RESUMO
The study was designed to examine the effect of oleanolic acid on cauda epididymal sperm motion using a computer-aided sperm analysis system and to elucidate the relationship between sperm motion and fertility, as a tool for contraceptive studies. Oleanolic acid-polyvinylpyrrollidone suspension was orally administered to adult male Wistar rats for 30 days, followed by a 14-day drug withdrawal from half of the rats in the group. Control rats received only polyvinylpyrrollidone. All males were mated with untreated females. Treated males failed to impregnate females, whereas control and oleanolic acid withdrawn males achieved 100% pregnancies. Sperm motion analysed on the Sperm Motility Quantifier (SMQ) showed significant differences in linearity (P < 0.001) and wobble (P < 0.01) between control and treated groups. However, the curvilinear velocities were not significantly different (P > 0.05) among all the groups. Sperm motility patterns verified differences among kinematic parameters.
Assuntos
Anticoncepcionais Masculinos/toxicidade , Fertilidade/efeitos dos fármacos , Ácido Oleanólico/toxicidade , Imobilizantes dos Espermatozoides/toxicidade , Motilidade dos Espermatozoides/efeitos dos fármacos , Administração Oral , Animais , Anticoncepcionais Masculinos/administração & dosagem , Feminino , Processamento de Imagem Assistida por Computador , Masculino , Ácido Oleanólico/administração & dosagem , Distribuição Aleatória , Ratos , Ratos Wistar , Recuperação de Função Fisiológica , Imobilizantes dos Espermatozoides/administração & dosagem , Motilidade dos Espermatozoides/fisiologia , Fatores de TempoRESUMO
BACKGROUND: Pure compounds and herbal medicines, had to be administered orally to vervet monkeys for testing. A reliable method was sought to avoid daily gavaging. METHODS: Substances were incorporated into the maize meal based diet of this facility for voluntary consumption. Food intake was monitored. RESULTS: In all but one case, consumption of the treated food was in excess of 90%. In all cases, the purpose of the study was achieved. Habituation was usually only required to the vehicle, and was accomplished by incremental increases to the desired concentration. Aversion was overcome by the addition of a small amount of honey. Plasma concentrations of a compound metabolite could be measured in one case after gavage and food consumption, and were similar for both methods. CONCLUSION: The method enables reliable voluntary consumption of diverse substances. Since stressful gavaging is avoided, it is a valuable contribution to the 3Rs.
Assuntos
Chlorocebus aethiops/fisiologia , Comportamento Alimentar/fisiologia , Preparações Farmacêuticas/administração & dosagem , Preparações de Plantas/administração & dosagem , Administração Oral , Ração Animal , Animais , Chlorocebus aethiops/metabolismo , Formas de Dosagem , Feminino , Masculino , Preparações Farmacêuticas/metabolismo , Preparações de Plantas/farmacocinéticaRESUMO
Nonhuman primates are of interest as models of human physiology to study the effect of multiple pregnancies on birth weight. Reference plasma and red blood cell (RBC) total phospholipids fatty acids were established in nonpregnant breeding female Vervet monkeys. Twenty-three clinically healthy nonpregnant Vervet monkeys (Cercopithecus aethiops), contained in a controlled closed environment and consuming a high carbohydrate diet (68 E%) that contained 20 E% fat and 12 E% protein were sampled for blood during a cross-sectional study. A low intake of omega3 fatty acids was reflected by a high omega6/omega3 ratio (66:1) of the diet. Inverse relations were seen between plasma and RBC total phospholipid fatty acids, 18:2omega6, 20:3omega6, and 20:4omega6, which suggested selective incorporation in membranes. Low levels of 20:5omega3 and 22:6omega3 of plasma and RBC total phospholipids render Vervet monkeys as ideal subjects to study the effect of omega3 fatty acid supplementation on pregnancy outcomes.