RESUMO
We studied the effectiveness of Xe/O2 mixture inhalation (30% Xe and 70% O2, 20 min for 5 days) in a model of experimental thromboplastin pneumonitis. Inhalation of the studied mixture decreased the intensity of the inflammatory process in the lung tissue assessed by the temperature response of animals, changed lung weight and lung weight coefficient. At acute stage of pneumonitis, an increase in xenon consumption was recorded due to its retention in the gas exchange zone and a natural decrease in oxygen consumption due to partial alveolar/capillary block. The formation of pneumonitis was accompanied by a pronounced procoagulant shift in the regulation system of the aggregate state of blood. The Xe/O2 inhalations ensured physiologically optimal levels of prothrombin and activated partial thromboplastin time against the background of a moderate decrease in fibrinogen level throughout the experiment. At the same time, the activity of the natural anticoagulant antithrombin III increased from day 5 to day 14.
Assuntos
Oxigênio , Pneumonia , Xenônio , Animais , Pneumonia/sangue , Pneumonia/patologia , Masculino , Oxigênio/metabolismo , Xenônio/administração & dosagem , Xenônio/farmacologia , Hemostasia/efeitos dos fármacos , Administração por Inalação , Fibrinogênio/metabolismo , Tempo de Tromboplastina Parcial , Pulmão/efeitos dos fármacos , Pulmão/metabolismo , Antitrombina III/metabolismo , Ratos , Tromboplastina/metabolismo , Protrombina/metabolismo , Consumo de Oxigênio/efeitos dos fármacos , Coagulação Sanguínea/efeitos dos fármacosRESUMO
We studied the effect of an anthocyanin-containing complex from the fruits of S. aucuparia L. on doxorubicin-induced genotoxicity in bone marrow cells of C57BL/6 mice. The complex reduced the genotoxic effect doxorubicin in metaphase plates of bone marrow cells in 24, 48 h, and 10 days after the administration of the cytostatic. The mean number of single fragments and the fraction of cells with gaps and aberrant metaphases also decreased.
Assuntos
Sorbus , Animais , Camundongos , Antocianinas/farmacologia , Frutas , Camundongos Endogâmicos C57BL , Extratos Vegetais/farmacologiaRESUMO
Paclitaxel in a single MTD of 40 mg/kg caused chromosome aberrations and genome changes (polyploidy) in the bone marrow cells of mice early and 3 months after the injection. The quantity of early precursors of erythropoiesis in the bone marrow decreased, as did their proliferative potential irrespective of the animal gender. Injection of paclitaxel in the MTD caused the development of bone marrow hypoplasia during the early period of observation (up to 14 days) and 3 months after injection.
Assuntos
Células da Medula Óssea/efeitos dos fármacos , Genoma/efeitos dos fármacos , Hematopoese/efeitos dos fármacos , Paclitaxel/farmacologia , Animais , Antineoplásicos/farmacologia , Células da Medula Óssea/metabolismo , Aberrações Cromossômicas/induzido quimicamente , Aberrações Cromossômicas/efeitos dos fármacos , Análise Citogenética , Eritropoese/efeitos dos fármacos , Eritropoese/genética , Feminino , Instabilidade Genômica/efeitos dos fármacos , Hematopoese/genética , Masculino , Camundongos , Camundongos Endogâmicos CBA , Testes de MutagenicidadeRESUMO
We studied toxicity of a new Russian radiopharmaceutical Nanocolloid, (99m)Tc-Al2O3. Tests for acute toxicity showed that this agent belongs to a class of moderate-toxicity substances and does not have cumulative properties. The evaluation of subchronic toxicity after subcutaneous injection of this product to rats (0.04, 0.2, and 0.4 ml/kg) and rabbits (0.02 and 0.2 ml/kg) for 7 days did not reveal changes in the general state, temperature, body weight, indices of the peripheral blood and bone marrow, functions of the heart, liver, kidneys, and nervous system, and morphological characteristics of the internal organs in animals. The drug does not produce a local irritant effect.
Assuntos
Compostos Radiofarmacêuticos/efeitos adversos , Óxido de Alumínio/efeitos adversos , Animais , Temperatura Corporal/efeitos dos fármacos , Feminino , Coração/efeitos dos fármacos , Rim/efeitos dos fármacos , Fígado/efeitos dos fármacos , Masculino , Camundongos , Nanopartículas/efeitos adversos , Sistema Nervoso/efeitos dos fármacos , Coelhos , Ratos , Tecnécio/efeitos adversosRESUMO
Intramuscular injections of Relatox in therapeutic and toxic doses to young outbred laboratory rats for 14 days caused no changes in the peripheral blood and bone marrow parameters, serum biochemical parameters, and morphology of the major viscera. In the toxic dose, the drug caused local irritation (inflammation, atrophy, and sclerosis in muscle tissue). Regeneration processes started in muscle tissue 7 days after Relatox withdrawal.
Assuntos
Toxinas Botulínicas Tipo A/toxicidade , Comportamento Exploratório/efeitos dos fármacos , Hemaglutininas/toxicidade , Locomoção/efeitos dos fármacos , Paresia/induzido quimicamente , Animais , Animais não Endogâmicos , Atrofia , Toxinas Botulínicas Tipo A/farmacologia , Combinação de Medicamentos , Edema/induzido quimicamente , Edema/patologia , Feminino , Hemaglutininas/farmacologia , Membro Posterior , Injeções Intramusculares , Masculino , Músculo Esquelético/efeitos dos fármacos , Músculo Esquelético/patologia , Músculo Esquelético/fisiologia , Miofibrilas/efeitos dos fármacos , Miofibrilas/patologia , Tamanho do Órgão/efeitos dos fármacos , Paresia/patologia , Ratos , Regeneração , Maturidade Sexual , Vísceras/efeitos dos fármacos , Redução de Peso/efeitos dos fármacosRESUMO
Gene protective properties of synthetic antioxidant thiophane and the extract of in vitro cultivated roots of Scutellaria baicalensis were studied on the model of Dr. melanogaster larvae genetic structure damage by drugs cisplatin and cyclophosphan (cyclophosphamide). It is established that adding thiophane or Scutellaria baicalensis root extract to a nutrient medium leads to a decrease in amount of Dr. melanogaster recombinants (females bearing recessive yellow and/or singed marker mutations).
Assuntos
Antioxidantes/farmacologia , Mutagênese/efeitos dos fármacos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Scutellaria baicalensis/química , Tiofenos/farmacologia , Animais , Cisplatino/efeitos adversos , Cisplatino/farmacologia , Ciclofosfamida/efeitos adversos , Ciclofosfamida/farmacologia , Drosophila melanogaster , Extratos Vegetais/químicaRESUMO
The effect of root extract of Baikal skullcap (Scutellaria baicalensis) cultivated in vitro, on the gene structure of CBA/CaLac mice bone marrow cells damaged by anticancer drugs paclitaxel and cisplatin has been studied. It is established that the root extract exhibits gene protective property upon both single and chronic administration.
Assuntos
Cariótipo Anormal , Antineoplásicos Fitogênicos/efeitos adversos , Células da Medula Óssea/metabolismo , Cisplatino/efeitos adversos , Paclitaxel/efeitos adversos , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Cariótipo Anormal/induzido quimicamente , Cariótipo Anormal/efeitos dos fármacos , Animais , Antineoplásicos Fitogênicos/farmacologia , Células da Medula Óssea/patologia , Cisplatino/farmacologia , Feminino , Masculino , Camundongos , Paclitaxel/farmacologia , Extratos Vegetais/química , Scutellaria baicalensis/químicaRESUMO
Beginning with the first hours of experiments, cisplatin evoked an increase of chromosomal aberrations in CBA/CaLac bone marrow cells. Significant increase of structural infringements of chromosomes due to chromatid breaks was revealed in metaphase plates of murine bone marrow preparations through 24 h after cisplatin intraperitoneal introduction. In late terms of research (90th day), the high level of aberrations of chromosomes was retained. The most pronounced correction of cisplatin mutagenicity was achieved using a preliminary course of thiophan introduction.