RESUMO
Plants and plant-derived products have a long history in the treatment of sexual disorders. Rauvolfia vomitoria is one of such plant used traditionally for the enhancement of male sexual and reproductive activity. This study was carried out to elucidate the potential activity of R. vomitoria ethanolic extract on sexual behaviour and male reproductive function. Twenty-five male rats were assigned to five groups and orally treated with distilled water (control), sildenafil citrate (standard) and R. vomitoria ethanolic extract (50, 100 and 200 mg/kg BW) for 22 days. Sexual behaviour parameters such as mount latency (ML), intromission latency (IL), ejaculation latency (EL), mount frequency (MF), intromission frequency (IF), ejaculation frequency (IF) and post-ejaculatory interval (PEI) were recorded at day 0, 1, 8, 15 and 22. The reproductive function including reproductive organ weights, testicular histology and sperm parameters was also assessed. Results showed enhancement in sexual behaviour through significant reduction (p < .01) in ML, IL and PEI and significant increase (p < .01) in EL, MF IF and EF. The extract also caused an increase in sperm count, motility and transit. Present findings demonstrate the ability of R. vomitoria ethanolic extract to improve male sexual behaviour and reproductive activity in rats.
Assuntos
Ejaculação/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Rauwolfia/química , Agentes Urológicos/administração & dosagem , Animais , Etanol/química , Feminino , Masculino , Modelos Animais , Casca de Planta/química , Extratos Vegetais/isolamento & purificação , Ratos , Comportamento Sexual Animal/efeitos dos fármacos , Citrato de Sildenafila/administração & dosagem , Contagem de Espermatozoides , Motilidade dos Espermatozoides/efeitos dos fármacos , Testículo/efeitos dos fármacosRESUMO
The effect of the methanolic extract of Alchornea cordifolia leaves on the fertility of senescent male rats was assessed in this study. 40 rats received daily distilled water, testosterone, 200 and 400 mg/kg of extract of Alchornea cordifolia. The reproductive organs weight, the gonadotropins, testosterone and cholesterol level, the sperm parameters, histology of the testes and epididymis were assessed. The weight of testes and prostate (400 mg/kg) significantly increased (p < 0.05) as well as the level of FHS (p < 0.001), LH and testosterone (p < 0.01) at a dose of 400 mg/kg, respectively, while the cholesterol decreased at a dose of 200 mg/kg (p < 0.05) and 400 mg/kg (p < 0.01) respectively. The testes and epididymis were full of spermatozoa particularly at a dose of 400 mg/kg. The sperm count and morphology significantly increased at both doses of 200 mg/kg (p < 0.01; p < 0.001) and 400 mg/kg (p < 0.001; p < 0.01) respectively. The sperm motion (PROG, VAP, VSL, VCL) (p < 0.001), (ALH, BCF) (p < 0.05) increased at a dose of 200 mg/kg and decreased at a dose of 400 mg/ kg. The overall results provide the strong evidence of the fertility potential of the methanolic extract of Alchornea cordifolia leaves in senescent male rats.
Assuntos
Envelhecimento/fisiologia , Euphorbiaceae/química , Fertilidade/efeitos dos fármacos , Extratos Vegetais/administração & dosagem , Animais , Relação Dose-Resposta a Droga , Epididimo/efeitos dos fármacos , Epididimo/fisiologia , Fertilidade/fisiologia , Masculino , Metanol/química , Modelos Animais , Extratos Vegetais/isolamento & purificação , Folhas de Planta/química , Ratos , Ratos Wistar , Contagem de Espermatozoides , Motilidade dos Espermatozoides/efeitos dos fármacos , Motilidade dos Espermatozoides/fisiologia , Espermatozoides/efeitos dos fármacos , Espermatozoides/fisiologia , Testículo/efeitos dos fármacos , Testículo/fisiologiaRESUMO
This study was designed to investigate the acute and subacute toxicity of the aqueous extract from colocasia esculenta (CE) leaves infected by Phytophthora colocsiae (PC) in rats. Toxicity of the aqueous extract was evaluated in both male and female wistar rats after a single administration (Acute model) and during 21 days (subacute model). Acute administration of the extract up to a dose 4000â¯mg/kg did not induce treatment related signs of toxicity or mortality of any rat tested. Therefore, LD50 was estimated to be more than 4000â¯mg/kg. In the subacute treatment, 3 days before the end of treatment, the male rats treated with the infected Colocasia esculenta leaves extract at doses of 800â¯mg/kg presented some signs of toxicity such as spiky hair, breathing, paralysis and death. Hematological analysis revealed a significant decrease (pâ¯<â¯.05) of platelets for all doses in female rats. Biochemical investigations revealed a significant (pâ¯<â¯.01) increase in the serum AST and HDL cholesterol at dose of 800â¯mg/kg in male rats. A significant decrease (pâ¯<â¯.05) in the level of tissue creatinine at a dose of 800â¯mg/kg in treated female rats has also been observed. Histopathological examination revealed adverse effects on the lungs in male rats at dose of 800â¯mg/kg. The present findings suggest that CE infected by PC would be no major health risks at the doses tested but could affect their health at higher doses and repeated administration.