RESUMO
Combined NMR and the vacuum dehydration method were used to study the state of water in rat muscle. Comparative studies were carried out on muscles of young and old mature white rats. The spin-lattice relaxation time was measured as a function of water content. The single-exponential behaviour of the relaxation process indicates a normal fast-exchange process between the different fractions. The more elaborate analysis demonstrates the failure of the original fast-exchange model. It is proved that structural changes take place in the macromolecule/ion/bound water system of the muscle even at the beginning of dehydration. All the muscle water seems to be necessary to maintain the normal natural state of the muscle's molecular structure.
Assuntos
Água Corporal , Músculos , Fatores Etários , Animais , Feminino , Espectroscopia de Ressonância Magnética , RatosRESUMO
Gastric application of high doses of colloidal bismuth subcitrate (CBS) stimulates mucosal prostaglandin E2 (PGE2) production, which is considered part of the mechanism by which the drug accelerates peptic ulcer healing. Whether therapeutic, orally administered, doses of CBS cause a sustained stimulation of prostaglandin production is not known. We have, therefore, determined gastric luminal release of PGE2 during 'steady-state' perfusion of the stomach with CBS (10 mg/ml; isotonic mannitol 5 ml/min) and 4 h after the last oral dose of the drug (240 mg b.d.) for 2 weeks (isotonic mannitol 5 ml/min) in 8 healthy volunteers. A significant increase in PGE2 concentrations (712 (409-1076) vs. control 334 (252-655) pg/ml; medians with Q50 ranges; P less than 0.02) and PGE2 output (12.5 (7.3-14.3) vs. control 4.8 (4.1-7.3) ng/15 min; P less than 0.02) occurred during gastric perfusion with CBS. A similar increase in PGE2 concentrations (630 (297-1429) pg/ml; P less than 0.02) and PGE2 output (12.6 (6.4-22.2) ng/15 min; P less than 0.02) was observed following treatment with CBS for 2 weeks. These results suggest that therapeutic doses of CBS cause a sustained stimulation of gastric mucosal PGE2 formation.
Assuntos
Antiulcerosos/farmacologia , Dinoprostona/metabolismo , Mucosa Gástrica/metabolismo , Compostos Organometálicos/farmacologia , Adulto , Antiulcerosos/administração & dosagem , Coloides , Feminino , Ácido Gástrico/metabolismo , Mucosa Gástrica/efeitos dos fármacos , Humanos , Masculino , Pessoa de Meia-Idade , Compostos Organometálicos/administração & dosagem , PerfusãoRESUMO
Ultrastructural changes of the neuromuscular and microvascular systems were investigated in muscle biopsies obtained from patients with rheumatoid arthritis, systemic lupus erythematosus, dermato- and polymyositis and progressive systemic sclerosis. Myofibrillar degeneration, Z-disc streaming, mitochondrial abnormalities, alteration of the T system and sarcoplasmic reticulum were the main ultrastructural alterations observed in biopsy material. At the same time, severe peripheral nerve-fibre lesions were also observed. Thickening of the basement membrane around capillaries was repeatedly demonstrated, above all in rheumatoid arthritis and progressive systemic sclerosis. Immunocomplexes around capillaries and small vessels as well as along the sarcolemmal basement membrane were also often demonstrated.
Assuntos
Capilares/patologia , Doenças do Tecido Conjuntivo/patologia , Músculos/ultraestrutura , Nervos Periféricos/patologia , Adulto , Artrite Reumatoide/patologia , Membrana Basal/imunologia , Membrana Basal/patologia , Membrana Basal/ultraestrutura , Capilares/imunologia , Capilares/ultraestrutura , Dermatomiosite/patologia , Feminino , Humanos , Imunoglobulinas/análise , Lúpus Eritematoso Sistêmico/patologia , Masculino , Microscopia Eletrônica , Pessoa de Meia-Idade , Mitocôndrias Musculares/patologia , Mitocôndrias Musculares/ultraestrutura , Músculos/irrigação sanguínea , Músculos/patologia , Miofibrilas/patologia , Miofibrilas/ultraestrutura , Miosite/patologia , Nervos Periféricos/ultraestrutura , Retículo Sarcoplasmático/patologia , Retículo Sarcoplasmático/ultraestrutura , Escleroderma Sistêmico/patologiaRESUMO
Human interferon-alpha (Hu-IFN alpha) and phorbol myristate acetate (PMA), a direct activator of protein kinase C (PK-C), induce the translocation of protein kinase C from the cytosol to the membrane fraction. By the use of transmission (TEM) and scanning (SEM) electron microscopy we have shown that treatment of human amniotic cells (UAC) with Hu-IFN alpha resulted in profound changes in the shape, volume and ultrastructure of the cells. Most treated cells had enlarged nuclei with marginal condensation of chromatin. Nucleolar segregation, disintegration and clumping of nucleolar components were also observed. The number of interdigitating cell processes decreased and the cell surface microvilli became shortened. Similar ultrastructural alterations were induced by PMA also. All these functional and morphological data strongly support the hypothesis that protein kinase C is a key factor in IFN-mediated cell reactions.
Assuntos
Âmnio/citologia , Interferon Tipo I/farmacologia , Âmnio/metabolismo , Âmnio/ultraestrutura , Transporte Biológico/efeitos dos fármacos , Humanos , Microscopia Eletrônica , Microscopia Eletrônica de Varredura , Proteína Quinase C/metabolismo , Acetato de Tetradecanoilforbol/farmacologiaRESUMO
Cultures of synovial cells from 8 patients with classical or determined rheumatoid arthritis and 3 patients with non-rheumatoid arthrosis were examined electron microscopically. Cells of the monolayer formed upon prolonged incubation of tissue pieces had the conventional ultrastructure of synovial cells. In 2 out of 8 cultures of rheumatoid synovial cells after 14 days of incubation, budding virus-like particles with the external diameter of 100-120 nm were observed. Morphologically these particles were very similar to retroviruses. Isolated particles had a buoyant density in sucrose of 1.15-1.17 g/cm3.
Assuntos
Artrite Reumatoide/microbiologia , Corpos de Inclusão Viral/ultraestrutura , Membrana Sinovial/microbiologia , Células Cultivadas , Centrifugação com Gradiente de Concentração , Humanos , Articulação do Joelho/microbiologia , Microscopia Eletrônica , Osteoartrite/microbiologia , Membrana Sinovial/ultraestrutura , Sinovite/microbiologiaAssuntos
Acetatos , Colágeno/análise , Animais , Birrefringência , Liofilização , Histocitoquímica , Microscopia Eletrônica , Microscopia de Polarização , Ratos , Soluções , Cauda/análiseAssuntos
Envelhecimento , Colágeno/análise , Animais , Histocitoquímica , Microscopia de Interferência , Ratos , CaudaAssuntos
Colágeno , Desnaturação Proteica , Ureia , Animais , Fenômenos Químicos , Físico-Química , Doenças do Colágeno/etiologia , Microscopia de Polarização , Ratos , Cauda , Tendões , Difração de Raios XAssuntos
Artrite Reumatoide/microbiologia , Orthomyxoviridae/isolamento & purificação , Membrana Sinovial/microbiologia , Adulto , Idoso , Formação de Anticorpos , Artrite Reumatoide/patologia , Núcleo Celular , Histocitoquímica , Humanos , Microscopia Eletrônica , Pessoa de Meia-Idade , Paramyxoviridae/isolamento & purificação , Membrana Sinovial/patologiaRESUMO
Nodular type and diffuse perivascular lymphoid cell infiltrates in rheumatoid synovial membranes from 72 patients were examined under the electron microscope. Attempts were made to describe the ultrastructural relations of the cells examined and to classify the different types of cell-to-cell contracts between them. Members of a complex series, composed of gradually differentiating forms of cell contacts beginning from simple membrane adherences and interdigitations and gap junctions; minute filamentous intercellular bridges: subsurface confronting cisternae and desmosome-like structures, are described and suggested as function-dependent morphological representatives of sites of cellular interactions.
Assuntos
Artrite Reumatoide/patologia , Junções Intercelulares/ultraestrutura , Linfócitos/ultraestrutura , Membrana Sinovial/ultraestrutura , Artrite Reumatoide/fisiopatologia , Comunicação Celular , Desmossomos/ultraestrutura , Humanos , Junções Intercelulares/fisiologia , Linfócitos/fisiologia , Macrófagos/fisiologia , Microscopia Eletrônica , Membrana Sinovial/fisiopatologiaRESUMO
The microvascular basement membrane thickness were investigated by electron microscope within skeletal muscle of 17 untreated patients with primary hyperlipoproteinemia. The average of minimal basement membrane thickness of hyperlipidemic patients were significantly different (p < 0.001) from that of the controls (127 +/- 44.6 nm vs 39.9 +/- 12.4 nm). In conclusion, hyperlipoproteinemia without diabetes mellitus might contribute to the damage of the capillary vessel wall.
Assuntos
Angiopatias Diabéticas/patologia , Hiperlipoproteinemias/patologia , Adulto , Membrana Basal/ultraestrutura , Biópsia por Agulha , Feminino , Humanos , Masculino , Microcirculação/ultraestrutura , Microscopia Eletrônica , Pessoa de Meia-Idade , Músculo Esquelético/irrigação sanguíneaRESUMO
The biopsy material from the sural nerve of 8 patients suffering from rheumatoid arthritis and showing, with one exception, positive serological reactions, were studied by means of electron microscopy. All the patients were displaying sensory and sensomotor neuropathy. Besides hypermyelination the damage of Schwann cells, the destruction on unmyelinated fibres and the proliferation and degeneration of the vascular endothelium were observed. No inflammatory signs were seen around the peri- and endoneurial vessels.
Assuntos
Artrite Reumatoide/patologia , Nervos Espinhais/ultraestrutura , Nervo Sural/ultraestrutura , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Nervo Sural/patologiaRESUMO
The results of 3 bone biopsies in patients with Sillence I. type of osteogenesis imperfecta are described. The biopsy material was examined with light microscope histomorphometric and various electron microscopic methods. Histomorphometry showed that the osteoid rim (OS%) and the steroid surface covered by osteoblasts (OB%) was significantly greater, in the same time the relative quantity of osteoid (Vvos%) was significantly less, compared with the normal bone. Electron microscopy showed the broadening and unevenness of the mineralization front. The diameters of the collagen fibers were quite unstable compared with the control. The structure of the newly developed apatit crystals was not different, although their measure was less than of the control. The microanalytical examinations of Ca/P relation of the crystal phase did not show any difference.
Assuntos
Osteogênese Imperfeita/patologia , Adolescente , Adulto , Fatores Etários , Biópsia , Criança , Pré-Escolar , Feminino , Humanos , Hungria/epidemiologia , Masculino , Microscopia Eletrônica , Osteogênese Imperfeita/classificação , Osteogênese Imperfeita/epidemiologia , Estados Unidos/epidemiologiaRESUMO
T-cell colonies formed in liquid or semisolid cultures of stimulated human blood mononuclear cells were investigated under electron microscope. Typical gap junctions with five-layered structure were demonstrated between colony cells embedded in water miscible resin. The overall thickness of the junctional zones was less than 25 nm. The intercellular gap was demonstrated by lanthanum nitrate staining. Close membrane associations were observed after normal embedding with a junctional width of 20-35 nm corresponding to gap junctions. The functional importance of free communication between colony cells is discussed.
Assuntos
Junções Intercelulares/ultraestrutura , Linfócitos T/ultraestrutura , Ensaio de Unidades Formadoras de Colônias , Humanos , Junções Intercelulares/efeitos dos fármacos , Microscopia Eletrônica , Fito-Hemaglutininas/farmacologia , Linfócitos T/efeitos dos fármacosRESUMO
A 49-year-old female presented with intermittent hydrarthrosis affecting the right knee. Numerous calcium pyrophosphate crystals were demonstrated in synovial fluid aspirated from the knee.
Assuntos
Pirofosfato de Cálcio/análise , Difosfatos/análise , Hidrartrose/metabolismo , Articulação do Joelho , Líquido Sinovial/análise , Feminino , Humanos , Pessoa de Meia-IdadeRESUMO
In a 48-year-old man with dysuria and microscopic haematuria, pyelography, cystoscopy and bimanual bladder palpation showed no definite abnormality. Because of continued symptoms, ultrasound scanning of the abdomen was done three months later. The examination revealed a tumour in close relation to the top of the bladder. Malignant cells were aspirated at ultrasonically guided puncture, and a urachus tumour was suspected. For early diagnosis of urachus tumours, ultrasound investigation of vague bladder symptoms is required.