Cervical cancer is the most fatal gynecological carcinoma in the world. It is urgent to explore novel prognostic biomarkers and intervention targets for cervical cancer. METHODS: Through integrated quantitative proteomic strategy, we investigated the protein expression profiles of cervical cancer; 28 fresh frozen tissue samples (11 adenocarcinoma (AC), 12 squamous cell carcinoma (SCC) and 5 normal cervixes (HC)) were included in discover cohort; 45 fresh frozen tissue samples (19 AC, 18 SCC and 8 HC) were included in verification cohort; 140 paraffin-embedded tissues samples of cervical cancer (85 AC and 55 SCC) were used for immunohistochemical evaluation (IHC) of coatomer protein subunit alpha (COPA) as a prognostic biomarker for cervical cancer; how deficiency of COPA affects cell viability and tumorigenic ability of cervical cancer cells (SiHa cells and HeLa cells) were evaluated by cell counting kit-8 and clone formation in vitro. RESULTS: We identified COPA is a potential prognostic biomarker for cervical cancer in quantitative proteomics analysis. By retrospective IHC analysis, we additionally verified the proteomics results and demonstrated moderate or strong IHC staining for COPA is an unfavourable independent prognostic factor for cervical cancer. We also identified COPA is a potential pharmacological intervention target of cervical cancer by a series of in vitro experiments. CONCLUSION: This study is the first to demonstrate that COPA may contribute to progression of cervical cancer. It can serve as a potential prognostic biomarker and promising intervention target for cervical cancer.

Proposal to transfer Bacillus lacisalsi Dong et al. 2021 to the genus Alteribacter as Alteribacter lacisalsi comb. nov.

In the present study, the taxonomic position of Bacillus lacisalsi YSP-3T was evaluated using phylogenetic and genome-based comparison. B. lacisalsi YSP-3T showed the highest 16S rRNA gene sequence similarity to Alteribacter natronophilus M30T (98.4 %), followed by Alteribacter aurantiacus K1-5T (97.5 %) and Alteribacter populi FJAT-45347T (97.2 %). In phylogenetic (based on 16S rRNA gene sequences) and phylogenomic (based on 71 bacterial single-copy genes) trees, B. lacisalsi YSP-3T clustered with the members of the genus Alteribacter. The amino acid identity (AAI) values between B. lacisalsi YSP-3T and the members of the genus Alteribacter were >65 %, which is above the cut-off level (65-95 %) for genus delineation. The average nucleotide identity (ANI) values between B. lacisalsi YSP-3T and the members of the genus Alteribacter were <95 %, which is lower than the threshold value (95-96 %) for bacterial species delineation. The AAI value suggested that B. lacisalsi YSP-3T was a member of the genus Alteribacter while the ANIb value suggested it as a novel species of the genus Alteribacter. Based on the results, we propose to transfer Bacillus lacisalsi to the genus Alteribacter as Alteribacter lacisalsi comb. nov.

Prophylactic Effect of Simultaneous Placement of Mesh on Incidence of Parastomal Hernia After Miles' Surgical Resection of Colorectal Cancer: A Prospective Study.

INTRODUCTION: To assess the prophylactic effect of simultaneous placement of mesh and the incidence of parastomal hernia (PSH) after abdominoperineal resection of rectal cancer. METHODS: This study included real-world data of 56 surgically resected patients with colorectal cancer who were consecutively assigned to two groups: control (no mesh, n = 32) and experimental (received mesh, n = 24). An artificial patch was placed under the tunica vaginalis of rectus abdominis for patients in the experimental group, whereas those in the control group received routine sigmoidostomy. The median follow-up time was >20 mo. The difference in hazards function was analyzed by cox regression analysis. The Kaplan-Meir analysis was used to determine the survival curves. A P value of <0.05 was considered as significant. RESULTS: The postoperative incidence rate of PSH was lower in the experimental (41.7%) group than in the control group (71.9%; P = 0.045). The PSH postoperative time in the experimental group was significantly delayed compared to the control group (48 mo versus 10 mo; P < 0.001). The risk of progression from H1 to H2 was less in the experimental group compared to the control group (49.28% versus 60.86%; P = 0.14). CONCLUSIONS: Prophylactic mesh placement significantly prolonged postoperative time for the recurrence of PSH. The incidence of recurrence of H2 (severe PSH) requiring secondary surgical repair was also reduced.

Citrus Huanglongbing detection and semi-quantification of the carbohydrate concentration based on micro-FTIR spectroscopy.

Citrus Huanglongbing (HLB) is nowadays one of the most fatal citrus diseases worldwide. Once the citrus tree is infected by the HLB disease, the biochemistry of the phloem region in midribs would change. In order to investigate the carbohydrate changes in phloem region of citrus midrib, the semi-quantification models were established to predict the carbohydrate concentration in it based on Fourier transform infrared microscopy (micro-FTIR) spectroscopy coupled with chemometrics. Healthy, asymptomatic-HLB, symptomatic-HLB, and nutrient-deficient citrus midribs were collected in this study. The results showed that the intensity of the characteristic peak varied with the carbohydrate (starch and soluble sugar) concentration in citrus midrib, especially at the fingerprint regions of 1175-900 cm-1, 1500-1175 cm-1, and 1800-1500 cm-1. Furthermore, semi-quantitative prediction models of starch and soluble sugar were established using the full micro-FTIR spectra and selected characteristic wavebands. The least squares support vector machine regression (LS-SVR) model combined with the random frog (RF) algorithm achieved the best prediction result with the determination coefficient of prediction ([Formula: see text]) of 0.85, the root mean square error of prediction (RMSEP) of 0.36%, residual predictive deviation (RPD) of 2.54, and [Formula: see text] of 0.87, RMSEP of 0.37%, RPD of 2.76, for starch and soluble sugar concentration prediction, respectively. In addition, multi-layer perceptron (MLP) classification models were established to identify HLB disease, achieving the overall classification accuracy of 94% and 87%, based on the full-range spectra and the optimal wavenumbers selected by the random frog (RF) algorithm, respectively. The results demonstrated that micro-FTIR spectroscopy can be a valuable tool for the prediction of carbohydrate concentration in citrus midribs and the detection of HLB disease, which would provide useful guidelines to detect citrus HLB disease.