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1.
Zhonghua Fu Chan Ke Za Zhi ; 59(2): 135-142, 2024 Feb 25.
Artigo em Zh | MEDLINE | ID: mdl-38389233

RESUMO

Objective: To analyze and summarize the oncological outcomes after laparoscopic radical trachelectomy (LRT) for early stage cervical cancer. Methods: The clinical data and follow-up results of 148 patients with early stage cervical cancer who underwent LRT in Renji Hospital, School of Medicine, Shanghai Jiao Tong University from July 2014 to June 2023 were collected, while tumor outcomes and postoperative pregnancy were analyzed retrospectively. Results: (1) General situation: the median age of 148 patients with LRT was 33 years (range: 19-42 years). Pathological type: 111 cases of squamous cell carcinoma, 36 cases of adenocarcinoma, 1 case of adenosquamous carcinoma. International Federation of Gynecology and Obstetrics (2018) stage: 17 cases of stage Ⅰa1 with lympho-vascular space invasion, 25 cases of stage Ⅰa2, 102 cases of stage Ⅰb1, and 4 cases of stage Ⅰb2. (2) Tumor outcomes: 148 patients were followed up regularly after LRT, and the median follow-up time was 59 months (range: 2-104 months). During the follow-up period, 5 cases of tumor recurred (including 1 death), and the median recurrence time was 10 months (range: 4-33 months). Among them, there were 3 cases of pelvic metastasis, 1 case of distant metastasis, and 1 case of both pelvic and distant metastasis. Both 3-year and 5-year disease-free survival rates of 148 patients were 94.5%, and the 5-year overall survival rate was 98.9%. (3) Postoperative pregnancy: among 148 patients with LRT, 67 patients had pregnancy requirements, followed up for 1 year, and 20 of them were pregnant, with a pregnancy rate of 29.9% (20/67). Among the 20 pregnant patients, 2 cases early abortion, 1 case mid-term abortion, and 17 cases gave birth (including 4 cases of premature birth and 13 cases of full-term birth). Conclusion: Under the condition of strict control of surgical indications, guaranteed surgical scope and tumor-free operation, LRT in patients with early cervical cancer has a good outcome.


Assuntos
Laparoscopia , Traquelectomia , Neoplasias do Colo do Útero , Gravidez , Feminino , Humanos , Adulto Jovem , Adulto , Neoplasias do Colo do Útero/patologia , Traquelectomia/métodos , Resultado do Tratamento , Estudos Retrospectivos , Estadiamento de Neoplasias , China , Laparoscopia/métodos
2.
Toxicol Appl Pharmacol ; 434: 115799, 2022 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-34798142

RESUMO

Arsenic is a naturally occurring element present in food, soil and water and human exposure is associated with increased cancer risk. Arsenic inhibits DNA repair at low, non-cytotoxic concentrations and amplifies the mutagenic and carcinogenic impact of other DNA-damaging agents, such as ultraviolet radiation (UVR). Arsenic exposure leads to oxidation of zinc coordinating cysteine residues, zinc loss and decreased activity of the DNA repair protein poly(ADP)ribose polymerase (PARP)-1. Because arsenic stimulates NADPH oxidase (NOX) activity leading to generation of reactive oxygen species (ROS), the goal of this study was to investigate the role of NOX in arsenic-induced inhibition of PARP activity and retention of DNA damage. NOX involvement in the arsenic response was assessed in vitro and in vivo. Keratinocytes were treated with or without arsenite, solar-simulated UVR, NOX inhibitors and/or isoform specific NOX siRNA. Knockdown or inhibition of NOX decreased arsenite-induced ROS, PARP-1 oxidation and DNA damage retention, while restoring arsenite inhibition of PARP-1 activity. The NOX2 isoform was determined to be the major contributor to arsenite-induced ROS generation and DNA damage retention. In vivo DNA damage was measured by immunohistochemical staining and analysis of dorsal epidermis sections from C57BI/6 and p91phox knockout (NOX2-/-) mice. There was no significant difference in solar-simulated UVR DNA damage as detected by percent PH2AX positive cells within NOX2-/- mice versus control. In contrast, arsenite-dependent retention of UVR-induced DNA damage was markedly reduced. Altogether, the in vitro and in vivo findings indicate that NOX is involved in arsenic enhancement of UVR-induced DNA damage.


Assuntos
Arsênio/toxicidade , Dano ao DNA/efeitos dos fármacos , Dano ao DNA/efeitos da radiação , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , NADPH Oxidase 2/metabolismo , Raios Ultravioleta , Animais , Linhagem Celular , Humanos , Queratinócitos/efeitos dos fármacos , Queratinócitos/efeitos da radiação , Camundongos , Camundongos Knockout , NADPH Oxidase 1/genética , NADPH Oxidase 1/metabolismo , NADPH Oxidase 2/genética , Espécies Reativas de Oxigênio
3.
Artigo em Zh | MEDLINE | ID: mdl-32892585

RESUMO

Objective: To understand the status quo of nurses' professional mission and explore the influencing factors of nurses' professional mission. Methods: From November to December, 2018, 316 nurses from three tertiary hospitals in Tianjin were selected as the research objects. The occupational mission scale was used to investigate the sense of professional mission, the nursing work environment scale was used for the nursing work environment survey, and the work family conflict scale was used for the work family conflict investigation. Pearson correlation analysis was used to analyze the correlation among nurses' sense of professional mission, nursing work environment and work family conflict; multiple linear regression analysis was used to analyze the influencing factors of nurses' sense of professional mission. Results: The score of professional mission of nurses was (2.90±0.56) . Average monthly income, nursing work environment and work family conflict were the influencing factors of nurses' professional mission (P<0.05) . The results of hierarchical regression showed that the higher the average monthly income (ß=0.252) , the higher the sense of professional mission of nurses (R(2)=0.064) ; after controlling general data, the two dimensions of nursing work environment: Nurses' participation in hospital affairs (ß=0.263) , high-quality nursing service foundation (ß=0.368) , and work family conflict (ß=-0.145) could explain 43.1% of the total variation of professional mission. Conclusion: The sense of professional mission of nurses is above the middle level. Nursing managers should start with the influencing factors such as average monthly income, nursing working environment and work family conflict, so as to stimulate or improve nurses' sense of professional mission.


Assuntos
Recursos Humanos de Enfermagem Hospitalar , Estudos Transversais , Hospitais , Humanos , Satisfação no Emprego , Inquéritos e Questionários , Local de Trabalho
4.
Zhonghua Gan Zang Bing Za Zhi ; 25(2): 111-114, 2017 Feb 20.
Artigo em Zh | MEDLINE | ID: mdl-28297796

RESUMO

Liver cancer is a common malignant tumor. Although the overall incidence and mortality rates of liver cancer has been decreasing in recent years, the incidence of nonalcoholic fatty liver disease (NAFLD)-associated liver cancer tends to increase. The health and social issues brought by NAFLD-associated liver cancer have attracted more and more attention, and its epidemiology, pathogenesis, and diagnosis and treatment await further research. This article summarizes the current epidemiology and pathogenesis of NAFLD-associated liver cancer.


Assuntos
Neoplasias Hepáticas/epidemiologia , Neoplasias Hepáticas/fisiopatologia , Hepatopatia Gordurosa não Alcoólica/epidemiologia , Hepatopatia Gordurosa não Alcoólica/fisiopatologia , Humanos , Incidência
5.
Zhonghua Fu Chan Ke Za Zhi ; 51(6): 442-7, 2016 Jun 25.
Artigo em Zh | MEDLINE | ID: mdl-27356480

RESUMO

OBJECTIVE: To explore the outcomes of the oncology, the fertility and pregnancy on patients with early-stage cervical cancer after undergoing fertility-sparing treatments, and to investigate its value on clinical treatment. METHODS: A total of 29 patients with cervical cancer (stage Ⅰa2-Ⅰb1) who had undergone the fertility- sparing treatments in Renji Hospital, School of Medicine, Shanghai Jiaotong University from February 2010 to September 2015 were analyzed retrospectively. Surgical procedures included transvaginal cervical cold knife conization (CKC) + laparoscopic pelvic lymphadenectomy (PLD) or total laparoscopic uterine cervix extensive resection (RT; contains PLD); 48 patients with early cervical cancer under going laparoscopic uterine extensive resection (RH; contains PLD) by the same group of physicians in the same period were chosen as the control group. The perioperative related indicators, postoperative specimen examination, pregnancies after surgery and postoperative tumor follow- up results were retrospectively analyzed and compared between the two groups. RESULTS: (1) Among these 29 patients in the study group, 26 cases were underwent the fertility-sparing surgery quick frozen pathological examinations were positive in 3 cases, which underwent total laparoscopic RT eventually. (2) The perioperative related indicators: compared with the study group and the control group, the operation time [(182±21), (147±24) minutes, respectively] has significant difference (t=6.563, P<0.01). There were no significant difference (P> 0.05) in intra-operative blood loss [(102±26), (99±34) ml], postoperative aerofluxus time [(1.3±0.4), (1.1±0.9) days], the average length of hospital stay [(11.2±2.1), (10.6±3.5) days], rate of urine retention [10%(3/29), 10%(5/48)] and rate of postoperative infection [3% (1/29), 2%(1/48)]. (3)Postoperative specimen examination: compared with the study group and the control group, there were no significant difference (P>0.05) innumber of removed lymphatic nodes (23.4±4.1, 22.8±3.9), length of cardinal ligament [(2.9±0.5), (3.0±0.6) cm], lengthof uterosacral ligament [(2.6±0.7), (2.8±0.4) cm], length of removed vaginal [(3.4±0.3), (3.5±0.3) cm]. (4) Pregnancies after surgery and postoperative tumor follow-up results: in the study group, only 14 patients had fertility requirement after treatments. Pregnancies occurred in 5 women (5/14), which included1 case of full-term labor, 1 case of preterm labor, and 3 cases of spontaneous abortion. The Average follow-up time in postoperative patients of the study group and control group was 29.4, and 30.2 months respectively. In follow- up period, compare with study group and the control group, there was no significant difference (χ(2)=0.004, P> 0.05) in the recurrence rate [4% (1/26), 4% (2/48)]. CONCLUSION: Fertility-sparing surgery of early-stage cervical cancer is safe but the outcome of the fertility and pregnancy is still need toimprove.


Assuntos
Conização/métodos , Preservação da Fertilidade , Histerectomia/métodos , Laparoscopia , Excisão de Linfonodo/métodos , Neoplasias do Colo do Útero/cirurgia , Aborto Espontâneo/epidemiologia , Adulto , China/epidemiologia , Feminino , Humanos , Recidiva Local de Neoplasia , Estadiamento de Neoplasias , Trabalho de Parto Prematuro/epidemiologia , Gravidez , Resultado da Gravidez , Estudos Retrospectivos , Resultado do Tratamento , Neoplasias do Colo do Útero/epidemiologia , Adulto Jovem
7.
Br J Cancer ; 109(11): 2778-82, 2013 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-24169350

RESUMO

BACKGROUND: There are limited data comparing the prognosis and fertility outcomes of the patients with early cervical cancer treated by trans-vaginal radical trachelectomy (VRT) or abdominal radical trachelectomy (ART).The objective of this study was to compare the surgical and pathologic characteristics, the prognosis and fertility outcomes of the patients treated by VRT or ART. METHODS: Matched-case study based on a prospectively maintained database of patients underwent radical trachelectomy in 10 centres of China was designed to compare the prognosis and fertility outcomes of the patients treated by VRT or ART. RESULTS: Totally 150 cases, 77 in the VRT and 73 in the ART group, were included. VRT and ART provide similar surgical and pathological outcomes except larger specimens obtained by ART. In the ART group, no patient developed recurrent diseases, but, in the VRT group, 7 (9.8%) patients developed recurrent diseases and 2 (1.6%) patients died of the tumours (P=0.035). The rate of pregnancy in the VRT group was significantly higher than those of ART (39.5% vs 8.8%; P=0.003). The patients with tumour size >2 cm showed significant higher recurrent rate (11.6% vs 2.4%, P<0.05) and lower pregnant rate (12.5% vs 32.1%, P=0.094) compared with the patients with tumour size <2 cm. CONCLUSION: Patients treated by ART obtained better oncology results, but their fertility outcomes were unfavourable compared with VRT. Tumour size <2 cm should be emphasised as an indication for radical trachelectomy for improving the outcome of fertility and prognosis.


Assuntos
Abdome/cirurgia , Carcinoma de Células Escamosas/cirurgia , Histerectomia/métodos , Neoplasias do Colo do Útero/cirurgia , Vagina/cirurgia , Abdome/patologia , Adolescente , Adulto , Carcinoma de Células Escamosas/epidemiologia , Carcinoma de Células Escamosas/patologia , China/epidemiologia , Feminino , Humanos , Histerectomia/efeitos adversos , Estadiamento de Neoplasias , Gravidez , Taxa de Gravidez , Neoplasias do Colo do Útero/epidemiologia , Neoplasias do Colo do Útero/patologia , Vagina/patologia , Adulto Jovem
8.
Plant Dis ; 97(12): 1656, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30716816

RESUMO

Gray leaf spot of maize (Zea mays L.) is an important foliar disease in many parts of China. The causal organism of gray leaf spot in China is generally regarded as Cercospora zeae-maydis (3). In October 2011, symptoms similar to gray leaf spot were observed on 77% of maize plants in 25 locations (about 3,000 ha.) of Yunnan Province, China, and the disease could cause yield losses of 35 to 50%. The symptoms of leaf spot were different from those caused by C. zeae-maydis. The lesions on leaves were oblong, pale gray to pale brown, 2 to 3 × 5 to 40 mm, and confined by leaf veins that eventually coalesced. To identify the pathogen, 75 leaf samples were collected from 25 fields (three leaf samples for each field) at the kernel maturity stage. Single, well-separated lesions were excised and surface-sterilized by placing them in 75% ethanol for 5 s, then disinfested in 2% sodium hypochlorite for 5 min and rinsed with sterilized water. The lesions were incubated on water agar (WA) at 24°C for 48 to 72 h to allow sporulation. Seventy-five single-conidial isolates were obtained and cultured as described in Crous (1). Morphology of the isolates was determined on plates containing maize leaf powder agar (MLPA). After 5 days, isolates produced pale brown mycelia that consisted of 3- to 4-µm-wide, septate, branched hyphae. Conidiophores were 5 to 7 × 55 to 100 µm, straight to slightly flexuous, and usually 1- to 5-septate. Conidia were average 7.5 × 68 µm, fusiform, apex subobtuse, base subtruncate, and 3- to 6-septate. These characteristics are similar to C. zeina (2). The internal transcribed spacer (ITS) region of rDNA was amplified from each of the 75 isolates using primers ITS1/ITS4 and sequenced. The same sequences were obtained and the sequence of isolate YNGLS was submitted to GenBank (Accession No. KC878692). BLAST analysis of the sequence showed 100% confirmation to C. zeina (DQ185081). Additionally, a PCR-based diagnostic test using species-specific primers (2) confirmed the identification of the 75 isolates as C. zeina. The pathogenicity of the isolates was tested on greenhouse grown maize variety Huidan 4. The test was performed on 40 plants and replicated three times. The plants were inoculated at the 10 leaf stage by injecting 2 ml of conidial suspensions (2,500 conidia ml-1) into leaf whorl using a hypodermic syringe, and control plants were injected with sterile water. Conidia were collected from 5-day-old cultures grown on MLPA and suspended in sterile water. Forty days after inoculation, all inoculated plants showed characteristic lesions on leaves, but control plants remained asymptomatic. C. zeina was reisolated from the lesions, and the identity of the reisolates was confirmed by the morphological and molecular characteristics as stated above. C. zeina was previously reported as the causal agent of maize gray leaf spot (2). To our knowledge, this is the first report of C. zeina causing gray leaf spot of maize in China. References: (1) P. W. Crous. Mycologia Memoir. 21:1, 1998. (2) P. W. Crous et al. Stud. Mycol. 55:189, 2006. (3) C. H. Lu et al. J. Southwest China Normal Univ. 37:51, 2012.

9.
J Anim Physiol Anim Nutr (Berl) ; 95(3): 359-67, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21039925

RESUMO

The influence of a high-protein [HP, 47% of metabolizable energy (ME)] diet on energy balance was evaluated in obese cats allowed ad libitum access to food. Energy intake, body weight, body composition, energy expenditure, and concentrations of hormones and metabolites associated with carbohydrate and lipid metabolism (glucose, insulin, free fatty acids, triglycerides and leptin) were measured in cats after consuming either a moderate protein (MP, 27% of ME) or HP diet for 4 months. Indirect respiration calorimetry showed that resting and total energy expenditure (kJ/day) adjusted for either body weight or lean body mass was increased in cats consuming the HP in relation to MP diets. However, voluntary energy intake also was increased in the HP treatment and, thus, there was no difference in body weight between animals consuming the two diets. Body composition measurements using deuterium oxide dilution showed that dietary protein content did not alter amounts of either lean body mass or fat mass. No significant differences (p > 0.05) were observed between the two treatment groups for blood glucose, free fatty acid or leptin concentrations, although there was a trend (p = 0.054) towards an increase of serum insulin concentrations in the cats eating the HP diet. This study showed that short-term ad libitum feeding of an HP diet did not reduce food intake or promote weight loss in obese cats. However, energy expenditure was increased in the HP diet group and it is possible that this effect of HP might help promote weight loss when energy intake is restricted.


Assuntos
Ração Animal/análise , Doenças do Gato/dietoterapia , Dieta/veterinária , Proteínas Alimentares/farmacologia , Metabolismo Energético/fisiologia , Obesidade/veterinária , Criação de Animais Domésticos , Fenômenos Fisiológicos da Nutrição Animal , Animais , Calorimetria Indireta/veterinária , Gatos , Feminino , Masculino
10.
11.
J Exp Med ; 176(4): 1025-31, 1992 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-1402648

RESUMO

By generating phosphorylcholine (PC)-specific, wild-type (mu), and chimeric (mu-I-A alpha) antigen receptor transfectants of mature B cells, we have shown that the COOH terminus of the mu heavy chain is essential for three major functions: immediate signal transduction (measured as changes in intracellular Ca2+), antigen presentation, and induction of immunoglobulin M secretion. A more detailed analysis of structural requirements of the COOH-terminal domains contributing to these functions was achieved by systematically replacing the spacer, cytoplasmic, and transmembranal domains of the mu-I-A alpha chimeric chain with those of mu. Using this rescue approach, we show that the carboxyl two-thirds of the transmembranal domain (proximal to the cytoplasmic domain) is required for induction of intracellular Ca2+, whereas the complete transmembranal domain is required for the function of antigen presentation but is dispensable for induction of antibody secretion.


Assuntos
Linfócitos B/imunologia , Fosforilcolina/imunologia , Receptores de Antígenos de Linfócitos B/fisiologia , Sequência de Aminoácidos , Animais , Cálcio/metabolismo , Linhagem Celular , Membrana Celular/imunologia , Imunoglobulina M/metabolismo , Linfoma de Células B , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Mutagênese Sítio-Dirigida , Receptores de Antígenos de Linfócitos B/genética , Transdução de Sinais/imunologia , Relação Estrutura-Atividade , Transfecção
12.
Zhonghua Wei Chang Wai Ke Za Zhi ; 23(4): 384-389, 2020 Apr 25.
Artigo em Zh | MEDLINE | ID: mdl-32306607

RESUMO

Objective: To explore the safety and feasibility of da Vinci robot surgical systems in natural orifice specimen extraction surgery (NOSES) for rectal neoplasms. Methods: A descriptive cohort study was used. Inclusion criteria: (1) age ≥18 years old; (2) diagnosis of rectal cancer by biopsy via colonoscopy or benign neoplasm locating in rectum that could not be resected locally through the anus; (3) R0 resection can be achieved by preoperative evaluation; (4) the CDmax (maximum circumferential diameter) was ≤5 cm or specimens could still be extracted from the anus despite a CDmax exceeding 5 cm but was along the longitudinal axis of the rectum. Exclusion criteria: (1) emergency operation due to gastrointestinal obstruction, perforation, or bleeding; (2) distal metastasis, induding lung, bone, or liver, that could not be resected simultaneously; (3) history of abdominal surgery or any other contraindications for robotic surgery. Clinicopathological data of 162 patients with rectal neoplasms who underwent robotic NOSES at the General Surgery Department of the Second Xiangya Hospital of Central South University from March 2016 to July 2019 were retrospectively collected. Of 162 patients, 94 were male and 68 were female; the average age was (57±13) years; the average BMI was (23.5±3.2) kg/m(2); the average distance from tumor to the anal verge was (8.2±2.9) cm. Five trocars were used to perform total mesorectal excision (TME), and the descending colon artery was preserved. Sterile endoscope sleeve for the specimen extraction was inserted into the pelvic cavity through the anus, and the resected specimen was pulled out through the sleeve. Outcomes of safety (operation time, intraoperative blood loss and postoperative morbidity of complication) and oncological outcomes (number of lymph nodes harvested, rate of lymph node metastasis and rate of positive resection margin) were collected. Results: All the 162 cases completed robotic NOSES successfully with no conversion to laparotomy. The average operation time was (188.7±79.8) minutes; the average blood loss was (47.1±33.2) ml; the average and the maximum CDmax of specimens were (3.4±1.5) cm and 12 cm respectively. A total of 154 patients underwent robotic TME. One underwent robotic TME plus resection of liver metastasis; one underwent robotic TME plus partial transverse colectomy; two patients underwent robotic TME plus ovariectomy; another two underwent robotic TME plus hysterectomy; one patient underwent robotic TME plus left partial nephrectomy due to renal angioleiomyoma; another one underwent robotic TME plus ureteral repair due to intraoperative injury of the left ureter. All the specimens were extracted through the anus. Protective ileostomy was performed in 6.8% (11/162) of the patients. The average number of lymph node harvested was 14.9±5.1. According to pathological reports, 156 neoplasms were adenocarcinoma. Tis stage was 1.3% (2/156), T1 stage was 9.0% (14/156), T2 stage was 26.3% (41/156), T3 stage was 35.9% (56/156), and T4 stage was 27.6% (43/156). Lymph node metastasis accounted for 34.6% (54/156), and simultaneous liver metastasis was observed in one case. Circumferential resection margins (CRMs) and upper and lower resection margins were negative in all the patients. The average postoperative feeding time and postoperative hospital stay were (4.2±4.1) days and (11.4±7.7) days, respectively. Postoperative morbidity of complication was 12.3% (20/162). The incidence of anastomotic leakage was 4.9% (8/162), of which only 4 cases (2.5%) received ileostomy. Within postoperative 90-day, no anal dysfunction or death were found. Conclusion: Robotic NOSES for rectal neoplasms is safe and feasible.


Assuntos
Adenocarcinoma/cirurgia , Cirurgia Endoscópica por Orifício Natural/métodos , Protectomia/métodos , Neoplasias Retais/cirurgia , Procedimentos Cirúrgicos Robóticos , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
13.
IEEE Trans Cybern ; 49(4): 1339-1352, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29994650

RESUMO

Collaborative filtering is widely used in recommendation systems. A user can get high-quality recommendations only when both the user himself/herself and other users actively participate, i.e., provide sufficient ratings. However, due to the rating cost, rational users tend to provide as few ratings as possible. Therefore, there exists a tradeoff between the rating cost and the recommendation quality. In this paper, we model the interactions among users as a game in satisfaction form and study the corresponding equilibrium, namely satisfaction equilibrium (SE). Considering that accumulated ratings are used for generating recommendations, we design a behavior rule which allows users to achieve an SE via iteratively rating items. We theoretically analyze under what conditions an SE can be learned via the behavior rule. Experimental results on Jester and MovieLens data sets confirm the analysis and demonstrate that, if all users have moderate expectations for recommendation quality and satisfied users are willing to provide more ratings, then all users can get satisfying recommendations without providing many ratings. The SE analysis of the proposed game in this paper is helpful for designing mechanisms to encourage user participation.

14.
Equine Vet J ; 51(6): 756-759, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30868638

RESUMO

BACKGROUND: Salmonella enterica subspecies enterica serovar abortus equi (S. abortus equi) is well known as the aetiological agent of equine abortion. However, abortion caused by S. abortus equi has not been previously described in donkeys. OBJECTIVES: To investigate whether S. abortus equi was correlated with an abortion outbreak consisting of 61 abortions. STUDY DESIGN: Investigation of the clinical cases using pathoanatomical, bacteriological, serological and molecular approaches. METHODS: Autopsies on nine aborted foetuses were performed. Tissue specimens from seven aborted foetuses and two mares were cultured and subjected to detection of equine herpesvirus 1 (EHV-1), equine herpesvirus 4 (EHV-4) and equine arteritis virus (EAV) by polymerase chain reaction. The isolates were serotyped according to the Kaufmann-White scheme and analysed by multi-locus sequence typing (MLST). Detection of EHV and EAV antibodies was performed in all pregnant mares. RESULTS: No obvious gross lesions were observed in the foetuses. Nine Gram-negative isolates were obtained from all nine tissue samples and were identified as Salmonella spp. All of the isolates belonged to the B group, the O antigen epitope was 4, 12 and H antigen was not obtained. The isolates of Salmonella were characterised as sequence type (ST) 251. Detection of EHV-1, EHV-4 and EAV was negative. MAIN LIMITATIONS: The mechanism that causes abortion in donkeys needs further study and the lack of histological examination. CONCLUSION: Salmonella abortus equi was isolated as the single agent and was responsible for the abortions. Equine practitioners should be aware of S. abortus equi infection as a cause of abortion in donkeys.


Assuntos
Aborto Animal/microbiologia , Equidae , Complicações Infecciosas na Gravidez/veterinária , Salmonelose Animal/microbiologia , Salmonella/classificação , Aborto Animal/epidemiologia , Animais , China , Feminino , Gravidez , Complicações Infecciosas na Gravidez/epidemiologia , Complicações Infecciosas na Gravidez/microbiologia , Salmonelose Animal/epidemiologia , Salmonelose Animal/patologia
15.
Lab Chip ; 8(5): 822-5, 2008 May.
Artigo em Inglês | MEDLINE | ID: mdl-18432356

RESUMO

The recent proliferation of platforms designed to handle arrays of nano- and picolitre volumes is in response to the need to perform biological assays on discrete entities, such as single cells. However, a critical challenge associated with this trend for in vitro compartmentalization is the need for highly sensitive, yet low-volume detection platforms. In this paper, we coupled confocal fluorescence detection with recirculating microfluidic control to perform single particle DNA assays within five nL chambers. The performance of this low-volume assay was shown to match that of traditional single molecule detection platforms. However, volume requirements per measurement were nearly 3 orders of magnitude less than conventional systems, enabling future integration with lab-on-a-chip systems that require discrete or digitalized sample processing.


Assuntos
DNA/análise , Globinas/análise , Técnicas Analíticas Microfluídicas/métodos , Globinas/genética , Humanos , Técnicas Analíticas Microfluídicas/instrumentação , Microscopia Confocal/instrumentação , Microscopia Confocal/métodos , Microscopia de Fluorescência/instrumentação , Microscopia de Fluorescência/métodos , Microesferas , Fatores de Tempo
16.
Bioinformatics ; 23(2): 198-206, 2007 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-17077095

RESUMO

MOTIVATION: Our purpose is to develop a statistical modeling approach for cancer biomarker discovery and provide new insights into early cancer detection. We propose the concept of dependence network, apply it for identifying cancer biomarkers, and study the difference between the protein or gene samples from cancer and non-cancer subjects based on mass-spectrometry (MS) and microarray data. RESULTS: Three MS and two gene microarray datasets are studied. Clear differences are observed in the dependence networks for cancer and non-cancer samples. Protein/gene features are examined three at one time through an exhaustive search. Dependence networks are constructed by binding triples identified by the eigenvalue pattern of the dependence model, and are further compared to identify cancer biomarkers. Such dependence-network-based biomarkers show much greater consistency under 10-fold cross-validation than the classification-performance-based biomarkers. Furthermore, the biological relevance of the dependence-network-based biomarkers using microarray data is discussed. The proposed scheme is shown promising for cancer diagnosis and prediction. AVAILABILITY: See supplements: http://dsplab.eng.umd.edu/~genomics/dependencenetwork/


Assuntos
Biomarcadores Tumorais/análise , Diagnóstico por Computador/métodos , Espectrometria de Massas/métodos , Proteínas de Neoplasias/análise , Neoplasias/diagnóstico , Neoplasias/metabolismo , Análise de Sequência com Séries de Oligonucleotídeos/métodos , Algoritmos , Simulação por Computador , Perfilação da Expressão Gênica/métodos , Humanos , Modelos Biológicos , Transdução de Sinais
17.
IEEE Trans Signal Process ; 53(9): 3473-3487, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18769527

RESUMO

The positron emission tomography (PET) imaging technique enables the measurement of receptor distribution or neurotransmitter release in the living brain and the changes of the distribution with time and thus allows quantification of binding sites as well as the affinity of a radioligand. However, quantification of receptor binding studies obtained with PET is complicated by tissue heterogeneity in the sampling image elements (i.e., voxels, pixels). This effect is caused by a limited spatial resolution of the PET scanner. Spatial heterogeneity is often essential in understanding the underlying receptor binding process. Tracer kinetic modeling also often requires an intrusive collection of arterial blood samples. In this paper, we propose a likelihood-based framework in the voxel domain for quantitative imaging with or without the blood sampling of the input function. Radioligand kinetic parameters are estimated together with the input function. The parameters are initialized by a subspace-based algorithm and further refined by an iterative likelihood-based estimation procedure. The performance of the proposed scheme is examined by simulations. The results show that the proposed scheme provides reliable estimation of factor time-activity curves (TACs) and the underlying parametric images. A good match is noted between the result of the proposed approach and that of the Logan plot. Real brain PET data are also examined, and good performance is observed in determining the TACs and the underlying factor images.

18.
IEEE Trans Biomed Eng ; 65(3): 489-501, 2018 03.
Artigo em Inglês | MEDLINE | ID: mdl-28463185

RESUMO

In this paper, we introduce TR-BREATH, a time-reversal (TR)-based contact-free breathing monitoring system. It is capable of breathing detection and multiperson breathing rate estimation within a short period of time using off-the-shelf WiFi devices. The proposed system exploits the channel state information (CSI) to capture the miniature variations in the environment caused by breathing. To magnify the CSI variations, TR-BREATH projects CSIs into the TR resonating strength (TRRS) feature space and analyzes the TRRS by the Root-MUSIC and affinity propagation algorithms. Extensive experiment results indoor demonstrate a perfect detection rate of breathing. With only 10 s of measurement, a mean accuracy of can be obtained for single-person breathing rate estimation under the non-line-of-sight (NLOS) scenario. Furthermore, it achieves a mean accuracy of in breathing rate estimation for a dozen people under the line-of-sight scenario and a mean accuracy of in breathing rate estimation of nine people under the NLOS scenario, both with 63 s of measurement. Moreover, TR-BREATH can estimate the number of people with an error around 1. We also demonstrate that TR-BREATH is robust against packet loss and motions. With the prevailing of WiFi, TR-BREATH can be applied for in-home and real-time breathing monitoring.


Assuntos
Taxa Respiratória/fisiologia , Processamento de Sinais Assistido por Computador , Tecnologia sem Fio , Algoritmos , Desenho de Equipamento , Humanos , Internet , Modelos Estatísticos
19.
Neurogastroenterol Motil ; 19(6): 515-21, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17564633

RESUMO

The aim of the present study was to investigate the effect of ethanol on colon motility in rats and to test the possibility that nitric oxide (NO) mediates this effect. Proximal colon longitudinal muscle strips (LM) (8 x 3 mm) cut parallel to the longitudinal muscle fibres of the colon were isolated and mounted in an organ bath. Ethanol (0.57, 0.87 and 1.30 mmol L(-1)) dose-dependently inhibited the motility of LM. Longitudinal muscle strips from female rats were more sensitive to the inhibitory effect of ethanol than that from male rats. L-NAME (N-nitro-L-arginine methyl ester) (100 micromol L(-1)), AG (aminoguanidine) (10 micromol L(-1)), ODQ (1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one) (10 micromol L(-1)) and PTIO (2-Phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide) (200 micromol L(-1)) partly blocked the inhibitory effect of ethanol on LM. Pretreatment with L-NAME, AG, ODQ and PTIO abolished the sex difference of the inhibitory effect of ethanol on LM. Tetrodotoxin (TTX) (10 micromol L(-1)) partly blocked the inhibitory effect but did not influence the sex difference. The relaxation of LM induced by SNP (sodium nitroprusside) (0.1-10 micromol L(-1)) in female rats was greater than that in male rats. In conclusion, ethanol inhibited the colon motility in vitro. This inhibitory effect on LM was mediated by NO through the iNOS - NO - cGMP pathway.


Assuntos
Depressores do Sistema Nervoso Central/farmacologia , Etanol/farmacologia , Motilidade Gastrointestinal/efeitos dos fármacos , Músculo Liso/efeitos dos fármacos , Óxido Nítrico/metabolismo , Animais , Colo/efeitos dos fármacos , Colo/metabolismo , Relação Dose-Resposta a Droga , Inibidores Enzimáticos/farmacologia , Feminino , Motilidade Gastrointestinal/fisiologia , Masculino , Músculo Liso/metabolismo , Doadores de Óxido Nítrico/farmacologia , Nitroprussiato/farmacologia , Técnicas de Cultura de Órgãos , Venenos/farmacologia , Ratos , Fatores Sexuais , Tetrodotoxina/farmacologia
20.
Phys Med Biol ; 52(19): 5831-54, 2007 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-17881803

RESUMO

Tumor motion induced by respiration presents a challenge to the reliable delivery of conformal radiation treatments. Real-time motion compensation represents the technologically most challenging clinical solution but has the potential to overcome the limitations of existing methods. The performance of a real-time couch-based motion compensation system is mainly dependent on two aspects: the ability to infer the internal anatomical position and the performance of the feedback control system. In this paper, we propose two novel methods for the two aspects respectively, and then combine the proposed methods into one system. To accurately estimate the internal tumor position, we present partial-least squares (PLS) regression to predict the position of the diaphragm using skin-based motion surrogates. Four radio-opaque markers were placed on the abdomen of patients who underwent fluoroscopic imaging of the diaphragm. The coordinates of the markers served as input variables and the position of the diaphragm served as the output variable. PLS resulted in lower prediction errors compared with standard multiple linear regression (MLR). The performance of the feedback control system depends on the system dynamics and dead time (delay between the initiation and execution of the control action). While the dynamics of the system can be inverted in a feedback control system, the dead time cannot be inverted. To overcome the dead time of the system, we propose a predictive feedback control system by incorporating forward prediction using least-mean-square (LMS) and recursive least square (RLS) filtering into the couch-based control system. Motion data were obtained using a skin-based marker. The proposed predictive feedback control system was benchmarked against pure feedback control (no forward prediction) and resulted in a significant performance gain. Finally, we combined the PLS inference model and the predictive feedback control to evaluate the overall performance of the feedback control system. Our results show that, with the tumor motion unknown but inferred by skin-based markers through the PLS model, the predictive feedback control system was able to effectively compensate intra-fraction motion.


Assuntos
Artefatos , Modelos Biológicos , Neoplasias/diagnóstico por imagem , Neoplasias/radioterapia , Intensificação de Imagem Radiográfica/métodos , Interpretação de Imagem Radiográfica Assistida por Computador/métodos , Restrição Física/métodos , Robótica/métodos , Algoritmos , Leitos , Simulação por Computador , Sistemas Computacionais , Retroalimentação , Movimento , Radioterapia Assistida por Computador/métodos , Radioterapia Conformacional/métodos , Mecânica Respiratória
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