Efficacy of Two Larvasonic™ Units Against Culex Larvae and Effects on Common Aquatic Nontarget Organisms in Harris County, Texas.

The Larvasonic™ Field Arm Mobile Wetlands Unit and SD-Mini were tested for efficacy against Culex larvae, and effects on aquatic nontarget organisms (NTO). The Field Arm provided 84.61% to 100% control of caged Culex larvae out to 0.91-m distance in shallow ditches and 60.45% control of Culex larvae at 0.61-m without any effects to caged NTO. Slow ditch treatment achieved 77.35% control compared to fast treatment (20.42%), whereas 77.65% control was obtained along edges of a neglected swimming pool, compared to near the middle (23.97%). In bucket tests, the SD-Mini provided >97% control of Culex and 85.35% reduction of immature giant water bugs, which decreased slightly (83.45%) over the monitoring period, which was not significantly different from cannibalistic damselflies (62.80%), with reduction of both being significantly higher than other NTO tested. There was a small (0.37%) reduction of dragonflies (naiads), due to cannibalism. Both Larvasonic units could effectively augment conventional larvicide operations in smaller areas without causing resistance within mosquito populations or harming NTO when used properly.

Thermal fog efficacy tests against mosquitoes in storm drains in Harris County, Texas 2008-09.

In 2008 and 2009, our current thermal fogging methods and materials were evaluated in underground systems against feral and laboratory strains of adult Culex quinquefasciatus. Culex quinquefasciatus adults collected from storm drain systems the night before treatment were tested concurrently with adults from a susceptible laboratory strain (Sebring) in 10 separate tests. During 2008, there were no significant differences in the low mean percent control obtained between any of the feral populations (29.39%) and susceptible (Sebring) populations (56.04%) tested, whereas in 2009, application of mineral oil alone yielded extremely low, but significantly different mean percent control at 0.99% for ferals and 0.01% for Sebrings. During 2009 mineral oil evaluations, 45,677 droplets were collected in storm drains at distances of 99.1, 50.6, 57.9, 67.7, and 109.7 m from the application site, with 99% of the droplets below 3 microm in diam; additionally, we found no significant differences between mean percent control of Sebring and feral mosquito populations using the higher (3.2x) 1:10 application rate of Pyrocide. However, mean percent control between the feral and susceptible strain (Sebring) during 2009 was lower than in 2008 at 16.55% for ferals and 24.43% in Sebrings. Results indicated that control methodologies and/or chemicals used were ineffective at controlling Cx. quinquefasciatus in storm drains using the chosen experimental design. Based upon this information, thermal fog operations were discontinued due to lack of effectiveness.

Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus Mosquito Vectors.

Microbial interactions are an underappreciated force in shaping insect microbiome communities. Although pairwise patterns of symbiont interactions have been identified, we have a poor understanding regarding the scale and the nature of co-occurrence and co-exclusion interactions within the microbiome. To characterize these patterns in mosquitoes, we sequenced the bacterial microbiome of Aedes aegypti, Ae. albopictus, and Culex quinquefasciatus caught in the field or reared in the laboratory and used these data to generate interaction networks. For collections, we used traps that attracted host-seeking or ovipositing female mosquitoes to determine how physiological state affects the microbiome under field conditions. Interestingly, we saw few differences in species richness or microbiome community structure in mosquitoes caught in either trap. Co-occurrence and co-exclusion analysis identified 116 pairwise interactions substantially increasing the list of bacterial interactions observed in mosquitoes. Networks generated from the microbiome of Ae. aegypti often included highly interconnected hub bacteria. There were several instances where co-occurring bacteria co-excluded a third taxa, suggesting the existence of tripartite relationships. Several associations were observed in multiple species or in field and laboratory-reared mosquitoes indicating these associations are robust and not influenced by environmental or host factors. To demonstrate that microbial interactions can influence colonization of the host, we administered symbionts to Ae. aegypti larvae that either possessed or lacked their resident microbiota. We found that the presence of resident microbiota can inhibit colonization of particular bacterial taxa. Our results highlight that microbial interactions in mosquitoes are complex and influence microbiome composition.