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1.
J Hand Surg Am ; 46(1): 69.e1-69.e7, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33012615

RESUMO

PURPOSE: The goal of this study was to determine whether the type of anesthesia (monitored anesthesia care [MAC] vs wide-awake local anesthesia no tourniquet technique [WALANT]) or the surgical technique (mini-open vs endoscopic) would affect patient satisfaction with postoperative pain control, postoperative pain, or opioid use after carpal tunnel release (CTR). The hypothesis was that endoscopic and open CTR surgery would have the same patient satisfaction with postoperative pain control, postoperative pain, and opioid use, but WALANT surgery would have higher patient satisfaction with postoperative pain control, postoperative pain, and opioid use than MAC. METHODS: This prospective study examined all patients undergoing carpal tunnel surgery by 4 hand surgeons at our institution. Two surgeons perform primarily 1-incision endoscopic CTR and the other 2 perform mini-open CTR. Two surgeons perform all procedures under WALANT; the other 2 employ MAC with a local anesthetic. Postsurgical questionnaires were completed at the 2-week postoperative visit. Patients reported remaining pills, average pain, highest pain, lowest pain, and overall satisfaction with postoperative pain control. RESULTS: A total of 93 patients underwent CTR by the 4 participating hand surgeons. Of these, 43 underwent open CTR and 50 underwent endoscopic CTR. Sixty-two were performed under MAC and 31 with WALANT. With regard to anesthesia type, overall there was 5.5 mean morphine equivalents (MME) less prescribed and an average of 3.6 MME more remaining on the first postoperative visit with WALANT compared with MAC. Patient satisfaction with postoperative pain control was an average score of 7.9 for MAC and 7.4 for WALANT. With regard to surgical technique, overall, there was 15.2 MME less prescribed and an average of 1.03 MME more remaining on the first postoperative visit with endoscopic CTR compared with open CTR. Patient satisfaction with postoperative pain control between endoscopic and open release demonstrated an average score of 7.1 and 8.0, respectively. CONCLUSIONS: This study demonstrates minimal differences in opioid pain medication use, patient satisfaction with postoperative pain control, and pain scores in a comparison of surgical technique as well as anesthesia type. TYPE OF STUDY/LEVEL OF EVIDENCE: Therapeutic IV.


Assuntos
Síndrome do Túnel Carpal , Anestesia Local , Anestésicos Locais , Síndrome do Túnel Carpal/cirurgia , Humanos , Dor Pós-Operatória/prevenção & controle , Estudos Prospectivos
2.
Arthroscopy ; 32(8): 1544-1550.e1, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27107906

RESUMO

PURPOSE: To compare the analgesic efficacy of 3-day continuous interscalene brachial plexus block versus a single-shot block for arthroscopic rotator cuff repair. METHODS: Eighty-five patients scheduled for arthroscopic rotator cuff repair were randomly assigned to either the single-shot group (SSG) or continuous interscalene brachial block group (CG). Patients in the SSG received 2.5 mg/kg of 0.5% bupivacaine up to 25 mL; the CG received the same dose as a loading dose via catheter followed by an infusion of 0.125% bupivacaine at 5 mL/h and a patient-controlled bolus of 5 mL hourly for 72 hours. Follow-up after discharge was with telephone calls over the next 3 days. Pain was measured on a visual analog scale. Also measured were sleep disturbance, number of opioid doses taken, adverse effects, and level of patient satisfaction. RESULTS: The median rest pain scores on the 3 days of follow-up measured on a scale of 0 to 10 (with 10 equal to greatest pain) were 0, 0, and 3 in the CG compared with 4, 4, and 3 in the SSG (P < .001) for days 1, 2, and 3, respectively. The median maximum scores were 2, 2, and 4 in the CG compared with 8, 7, and 6 in the SSG (P < .001) for the same time period. CONCLUSIONS: A 3-day continuous interscalene brachial plexus block provides better analgesia than a single-shot block. Sleep patterns were better, and less opioid was needed after arthroscopic rotator cuff repair in patients given a continuous plexus block. LEVEL OF EVIDENCE: Level I, prospective randomized study.


Assuntos
Analgésicos Opioides/uso terapêutico , Anestésicos Locais/administração & dosagem , Artroscopia/métodos , Bloqueio do Plexo Braquial/métodos , Bupivacaína/administração & dosagem , Dor Pós-Operatória/tratamento farmacológico , Lesões do Manguito Rotador/cirurgia , Adulto , Idoso , Procedimentos Cirúrgicos Ambulatórios , Anestesia Local , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Manejo da Dor , Medição da Dor , Estudos Prospectivos
3.
J Hand Surg Glob Online ; 5(4): 407-412, 2023 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-37521540

RESUMO

Purpose: Discrepancies exist between previous biomechanical and clinical studies when determining acceptable metacarpal shortening after metacarpal fractures. This study aimed to determine the amount of acceptable shortening after a metacarpal fracture before finger motion and strength is compromised. Methods: We defrosted ten fresh-frozen cadaveric hands. A screw-driven external fixator was placed to stabilize the metacarpal, then a 15.0-mm section of the index metacarpal was excised and replaced with a three dimensional-printed, custom-designed polyethylene insert. The hand was then mounted on a custom testing rig, and the index finger was flexed using the flexor digitorum profundus tendon. Joint angles and fingertip force were recorded as the finger was flexed. Incrementally smaller inserts were placed, and testing was repeated. Results: The average joint angles of the intact condition for the metacarpophalangeal, proximal interphalangeal, and distal interphalangeal joints were (54 [SD = 13], 79 [SD = 21], and 73 [SD = 10]), respectively. There were no statistically significant changes to any joint angle with any amount of shortening. The maximal fingertip contact pressures were 41 N (17), 31 N (12), 24 N (14), 19 N, (11), and 14 N (8) for the 15 mm, 12.5 mm, 10 mm, 7.5 mm, and 5 mm inserts, respectively. All changes in fingertip force by insert size were statistically significant. Conclusion: Metacarpal shortening does not affect flexion range of motion regardless of the amount of shortening, but it significantly affects finger strength. The loss of strength after shortening was approximately 6.5% per mm of shortening for the fractured metacarpal. Clinical Relevance: When viewed in the context of the hand as a whole and the contribution of the index finger to grip being only 23.5%, it is unlikely that any shortening will significantly affect the average patient regarding grip strength. However, for a patient who requires fine motor strength, any amount of shortening may affect their finger function and needs to be addressed.

4.
J Hand Surg Glob Online ; 4(4): 214-219, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35880149

RESUMO

Purpose: We investigated unique tendon growth-factor expression profiles over time in response to simultaneous, similar injuries. Characterizing these genetic differences lays the foundation for creating targeted, tendon-specific therapies and provides insight into why current growth-factor treatments have success in some applications but not others. Methods: The left fourth digital flexor, triceps, and supraspinatus tendons in 24 rats were cut to 50% of their transverse width at the midbelly under anesthesia. On postoperative days 1, 3, 5, 7, and 14, randomly selected rats were sacrificed, and the damaged tendons were excised and flash-frozen in liquid nitrogen. The expressional fibroblast growth factor 1, bone morphogenic protein 13, and transforming growth factor ß-1 were measured at each time point and compared to their respective, uninjured levels with real-time polymerase chain reaction. Results: The digital flexor tendon showed exponentially elevated expression of all 3 factors over the preinjury baseline values. Expression in the triceps and supraspinatus had more variation over time. The triceps tendon showed a considerable decrease of transforming growth factor ß-1 and bone morphogenic protein 13 expression. The supraspinatus tendon had statistically significant increases of both transforming growth factor ß-1 and bone morphogenic protein 13 expression relative to preoperative, uninjured levels, with a nonstatistically significant decrease of fibroblast growth factor 1. Conclusions: Our study suggests different tendons express their own unique growth-factor profiles after similar, simultaneous injuries. The digital flexor showed particularly high, sustained levels of growth-factor expression in comparison to the supraspinatus and triceps, suggesting that variable dosing may be necessary for growth-factor therapies aimed at supplementing innate responses in these different tendon types. Clinical relevance: These data show different tendons express unique trends of growth-factor expression over time in response to injury, suggesting each unique tendon may require specific dosing or knockdown therapies. These observations serve as a foundation for more tendon-specific questioning, experimentation, and therapeutic design.

5.
J Hand Surg Am ; 35(1): 11-8, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20117303

RESUMO

PURPOSE: The aims of this study were to examine nonrepaired 90% partial lacerations of human cadaver flexor digitorum profundus (FDP) tendon after simulated active motion, and to assess the residual ultimate tensile strength. METHODS: Partial, transverse zone II flexor tendon lacerations were made in the volar 90% of the tendon substance in 10 FDP tendons from 5 fresh-frozen human cadaver hands. The tendons were cycled in the curvilinear fashion described by Greenwald 500 times to a tension 25% greater than the maximum in vivo active FDP flexion force measured by Schuind and colleagues. The tendons were then loaded to failure using the same curvilinear model. RESULTS: No tendons ruptured during cycling. Triggering occurred in 3 tendons. All 3 began triggering early in the cycling process, and continued to trigger throughout the remainder of the 500 cycles. The observed triggering mechanics in each case involved the interaction of the proximal face of the lacerated tendon with Camper's chiasm and the pulley edges during extension. The load to failure value of the 90% partially lacerated tendons averaged 141.7 +/- 13 N (mean +/- standard deviation). Tendon failure occurred by delamination of the intact collagen fibers from the distal, discontinuous 90% of the tendon. CONCLUSIONS: Cadaveric transverse zone II partial flexor tendon lacerations have residual tensile strength greater than the force required for protected active mobilization.


Assuntos
Traumatismos da Mão/fisiopatologia , Lacerações/fisiopatologia , Traumatismos dos Tendões/fisiopatologia , Fenômenos Biomecânicos , Cadáver , Humanos , Movimento/fisiologia , Resistência à Tração
6.
J Clin Orthop Trauma ; 10(2): 422-426, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30828219

RESUMO

Implant removal comprises 5% of all orthopaedic surgery procedures performed annually. Surgical indications range from implant failure, infection, non-union, and symptomatic hardware. Intra-operatively, surgeons need to prepare for complications including bony overgrowth, cold-welding, broken screws, and stripped screw heads. Large anatomic dissections required for complete hardware removal place the patient at increased risk of complications due to increased operating time and larger dissections. The authors present a safe and effective technique for the partial removal of surgical implants. The technique utilizes a high-speed burr to cut surgical plates, minimizing the total dissection and operative time. Sterile surgical laps covered in water-based lubricant capture metal debris to reduce tissue contamination as well as surgeon exposure to metal particles.

7.
J Am Acad Orthop Surg ; 25(8): e175-e184, 2017 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-28665804

RESUMO

INTRODUCTION: In-hospital outcomes were compared among patients with shoulder septic arthritis treated with arthrocentesis, open irrigation and débridement, or arthroscopic irrigation and débridement. METHODS: The Nationwide Inpatient Sample database was queried for all cases of native shoulder septic arthritis between 2002 and 2011. Patient demographics, comorbidities, and hospitalization complications were compared for the shoulder arthrocentesis (nonsurgical) and open or arthroscopic irrigation and débridement (surgical) groups. RESULTS: Data for 7,145 patients were analyzed. Medical comorbidities and complications were more common in the nonsurgical group than in the open surgical group (septicemia, 36.7% versus 23.6%, P < 0.001; death, 6.5% versus 2.5%, P < 0.001; pneumonia, 11.3% versus 6.2%, P < 0.001; septic shock, 4% versus 2.2%, P < 0.001; and urinary tract infection, 15.5% versus 10.2%, P < 0.001). The mean length of stay was longer in the nonsurgical group compared with the open surgical group (11.5 days versus 10.5 days, respectively; P = 0.002) and the percentage of patients discharged to home was lower (55.1% versus 64.0%, respectively; P < 0.001). Compared with the open surgical group, the arthroscopic surgical group had higher incidences of perioperative septicemia and urinary tract infection and similar average length of stay, hospital charges, and blood transfusion rates, but a lower incidence of osteomyelitis (P < 0.001). In a subgroup of patients with septicemia, Staphylococcus aureus was the most frequently cultured causative organism. DISCUSSION: Septic arthritis in the shoulder is challenging to manage, and patients often have medical comorbidities and complications. In this study, the nonsurgically treated patients had substantially more preexisting comorbidities and in-hospital complications than the surgically treated patients had, which likely contributed to the longer average length of stay and lower discharge percentage in the nonsurgical group. CONCLUSION: Patients with septic arthritis of the shoulder frequently experience substantial systemic complications regardless of the treatment method. Septicemia was a common complication among all treatment groups, with cultures most frequently indicating Staphylococcus aureus as the causative organism. LEVEL OF EVIDENCE: Therapeutic level III.


Assuntos
Artrite Infecciosa/terapia , Articulação do Ombro , Artroscopia , Comorbidade , Desbridamento , Humanos , Estudos Retrospectivos , Sepse/etiologia , Infecções Estafilocócicas/etiologia , Staphylococcus aureus
9.
Hand Clin ; 21(2): 167-71, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15882595

RESUMO

Zone I flexor tendon injuries entail injuries to the flexor digitorum profundus (FDP) tendon. These injuries occur distal to the superficialis insertion over the middle phalanx or proximal distal phalanx, and as such are isolated injuries to the FDP. The mechanism most commonly is closed avulsion from the distal phalanx or a laceration, but other mechanisms such as open avulsion or crush injury can occur. On physical examination, the cascade of the fingers will be disrupted, and distal interphalangeal (DIP) joint flexion must be isolated to determine if the FDP tendon is continuous, as other mechanisms are present to enable finger flexion at the other joints.


Assuntos
Traumatismos dos Dedos/cirurgia , Traumatismos dos Tendões/cirurgia , Humanos , Procedimentos Ortopédicos , Traumatismos dos Tendões/classificação
10.
Hand Clin ; 21(2): 173-9, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15882596

RESUMO

Flexor tendon repair in zone II is still a technically demanding procedure, but the outcomes have become more predictable and satisfying. Of keystone importance for obtaining the goals of normal strength and gliding of repaired flexor tendons are an atraumatic surgical technique, an appropriate suture material, a competent pulley system, and the use of early motion rehabilitation protocols. The overall goal of hand and finger function also implies timely addressing of neurovascular injuries. New devices such as the TenoFix (Ortheon Medical; Winter Park, Florida) have shown adequate strength in the laboratory but are bulky and untested for work of flexion. Insufficient clinical data and high cost may prevent widespread use.


Assuntos
Traumatismos da Mão/cirurgia , Traumatismos dos Tendões/cirurgia , Moldes Cirúrgicos , Humanos , Anamnese , Procedimentos Ortopédicos/métodos , Exame Físico , Contenções , Traumatismos dos Tendões/classificação
11.
Hand Clin ; 21(2): 267-73, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15882605

RESUMO

Clinical outcomes following flexor tendon repair have made significant improvements in the last 50 years. In that time standard treatment has evolved from secondary grafting to primary repair with postoperative rehabilitation protocols. Unfortunately, excellent results are not yet attained universally following treatment. Improving understanding of tendon healing at the cellular, molecular, and genetic levels will likely enable surgeons to modulate the normal repair process. We now look toward biologic augmentation of flexor tendon repairs to address the problems of increasing tensile strength while reducing adhesion formation following injury and operative repair.


Assuntos
Traumatismos da Mão/cirurgia , Procedimentos Ortopédicos/tendências , Traumatismos dos Tendões/cirurgia , Terapia Genética , Humanos , Transplante de Células-Tronco Mesenquimais , Biologia Molecular , Procedimentos Ortopédicos/métodos , Engenharia Tecidual
12.
Am J Sports Med ; 42(12): 2877-87, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25193888

RESUMO

BACKGROUND: Although platelet-rich plasma (PRP) is used clinically to augment tendon healing, bone morphogenetic protein-13 (BMP13) may provide a better therapeutic avenue to improve early tendon healing and repair. HYPOTHESIS: Exogenous expression of BMP13 in tenocytes will up-regulate genes involved in tendon healing. Direct delivery of adenovirus-mediated BMP13 (AdBMP13) into the injured rat supraspinatus tendon will increase biomechanical properties. STUDY DESIGN: Controlled laboratory study. METHODS: Exogenous expression of BMP13 and the major growth factors in PRP (transforming growth factor-ß1 [TGF-ß1], vascular endothelial growth factor-A [VEGF-A], and platelet-derived growth factor-BB [PDGF-BB]) was accomplished by using recombinant adenoviral vectors. The expression of tendon- and matrix-associated genes in growth factor-treated tenocytes was analyzed by use of semiquantitative reverse-transcription polymerase chain reaction. A total of 32 rats with supraspinatus defect were divided into 4 groups and injected with adenovirus-containing green fluorescent protein (AdGFP; negative control), PRP, AdBMP13, or PRP+AdBMP13. All rats were sacrificed at 2 weeks after surgery, and tendons were harvested for biomechanical testing and histologic analysis. RESULTS: BMP13 up-regulated type III collagen expression compared with AdGFP control and PRP growth factors (P < .01). BMP13 and PRP growth factors each up-regulated fibronectin expression (P < .01). There was an increase in stress to failure in each of the 3 treatment groups (P < .05 for PRP; P < .01 for AdBMP13 or PRP+AdBMP13) compared with AdGFP control. AdBMP13 demonstrated higher stress to failure than did the PRPs (P < .01). The addition of PRP did not increase the BMP13-enhanced stress to failure or stiffness. The biomechanical results were further supported by histologic analysis of the retrieved samples. CONCLUSION: Exogenous expression of BMP13 enhances tendon healing more effectively than PRP as assessed by tendon- and matrix-associated gene expression, biomechanical testing, and histologic analysis. CLINICAL RELEVANCE: While PRP is used in the clinical setting, BMP13 may be explored as a superior biofactor to improve rotator cuff tendon healing and reduce the incidence of retears.


Assuntos
Proteínas Morfogenéticas Ósseas/metabolismo , Plasma Rico em Plaquetas , Manguito Rotador/cirurgia , Cicatrização/fisiologia , Adenoviridae/metabolismo , Animais , Becaplermina , Colágeno Tipo III/metabolismo , Fibronectinas/metabolismo , Proteínas de Fluorescência Verde/metabolismo , Masculino , Microscopia , Modelos Animais , Proteínas Proto-Oncogênicas c-sis/genética , Proteínas Proto-Oncogênicas c-sis/metabolismo , Ratos Sprague-Dawley , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Manguito Rotador/patologia , Lesões do Manguito Rotador , Estresse Mecânico , Fator de Crescimento Transformador beta1/genética , Fator de Crescimento Transformador beta1/metabolismo , Regulação para Cima , Fator A de Crescimento do Endotélio Vascular/genética , Fator A de Crescimento do Endotélio Vascular/metabolismo
13.
Hand Clin ; 29(2): 295-309, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23660065

RESUMO

This article summarizes select multinational early motion protocols. Included are flexor and extensor protocols for digital tendon repair in many forms. Custom orthosis design, exercise regimens, and advanced techniques are examples of what to expect. The goal of the article is to expose the reader to new ideas, educate regarding advanced techniques in tendon rehabilitation, and stimulate independent study to further the reader's skill set.


Assuntos
Traumatismos da Mão/reabilitação , Modalidades de Fisioterapia , Traumatismos dos Tendões/reabilitação , Deambulação Precoce , Terapia por Exercício , Traumatismos da Mão/cirurgia , Humanos , Aparelhos Ortopédicos , Traumatismos dos Tendões/cirurgia
15.
J Hand Surg Am ; 32(3): 310-7, 2007 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-17336836

RESUMO

PURPOSE: Osteoarthritis of the trapeziometacarpal (TMC) joint of the thumb affects as many as 25% of postmenopausal women and 5% of middle-aged men. This study investigated the relative contribution of the dorsoradial ligament (DRL) and the deep anterior oblique ligament (dAOL) to the stability of the TMC joint. This knowledge will improve our understanding of the pathomechanics of osteoarthritis at the base of the thumb and may help to design novel reconstructive surgical procedures. METHODS: Seventeen intact cadaver hands were dissected to reveal the DRL and dAOL. Either the DRL or dAOL was transected randomly, physiologic muscle loads were applied to simulate lateral key pinch or thumb opposition, or a 3-dimensional magnetic tracking system was used to record the position of the thumb metacarpal relative to the trapezium. The differences in the 3-dimensional positions between the control and transected states were determined. RESULTS: In lateral pinch, transection of the DRL resulted in a mean increased 3-dimensional translation of 1.3 mm, whereas transection of the dAOL resulted in a mean increased 3-dimensional translation of 0.6 mm. Statistically significant 2-dimensional findings in lateral pinch after transecting the DRL or dAOL included an increased palmar subluxation of 0.3 mm and 0.2 mm, an increased radial (1.0 mm) and ulnar (0.3 mm) translation, and an increased pronation of 4.1 degrees and 2.4 degrees, respectively. In thumb opposition, transection of the DRL resulted in a mean increased 3-dimensional translation of 1.0 mm, whereas transection of the dAOL resulted in a mean increased 3-dimensional translation of 0.5 mm. CONCLUSIONS: In most degrees of freedom of metacarpal movement relative to the trapezium, the DRL is relatively more important than the dAOL in providing stability to the TMC joint.


Assuntos
Articulações dos Dedos/fisiologia , Ligamentos Articulares/fisiologia , Polegar/fisiologia , Articulações dos Dedos/fisiopatologia , Humanos , Instabilidade Articular/fisiopatologia , Ligamentos Articulares/fisiopatologia , Osteoartrite/fisiopatologia , Força de Pinça/fisiologia , Distribuição Aleatória , Técnicas de Sutura , Polegar/fisiopatologia
16.
J Hand Surg Am ; 31(8): 1272-8, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-17027786

RESUMO

PURPOSE: Coronoid injuries are classified according to the size of the coronoid fracture. The purpose of this study was to provide a detailed anatomic description of the coronoid process, with specific focus on the coronoid height, the coronoid width, and the olecranon-coronoid angle. METHODS: Thirty-five cadaveric arms were dissected. All soft tissue was removed and the ulna was disarticulated from the humerus, radius, and the carpal bones. A 3-dimensional digitizing system was used to locate 19 anatomic landmarks on each specimen. By using the 3-dimensional coordinates of the landmarks, the coronoid heights, proximal ulnar widths, and olecranon-coronoid angles were determined. RESULTS: The coronoid height, with its base defined by the trough of the trochlear notch and the slope change of the distal coronoid process, measured 15 mm and was 42% of the ulnar height. The coronoid height, with its base defined by the transverse groove of the sigmoid notch at the guiding ridge and the distal insertion of the brachialis muscle, measured 15 mm and was 43% of the ulnar height. The olecranon-coronoid angle ranged between 33 degrees and 38 degrees . CONCLUSIONS: For lateral radiographic classification of coronoid fractures, coronoid height is best defined by the trough of the trochlear notch and the slope change of the distal coronoid process. For anatomic studies, coronoid height is best defined by the transverse groove of the sigmoid notch at the guiding ridge and the distal insertion of the brachialis muscle. The olecranon-coronoid angle is best defined by the angle formed by the lines from the olecranon tip through the coronoid tip and through the slope change of the distal coronoid process. The coronoid anatomy measurements reported in this study may help to improve coronoid fracture classification.


Assuntos
Ulna/anatomia & histologia , Idoso , Idoso de 80 Anos ou mais , Cadáver , Articulação do Cotovelo/anatomia & histologia , Feminino , Humanos , Imageamento Tridimensional , Masculino , Pessoa de Meia-Idade , Caracteres Sexuais
17.
J Hand Ther ; 18(2): 87-92; quiz 93, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-15891965

RESUMO

Tendon injuries continue to be a vexing clinical problem faced by the upper extremity surgeon. These injuries often lead to disability and dissatisfaction for both the patient and treating physician. Advances throughout the century have focused largely on improvements in surgical techniques and therapy protocols. Recently, attention has been turned to the biologic pathways by which tendons heal and the growth factors involved in this process have been identified. There is great hope that these growth factors may one day be used to treat tendon injuries. This article provides a summary of the use of growth factors on tendon injuries including contemporary research, delivery methods, and future directions in this field.


Assuntos
Proteínas Morfogenéticas Ósseas/uso terapêutico , Substâncias de Crescimento/uso terapêutico , Traumatismos dos Tendões/terapia , Animais , Proteínas Morfogenéticas Ósseas/genética , Técnicas de Transferência de Genes , Terapia Genética , Inibidores do Crescimento/uso terapêutico , Substâncias de Crescimento/genética , Humanos , Aderências Teciduais/prevenção & controle , Cicatrização/fisiologia
18.
J Hand Surg Am ; 30(3): 448-54, 2005 May.
Artigo em Inglês | MEDLINE | ID: mdl-15925150

RESUMO

PURPOSE: To compare the maximum tensile load, change in work of flexion, and gapping at the repair site after zone II flexor digitorum profundus tendon repairs using 2-0, 3-0, and 4-0 braided polyester 4-strand locked cruciate repair technique in fresh-frozen cadaveric hands with standard 6-0 suture epitenon repairs, to determine which suture size is the best for a core repair. METHODS: A randomized study was designed using 41 tendons from 15 fresh-frozen cadaveric hands. We included only the flexor digitorum profundus tendons from the index, middle, and ring fingers to minimize variation between digits. Core suture size was randomized for each finger. A sharp laceration through the flexor digitorum profundus in zone II was made and a 4-strand locked cruciate braided polyester core stitch was performed along with a locked epitenon stitch. Cyclic loading was performed for 1,000 cycles. For each tendon the mean work of flexion (before/after zone II repair) and maximum tensile load were measured using a custom-designed tensiometer, as was gapping before maximum tensile loading. RESULTS: Mean gaps after 1,000 load-unload cycles to 3.9 N of pulp pinch did not approach the clinically significant limit of 3 mm in each group. By using a regression model, we found that the prerepair and postrepair comparisons for mean work of flexion to a 3.9-N pulp pinch showed the greatest change in work of flexion for 2-0 braided polyester. Statistical significance was found between 2-0 braided polyester and 3-0 or 4-0 braided polyester; however, the work of flexion between the 3-0 and 4-0 polyester was not clinically significant. The highest maximum tensile load was obtained with suture size 2-0 braided polyester. The maximal tensile load statistically showed 2-0 braided polyester to be stronger than 4-0 braided polyester but we found no statistically significant difference between 3-0 and 2-0 braided polyester or between 3-0 and 4-0 braided polyester. CONCLUSIONS: In this cadaveric study we found that increasing locking cruciate suture caliber from 4-0 to 2-0 increased maximum tensile strength but also caused increased work of flexion. Gapping was not affected by suture caliber. There was no significant difference in strength or mean change in work of flexion between 3-0 or 4-0 braided polyester sutures.


Assuntos
Lacerações/cirurgia , Teste de Materiais , Suturas , Traumatismos dos Tendões/cirurgia , Tendões/cirurgia , Fenômenos Biomecânicos , Cadáver , Desenho de Equipamento , Humanos , Nylons , Poliésteres , Polietilenotereftalatos
19.
J Hand Surg Am ; 30(1): 136-41, 2005 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-15680569

RESUMO

PURPOSE: Adenoviral vector-based gene therapy is a promising technique for the delivery of growth factors to tendons. The objective of this study was to determine whether rabbit flexor tendons could be transduced effectively by adenoviral vectors and whether the introduction of adenoviral vectors would cause a notable local inflammatory response. METHODS: Recombinant adenoviruses expressing green fluorescent protein (AdGFP) or BMP-13 (AdBMP-13) were constructed and 3 different viral titers (1 x 10(7), 1 x 10(8), and 1 x 10(9)) were tested in this study. The second through fifth tendons of the forepaws and hindpaws of a New Zealand white rabbit were identified surgically and injected with different viral titers of adenoviruses. The fifth tendon was used as a control. The tendons were harvested 12 days after surgery. The retrieved tendons were sectioned to measure transgene expression, as well as for histologic evaluation. RESULTS: At all tested viral titers an efficient dose-dependent transgene expression was detected in all samples at 12 days after injection. At the highest dose the injection sites were notable for lymphocytic infiltration, suggesting that injected adenoviral vectors can illicit some local inflammatory response. Lymphocytic infiltration was much less apparent, however, in the tendons injected with lower titers of adenoviral vectors. There was no evidence of a massive inflammatory response and/or cell death. CONCLUSIONS: Our findings show that adenovirus-based gene therapy is an efficient means of gene delivery to rabbit flexor tendons. Transduction efficiency of transgenes was dose dependent across the tested titers, although adenovirus-induced inflammation was notable only at the highest titer. This indicates that efficient gene transfer without notable local inflammatory response may be achieved by using the lower titers. Although adenovirus-induced inflammation can be minimized by using lower viral titers, its impact on adhesion formation in the long term remains unknown.


Assuntos
Adenoviridae/genética , Terapia Genética , Vetores Genéticos , Tendões/metabolismo , Transdução Genética , Animais , Proteína Morfogenética Óssea 2 , Proteínas Morfogenéticas Ósseas/genética , Proteínas Morfogenéticas Ósseas/metabolismo , Genes Reporter , Proteínas de Fluorescência Verde/genética , Proteínas de Fluorescência Verde/metabolismo , Substâncias Luminescentes/metabolismo , Linfócitos/metabolismo , Coelhos , Traumatismos dos Tendões/terapia , Tendões/patologia , Fator de Crescimento Transformador beta/genética , Fator de Crescimento Transformador beta/metabolismo
20.
J Hand Surg Am ; 27(3): 508-17, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-12015728

RESUMO

To compare the biomechanical performances of six 4-strand flexor tendon repairs at zone II, we used an in situ testing model in 54 cadaver profundus tendons. The techniques studied were the modified Becker, modified double Tsuge, Lee, locked cruciate, Robertson, and Strickland. Prerepair and postrepair comparisons for work of flexion to a 3.9-N pulp pinch (equal to 12.6 N tendon force) showed the greatest interference to gliding in the modified Becker repair and the least in the modified double Tsuge repair. Mean gaps after 1,000 load-unload cycles to a 3.9-N pulp pinch did not approach the clinically important limit of 3 mm in all groups. Ultimate tensile strength was highest in the modified Becker (69.4 +/- 8.2 N) but not significantly higher than the modified double Tsuge (60.3 +/-15.3 N) and locked cruciate (64.1 +/- 16.2 N). In all repair groups the mean pulp pinch forces upon failure were well above values recommended for active mobilization protocols that use external load guides. The locked cruciate, modified double Tsuge, and modified Becker repairs were strong enough for an early active motion protocol after surgery. Locked cruciate and modified double Tsuge were easier to perform and provided less interference to tendon gliding than the modified Becker repair.


Assuntos
Fenômenos Biomecânicos , Técnicas de Sutura , Tendões/cirurgia , Cadáver , Articulações dos Dedos , Humanos , Procedimentos de Cirurgia Plástica/métodos , Sensibilidade e Especificidade , Traumatismos dos Tendões , Resistência à Tração
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