[The self-rating of life quality by workers of metal-processing industry].

The cross-sectional study of subjective assessment of quality of life of 300 employees of main professions of metalworking industrial production working in harmful labor environment (classes 3.1-3.4) was carried out using validated Russian version of the WHO QOL-BREF questionnaire. The software Statistica 10 was applied for statistical analysis. The statistical processing of study results was performed by calculating arithmetic mean (M), standard deviation (SD) and presented as M±SD. The non-parametric statistical methods were applied to compare two independent samples: the Mann-Whitney U criterion, the Spearman rank correlation coefficient. The data differences and correlations between the data at p<0.05 were considered statistically significant. Based on the results of analysis, satisfaction of employees with quality of life across all domains was established. The quality of life indicators were independent of gender of respondents. With age and experience, decrease of indicators for all domains was established. High indicators for physical health domain and subjective assessment of health status in examined group can be explained from the standpoint of the "healthy worker effect", but concealment of information about true state of health cannot be ruled out. The metal industry workers working in harmful labor conditions are conditionally healthy and active. There is a need for additional research to obtain objective assessment of their health status.

[The role of nutrigenetics and nutrigenomics in the prophylaxis of chronic non-communicable diseases].

Over the past several decades there has been an increase in the number of chronic noncommunicable diseases worldwide largely due to changes in diet and lifestyle, as well as exposure to adverse environmental factors. The so-called omics technologies (genomic, proteomic, metabolomic and transcriptomic) are used as tools for comprehensive analysis and monitoring of human health. Currently, genomic and post-genomic technologies are used to study the effects of various nutrients on human health. The purpose of the review was to summarize and analyze modern omics technologies used in the prevention of non-communicable diseases associated with human dietary habits. Material and methods. The literature search was carried out using PubMed, eLibrary, ResearchGate and ScienceDaily databases using the keywords "nutrigenetics", "nutrigenomics", "SNP", as well as the names of specific factors, genes and diseases. Results. The review provides up-to-date information on the role of knowledge of nutrigenetics and nutrigenomics in the prevention of chronic non-communicable diseases. Examples of the influence of specific single-nucleotide polymorphisms and genetic variations on various aspects of nutrition are given, from which recommendations for correcting the diet of carriers of these alleles to reduce the risk of cardiovascular diseases, type 2 diabetes mellitus, and obesity follow. Examples of nutrient influence on gene expression are also given and some genetic markers of metabolic disorders which can lead to diseases such as type 2 diabetes mellitus, obesity, inflammatory diseases of the colon, cardiovascular and neurodegenerative diseases, cancer are listed. Recommendations are given on the practical use of the knowledge gained during nutrigenomic studies on the effect of nutrient intake on the risk of non-communicable diseases for their prevention. Conclusion. The practical use of omics technologies can provide a more effective prevention of non-communicable diseases, contributing to an increase in the quality of life and the preservation of labor longevity of the population.

Supramolecular complexes of the Agrobacterium tumefaciens virulence protein VirE2.

Virulence protein VirE2 from Agrobacterium tumefaciens is involved in plant infection by transferring a fragment of agrobacterial Ti plasmid ssT-DNA in complex with VirE2-VirD2 proteins into the plant cell nucleus. The VirE2 protein interactions with ssDNA and formation of VirE2 protein complexes in vitro and in silico have been studied. Using dynamic light scattering we found that purified recombinant protein VirE2 exists in buffer solution in the form of complexes of 2-4 protein molecules of 12-18 nm size. We used computer methods to design models of complexes consisting of two and four individual VirE2 proteins, and their dimensions were estimated. Dimensions of VirE2 complexes with ssDNA (550 and 700 nucleotide residues) were determined using transmission electron microscopy and dynamic light scattering. We found that in vitro, upon interaction with ssDNA recombinant protein, VirE2 is able to alter conformation of the latter by shortening the initial length of the ssDNA.