RESUMO
Opioid analgesia, the selective suppression of pain without effects on other sensations, also distinguishes between different types of pain: severe, persistent pain is potently inhibited by opioids, but they fail to cohceal the sensation of a pinprick. The cellular basis for this specificity was analyzed by means of patch-clamp experiments performed on fluorescently labeled nociceptive neurons (nociceptors) that innervate rat tooth pulp. Activation of the mu opioid receptor inhibited calcium channels on almost all small nociceptors but had minimal effect on large nociceptors. Somatostatin had the opposite specificity, preferentially inhibiting calcium channels on the large cells. Because persistent pain is mediated by slow-conducting, small nociceptors, opioids are thus likely to inhibit neurotransmitter release only at those primary synapses specialized for persistent pain.
Assuntos
Analgésicos/farmacologia , Encefalinas/farmacologia , Neurônios Aferentes/efeitos dos fármacos , Nociceptores/efeitos dos fármacos , Receptores Opioides mu/fisiologia , Animais , Canais de Cálcio/efeitos dos fármacos , Células Cultivadas , Polpa Dentária/inervação , Ala(2)-MePhe(4)-Gly(5)-Encefalina , Masculino , Neurônios Aferentes/fisiologia , Neurotransmissores/metabolismo , Nociceptores/fisiologia , Técnicas de Patch-Clamp , Terminações Pré-Sinápticas/efeitos dos fármacos , Terminações Pré-Sinápticas/metabolismo , Ratos , Ratos Sprague-Dawley , Receptores de Somatostatina/fisiologia , Bloqueadores dos Canais de Sódio , Somatostatina/farmacologiaRESUMO
Multiple types of calcium channels have been found in neurons, but uncertainty remains about which ones are involved in stimulus-secretion coupling. Two types of calcium channels in rat sympathetic neurons were described, and their relative importance in controlling norepinephrine release was analyzed. N-type and L-type calcium channels differed in voltage dependence, unitary barium conductance, and pharmacology. Nitrendipine inhibited activity of L-type channels but not N-type channels. Potassium-evoked norepinephrine release was markedly reduced by cadmium and the conesnail peptide toxin omega-Conus geographus toxin VIA, agents that block both N- and L-type channels, but was little affected by nitrendipine at concentrations that strongly reduce calcium influx, as measured by fura-2. Thus N-type calcium channels play a dominant role in the depolarization-evoked release of norepinephrine.
Assuntos
Cálcio/fisiologia , Gânglios Simpáticos/fisiologia , Canais Iônicos/fisiologia , Norepinefrina/metabolismo , Animais , Bloqueadores dos Canais de Cálcio/farmacologia , Condutividade Elétrica , Técnicas In Vitro , Potenciais da Membrana , Ratos , Taxa Secretória/efeitos dos fármacosRESUMO
High-threshold Ca2+ current triggers neurotransmitter release, but the existence, significance, and correct identification of different types of high-threshold Ca2+ channels remain controversial. We show selective inhibition of a rapidly inactivating component of high-threshold Ca2+ current in rat sensory neurons by bursts of brief pulses that mimic trains of action potentials and by prolonged depolarization just above the normal rest potential. In contrast, a slowly inactivating component decreases only when sufficient Ca2+ accumulates within the cell. Thus, there are physiologically important differences: whereas availability of the transient component depends on the value of the rest potential and the pattern of a prior stimulus, the sustained component seems to provide a baseline level of voltage-dependent Ca2+ entry that is lost only when intracellular Ca2+ rises.
Assuntos
Cálcio/fisiologia , Neurônios Aferentes/fisiologia , ômega-Conotoxinas , Animais , Bloqueadores dos Canais de Cálcio/farmacologia , Limiar Diferencial , Eletrofisiologia , Matemática , Peptídeos Cíclicos/farmacologia , Fatores de TempoRESUMO
Opioids and opiates decrease the duration of action potentials and the amount of neurotransmitter released from sensory neurons. The mu-type opioid receptor, the binding site for morphine, is thought to act exclusively on K+ channels. Here, we show that activation of the mu receptor inhibits Ca2+ channels in rat sensory neurons; the effect is blocked by a mu antagonist and is not mimicked by kappa or delta receptor agonists. Both low-threshold (T-type) and high-threshold Ca2+ currents are partially suppressed. omega-Conotoxin-sensitive and omega-conotoxin-insensitive, high-threshold Ca2+ currents are inhibited. The kinetic effect on high-threshold current is like that caused by diminished rest potential: the transient component is selectively lost, whereas the sustained component is spared.
Assuntos
Canais de Cálcio/fisiologia , Receptores Opioides/fisiologia , (trans)-Isômero de 3,4-dicloro-N-metil-N-(2-(1-pirrolidinil)-ciclo-hexil)-benzenoacetamida , Sequência de Aminoácidos , Animais , Canais de Cálcio/efeitos dos fármacos , Células Cultivadas , Condutividade Elétrica , Ala(2)-MePhe(4)-Gly(5)-Encefalina , D-Penicilina (2,5)-Encefalina , Encefalinas/farmacologia , Dados de Sequência Molecular , Venenos de Moluscos/farmacologia , Naltrexona/análogos & derivados , Naltrexona/farmacologia , Antagonistas de Entorpecentes , Pirrolidinas/farmacologia , Ratos , Receptores Opioides/efeitos dos fármacos , Receptores Opioides delta , Receptores Opioides kappa , Receptores Opioides mu , ômega-Conotoxina GVIARESUMO
Lactic acid produced by anaerobic metabolism during cardiac ischemia is among several compounds suggested to trigger anginal chest pain; however, the pH reached when a coronary artery is occluded (pH 7.0 to 6.7) can also occur during systemic acidosis, which causes no chest pain. Here we show that lactate, acting through extracellular divalent ions, dramatically increases activity of an acid-sensing ion channel (ASIC) that is highly expressed on sensory neurons that innervate the heart. The effect should confer upon neurons that express ASICs an extra sensitivity to the lactic acidosis of local ischemia compared to acidity caused by systemic pathology.
Assuntos
Ácidos/metabolismo , Canais de Cálcio/fisiologia , Isquemia/fisiopatologia , Ácido Láctico/metabolismo , Neurônios Aferentes/fisiologia , Animais , Células COS , Relação Dose-Resposta a Droga , Concentração de Íons de Hidrogênio , Ácido Láctico/farmacologia , Músculo Esquelético/irrigação sanguínea , Neurônios/fisiologia , Neurônios Aferentes/efeitos dos fármacos , Técnicas de Patch-Clamp , RatosRESUMO
Every organism with a nervous system can detect changes in temperature. Recent studies on sensory neurons from rats and genetic evidence from nematodes have provided intriguing hints about the molecular basis of thermosensation.
Assuntos
Regulação da Temperatura Corporal , Células Receptoras Sensoriais/fisiologia , Animais , Temperatura Alta , Humanos , Canais Iônicos/fisiologia , Neurônios/fisiologiaRESUMO
Neurotrophins promote multiple actions on neuronal cells including cell survival and differentiation. The best-studied neurotrophin, nerve growth factor (NGF), is a major survival factor in sympathetic and sensory neurons and promotes differentiation in a well-studied model system, PC12 cells. To mediate these actions, NGF binds to the TrkA receptor to trigger intracellular signaling cascades. Two kinases whose activities mediate these processes include the mitogen-activated protein (MAP) kinase (or extracellular signal-regulated kinase [ERK]) and phosphoinositide 3-kinase (PI3-K). To examine potential interactions between the ERK and PI3-K pathways, we studied the requirement of PI3-K for NGF activation of the ERK signaling cascade in dorsal root ganglion cells and PC12 cells. We show that PI3-K is required for TrkA internalization and participates in NGF signaling to ERKs via distinct actions on the small G proteins Ras and Rap1. In PC12 cells, NGF activates Ras and Rap1 to elicit the rapid and sustained activation of ERKs respectively. We show here that Rap1 activation requires both TrkA internalization and PI3-K, whereas Ras activation requires neither TrkA internalization nor PI3-K. Both inhibitors of PI3-K and inhibitors of endocytosis prevent GTP loading of Rap1 and block sustained ERK activation by NGF. PI3-K and endocytosis may also regulate ERK signaling at a second site downstream of Ras, since both rapid ERK activation and the Ras-dependent activation of the MAP kinase kinase kinase B-Raf are blocked by inhibition of either PI3-K or endocytosis. The results of this study suggest that PI3-K may be required for the signals initiated by TrkA internalization and demonstrate that specific endocytic events may distinguish ERK signaling via Rap1 and Ras.
Assuntos
Endocitose , Fator de Crescimento Neural/metabolismo , Fosfatidilinositol 3-Quinases/fisiologia , Transdução de Sinais , Proteínas rap1 de Ligação ao GTP/metabolismo , Proteínas ras/metabolismo , 1-Metil-3-Isobutilxantina/farmacologia , Animais , Western Blotting , Diferenciação Celular , Sobrevivência Celular , Células Cultivadas , Cromatografia de Afinidade , Cromonas/farmacologia , Colforsina/farmacologia , Relação Dose-Resposta a Droga , Ativação Enzimática , Inibidores Enzimáticos/farmacologia , Flavonoides/farmacologia , Gânglios Espinais/metabolismo , Imuno-Histoquímica , Luciferases/metabolismo , Microscopia Eletrônica , Microscopia de Fluorescência , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Modelos Biológicos , Morfolinas/farmacologia , Células PC12 , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Proto-Oncogênicas c-raf/metabolismo , Ratos , Receptor trkA/metabolismo , Fatores de Tempo , TransfecçãoRESUMO
An unexpected variety of different types of Ca2+ channels have been identified using molecular cloning and selective Ca2+ channel toxins. A main focus of current research in this field is to characterize these different channel types and the molecular mechanisms by which Ca2+ channels perform their basic activities of gating, selectivity and modulation. Recent advances demonstrate the roles of different types of Ca2+ channels in muscle and nerve, and provide hints about the structures involved in selectivity and gating.
Assuntos
Canais de Cálcio/fisiologia , Animais , Canais de Cálcio/química , Canais de Cálcio/classificação , Humanos , Ativação do Canal Iônico , Relação Estrutura-Atividade , Transmissão Sináptica/fisiologiaRESUMO
Voltage-gated Ca2+ channels select Ca2+ over competing, more abundant ions by means of a high affinity binding site in the pore. The maximum off rate from this site is approximately 1,000x slower than observed Ca2+ current. Various theories that explain how high Ca2+ current can pass through such a sticky pore all assume that flux occurs from a condition in which the pore's affinity for Ca2+ transiently decreases because of ion interactions. Here, we use rate theory calculations to demonstrate a different mechanism that requires no transient changes in affinity to quantitatively reproduce observed Ca2+ channel behavior. The model pore has a single high affinity Ca2+ binding site flanked by a low affinity site on either side; ions permeate in single file without repulsive interactions. The low affinity sites provide steps of potential energy that speed the exit of a Ca2+ ion off the selectivity site, just as potential energy steps accelerate other chemical reactions. The steps could be provided by weak binding in the nonselective vestibules that appear to be a general feature of ion channels, by specific protein structures in a long pore, or by stepwise rehydration of a permeating ion. The previous ion-interaction models and this stepwise permeation model demonstrate two general mechanisms, which might well work together, to simultaneously generate high flux and high selectivity in single file pores.
Assuntos
Canais de Cálcio/fisiologia , Bário/metabolismo , Bário/farmacologia , Cálcio/metabolismo , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/efeitos dos fármacos , Eletrofisiologia , Ativação do Canal Iônico/efeitos dos fármacos , Ativação do Canal Iônico/fisiologia , Modelos BiológicosRESUMO
Following purification using Ca2+ channel drugs as ligands, the skeletal muscle Ca2+ channel was shown to be a five-subunit structure containing one large (175 kDa) protein that is the pore and four auxiliary subunits. Each subunit has been cloned and expression studies are proceeding rapidly. Particular success has been made in structure-function studies of excitation-contraction coupling using a Ca2+ channel-free mutant muscle. The work confirmed the suggestion made from physiological studies that muscle Ca2+ channels serve dual roles: passing Ca2+ and triggering Ca2+ release from an intracellular organelle. A variety of other predictions about the structure of Ca2+ channels have been reviewed here and these may soon be possible to test. Such concrete predictions along with analogies to studies on other voltage-dependent ion channels should speed progress in structure-function studies of Ca2+ channels.
Assuntos
Canais de Cálcio/química , Estrutura Terciária de Proteína , Animais , Cálcio/metabolismo , Contração Muscular , Relação Estrutura-AtividadeRESUMO
Ischemic pain occurs when there is insufficient blood flow for the metabolic needs of an organ. The pain of a heart attack is the prototypical example. Multiple compounds released from ischemic muscle likely contribute to this pain by acting on sensory neurons that innervate muscle. One such compound is lactic acid. Here, we show that ASIC3 (acid-sensing ion channel #3) has the appropriate expression pattern and physical properties to be the detector of this lactic acid. In rats, it is expressed only in sensory neurons and then only on a minority (approximately 40%) of these. Nevertheless, it is expressed at extremely high levels on virtually all dorsal root ganglion sensory neurons that innervate the heart. It is extraordinarily sensitive to protons (Hill slope 4, half-activating pH 6.7), allowing it to readily respond to the small changes in extracellular pH (from 7.4 to 7.0) that occur during muscle ischemia. Moreover, both extracellular lactate and extracellular ATP increase the sensitivity of ASIC3 to protons. This final property makes ASIC3 a "coincidence detector" of three molecules that appear during ischemia, thereby allowing it to better detect acidosis caused by ischemia than other forms of systemic acidosis such as hypercapnia.
Assuntos
Isquemia/fisiopatologia , Ácido Láctico/metabolismo , Proteínas de Membrana/fisiologia , Proteínas do Tecido Nervoso/fisiologia , Neurônios Aferentes/fisiologia , Dor/fisiopatologia , Canais de Sódio/fisiologia , Canais Iônicos Sensíveis a Ácido , Animais , Concentração de Íons de Hidrogênio , RatosRESUMO
The release of cytosol from damaged cells has been proposed to be a chemical trigger for nociception. K(+), H(+), adenosine triphosphate (ATP), and glutamate are algogenic agents within cytosol that might contribute to such an effect. To examine which, if any, compounds in cytosol activate ion channels on nociceptors, we recorded currents in dissociated nociceptors when nearby skin cells were damaged. Skin cell damage caused action potential firing and inward currents in nociceptors. Extracts of fibroblast cytosol did the same. Virtually all response to extract and cell killing was eliminated by enzymatic degradation of ATP or desensitization or blockade of P2X receptors, ion channels that are activated by extracellular ATP. Thus, if cytosol provides a rapid nociceptive signal from damaged tissue, then ATP is a critical messenger and P2X receptors are its sensor.
Assuntos
Trifosfato de Adenosina/metabolismo , Citosol/metabolismo , Nociceptores/metabolismo , Células 3T3/citologia , Células 3T3/metabolismo , Potenciais de Ação/fisiologia , Animais , Morte Celular/fisiologia , Humanos , Camundongos , Neurônios Aferentes/metabolismo , Ratos , Receptores Purinérgicos P2/metabolismo , Receptores Purinérgicos P2X , Pele/citologia , Pele/lesõesRESUMO
We have shown the presence and activity of ATP-gated ion channels (P2X receptors) in nociceptive nerve endings, supporting the theory that these channels mediate some forms of nociception [Cook S.P., Vulchanova L., Hargreaves K. M., Elde R. and McCleskey E. W. (1997) Distinct ATP receptors on pain-sensing and stretch-sensing neurons. Nature 387, 505-508]. The kinetics and pharmacology of ATP-gated currents in nociceptors suggest that the channels are comprised of either homomeric or heteromeric combinations of P2X3 receptors. Consistent with the diverse nature of P2X structure, electrophysiological responses of rat tooth-pulp nociceptors fall into two distinct classes based on desensitization and recovery kinetics. Here, we quantified the dramatic differences in desensitization kinetics of transient and persistent currents. The major component of transient P2X current desensitized with a tau decay = 32 +/- 2 msec, while persistent current desensitized > 100-fold more slowly, tau decay = 4000 +/- 320 msec. Both currents recovered from desensitization in minutes: tau recovery = 4 min for transient current, and tau recovery = 0.7 +/- 0.2 min for persistent current. Persistent current recovery was often accompanied by a current "overrecovery" that averaged ca threefold magnitude prior to desensitization. Comparison of ATP current in elevated Ca2+ext also revealed differences in transient and presistent currents. In 2 mM Ca2+ext medium, decrease of Na+ext resulted in an almost complete reduction of persistent, but not transient, current. Subsequent elevation of Ca2+ext greatly increased the transient, but not persistent, current. Mechanistic explanations for either the increase in transient current magnitude by elevated Ca2+ext, or persistent current overrecovery may reflect endogenous pathways for P2X receptor modulation.
Assuntos
Trifosfato de Adenosina/fisiologia , Cálcio/fisiologia , Neurônios Aferentes/efeitos dos fármacos , Nociceptores/efeitos dos fármacos , Receptores Purinérgicos P2/efeitos dos fármacos , Animais , Cálcio/farmacologia , Polpa Dentária/efeitos dos fármacos , Polpa Dentária/fisiologia , Técnicas In Vitro , Masculino , Neurônios Aferentes/fisiologia , Nociceptores/fisiologia , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-Dawley , Receptores Purinérgicos P2/fisiologiaRESUMO
Using the whole-cell patch-clamp technique on acutely dissociated and cultured adult rat sensory neurons, we characterized the K+ currents by voltage dependence, kinetics, calcium dependence, and pharmacology. In the presence of Ca channel blockers, the cells heterogeneously expressed transient and sustained outward K+ currents. The transient current was a high-threshold A-current which activated at potentials greater than -30 mV and was blocked by 4-aminopyridine. Some of the sustained current was classified as a delayed rectifier. It demonstrated shallow voltage-dependent inactivation and was blocked by tetraethylammonium. Capsaicin produced large reductions in both transient and sustained currents with an EC50 of 8 microM. Likewise, dendrotoxin partially blocked both currents but with an EC50 of 21 nM. In the absence of Ca channel blockers, a prominent Ca-dependent K+ current was observed. The kinetics of whole-cell potassium currents varied widely among cells, perhaps reflecting the different functional properties of sensory neurons. We also investigated the effects of elevating intracellular cyclic AMP and applying opioids on K+ currents. Membrane-permanent analogs of cyclic AMP and phosphodiesterase inhibitors caused small reductions in voltage-dependent outward current. In contrast, forskolin produced a large reduction in outward current. This response was not solely mediated by cyclic AMP, since large responses were elicited with an inactive congener, 1,9-dideoxyforskolin, but not with the active, water-soluble congener, 7-deacetyl-6-[N-acetylglycyl]-forskolin. Surprisingly, opioids had no effect on resting or voltage-dependent K+ conductances. However, opioid inhibition of Ca2+ currents and Ca-dependent K+ currents was observed. The failure to demonstrate opioid modulation of resting or voltage dependent K+ currents suggests that modulation of Ca2+ currents is the principal mechanism for the inhibitory effect of opioids on sensory neurons.
Assuntos
AMP Cíclico/farmacologia , Entorpecentes/farmacologia , Neurônios Aferentes/metabolismo , Canais de Potássio/metabolismo , 4-Aminopiridina/farmacologia , Animais , Capsaicina/farmacologia , Colforsina/farmacologia , Venenos Elapídicos/farmacologia , Eletrofisiologia , Técnicas In Vitro , Ativação do Canal Iônico/efeitos dos fármacos , Cinética , Neurônios Aferentes/efeitos dos fármacos , Neurotoxinas/farmacologia , Ratos , Ratos Sprague-Dawley , Compostos de Tetraetilamônio/farmacologiaRESUMO
Though there is some evidence to the contrary, dogma claims that primary sensory neurons in the dorsal root ganglion do not interact, that the ganglion serves as a through-station in which no signal processing occurs. Here we use patch clamp and immunocytochemistry to show that sensory neurons in primary culture can form chemical synapses on each other. The resulting neurotransmitter release is calcium dependent and uses synaptotagmin-containing vesicles. On many cells studied, the postsynaptic receptor for the neurotransmitter is a P2X receptor, an ion channel activated by extracellular ATP. This shows that sensory neurons have the machinery to form purinergic synapses on each other and that they do so when placed in short-term tissue culture.
Assuntos
Neurônios Aferentes/citologia , Neurônios Aferentes/fisiologia , Receptores Purinérgicos P2/fisiologia , Sinapses/fisiologia , Animais , Células Cultivadas , Potenciais Evocados/fisiologia , Gânglios Espinais/citologia , Técnicas de Patch-Clamp , Ratos , Ratos Sprague-DawleyRESUMO
Growth of the human malaria parasite, Plasmodium falciparum, within the red blood cell (RBC) requires external Ca++ and is associated with a markedly elevated intracellular Ca++ concentration, [Ca++]i. We used 45Ca++ flux studies and patch clamp recordings to examine the mechanisms responsible for this increased [Ca++]i. The 45Ca++ flux studies indicated that net Ca++ entry into parasitized RBCs (PRBCs) is 18 times faster than into unparasitized ATPase that keeps the [Ca++]i of unparasitized RBCs exceedingly low. Acceleration of the preexisting Ca++ entry, ATPase that keeps the [Ca++] of unparasitized RBCs exceedingly low. Acceleration of the preexisting Ca++ entry, mediated by a divalent cation carrier, also cannot explain Ca++ accumulation in PRBCs: there are fundamental differences in substrate preference and in the effects of external Ca++ on 45Ca++ efflux between unparasitized RBCs and PRBCs. Patch clamp of intact PRBC surface membranes revealed rare unitary channel openings not observed on unparasitized RBCs. With 80 mM of CaCl2 in the patch pipette, this channel carried inward current, suggesting Ca++ entry at a rate comparable with the observed 45Ca++ flux. These data indicate that the malaria parasite induces a novel pathway in the host RBC membrane for Ca++ entry and suggest that this pathway is a Ca++-permeable channel.
Assuntos
Canais de Cálcio/fisiologia , Cálcio/metabolismo , Eritrócitos/metabolismo , Eritrócitos/parasitologia , Malária Falciparum/sangue , Plasmodium falciparum , Animais , ATPases Transportadoras de Cálcio/metabolismo , Humanos , Malária Falciparum/parasitologia , Especificidade por SubstratoRESUMO
We have recently published papers in which sensory neurons that innervate either the tooth pulp or masseter muscle spindles were labelled in vivo and later identified and studied in primary tissue culture (Taddese et al., 1995; Cook et al., 1997). Here, we provide detailed descriptions of cell labelling and tissue culture methods that we used. The purpose of the preparations is to compare nociceptive and non-nociceptive sensory neurons in vitro. The spindles in mastication muscles are the only muscle afferents whose cell bodies reside in the mesencephalic nucleus (MeN5) of the fifth nerve (Corbin and Harrison, J Neurophysiol, 1940; Cody et al., J Physiol, 1972). Thus, labelling neurons projecting to the masseter muscle and dissecting the MeN5 isolates muscle spindle afferents. Pain is the only conscious sensation elicited by physiological stimulus of tooth pulp (Anderson and Matthews, 1967; Edwall and Olgart, 1977; Ahlquist et al., 1984; Narhi et al., 1994); there may be unconscious sensations that arise from the pulp, but these have never been demonstrated. Thus, tooth pulp afferents represent at least a highly enriched, and possibly a pure, population of nociceptors. In broad outline, the methods of labelling and tissue culture are standard, but we have honed many details in order to obtain practical yields.
Assuntos
Técnicas de Cultura de Células/métodos , Separação Celular/métodos , Neurônios Aferentes/citologia , Marcadores de Afinidade/administração & dosagem , Animais , Carbocianinas/administração & dosagem , Células Cultivadas , Centrifugação com Gradiente de Concentração , Dissecação , Corantes Fluorescentes/administração & dosagem , Injeções Intramusculares , Músculo Masseter/inervação , Mesencéfalo/citologia , Neuroglia/citologia , Ratos , Ratos Sprague-Dawley , Soluções , Propriedades de Superfície , Germe de Dente/inervação , Gânglio Trigeminal/citologiaRESUMO
Ginseng root, a traditional oriental medicine, contains more than a dozen biologically active saponins called ginsenosides, including one present in only trace amounts called ginsenoside-Rf (Rf). Previously, we showed that Rf inhibits Ca2+ channels in mammalian sensory neurons through a mechanism requiring G-proteins, whereas a variety of other ginsenosides were relatively ineffective. Since inhibition of Ca2+ channels in sensory neurons contributes to antinociception by opioids, we tested for analgesic actions of Rf. We find dose-dependent antinociception by systemic administration of Rf in mice using two separate assays of tonic pain: in the acetic acid abdominal constriction test, the ED50 was 56+/-9 mg/kg, a concentration similar to those reported for aspirin and acetaminophen in the same assay; in the tonic phase of the biphasic formalin test, the ED50 was 129+/-32 mg/kg. Rf failed to affect nociception measured in three assays of acute pain: the acute phase of the formalin test, and the thermal (49 degrees C) tail-flick and increasing-temperature (3 degrees C/min) hot-plate tests. The simplest explanation is that Rf inhibits tonic pain without affecting acute pain, but other possibilities exist. Seeking a cellular explanation for the effect, we tested whether Rf suppresses Ca2+ channels on identified nociceptors. Inhibition was seen on large, but not small, nociceptors. This is inconsistent with a selective effect on tonic pain, so it seems unlikely that Ca2+ channel inhibition on primary sensory neurons can fully explain the behavioral antinociception we have demonstrated for Rf.
Assuntos
Analgésicos/farmacologia , Ginsenosídeos , Nociceptores/efeitos dos fármacos , Dor/tratamento farmacológico , Saponinas/farmacologia , Músculos Abdominais/fisiologia , Animais , Comportamento Animal/efeitos dos fármacos , Temperatura Corporal/efeitos dos fármacos , Bloqueadores dos Canais de Cálcio/farmacologia , Canais de Cálcio/fisiologia , Relação Dose-Resposta a Droga , Formaldeído , Temperatura Alta , Locomoção/efeitos dos fármacos , Camundongos , Neurônios Aferentes/química , Neurônios Aferentes/efeitos dos fármacos , Neurônios Aferentes/fisiologia , Panax , Técnicas de Patch-Clamp , Plantas Medicinais , CaudaRESUMO
The effect of Panax ginseng root extract on Ca2+ current of adult rat trigeminal ganglion neurons was investigated using whole-cell patch-clamp methods. The application of P. ginseng root extract (100 micrograms/ml) produced rapid, reversible reduction of the Ca2+ current by 22 +/- 4%. Treatment with pertussis toxin (250 ng/ml) for 16 h reduced the inhibition to 4 +/- 1%. The continual presence of 1 microM DAGO, a selective mu-opioid agonist that inhibits Ca2+ channels, occluded further inhibition of Ca2+ current by P. ginseng root extract. Yohimbine, phaclofen, atropine, and naloxone--antagonists of alpha 2-adrenergic, GABAB, muscarinic, and opiate receptors, respectively--did not block the inhibitory effect on Ca2+ current of P. ginseng root extract. Thus, P. ginseng root extract acts on sensory neurons through a similar pathway as mu-type opioids: both inhibit Ca2+ channels through pertussis toxin-sensitive GTP-binding proteins. However, the receptor for P. ginseng root extract is not an alpha 2-adrenergic, GABAB, muscarinic, or opioid receptor.
Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia , Entorpecentes/farmacologia , Neurônios Aferentes/efeitos dos fármacos , Panax , Extratos Vegetais/farmacologia , Plantas Medicinais , Receptores Opioides mu/fisiologia , Animais , Canais de Cálcio/efeitos dos fármacos , Canais de Cálcio/fisiologia , Ala(2)-MePhe(4)-Gly(5)-Encefalina , Encefalinas/farmacologia , Proteínas de Ligação ao GTP/fisiologia , Técnicas In Vitro , Ativação do Canal Iônico/efeitos dos fármacos , Toxina Pertussis , Extratos Vegetais/antagonistas & inibidores , Ratos , Ratos Sprague-Dawley , Receptores de Superfície Celular/antagonistas & inibidores , Receptores Opioides mu/efeitos dos fármacos , Gânglio Trigeminal/citologia , Gânglio Trigeminal/efeitos dos fármacos , Fatores de Virulência de Bordetella/farmacologiaRESUMO
Angina, the prototypic vasoocclusive pain, is a radiating chest pain that occurs when heart muscle gets insufficient blood because of coronary artery disease. Other examples of vasoocclusive pain include the acute pain of heart attack and the intermittent pains that accompany sickle cell anemia and peripheral artery disease. All these conditions cause ischemia - insufficient oxygen delivery for local metabolic demand - and this releases lactic acid as cells switch to anaerobic metabolism. Recent discoveries demonstrate that sensory neurons innervating the heart are richly endowed with an ion channel that is opened by, and perfectly tuned for, the lactic acid released by muscle ischemia.