Molecular mechanism of resveratrol promoting differentiation of preosteoblastic MC3T3-E1 cells based on network pharmacology and experimental validation.

The purpose of this study was to investigate the mechanism by which resveratrol (Res) inhibits apoptosis and promotes proliferation and differentiation of pre-osteoblastic MC3T3-E1 cells, laying the groundwork for the treatment of osteoporosis (OP). The TCMSP database was used to find the gene targets for Res. The GeneCards database acquire the gene targets for OP. After discovering the potential target genes, GO, KEGG, and Reactome enrichment analysis were conducted. Verifying the major proteins involved in apoptosis can bind to Res using molecular docking. CCK8 measured the proliferative activity of mouse pre-osteoblasts in every group following Res intervention. Alkaline phosphatase staining (ALP) and alizarin red staining to measure the ability of osteogenic differentiation. RT-qPCR to determine the expression levels of Runx2 and OPG genes for osteogenic differentiation ability of cells. Western blot to measure the degree of apoptosis-related protein activity in each group following Res intervention. The biological processes investigated for GO of Res therapeutic OP involved in cytokine-mediated signaling pathway, negative regulation of apoptotic process, Aging, extrinsic apoptotic signaling pathway in absence of ligand, according to potential therapeutic target enrichment study. Apoptosis, FoxO signaling pathway, and TNF signaling pathway are the primary KEGG signaling pathways. Recactome pathways are primarily engaged in Programmed Cell Death, Apoptosis, Intrinsic Apoptotic Pathway, and Caspase activation via extrinsic apoptotic signaling pathways. This research established a new approach for Res treatment of OP by demonstrating how Res controls the apoptosis-related proteins TNF, IL6, and CASP3 to suppress osteoblast death and increase osteoclastogenesis.

Stand spatial structure and microbial diversity are key drivers of soil multifunctionality during secondary succession in degraded karst forests.

Studying the relationship between biodiversity and ecosystem multifunctionality (the ability of ecosystems to provide multiple ecosystem functions) (BEMF) is a current hotspot in ecology research. Previous studies on BEMF emphasized the role of plant and microbial diversity but rarely mention stand spatial structure. To investigate the effect of stand spatial structure on BEMF, this study established 30 forest dynamic plots in three natural restoration stages (shrubbery, secondary growth forest, and old-growth forest) in Maolan National Nature Reserve, Guizhou province, China. A positive response in soil multifunctionality (SMF), plant species diversity, stand spatial structure, and fungal ß diversity (p < 0.05) followed natural restoration. However, bacterial ß diversity showed a negative response (p < 0.05), while microbial α diversity remained unchanged (p > 0.05). These results based on a structural equation model showed that plant species diversity had no direct or indirect effect on SMF, soil microbial diversity was the only direct driver of SMF, and stand spatial structure indirectly affected SMF through soil microbial diversity. The random forest model showed that soil microbial ß diversity and the Shannon-Wiener index of the diameter at breast height for woody plant species were the optimal variables to characterize SMF and soil microbial diversity, respectively. These results suggested that natural restoration promoted SMF, and microbial diversity had a direct positive effect on SMF. In the meantime, stand spatial structure had a significant indirect effect on SMF, while plant species diversity did not. Future work on degraded karst forest restoration should direct more attention to the role of the stand spatial structure and emphasize the importance of biodiversity.

Association between Klebsiella pneumoniae and ankylosing spondylitis: A systematic review and meta-analysis.

AIM: The aim of this study is to evaluate the association between Klebsiella pneumoniae infection and ankylosing spondylitis (AS). METHOD: Five electronic databases, PubMed, Embase, Medline, Web of Science, and Scopes, were searched until September 29, 2021. Cohort and case-control studies that assessed the association between K. pneumoniae infection and AS were included. Pooled odds ratio (OR) was selected to show the effect size. Subgroup analysis (active or inactive AS) and 2 forms of sensitivity analysis were conducted. All statistical analyses were conducted by using STATA 12.0. RESULTS: There were 25 case-control studies finally included, including 8 studies concerning presence of K. pneumoniae in feces, and 17 studies concerning serum antibody (immunoglobulin [Ig]G, IgM, IgA) against K. pneumoniae. The results suggested that when compared with healthy people, presence of K. pneumoniae in feces was associated with AS (OR: 5.65; 95% CI: 1.68-19.00). Similarly, when compared with healthy people, higher positive rates of IgA (OR: 6.28; 95% CI: 3.32-11.91) and IgG (OR: 5.22; 95% CI: 1.36-19.99) were observed. Subgroup analyses suggested that association between K. pneumoniae and AS appears stronger in active AS. CONCLUSION: When compared with healthy people, a significantly higher positive rate of K. pneumoniae in feces, serum IgA and IgG were observed in patients with AS, suggesting that K. pneumoniae probably plays a crucial role in the occurrence of AS. The findings in this study need further prospective investigations for confirmation.