Development and application of the Ames test using a direct-exposure module: The assessment of mutagenicity of incense and sidestream cigarette smoke.

We had previously developed an improved Ames module to directly determine the mutagenicity of gaseous formaldehyde (HCHO) and toluene without liquid extraction. This study further evaluated the suitability and sensitivity of this module on whole and real polluted air samples. For this, two common brands of stick incense (A and B) and cigarettes (A and B) were harvested, and various types of incense smoke (IS) and sidestream cigarette smoke (SCS) samples were generated by lighting 3, 6, 12, 24, 30, or 36 incense sticks, and by lighting 1, 2, or 3 cigarettes, respectively, in an acrylic box. CO2 , CO, total volatile organic compound (TVOC), PM1.0, and HCHO concentrations in the air samples were determined, and all air samples did not partially fit the requirements of the air quality standards. The smoke samples were then directly exposed to TA100 for 10, 20, 30, or 60 min in our exposure module. Exposure to IS (brand A) for 30 to 60 min and exposure to IS (brand B) for 60 min led to statistically (p < 0.05) weak (below the twofold rule) but dose-dependent mutagenic activities either with or without metabolic activation. Furthermore, a short-term exposure (10-60 min) to SCS (brands A and B) displayed statistically significant (p < 0.05) direct-acting, indirect-acting, time- and dose-dependent mutagenic activities. Furthermore, our data also support that the liver S9 enzyme could enhance the mutagenic activities in most IS and SCS samples. This study confirmed that the modified Ames module can be applied to directly detect the mutagenic activities of real polluted air samples.

Assessment of the mutagenicity of two common indoor air pollutants, formaldehyde and toluene.

Traditionally, direct-reading instruments have been used to directly determine the concentrations of indoor air pollutants that may exceed the regulation limits. However, these instruments cannot directly assess the potential health hazards of these pollutants to humans. In this study, we developed and improved a bacterial reverse mutation assay (Ames test) by using a direct gas exposure module to directly determine the mutagenicity of indoor air quality using five tester bacterial strains (TA98, TA100, TA102, TA1535, and TA1537). Thereafter, the module was used to evaluate the effects of exposure time, different concentrations of HCHO or toluene, and mutagenic activities. We found that TA100 was the most sensitive strain and was reverted by relatively lower concentrations of 0.035 ppm HCHO. Furthermore, 50 ppm of toluene exposures caused a significant increase in the number of revertant colonies of TA100 without S9 activation at the 1.5-8-h exposure time intervals. Our findings provide new evidence that gaseous HCHO exposure could display weak but direct, time-dependent, and dose-dependent mutagenic activities. The weak, direct-acting, indirect-acting, and time-dependent mutagen of 50 ppm toluene was also confirmed. Moreover, our improved Ames module and the exposure conditions provided in this study can be further applied to evaluate the mutagenicity of indoor air quality.

Sequential extraction for heavy metal distribution of bottom ash from fluidized bed co-combusted phosphorus-rich sludge under the agglomeration/defluidization process.

Agglomeration that occurs during municipal sewage sludge (MSS) fluidized bed co-combustion might affect heavy metal distribution and the transformation of bottom ash. A study on the mobility and speciation of heavy metals that accompanies agglomeration behavior and phosphorus addition should be examined during MSS co-combustion. Meanwhile, the aim of this study was to evaluate the total content and speciation of heavy metals during the MSS fluidized bed co-combustion by the chemical sequential extraction procedure (SEP). The risk assessment code (RAC) and individual contamination factor (ICF) are calculated to evaluate the mobility of heavy metals and their environmental risks in agglomerates. Moreover, identification of agglomerates is established by both characterization (scanning electron microscopy/energy-dispersive spectroscopy, X-ray photoelectron spectroscopy) and thermodynamic simulation (HSC chemistry software). The experimental results indicated that P and Na would form the lower melting-point compounds such as NaPO3 and Na2O in the bottom ash, which promoted agglomeration during MSS fluidized bed co-combustion. According to the simulation, Na and P have a stronger affinity than Si and Cr, and this reaction is not only influenced by particle agglomeration, but also by heavy metal distribution during modified MSS co-combustion. Nevertheless, the results of ICFs and RACs obtained from the SEP indicated that for heavy metals trapped in agglomerates, a weaker binding such as physical covering by eutectics might be considered as the dominant reaction compared with chemical binding to form a metal complex.

Prebiotic effects of bovine lactoferrin on specific probiotic bacteria.

Bovine lactoferrin (bLf) is a natural iron-binding protein and it has been suggested to be a prebiotic agent, but this finding remains inconclusive. This study explores the prebiotic potential of bLf in 14 probiotics. Initially, bLf (1-32 mg/mL) treatment showed occasional and slight prebiotic activity in several probiotics only during the late experimental period (48, 78 h) at 37 °C. We subsequently supposed that bLf exerts stronger prebiotic effects when probiotic growth has been temperately retarded. Therefore, we incubated the probiotics at different temperatures, namely 37 °C, 28 °C, room temperature (approximately 22-24 °C), and 22 °C, to retard or inhibit their growth. As expected, bLf showed more favorable prebiotic activity in several probiotics when their growth was partially retarded at room temperature. Furthermore, at 22 °C, the growth of Bifidobacterium breve, Lactobacillus coryniformis, L. delbrueckii, L. acidophilus, B. angulatum, B. catenulatum, and L. paraplantarum were completely blocked. Notably, these probiotics started regrowing in the presence of bLf (1-32 mg/mL) in a significant and dose-dependent manner. Accordingly, bLf significantly increased the growth of Pediococcus pentosaceus, L. rhamnosus, and L. paracasei (BCRC 17483; a locally isolated strain) when their growth was retarded by incubation at 22 °C. In conclusion, bLf showed inconsistent prebiotic activity in the 14 probiotics at 37 °C, but revealed strong prebiotic activity in 10 probiotic strains at 22 °C. Therefore, this study enables determining additional roles of Lf in probiotic strains, which can facilitate developing novel combinational approaches by simultaneously using Lf and specific probiotics.

Simultaneous removal of sulfur dioxide and polycyclic aromatic hydrocarbons from incineration flue gas using activated carbon fibers.

Incineration flue gas contains polycyclic aromatic hydrocarbons (PAHs) and sulfur dioxide (SO2). The effects of SO2 concentration (0, 350, 750, and 1000 ppm), reaction temperature (160, 200, and 280 degrees C), and the type of activated carbon fibers (ACFs) on the removal of SO2 and PAHs by ACFs were examined in this study. A fluidized bed incinerator was used to simulate practical incineration flue gas. It was found that the presence of SO2 in the incineration flue gas could drastically decrease removal of PAHs because of competitive adsorption. The effect of rise in the reaction temperature from 160 to 280 degrees C on removal of PAHs was greater than that on SO2 removal at an SO2 concentration of 750 ppm. Among the three ACFs studied, ACF-B, with the highest microporous volume, highest O content, and the tightest structure, was the best adsorbent for removing SO2 and PAHs when these gases coexisted in the incineration flue gas. Implications: Simultaneous adsorption of sulfur dioxide (SO2) and polycyclic aromatic hydrocarbons (PAHs) emitted from incineration flue gas onto activated carbon fibers (ACFs) meant to devise a new technique showed that the presence of SO2 in the incineration flue gas leads to a drastic decrease in removal of PAHs because of competitive adsorption. Reaction temperature had a greater influence on PAHs removal than on SO2 removal. ACF-B, with the highest microporous volume, highest O content, and tightest structure among the three studied ACFs, was found to be the best adsorbent for removing SO2 and PAHs.

Effects of activated carbon fibre-supported metal oxide characteristics on toluene removal.

Few studies have investigated the use of activated carbon fibres (ACFs) impregnated with metal oxides for the catalytic oxidation of volatile organic compounds (VOCs). Thus, the effects of the ACF-supported metal oxides on toluene removal are determined in this study. Three catalysts, namely, Ce, Mn, and Cu, two pretreatment solutions NaOH and H2O2, and three reaction temperatures of 250 degrees C, 300 degrees C, and 350 degrees C, were employed to determine toluene removal. The composition and morphology of the catalysts were analysed using Brunauer-Emmett-Teller (BET), transmission electron microscope (TEM), inductively coupled plasma (ICP), X-ray photoelectron spectroscopy (XPS), Fourier-transform infrared spectrometer (FTIR), and thermo-gravimetric analyser (TGA) to study the effects of the catalyst's characteristics on toluene removal. The results demonstrated that the metal catalysts supported on the ACFs could significantly increase toluene removal. The Mn/ACFs and Cu/ACFs were observed to be most active in toluene removal at a reaction temperature of 250 degrees C with 10% oxygen content. Moreover, the data also indicated that toluene removal was slightly improved after pretreating the ACFs with NaOH and H2O2. The results suggested that surface-metal loading and the surface characteristics of the ACFs were the determinant parameters for toluene removal. Furthermore, the removal of toluene over Mn/ACFs-H202 decreased when the reaction temperature considered was > 300 degrees C.

Evaluation of a Standard Dietary Regimen Combined with Heat-Inactivated Lactobacillus gasseri HM1, Lactoferrin-Producing HM1, and Their Sonication-Inactivated Variants in the Management of Metabolic Disorders in an Obesity Mouse Model.

This study investigated the impact of incorporating various inactivated probiotic formulations, with or without recombinant lactoferrin (LF) expression, into a standard chow diet on metabolic-related disorders in obese mice. After inducing obesity through a 13-week high-fat diet followed by a standard chow diet, mice received daily oral administrations of different probiotics for 6 weeks using the oral gavage approach. These probiotic formulations consisted of a placebo (MRS), heat-inactivated Lactobacillus gasseri HM1 (HK-HM1), heat-killed LF-expression HM1 (HK-HM1/LF), sonication-killed HM1 (SK-HM1), and sonication-killed LF-expression HM1 (SK-HM1/LF). The study successfully induced obesity, resulting in worsened glucose tolerance and insulin sensitivity. Interestingly, the regular diet alone improved glucose tolerance, and the addition of inactivated probiotics further enhanced this effect, with SK-HM1/LF demonstrating the most noticeable improvement. However, while regular dietary intervention alone improved insulin sensitivity, probiotic supplementation did not provide additional benefits in this aspect. Inflammation in perirenal and epididymal fat tissues was partially alleviated by the regular diet and further improved by probiotics, particularly by SK-HM1, which showed the most significant reduction. Additionally, HK-HM1 and HK-HM1/LF supplements could contribute to the improvement of serum total triglycerides or total cholesterol, respectively. Overall, incorporating inactivated probiotics into a regular diet may enhance metabolic indices, and recombinant LF may offer potential benefits for improving glucose tolerance.

Assessment of inhalation exposure to microplastic particles when disposable masks are repeatedly used.

Wearing masks to prevent infectious diseases, especially during the COVID-19 pandemic, is common. However, concerns arise about inhalation exposure to microplastics (MPs) when disposable masks are improperly reused. In this study, we assessed whether disposable masks release inhalable MPs when reused in simulated wearing conditions. All experiments were conducted using a controlled test chamber setup with a constant inspiratory flow. Commercially available medical masks with a three-layer material, composition comprising polypropylene (PP in the outer and middle layers) and polyethylene (PE in the inner layer), were used as the test material. Brand-new masks with and without hand rubbing, as well as reused medical masks, were tested. Physical properties (number, size, and shape) and chemical composition (polymers) were identified using various analytical techniques such as fluorescence staining, fluorescence microscopy, and micro-Fourier Transform Infrared Spectroscopy (µFTIR). Scanning Electron Microscopy (SEM) was used to scrutinize the surface structure of reused masks across different layers, elucidating the mechanism behind the MP generation. The findings revealed that brand-new masks subjected to hand rubbing exhibited a higher cumulative count of MPs, averaging approximately 1.5 times more than those without hand rubbing. Fragments remained the predominant shape across all selected size classes among the released MPs from reused masks, primarily through a physical abrasion mechanism, accounting for >90 % of the total MPs. The numbers of PE particles were higher than PP particles, indicating that the inner layer of the mask contributed more inhalable MPs than the middle and outer layers combined. The released MPs from reused masks reached their peak after 8 h of wearing. This implies that regularly replacing masks serves as a preventive measure and mitigates associated health risks of inhalation exposure to MPs.

Featured Prebiotic Agent: The Roles and Mechanisms of Direct and Indirect Prebiotic Activities of Lactoferrin and Its Application in Disease Control.

Lactoferrin (LF) is a glycoprotein found in mammalian milk, and lactoferricin is a peptide derived from LF hydrolysate. Both LF and lactoferricin (LFcin) have diverse functions that could benefit mammals. Bovine LF (BLF) and BLFcin exhibit a wide range of antimicrobial activities, but most probiotic strains are relatively resistant to their antibacterial effects. BLF and BLF hydrolysate can promote the growth of specific probiotics depending on the culture conditions, the dose of BLF or BLF-related peptides, and the probiotic strains used. BLF supplementation has been shown to modulate several central molecular pathways or genes in Lacticaseibacillus rhamnosus GG under cold conditions, which may explain the prebiotic roles of BLF. LF alone or in combination with selected probiotics can help control bacterial infections or metabolic disorders, both in animal studies and in human clinical trials. Various LF-expressing probiotics, including those expressing BLF, human LF, or porcine LF, have been developed to facilitate the combination of LFs with specific probiotics. Supplementation with LF-expressing probiotics has positive effects in animal studies. Interestingly, inactivated LF-expressing probiotics significantly improved diet-induced nonalcoholic fatty liver disease (NAFLD) in a mouse model. This review highlights the accumulated evidence supporting the use of LF in combination with selected LF-resistant probiotics or LF-expressing probiotics in the field.

Therapeutic effects of orally administration of viable and inactivated probiotic strains against murine urinary tract infection.

Urinary tract infections (UTIs) are highly prevalent bacterial infections that pose significant health risks. Specific probiotic strains have been recommended for UTI control and management of antibiotic resistance. Otherwise, para-probiotics, defined as inactivated probiotic cells, offer potential advantages by minimizing risks associated with live microorganisms. However, the effectiveness of heat-killed probiotic strains against UTIs remains uncertain. Additionally, lactoferrin (LF), an iron-binding glycoprotein, exhibits immunomodulatory, antimicrobial, and anti-inflammatory properties. Recently, we had developed recombinant LF-expression probiotics, which can display considerate antibacterial activities against select food-borne pathogens in vitro. Thus, the present study aimed to evaluate the antibacterial activities of heat-killed natural and recombinant LF-expressing probiotics against UTIs in vitro and in vivo. Firstly, using in vitro assays, we assessed the antibacterial activity of heat-killed natural and recombinant LF-expressing probiotics against uropathogenic Escherichia coli and Klebsiella pneumoniae. Among the tested probiotics, 10 heat-killed LF-expressing strains displayed superior antibacterial efficacy compared to 12 natural probiotics. Based on their potent in vitro activity, selected probiotics were formulated into three probiotic mixtures: viable probiotic mixture (LAB), heat-killed probiotic mixture (HK-LAB), and heat-killed LF-expressing probiotic mixture (HK-LAB/LF). To further evaluate the therapeutic potential of these probiotic mixtures in vivo, we established a murine model of UTIs by intraurethral administration of E. coli to 40 female C57BL/6JNarl mice on day 0. Subsequently, mice received oral gavage of placebo, LAB, HK-LAB, or HK-LAB/LF for 21 consecutive days (n = 8 per group). An additional control group (n = 8) received ampicillin treatment for 7 days. To assess protective effects against re-infection or UTI relapse, all mice were challenged with E. coli on day 22 and E. coli plus K. pneumoniae on day 25. Results from the murine UTI model demonstrated that placebo administration did not reduce bacteriuria throughout the experiment. Conversely, supplementation with ampicillin, HK-LAB/LF, HK-LAB, or LAB significantly (p < 0.05) reduced daily bacteriuria by 103 to 104-fold on days 1, 3, 5, and 14, respectively. Furthermore, all four therapeutic treatments improved the bacteriological cure rate (BCR) with varying levels of efficacy. For the 7-day treatment course, the BCR was 25% (placebo), 62.5% (ampicillin), 37.5% (LAB), 37.5% (HK-LAB), and 62.5% (HK-LAB/LF). For the 21-day treatment course, the BCR was 25% (placebo), 75% (ampicillin), 37.5% (LAB), 37.5% (HK-LAB), and 75% (HK-LAB/LF). Notably, HK-LAB and HK-LAB/LF demonstrated superior therapeutic efficacy compared to viable LAB in treating UTIs. Overall, regarding BCR, the three probiotic mixtures can provide benefits against UTI in mice, but ampicillin therapy remains the most efficient among the four treatments. Furthermore, there was no significant difference between pre- and post-challenge courses for the two instances of re-challenging uropathogens in all mice groups, as bacteriuria levels remained below 103 CFU/mL, implying that adaptive responses of mice may help reduce the risk of recurrent UTIs. In conclusion, our results provide new evidence that oral administration of heat-killed probiotic mixtures can confer significant therapeutic efficacy against UTIs in a murine model.

Characteristics of PM and PAHs emitted from a coal-fired boiler and the efficiencies of its air pollution control devices.

Sampling and analysis of filterable particulate matter (FPM), FPM2.5, condensable particulate matter (CPM), polycyclic aromatic hydrocarbons (PAHs), sulfur oxides (SOx), and nitrogen oxides (NOx) emitted from a coal-fired boiler equipped with selective catalytic reduction (SCR)+ electrostatic precipitator (ESP) + wet flue gas desulfurization (WFGD) + wet electrostatic precipitator (WESP) as air pollution control devices (APCDs) are conducted. The results show that NOx concentration emitted from the coal-fired boiler is 56 ± 2.17 ppm (with the NOx removal efficiency of 47.2%), which does not meet the best available control technology (BACT) emission standard (≤ 30 ppm). On the other hand, the WFGD adopted has a good removal efficiency for SOx and HCl. Both SOx and HCl emission concentrations are < 1 ppm, and removal efficiencies are > 99%. The FPM and FPM2.5 emitted from the coal-fired boiler are 0.9 ± 0.06 mg/Nm3 and < 0.09 ± 0.006 mg/Nm3, respectively. The overall removal efficiency of FPM achieved with ESP+WFGD+WESP+MGGH is 99.98%. However, high concentration of CPM (37.4 ± 6.3 mg/Nm3) is measured, which is significantly higher than FPM and FPM2.5. The concentrations of 27 PAHs at the WESP inlet and stack are measured as 667 ng/Nm3 and 547 ng/Nm3, respectively while the removal efficiencies of gas- and solid-phase PAHs are 9% and 58%, respectively. The results show that APCDs adopted are not effective in removing PAHs (only 18%), and gas-phase PAHs contribute the most in the total PAH emission. In addition, the benzo(a)pyrene equivalent (BaPeq) concentration emitted from the stack is 28.8 ng-BaPeq/Nm3, and most of it is contributed by 4-6 ring PAHs with high toxic equivalent factors (TEFs). Furthermore, the emission factors of air pollutant emitted from coal-fired boilers equipped with different combinations of APCDs are compiled and compared. The results show that except for CPM and NOx, the emission factors of air pollutant calculated for this coal-fired boiler are lower if compared with other studies.Implications: Primary particles discharged from coal-fired processes include filterable particulate matter (FPM) and condensable particulate matter (CPM). PM2.5 emissions would be greatly underestimated if CPM is ignored. Polycyclic aromatic hydrocarbons (PAHs) are semi-volatile organic compounds (SVOCs) formed by two or more fused benzene rings. PAHs have attracted much public attention because of toxicity and carcinogenicity. This study selects one coal-fired boiler with the best available control technology (BACT) to simultaneously measure the concentrations of PM, PAHs, and gaseous pollutants at the inlet and outlet of air pollution control devices (APCDs) to understand the efficacy of APCDs adopted and pollutant emission intensity.

Oral Administration of Recombinant Lactoferrin-Expressing Probiotics Ameliorates Diet-Induced Lipid Accumulation and Inflammation in Non-Alcoholic Fatty Liver Disease in Mice.

We have recently developed probiotics that can express bovine, human, or porcine lactoferrin (LF), and the present study evaluated the effect of these probiotics in improving non-alcoholic fatty liver disease (NAFLD). Three kinds of probiotic supplements, including lactic acid bacteria (LAB), LAB/LF, and inactivated LAB/LF, were prepared. The LAB supplement was prepared from 10 viable LAB without recombinant LF-expression, the LAB/LF supplement was prepared from 10 viable probiotics expressing LF, and the inactivated LAB/LF supplement was prepared from 10 inactivated probiotics expressing LF. A model of obese/NAFLD mice induced by a high-fat diet was established, and the mice were randomly divided into four groups and fed with a placebo, LAB, LAB/LF, or inactivated LAB daily for four weeks via oral gavage. The body weight, food intake, organ weight, biochemistry, and hepatic histopathological alterations and severity scoring were measured. The results revealed that the obese mice fed with any one of the three probiotic mixtures prepared from recombinant probiotics for four weeks exhibited considerably improved hepatic steatosis. These findings confirmed the assumption that specific probiotic strains or LF supplements could help to control NAFLD, as suggested in previous reports. Our data also suggest that the probiotics and LFs in probiotic mixtures contribute differently to improving the efficacy against NAFLD, and the expressed LF content in probiotics may help to boost their efficacy in comparison with the original probiotic mixtures. Moreover, when these LF-expressing probiotics were further inactivated by sonication, they displayed better efficacies than the viable probiotics against NAFLD. This study has provided intriguing data supporting the potential of recombinant probiotics in improving hepatic steatosis.

Transcriptome analysis of Lactobacillus rhamnosus GG strain treated with prebiotic - bovine lactoferrin under a cold environment.

Lactoferrin (LF) is secreted by mammals and displays extensive biological effects. We previously reported that bovine LF (BLF) can boost the cold tolerance of a well-applied probiotic strain, Lactobacillus rhamnosus GG (LGG), to grow robustly under a cold environment, but the molecular mechanism is not clear. Here, RNA-seq analysis was conducted to ascertain molecular pathways underlying cold tolerance exerted by BLF. LGG was cultured in a cold environment (22 °C) in the presence or absence of BLF. Transcriptome analysis indicated that BLF significantly elicited 1.2-3.2 fold (log2 Fold change) higher expression levels of genes related to stress, defense, cell division, and transporter in LGG, including the genes CspA, LytR, XRE, MerR, and GpsB. The KEGG pathway and GO analyses confirmed that BLF can modulate a few central pathways to boost the growth of LGG. BLF also reduced metabolic pathways involved in purine, amino acid, pyrimidine, one-carbon metabolism, and secondary metabolites in LGG. We speculate that the reduction of the above pathways may play key roles to reduce energy requirement and maintain carbon metabolism balance in LGG for surviving and growing in a cold state, and BLF can be an excellent prebiotic to LGG cultured in this cold condition (22 °C). Overall, this study uncovers the molecular effects of BLF on LGG.

Predicting carbonaceous aerosols and identifying their source contribution with advanced approaches.

Organic carbon (OC) and elemental carbon (EC) play important roles in various atmospheric processes and health effects. Predicting carbonaceous aerosols and identifying source contributions are important steps for further epidemiological study and formulating effective emission control policies. However, we are not aware of any study that examined predictions of OC and EC, and this work is also the first study that attempted to use machine learning and hyperparameter optimization method to predict concentrations of specific aerosol contaminants. This paper describes an investigation of the characteristics and sources of OC and EC in fine particulate matter (PM2.5) from 2005 to 2010 in the City of Taipei. Respective hourly average concentrations of OC and EC were 5.2 µg/m3 and 1.6 µg/m3. We observed obvious seasonal variation in OC but not in EC. Hourly and daily OC and EC concentrations were predicted using generalized additive model and grey wolf optimized multilayer perceptron model, which could explain up to about 80% of the total variation. Subsequent clustering suggests that traffic emission was the major contribution to OC, accounting for about 80% in the spring, 65% in the summer, and 90% in the fall and winter. In the Taipei area, local emissions were the dominant sources of OC and EC in all seasons, and long-range transport had a significant contribution to OC and in PM2.5 in spring.

A Single Plasmid of Nisin-Controlled Bovine and Human Lactoferrin Expressing Elevated Antibacterial Activity of Lactoferrin-Resistant Probiotic Strains.

Lactoferrin (LF) is a multifunctional protein found in mammals, and it shows broad-spectrum antimicrobial activity. To improve the functional properties of specific probiotics in order to provide both the beneficial characteristics of lactic acid bacteria and the biological activity of LF, cDNAs of bovine LF (BLF), human LF (HLF), or porcine LF (PLF) were cloned into a nisin-inducible plasmid. These were then transformed into the selected eight probiotics, which are LF-resistant hosts. Expression of recombinant LFs (rLFs) was analyzed via SDS-PAGE and Western blot analysis. Although the selected host strains may not contain the nisRK genes (NisK, the sensor kinase; NisR, the regulator protein), the components of autoregulation, a low level of LFs expression can be successfully induced by using nisin within bacterial cells in a time-dependent manner in three engineered clones, including Lactobacillus delbrueckii/HLF, L. delbrueckii/BLF, and L. gasseri/BLF. Lactobacillus delbrueckii and Lactobacillus gasseri originate from yogurt and human milk, respectively, and both strains are functional probiotic strains. Therefore, we further compared the antibacterial activities of disrupted recombinant probiotic clones, conventional strains (host control), and vector control ones by using agar diffusion and broth inhibition analysis, and the expression of rLFs in the above three clones considerately improved their antibacterial efficacies against four important food-borne pathogens, namely, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, and Salmonellaenterica. In conclusion, this study provides a simple strategy for the production of functional LFs (BLF and HLF) in both functional and LF-resistant hosts for applications in the field.

Adsorption of SO2 and NO from incineration flue gas onto activated carbon fibers.

Activated carbon fibers (ACFs) were used to remove SO2 and NO from incineration flue gas. Three types of ACFs in their origin state and after pretreatment with HNO3, NaOH, and KOH were investigated. The removal efficiencies of SO2 and NO were determined experimentally at defined SO2 and NO concentrations and at temperatures of 150, 200 and 260 degrees C. Experimental results indicated that the removal efficiencies of SO2 and NO using the original ACFs were < 56% and < 27%, respectively. All ACFs modified with HNO3, NaOH, and KOH solution could increase the removal efficiencies of SO(2) and NO. The mesopore volumes and functional groups of ACFs are important in determining the removal of SO2 and NO. When the mesopore volumes of the ACFs are insufficient for removing SO2 and NO, the functional groups on the ACFs are not important in determining the removal of SO2 and NO. On the contrary, the effects of the functional groups on the removal of SO2 and NO are more important than the mesopore volumes as the amount of mesopores on the ACFs is sufficient to remove SO2 and NO. Moreover, the removal efficiencies of SO2 and NO were greatest at 200 degrees C. When the inlet concentration of SO2 increased to 600 ppm, the removal efficiency of SO2 increased slightly and the removal efficiency of NO decreased.

Control of heavy metals during incineration using activated carbon fibers.

Activated carbon fibers (ACFs) were applied to control heavy metals in incineration flue gas. Three heavy metal species (Cr, Cd and Pb), three ACFs, various adsorption temperatures (150, 250 and 300 degrees C) and weights of ACFs were experimentally determined. The results indicated that the effects of the type of ACF and the weight of the ACFs on the solid-state Cr removal were insignificant. The extent of solid-state Cd and Pb removal was related to the knitting structure of ACFs and the physical characteristic of the metals. The removal efficiencies of the solid-state and gaseous metals at various reaction temperature followed the order 250>150>300 degrees C and 300>250>150 degrees C, respectively.

Emission characteristics of coal combustion in different O2/N2, O2/CO2 and O2/RFG atmosphere.

This study investigates the emission characteristics of CO(2), SO(2) and NOx in the flue gas of coal combustion by varying the compositions and concentrations of feed gas (O(2)/CO(2)/N(2)) and the ratios of recycled flue gas. The differences between O(2)/recycled flue gas (O(2)/RFG) combustion and general air combustion are also discussed. Experimental results indicate that the maximum concentration of CO(2) in O(2)/CO(2) combustion system is 95% as the feed gas is 30% O(2)/70% CO(2). The average concentration of CO(2) in the flue gas of O(2)/CO(2) coal combustion system is higher than 90% and much higher than that of O(2)/N(2) coal combustion system. This high concentration of CO(2) is beneficial for the separation of CO(2) from the flue gas by adsorption or absorption technologies. The maximum concentration of CO(2) in O(2)/N(2) combustion system is only 34% at the feed gas 50% O(2)/50% N(2), the concentration of CO(2) is increased with the concentration of O(2) in feed gas. By O(2)/CO(2) combustion technology, higher concentration of SO(2) is produced as the feed gas is 30% O(2)/70% CO(2) or 40% O(2)/60% CO(2), while higher concentration of NOx is produced as the feed gas is 20% O(2)/80% CO(2) or 50% O(2)/50% CO(2). The mass flow rates of CO(2), SO(2) and NOx in the flue gas are all increased with the ratio of recycled flue gas except for the feed gas 20% O(2)/80% CO(2). The enhanced mass flow rates of air pollutants in such O(2)/RFG combustion system are also beneficial for improving the control efficiencies of air pollution control devices. By O(2)/N(2) combustion technology, higher concentrations of SO(2) and NOx are produced as the feed gas is 21% O(2)/79% N(2). The results also indicate that the formation of NOx in general air combustion system is higher than that in O(2)/RFG or O(2)/CO(2) combustion system.

Assessment of Cellular Mutagenicity of Americano Coffees from Popular Coffee Chains.

Coffee is a popular beverage worldwide, but coffee beans can be contaminated with carcinogens. The Ames Salmonella mutagenicity test is often used for analysis of carcinogens for mutagenicity. However, previous studies have provided controversial data about the direct mutagenicity of coffee beans based on Ames test results. This study was conducted to determine the mutagenicity of popular Americano coffee based on results from the Ames test. Coffee samples without additives that were served by five international coffee chain restaurants were subjected to the analysis using Salmonella Typhimurium tester strains TA98, TA100, and TA1535. The levels of bacterial revertants in samples from coffee chains were lower than the twofold criterion of the control sets, and no significant dose-response effect was observed with or without rat liver enzyme activation. These data indicate that Americano coffees from the selected coffee chains possessed no direct mutagenic activity with or without enzyme activation. These findings suggest a low mutagenic risk from Americano coffees served by the selected coffee chains and support the use of other methods to confirm the nonmutagenicity of coffee products. These results are consistent with most recent epidemiological reports.

Cellular Mutagenicity and Heavy Metal Concentrations of Leachates Extracted from the Fly and Bottom Ash Derived from Municipal Solid Waste Incineration.

Two incinerators in Taiwan have recently attempted to reuse the fly and bottom ash that they produce, but the mutagenicity of these types of ash has not yet been assessed. Therefore, we evaluated the mutagenicity of the ash with the Ames mutagenicity assay using the TA98, TA100, and TA1535 bacterial strains. We obtained three leachates from three leachants of varying pH values using the toxicity characteristic leaching procedure test recommended by the Taiwan Environmental Protection Agency (Taiwan EPA). We then performed the Ames assay on the harvested leachates. To evaluate the possible relationship between the presence of heavy metals and mutagenicity, the concentrations of five heavy metals (Cd, Cr, Cu, Pb, and Zn) in the leachates were also determined. The concentrations of Cd and Cr in the most acidic leachate from the precipitator fly ash and the Cd concentration in the most acidic leachate from the boiler fly ash exceeded the recommended limits. Notably, none of the nine leachates extracted from the boiler, precipitator, or bottom ashes displayed mutagenic activity. This data partially affirms the safety of the fly and bottom ash produced by certain incinerators. Therefore, the biotoxicity of leachates from recycled ash should be routinely monitored before reusing the ash.