RESUMO
The effects of hypoxic, hyperoxic, and hypoxic-hyperoxic preconditioning were examined in the prospective study on narcotized and artificially ventilated rabbits. Under artificial circulation, acute myocardial ischemia was modeled by ligation of anterior descending coronary artery, which was followed by reperfusion. The degree of ventricular arrhythmias was assessed, and the ischemic area was evaluated in percent of the area at risk. Microscopic characterization of the myocardium was employed to assess the cardioprotective effect of hypoxic and/or hyperoxic preconditioning. According to Kruskal-Wallis test, the greatest resistance of the myocardium to ischemic and reperfusion injury was observed after hypoxic-hyperoxic preconditioning (H=42.459; p=0.009). The rabbits subjected to this type of preconditioning demonstrated the least damaged myocardium in comparison with nonconditioned controls.
Assuntos
Precondicionamento Isquêmico Miocárdico/métodos , Traumatismo por Reperfusão Miocárdica/prevenção & controle , Animais , Hipóxia Celular , Masculino , Miocárdio/patologia , Oxigênio/uso terapêutico , CoelhosRESUMO
The review summarizes information from recent literature and results of the authors' own investigations concerning dysbalance of programmed cell death in establishment of a long-term virus persistense. The article discusses molecular mechanisms of apoptosis modulation of immune cellls by persistent viruses.
Assuntos
Apoptose , Linfócitos B/virologia , Linfócitos T/virologia , Viroses/virologia , Fenômenos Fisiológicos Virais , Adaptação Fisiológica , Animais , Linfócitos B/imunologia , Linfócitos B/patologia , Humanos , Linfócitos T/imunologia , Linfócitos T/patologia , Viroses/imunologia , Viroses/patologia , Vírus/imunologia , Vírus/metabolismoRESUMO
The cytogenetic status and activity of regulatory systems for stability of the cell genome were evaluated in patients with chronic viral persistence. Hepatitis B and C viruses damage the chromosome apparatus of peripheral blood lymphocytes. Cytogenetic instability of immunocompetent cells during chronic viral infection was associated with inhibition of DNA excision repair system and dysregulation of apoptosis in target cells.
Assuntos
Apoptose/fisiologia , Instabilidade Cromossômica/genética , Aberrações Cromossômicas , Hepacivirus , Vírus da Hepatite B , Hepatite Viral Humana/fisiopatologia , Linfócitos/fisiologia , Adulto , Apoptose/genética , Feminino , Fluoresceína-5-Isotiocianato , Hepatite Viral Humana/virologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Contagem de CintilaçãoRESUMO
The structure of lymphocyte and erythrocyte plasma membrane in patients with long persistence of tick-borne encephalitis virus was studied using fluorescent probes pyrene and 1-anilinonaphthalene-8-sulfonate. The authors analyze the clear-cut disorders in the membranes of both lymphocytes and erythrocytes in viral tick-borne-encephalitis from the viewpoint of generalized involvement of cell membranes in infectious process.