Alterations in quadriceps muscle cellular and molecular properties in adults with moderate knee osteoarthritis.

OBJECTIVE: Quadriceps muscle weakness is common in knee osteoarthritis (OA). While pain, disuse, and atrophy are commonly cited causes for muscle weakness in OA, emerging evidence suggests changes in muscle quality also occur. Alterations in muscle quality are not well understood, but likely include both cellular and morphologic adaptions. The purpose of this study was to conduct the first cellular-level analysis of the vastus lateralis in adults with moderate knee OA. METHODS: Vastus lateralis biopsies were obtained from 24 subjects with moderate knee OA and 15 healthy controls. Quadriceps strength, muscle fiber cross sectional area (CSA), fiber type distribution, extracellular matrix (ECM) content, satellite cell abundance, and profibrotic gene expression were assessed. RESULTS: Relative to controls, quadriceps strength was significantly lower in OA subjects (OA 62.23, 50.67-73.8 Nm vs 91.46, 75.91-107.0 Nm, P = 0.003) despite no difference in fiber CSA. OA subjects had significantly fewer Type I fibers (OA 41.51, 35.56-47.47% vs 53.07, 44.86-61.29%, P = 0.022) and more hybrid IIa/x fibers (OA 24.61, 20.61-28.61% vs 16.4, 11.60-21.20%, P = 0.009). Significantly greater ECM content, lower satellite cell density, and higher profibrotic gene expression was observed with OA, and muscle collagen content was inversely correlated to strength and satellite cell (SC) density. CONCLUSION: Lower quadriceps function with moderate OA may not result from fiber size impairments, but is associated with ECM expansion. Impaired satellite cell density, high profibrotic gene expression, and a slow-to-fast fiber type transition may contribute to reduced muscle quality in OA. These findings can help guide therapeutic interventions to enhance muscle function with OA.

Effect of pressure insole sampling frequency on insole-measured peak force accuracy during running.

Pressure sensing insoles enable us to estimate forces under the feet during activities such as running, which can provide valuable insight into human movement. Pressure insoles also afford the opportunity to collect more data in more representative environments than can be achieved in laboratory studies. One key challenge with real-world use of pressure insoles is limited battery life which restricts the amount of data that can be collected on a single charge. Reducing sampling frequency is one way to prolong battery life, at the cost of decreased measurement accuracy, but this trade-off has not been quantified, which hinders decision-making by researchers and developers. Therefore, we characterized the effect of decreasing sampling frequency on peak force estimates from pressure insoles (Novel Pedar, 100 Hz) across a range of running speeds and slopes. Data were downsampled to 50, 33, 25, 20, 16 and 10 Hz. Force peaks were extracted due to their importance in biomechanical algorithms trained to estimate musculoskeletal forces and were compared with the reference sampling frequency of 100 Hz to compute relative errors. Peak force errors increased exponentially from 0.7% (50 Hz) to 9% (10 Hz). However, peak force errors were < 3% for all sampling frequencies down to 20 Hz. For some pressure insoles, sampling rate is inversely proportional to battery life. Therefore, these findings suggest that battery life could be increased up to 5x at the expense of 3% errors. These results are encouraging for researchers aiming to deploy pressure insoles for remote monitoring or in longitudinal studies.

The effect of real-time gait retraining on hip kinematics, pain and function in subjects with patellofemoral pain syndrome.

BACKGROUND: Patellofemoral pain syndrome (PFPS) is the most common overuse injury in runners. Recent research suggests that hip mechanics play a role in the development of this syndrome. Currently, there are no treatments that directly address the atypical mechanics associated with this injury. OBJECTIVE: The purpose of this study was to determine whether gait retraining using real-time feedback improves hip mechanics and reduces pain in subjects with PFPS. METHODS: Ten runners with PFPS participated in this study. Real-time kinematic feedback of hip adduction (HADD) during stance was provided to the subjects as they ran on a treadmill. Subjects completed a total of eight training sessions. Feedback was gradually removed over the last four sessions. Variables of interest included peak HADD, hip internal rotation (HIR), contralateral pelvic drop, as well as pain on a verbal analogue scale and the lower-extremity function index. We also assessed HADD, HIR and contralateral pelvic drop during a single leg squat. Comparisons of variables of interest were made between the initial, final and 1-month follow-up visit. RESULTS: Following the gait retraining, there was a significant reduction in HADD and contralateral pelvic drop while running. Although not statistically significant, HIR decreased by 23% following gait retraining. The 18% reduction in HADD during a single leg squat was very close to significant. There were also significant improvements in pain and function. Subjects were able to maintain their improvements in running mechanics, pain and function at a 1-month follow-up. An unexpected benefit of the retraining was an 18% and 20% reduction in instantaneous and average vertical load rates, respectively. CONCLUSIONS: Gait retraining in individuals with PFPS resulted in a significant improvement of hip mechanics that was associated with a reduction in pain and improvements in function. These results suggest that interventions for PFPS should focus on addressing the underlying mechanics associated with this injury. The reduction in vertical load rates may be protective for the knee and reduce the risk for other running-related injuries.

A comparison of tibiofemoral and patellofemoral alignment during a neutral and valgus single leg squat: an MRI study.

BACKGROUND: The etiology of anterior knee pain is not well understood. Recently, excessive hip adduction and internal rotation have been cited as possible factors. However, how these altered hip mechanics affect the patellofemoral joint is still unclear. OBJECTIVE: To compare the three-dimensional tibiofemoral and patellofemoral alignment between a neutral squat and one performed with increased hip adduction and internal rotation. We aimed to examine the relationships between the three-dimensional tibiofemoral and patellofemoral alignment during a neutral and valgus squat. Finally, we aimed to determine the relationship between two-dimensional and three-dimensional measures of patellofemoral alignment. METHODS: 10 healthy subjects were recruited for this study. Knee and patellar kinematics in a neutral squat and one performed with hip adduction and internal rotation were measured using a open, upright, magnetic resonance imaging unit. Both single leg squats were performed at 30° of knee flexion. RESULTS: There was a significant correlation between knee external rotation and lateral patellar translation, and between knee abduction and lateral patellar translation. Moderate relationships were found between the 2D and 3D measures but these were not statistically significant. CONCLUSION: The valgus squat resulted in greater knee external rotation in all subjects. Although mean patellar mechanics were not different in the valgus squat, lateral patellar translation increased as knee external rotation increased. Lastly, 2D measures of patellofemoral alignment only provide moderately fair surrogates for 3D measures.