[Changes in neuromediator regulation of the small intestine in experimental alcoholic intoxication].

The effect of ethanol on the bioamin containing structures of the jejunum and ileum walls at different periods of chronic alcoholic intoxication was investigated. The quantitative description of the catecholamine and serotonin content in the enterocytes of epithelial lining villi, submucosa mast cells, crypt enterocytes and muscle membrane is given for the first time by means of luminescent microscopy and cyto spectrofluorimetry. It was established that in the initial period of chronic alcohol intoxication for 60 days in rats increased serotonin effect on intestinal functional structures was observed. On longer terms there is a disturbance of relationships of biogenic amines and the regulating role of catecholamines starts to prevail.

[Alcohol-induced changes of biogenic amine-containing structures of the large intestine].

Using the methods of luminescent microscopy and cytospectrofluorometry, biogenic amine-containing structures were studied in colon of rats subjected to alcohol intoxication of 2, 4 and 6 months' duration. It was demonstrated that 2- and 4-month-long alcohol intoxication of animals resulted in biogenic amine content increase in the mucosal surface epithelium, submucosal mast cells and adrenergic nerve fibers within the connective tissue bands in the muscular tunic. At the same time, 6 month-long intoxication was accompanied by a stabilization of bioamine content at the elevated level.

[Reaction of monoamine-containing structures of rat spleen to acupuncture].

Using the method of luminescent microscopy, the catecholamine (CA) and serotonin (S) content was examined in spleen structures, and the interrelations between them were traced in rats before and 15 minutes, 1, 2 and 4 hours after the acupuncture in LI 4 and GV 14 points. It was found that after acupuncture, neurotransmitter content in splenic red and white pulp was changed already in 15 minutes. The changes of CA and S content in granular luminescent cells (GLC) of the germinal centers of spleen lymphoid nodules had a wavy character. At the same time, the correlations between monoamine content in the central arteriole wall and in germinal center GLCs, as well as that one between GLCs and germinal center lymphocytes, became strong positive. The data obtained suggest the presence of an immune-stimulating component in acupuncture, which became apparent during the first hour and persisted for 4 hours after a single acupuncture in GV 14 and LI 4 points.

[Characteristic of bioamine-containing dental pulp structures under normal conditions and in pathology (a luminescence histochemical study)].

Neurotransmitter biogenic amines (BA) are known to be important tissue differentiation inducers and promoters of mutual influence of the epithelial and mesenchymal structures during the process of tooth development in the antenatal period. The aim of this investigation was the study of BA-containing dental pulp structures in the adult humans under normal conditions, and in patients with dental caries and its complications in combination with periodontitis. Using luminescent-histochemical methods, the contents of neurotransmitters (cateholamines, serotonin, histamine) was determined in the dental pulp under normal conditions and in pathology. Qualitative and quantitative changes of BA-containing dental pulp structures are described in patients with dental caries and its complications in combination with periodontitis. It was demonstrated that mast and granular luminescent cells, together with the nerve fibers, were involved in the neurotransmitter control of homeostasis in the dental pulp, both under normal conditions and in pathology, by providing the dental pulp structures with BA. The changes in the content of the neurotransmitter BA were noted already at the stage of initial dental hard tissue lesions and were found to precede the development of an acute pulpitis in combination with intermediate degree periodontitis.

[Dynamics in the content of neuromediators in amine-containing structures during development of tooth hard tissues]. / Dinamika soderzhaniia neiromediatorov v aminosoderzhashchikh strukturakh v period razvitiia tverdykh tkanei zuba.

Using luminescent-histochemical methods the contents of neurotransmitters (catecholamines, serotonin, histamine) was determined during the development of dental hard tissues in man. In the dental papilla the luminescent blood vessels were initially demonstrated, while single nerve fibers branching around the odontoblasts appeared later. It was found that enamel organ differentiation at the early stages took place without the participation of granular luminescent cells and mast cells. These cells appeared starting at week 22 of fetal development. Their appearance coincided with the process of enameloblast and odontoblast differentiation. The contents of neurotransmitters in the dental structures studied was found to increase with the fetal age.

[Distribution of bioactive substances in a developing tooth during late stages of embryogenesis]. / Raspredelenie biologicheski aktivnykh veshchestv v razviviushchemsia zube na pozdnikh étapakh émbriogeneza.

The location of bioactive substances in developing teeth and their effects on the tooth have been studied. Stage-by-stage distribution of bioactive substances and appearance of amino-producing cells have been shown.

[Inductive effect of bioactive substances on dental cell differentiation]. / Induktivnoe vliianie biologicheski aktivnykh veshchestv na protsessy differentsirovki kletok zuba.

Qualitative and quantitative characteristics of neuromediator distribution in developing dental tissues are presented. Mechanisms of involvement of bioactive substances present in granular fluorescent cells and mast cells of the dental pulp in the regulation of tooth development are discussed. Relationship between the content of bioactive substances in the enamel organ and mesenchymal structures of a developing tooth and in bio-amine containing cells and nerve fibers is revealed, which can underlie one of the important mechanisms of their mutual inductive effects during the ontogenesis.

[Luminescence-histochemical analysis of the histamine-containing cells in the lobule of the thymus]. / Liuminestsentno-gistokhimicheskii analiz gistaminsoderzhashchikh kletok timusnoi dol'ki.

By means of the luminescent-histochemical method of S. A. M. Cross, S. W.-B. Ewen and E. W. D. Rost [9] in the premedullary zone of the thymus lobule cortex histamine-containing cells have been found; they have different form, size, luminescent colour. The histamine-containing cells differently react to introduction of biologically active substances: histamine, noradrenaline, acetylcholine. Their amount depends on sex and age of the animals.