Profiling of smokers and snuffers among young Finnish men - cross-sectional epidemiological study.

OBJECTIVE: The purpose of this study was to get new information from several sources about the background factors of Finnish smokers, snuffers, and dual users. Profiles of young smokers and snuffers were investigated in association with restorative treatment need, oral hygiene, eating habits, physical activity, body mass index (BMI), psychological and socioeconomic factors. MATERIAL AND METHODS: The study group comprised 3420 conscripts. The data were collected from four different sources: a health examination including an oral health screening, a computer-based questionnaire for investigating individual background factors, a psychological test assessing cognitive skills, and the Cooper test. Statistical analyses comprised cross tabulation and binary logistic regression modelling. RESULTS: The odds for smoking were the greatest among those who had DT (Decayed teeth) > 0, used energy drinks or alcohol regularly, or whose parents were divorced. A score of ≥2900 m in the Cooper test, a higher physical exercise level, a higher own education level, and using sports drinks decreased the odds for smoking. The odds for snuffing were higher among those who ran >2500 m in the Cooper test, had a BMI of ≥25, used sports/energy drinks, or exercised regularly, and lower among those who achieved good results in the cognitive test. Using energy/sports drinks or alcohol was positively and a higher education level was negatively associated with dual use. CONCLUSIONS: Along with increasing prevalence of snuffing, heterogeneity is likely among snuffers. Good cognitive skills may prevent from smoking and snuffing.

Early neointimal coverage and vasodilator response following biodegradable polymer sirolimus-eluting vs. durable polymer zotarolimus-eluting stents in patients with acute coronary syndrome ­HATTRICK-OCT trial.

BACKGROUND: Patients at high bleeding risk would benefit from a shorter dual antiplatelet therapy after PCI. Compared to first-generation devices, the design of newer generation drug-eluting stents may facilitate more rapid anatomical and functional healing of stented vessel based on thinner stent platforms, biodegradable/biocompatible polymers and rapid drug elution. METHODS AND RESULTS: Forty-four non-diabetic patients with acute coronary syndrome (ACS) and culprit lesion in the LAD were randomized to receive either biodegradable polymer sirolimus-eluting stent (BP-SES) or durable polymer zotarolimus-eluting stent (DP-ZES). Neointimal strut coverage was examined using optical coherence tomography, and vasodilator response on invasive thermodilution-derived coronary flow reserve (CFR) at 3-month follow-up. The primary endpoints were percent uncovered struts and CFR. A total of 425 cross-sections (4,897 struts) were analyzed in the BP-SES group, and 425 cross-sections (5,467 struts) in the DP-ZES group. The percent uncovered struts was lower in the BP-SES group compared with the DP-ZES group, both at strut level (3.9% vs. 8.9%, respectively, P<0.001), and stent level (3.9 ± 3.2% vs. 8.9 ± 6.9%, respectively, P=0.019). No significant difference was found between the 2 groups regarding CFR (3.0 ± 1.3 vs. 3.2 ± 1.0, respectively, P>0.05). CONCLUSIONS: In non-diabetic patients with ACS, BP-SES provided slightly better stent strut coverage at 3 months compared with DP-ZES, but neither stent was fully covered. No difference in vasodilator response was seen.

Exercise type and volume alter signaling pathways regulating skeletal muscle glucose uptake and protein synthesis.

PURPOSE: The aim of this study was to compare activation of cellular signaling pathways regulating protein synthesis and glucose uptake in skeletal muscle between resistance and endurance exercise. Moreover, the effect of resistance exercise volume was examined. METHODS: Three groups of male volunteers (26 ± 3 years) were examined: 5 × 10 repetition maximum (RM) resistance exercise (RE) with leg press device (5 × 10 RE; n = 8), 10 × 10 RE (n = 11), and endurance exercise (strenuous 50-min walking with extra load on a treadmill; EE; n = 8). Muscle biopsies were obtained from m.vastus lateralis 30 min pre- and post-exercise. RESULTS: Downstream markers of mTORC1, p-p70S6K(Thr421/Ser424) and p-rpS6(Ser240/244), increased more after 10 × 10 RE than after 5 × 10 RE (p < 0.05) and EE (p < 0.01-0.001). Exercise-induced changes in p-IRS-I(Ser636/639) that inhibit IRS-I signaling via negative feedback from hyperactivated mTORC1 signaling were greater (p < 0.05) after 10 × 10 RE compared with 5 × 10 RE and EE. The changes in energy sensor p-AMPKα(Thr172) were greater after 10 × 10 RE and EE (p < 0.05-0.01) than after 5 × 10 RE. A major regulator of glucose uptake in muscle, p-AS160(Thr642), increased more after 10 × 10 RE than after 5 × 10 RE (p < 0.01) and EE (p < 0.05). CONCLUSION: 10 × 10 RE induced greater activation of important signaling proteins regulating glucose uptake (p-AS160) and protein synthesis (p-p70S6K, p-rpS6) than 5 × 10 RE and EE. The present findings further suggest that, especially after 10 × 10 RE, IRS-I signaling is downregulated and that AS160 is activated through AMPK signaling pathway.

Acute neuromuscular and metabolic responses to combined strength and endurance loadings: the "order effect" in recreationally endurance trained runners.

The study examined the acute neuromuscular and metabolic responses and recovery (24 and 48 h) to combined strength and endurance sessions (SEs). Recreationally endurance trained men (n = 12) and women (n = 10) performed: endurance running followed immediately by a strength loading (combined endurance and strength session (ES)) and the reverse order (SE). Maximal strength (MVC), countermovement jump height (CMJ), and creatine kinase activity were measured pre-, mid-, post-loading and at 24 and 48 h of recovery. MVC and CMJ were decreased (P < 0.05) at post-ES and SE sessions in men. Only MVC decreased in ES and SE women (P < 0.05). During recovery, no order differences in MVC were observed between sessions in men, but MVC and CMJ remained decreased. During recovery in women, a delayed decrease in CMJ was observed in ES but not in SE (P < 0.01), while MVC returned to baseline at 24 h. Creatine kinase increased (P < 0.05) during both ES and SE and peaked in all groups at 24 h. The present combined ES and SE sessions induced greater neuromuscular fatigue at post in men than in women. The delayed fatigue response in ES women may be an order effect related to muscle damage.

Are skeletal muscle FNDC5 gene expression and irisin release regulated by exercise and related to health?

Recently, contradictory findings have been reported concerning the function of irisin and its precursor gene, skeletal muscle FNDC5, in energy homeostasis, and the associated regulatory role of exercise and PGC-1α. We therefore evaluated whether muscle FNDC5 mRNA and serum irisin are exercise responsive and whether PGC-1α expression is associated with FNDC5 expression. The male subjects in the study performed single exercises: (1) 1 h low-intensity aerobic exercise (AE) (middle-aged, n = 17), (2) a heavy-intensity resistance exercise (RE) bout (young n = 10, older n = 11) (27 vs. 62 years), (3) long-term 21 weeks endurance exercise (EE) training alone (twice a week, middle-aged, n = 9), or (4) combined EE and RE training (both twice a week, middle-aged, n = 9). Skeletal muscle mRNA expression was analysed by quantitative PCR and serum irisin by ELISA. No significant changes were observed in skeletal muscle PGC-1α, FNDC5 and serum irisin after AE, EE training or combined EE + RE training. However, a single RE bout increased PGC-1α by 4-fold in young and by 2-fold in older men, while FNDC5 mRNA only increased in young men post-RE, by 1.4-fold. Changes in PGC-1α or serum irisin were not consistently accompanied by changes in FNDC5. In conclusion, for the most part, neither longer-term nor single exercise markedly increases skeletal muscle FNDC5 expression or serum irisin. Therefore their changes in response to exercise are probably random and not consistent excluding the confirmation of any definitive link between exercise and FNDC5 expression and irisin release in humans. Moreover, irisin and FNDC5 were not associated with glucose tolerance and being overweight, or with metabolic disturbances, respectively. Finally, factor(s) other than PGC-1α and transcription may regulate FNDC5 expression.

Variable resistance training promotes greater fatigue resistance but not hypertrophy versus constant resistance training.

Loading using variable resistance devices, where the external resistance changes in line with the force:angle relationship, has been shown to cause greater acute neuromuscular fatigue and larger serum hormone responses. This may indicate a greater potential for adaptation during long-term training. Twelve (constant resistance group) and 11 (variable resistance group) men completed 20 weeks of resistance training with 10 men as non-training controls. Training-induced adaptations were assessed by bilateral leg press one repetition maximum, a repetition to failure test using 75 % 1RM, lower limb lean mass and vastus lateralis cross-sectional area. Only the variable resistance training group improved the total number of repetitions (41 ± 46 %) and volume load (52 ± 37 %) during the repetition to failure test (P < 0.05). Similar improvements in maximum strength and hypertrophy of the lower limbs were observed in both training groups. Also, constant and variable resistance 5 × 10RM leg press loadings were performed before and after training in a crossover design. Acute loading-induced responses were assessed by concentric and isometric force, serum hormone concentrations and phosphorylation of intramuscular signalling proteins (0-30 min post-loading). Greater acute decreases in force (P < 0.05-0.01), and greater increases in serum testosterone and cortisol concentration (P < 0.05) and ERK 1/2 phosphorylation (P < 0.05) were observed following variable resistance loadings before and after training. Greater training-induced improvements in fatigue resistance occurred in the variable resistance training group, which may be due to greater acute fatigue and physiological responses during variable versus constant resistance loadings.

Acute neuromuscular and endocrine responses and recovery to single-session combined endurance and strength loadings: "order effect" in untrained young men.

The purpose of this study was to investigate acute neuromuscular and endocrine responses and recovery to a single session of combined endurance and strength loading using 2 loading orders. Forty-two men were demographically matched to perform a single session of combined endurance + strength (E + S) or strength + endurance (S + E) loading. The strength loading was conducted on a leg press and included sets of power, maximal strength, and hypertrophic loads with an overall duration of 30 minutes. The endurance loading was conducted on a bike ergometer and performed by continuous cycling over 30 minutes at 65% of subject's individual maximal watts. Both loading conditions led to significant acute reductions in maximal force production (E + S: -27%, p < 0.001; S + E: -22%, p < 0.001), rapid force produced in 500 milliseconds (E + S: -26%, p < 0.001; S + E: -18%, p < 0.001), and countermovement jump height (E + S: -15%, p < 0.001; S + E: -12%, p < 0.001), whereas no significant differences between the 2 loadings were observed. Maximal and explosive force production recovered after 48 hours after both loading conditions. Whereas no significant acute responses were found in concentrations of serum testosterone (T) and thyroid-stimulating hormone in the 2 loading conditions, concentrations of T were significantly reduced in E + S during recovery at 24 hours (-13%, p < 0.05) and 48 hours (-11%, p = 0.068), but not in S + E, and concentrations of thyroid-stimulating hormone significantly reduced after both loading conditions (24 hours: E + S, -32%, p < 0.001; S + E, -25%, p < 0.01; 48 hours: E + S, -25%, p < 0.001; S + E, -18%, p < 0.01). The loading conditions in this study showed that neuromuscular performance recovered already within 2 days, whereas endocrine function, observed particularly by decreased concentrations in serum T after the E + S loading order, remained altered still after 48 hours of recovery. These results emphasize the different needs for recovery after 2 loading orders.

Changes in body composition, hormonal status, and physical fitness in 11-, 13-, and 15-year-old Finnish regional youth soccer players during a two-year follow-up.

Vänttinen, T, Blomqvist, M, Nyman, K, and Häkkinen, K. Changes in body composition, hormonal status, and physical fitness in 11-, 13-, and 15-year-old Finnish regional youth soccer players during a two-year follow-up. J Strength Cond Res 25(12): 3342-3351, 2011-The purpose of this study was to examine the changes in body composition, hormonal status, and physical fitness in 10.8 ± 0.3-year-old (n = 13), 12.7 ± 0.2-year-old (n = 14), and 14.7 ± 0.3-year-old (n = 12) Finnish regional youth soccer players during a 2-year monitoring period and to compare physical fitness characteristics of soccer players with those of age-matched controls (10.7 ± 0.3 years, n = 13; 14.7 ± 0.3 years, n = 10) not participating in soccer. Body composition was measured in terms of height, weight, muscle mass, percentage of body fat, and lean body weight of trunk, legs, and arms. Hormonal status was monitored by concentrations of serum testosterone and cortisol. Physical fitness was measured in terms of sprinting speed, agility, isometric maximal strength (leg extensors, abdominal, back, grip), explosive strength, and endurance. Age-related development was detected in all other measured variables except in the percentage of body fat. The results showed that the physical fitness of regional soccer players was better than that of the control groups in all age groups, especially in cardiovascular endurance (p < 0.01-0.001) and in agility (p < 0.01-0.001). In conclusion, playing in a regional level soccer team seems to provide training adaptation, which is beyond normal development and which in all likelihood leads to positive health effects over a prolonged period of time.

Recovery after heavy resistance exercise and skeletal muscle androgen receptor and insulin-like growth factor-I isoform expression in strength trained men.

The effects of heavy resistance exercise on skeletal muscle androgen receptor (AR) protein concentration and mRNAs of AR, insulin-like growth factor-I (IGF)-IEa, and mechano growth factor (MGF) expression were examined from biopsies of vastus lateralis (VL) muscle before and 48 hours after heavy resistance exercise (5 × 10 repetition maximum [RM] leg press and 4 × 10RM squats) in 8 adult strength trained men. The present exercise induced an acute decrease in maximal isometric force and increased serum total testosterone (T) and free testosterone (FT) concentrations. During 2 recovery days, maximal isometric force and subjective perception of physical fitness remained significantly lowered, whereas serum creatine kinase activity, subjective muscle soreness, and muscle swelling (i.e., thickness of VL by ultrasound) were significantly increased compared to pre-exercise values. Subjective perception of physical fitness was followed up to 7 days, and by 6 days postexercise, it was elevated above the pre-exercise level. Basal T and FT concentrations remained unaltered after the exercise. No statistically significant changes were observed in AR protein or mRNA expression, but IGF-IEa (p < 0.05) and MGF (p < 0.05) mRNA expression were increased compared to pre-exercise levels. These findings indicate that IGF-IEa and MGF responses may be related to acute regenerative processes in muscle because of exercise and may contribute to muscular adaptation to resistance exercise. Subjective perception of physical fitness suggests that recovery over a pre-exercise level of the present type of heavy resistance exercise can take approximately 6 days.

Clinical and laboratory risk factors of thrombotic complications after pacemaker implantation: a prospective study.

AIMS: Venous lesions, including obstruction and thromboembolism (VTE), are not uncommon after pacemaker implantation. The purpose of this prospective study was to assess the role of various patient and procedure-related risk factors in the development of these complications. METHODS AND RESULTS: A prospective venography-based study of 150 consecutive pacemaker implantations with a 6-month follow-up was conducted. Current case-control study included all cases (n = 47) with a new venous lesion, and their matched controls. Several surgical and technical factors, i.e. lead burden, choice of venous access, operator experience and procedure duration, as well as patient-related classic risk factors of VTE were assessed. Plasma markers of coagulation and endothelial activation [prothrombin fragment 1 + 2 (F1 + 2), D-dimer (DD), von Willebrand factor (vWF), thrombomodulin (Tm)] were used to evaluate the extent of acute surgical trauma. All cases with venous lesions were also screened for thrombophilia. None of the procedure-related variables were predictive of VTE. Mean levels of vWF, F1 + 2 and DD increased significantly (P < 0.001) and equally in both cases and controls. No single clinical factor predicted venous lesions, but significant (P < 0.05) clustering of classic clinical VTE risk factors was seen among the cases. Thrombophilia was overrepresented in patients with symptomatic pulmonary embolism (2/5, 40%). CONCLUSION: Pacemaker implantation induces a transient hypercoagulable state, but its degree does not predict subsequent venous thromboembolism, and neither did the grade of endothelial damage as reflected by plasma markers. The aetiology of these lesions seems to be multifactorial, and clustering of classic thrombotic risk factors plays a role in the pathogenesis.

Effects of combined strength and endurance training on treadmill load carrying walking performance in aging men.

The present study examined the effects of twice weekly total body strength training (ST), endurance cycling (ET), and combined ST and ET (2+2 times a week) (SET) training on the load carrying walking test performance on the treadmill (TM) and changes in neuromuscular and endurance performance during a 21-week training period in aging men. Forty healthy men (54.8+/-8.0 years) were divided into 3 training groups (ET n=9, ST n=11, SET n=11) and a control group (C, n=9). Peak oxygen uptake (VO2peak), heart rate, and blood lactate concentration were measured before and after a 21-week training program using a graded TM and maximal incremental bicycle ergometer (BE) tests. Isometric forces, vertical jump, and electromyographic activity of leg extensor and/or forearm flexor (F) muscles were measured before and after training and the TM tests. Increases of 20-21% in strength and of 7-12% in cycling BE VO2peak occurred in the training groups, whereas the changes of C remained minor. VO2peak was associated, both before and after training, with TM exercise time in all groups (from r=0.65, p=0.030 to r=0.93, p<0.001). Only SET showed a significant training-induced increase (p=0.011) in exercise time of the TM walking with no significant increase in TM VO2peak. The present data suggest that in older men ET and SET induced specific increases in BE VO2peak and ST and SET in strength. However, only SET increased walking exercise time indicating improved load carrying walking performance because of large individual differences in the magnitude of the development of either strength or endurance capacities.

Effects of aerobic and strength training on aerobic capacity, muscle strength, and gene expression of lymphomonocytes in patients with stable CAD.

This study examined the effectiveness, suitability, and safety of a mixed interval-type aerobic and strength training program (MIAST) on physical fitness in patients with stable coronary artery disease (CAD) without history of myocardial infarction (MI). Twenty-three patients with stable CAD were randomly assigned to a MIAST (n = 12; mean age 58.6 years) or control (n = 11; 63.3 years) group. The MIAST group participated in the progressive training program twice a week for 21 weeks. Peak oxygen uptake (VO2peak), workload, and exercise time were measured as were maximal muscle strength, serum lipids, glucose concentration, and the cross-sectional area (CSA) of knee extensors. The safety and suitability of the program were assessed by wireless electrocardiogram (ECG) monitoring and exercise diaries. VO2peak (6.9%; P < 0.05) and exercise time (11.2%; P < 0.05) improved significantly after 12 weeks of training in the MIAST group compared to the control group. Muscle strength (19.9%; P < 0.05) and CSA (2.2%; P < 0.05) increased, and serum lipids and blood glucose tended to decrease after the training. The successful training program (increase in maximal oxygen uptake) increased the gene expression of oxygen metabolism and decreased the gene expression of inflammation pathways in lymphomonocytes. The MIAST program, including interval-type aerobic and strength training, was safe, did not cause any adverse effects, and led to significant improvements in physical fitness in patients with stable CAD.

Body composition, fitness, and metabolic health during strength and endurance training and their combination in middle-aged and older women.

In this study adaptations in body composition, physical fitness and metabolic health were examined during 21 weeks of endurance and/or strength training in 39- to 64-year-old healthy women. Subjects (n = 62) were randomized into endurance training (E), strength training (S), combined strength and endurance training (SE), or control groups (C). S and E trained 2 and SE 2 + 2 times in a week. Muscle strength and maximal oxygen uptake (VO(2)max) were measured. Leg extension strength increased 9 +/- 8% in S (P < 0.001), 12 +/- 8% in SE (P < 0.001) and 3 +/- 4% in E (P = 0.036), and isometric bench press 20% only in both S and SE (P < 0.001). VO(2)max increased 23 +/- 18% in E and 16 +/- 12% in SE (both P < 0.001). The changes in the total body fat (dual X-ray absorptiometry) did not differ between groups, but significant decreases were observed in E (-5.9%, P = 0.022) and SE (-4.8%, P = 0.005). Lean mass of the legs increased 2.2-2.9% (P = 0.004-0.010) in S, SE and E. There were no differences between the groups in the changes in blood lipids, blood pressure or serum glucose and insulin. Total cholesterol and low-density lipoprotein cholesterol decreased and high-density lipoprotein cholesterol increased in E. Both S and SE showed small decreases in serum fasting insulin. Both endurance and strength training and their combination led to expected training-specific improvements in physical fitness, without interference in fitness or muscle mass development. All training methods led to increases in lean body mass, but decreases in body fat and modest improvements in metabolic risk factors were more evident with aerobic training than strength training.

Safety of diagnostic coronary angiography during uninterrupted therapeutic warfarin treatment.

Long-term warfarin therapy is assumed to increase bleeding and access site complications after coronary angiography and it is often recommended to postpone invasive procedures to reach international normalized ratio (INR) levels <1.8. To assess the safety and feasibility of diagnostic coronary angiography during uninterrupted warfarin therapy, we retrospectively analyzed all consecutive patients (n = 258) on warfarin therapy referred for diagnostic coronary angiography in 2 centers with long experience in uninterrupted warfarin therapy during coronary angiography and in 1 center with a policy of preprocedural warfarin pause. An age- and gender-matched control group (n = 258) with similar disease presentation (unstable or stable symptoms) was collected from each center. Radial access was used in 56% of patients in the warfarin group and in 60% of controls (p = 0.21). There was no difference in access site and bleeding complications (1.9% vs 1.6%) or major adverse cardiovascular and cerebrovascular events (0.4% vs 0.8%) between the warfarin group and their controls. Warfarin was interrupted in 80 patients (31%), and bridging therapy was used in 24 of these patients (30%). INR levels were higher in the uninterrupted warfarin group (2.3 vs 1.9, p <0.001), but the incidence of access site complications was not higher (1.7%) than in patients (n = 80) with a warfarin pause (2.5%) or in patients with pause and bridging therapy (8.3%). Need for blood transfusions (n = 2) occurred only in patients with bridging therapy. Access site complications were more common in the 22 patients with supratherapeutic anticoagulation (INR >3) than in patients with therapeutic periprocedural INR (9.1% vs 1.5%, p <0.05). In conclusion, a simple strategy of performing coronary angiography during uninterrupted therapeutic warfarin anticoagulation is a tempting alternative to bridging therapy and is likely to lead to considerable cost savings.

Body composition and fitness during strength and/or endurance training in older men.

PURPOSE: This study examined adaptations in body composition and physical fitness during a 21-wk strength and/or endurance training period in 40- to 65-yr-old men. We also compared the usefulness of different methods for the analysis of body composition to detect training-induced adaptations. METHODS: Fifty-three men were randomized into the endurance training (E: N = 14), strength training (S: N = 13), combined strength and endurance training (SE: N = 15), or control (C: N = 11) groups. S and E trained 2 and SE 2 x 2 times a week for strength and endurance. RESULTS: Percentage of fat (fat%) decreased (5-8%) similarly in all training groups. Fat% measured by DXA at baseline and its change correlated with those recorded by bioimpedance (r = 0.90 and 0.66), skinfolds (r = 0.80 and 0.78), and waistline (r = 0.84 and 0.74). Lean mass in legs (DXA) increased only in S (2.0 +/- 1.5%, P < 0.001), but the thickness of vastus lateralis and intermedius measured by ultrasound increased (7-11%) in all training groups, and that of triceps brachii increased in S (22%) and SE (20%). Maximal concentric force increased significantly in S, SE, and E (by 22, 23, and 7%), and maximal oxygen uptake increased in both E (11%) and SE (11%). CONCLUSIONS: Waist circumference and skinfold thickness seem to reasonably assess changes in percent body fat during training. However, only DXA was capable to separate small differences between the groups in training-induced changes in lean body mass. Combined strength and endurance training is of greater value than either alone in optimizing body composition or improving physical fitness in older men.

Fitness, body composition and blood lipids following 3 concurrent strength and endurance training modes.

This study investigated changes in physical fitness, body composition, and blood lipid profile following 24 weeks of 3 volume-equated concurrent strength and endurance training protocols. Physically active, healthy male and female participants (aged 18-40 years) performed strength and endurance sessions on different days (DD; men, n = 21; women, n = 18) or in the same session with endurance preceding strength (ES; men, n = 16; women, n = 15) or vice versa (SE; men, n = 18; women, n = 14). The training volume was matched in all groups. Maximal leg press strength (1-repetition maximum (1RM)) and endurance performance (maximal oxygen consumption during cycling), body composition (dual-energy X-ray absorptiometry), and blood lipids were measured. 1RM and maximal oxygen consumption increased in all groups in men (12%-17%, p < 0.001; and 7%-18%, p < 0.05-0.001, respectively) and women (13%-21%, p < 0.01-0.001; and 10%-25%, p < 0.01-0.001, respectively). Maximal oxygen consumption increased more in DD vs. ES and SE both in men (p = 0.003-0.008) and women (p = 0.008-0.009). Total body lean mass increased in all groups (3%-5%, p < 0.01-0.001). Only DD led to decreased total body fat (men, -14% ± 15%, p < 0.001; women, -13% ± 14%, p = 0.009) and abdominal-region fat (men, -18% ± 14%, p = 0.003; women, -17% ± 15%, p = 0.003). Changes in blood lipids were correlated with changes in abdominal-region fat in the entire group (r = 0.283, p = 0.005) and in DD (r = 0.550, p = 0.001). In conclusion, all modes resulted in increased physical fitness and lean mass, while only DD led to decreases in fat mass. Same-session SE and ES combined training is effective in improving physical fitness while volume-equated, but more frequent DD training may be more suitable for optimizing body composition and may be possibly useful in early prevention of cardiovascular and metabolic diseases.

The Effects of Intensive Weight Reduction on Body Composition and Serum Hormones in Female Fitness Competitors.

Worries about the potential negative consequences of popular fat loss regimens for aesthetic purposes in normal weight females have been surfacing in the media. However, longitudinal studies investigating these kinds of diets are lacking. The purpose of the present study was to investigate the effects of a 4-month fat-loss diet in normal weight females competing in fitness-sport. In total 50 participants finished the study with 27 females (27.2 ± 4.1 years) dieting for a competition and 23 (27.7 ± 3.7 years) acting as weight-stable controls. The energy deficit of the diet group was achieved by reducing carbohydrate intake and increasing aerobic exercise while maintaining a high level of protein intake and resistance training in addition to moderate fat intake. The diet led to a ~12% decrease in body weight (P < 0.001) and a ~35-50% decrease in fat mass (DXA, bioimpedance, skinfolds, P < 0.001) whereas the control group maintained their body and fat mass (diet × group interaction P < 0.001). A small decrease in lean mass (bioimpedance and skinfolds) and in vastus lateralis muscle cross-sectional area (ultrasound) were observed in diet (P < 0.05), whereas other results were unaltered (DXA: lean mass, ultrasound: triceps brachii thickness). The hormonal system was altered during the diet with decreased serum concentrations of leptin, triiodothyronine (T3), testosterone (P < 0.001), and estradiol (P < 0.01) coinciding with an increased incidence of menstrual irregularities (P < 0.05). Body weight and all hormones except T3 and testosterone returned to baseline during a 3-4 month recovery period including increased energy intake and decreased levels aerobic exercise. This study shows for the first time that most of the hormonal changes after a 35-50% decrease in body fat in previously normal-weight females can recover within 3-4 months of increased energy intake.

Effects of morning versus evening combined strength and endurance training on physical performance, muscle hypertrophy, and serum hormone concentrations.

This study investigated the effects of 24 weeks of morning versus evening same-session combined strength (S) and endurance (E) training on physical performance, muscle hypertrophy, and resting serum testosterone and cortisol diurnal concentrations. Forty-two young men were matched and assigned to a morning (m) or evening (e) E + S or S + E group (mE + S, n = 9; mS + E, n = 9; eE + S, n = 12; and eS + E, n = 12). Participants were tested for dynamic leg press 1-repetition maximum (1RM) and time to exhaustion (Texh) during an incremental cycle ergometer test both in the morning and evening, cross-sectional area (CSA) of vastus lateralis and diurnal serum testosterone and cortisol concentrations (0730 h; 0930 h; 1630 h; 1830 h). All groups similarly increased 1RM in the morning (14%-19%; p < 0.001) and evening (18%-24%; p < 0.001). CSA increased in all groups by week 24 (12%-20%, p < 0.01); however, during the training weeks 13-24 the evening groups gained more muscle mass (time-of-day main effect; p < 0.05). Texh increased in all groups in the morning (16%-28%; p < 0.01) and evening (18%-27%; p < 0.001), however, a main effect for the exercise order, in favor of E + S, was observed on both testing times (p < 0.051). Diurnal rhythms in testosterone and cortisol remained statistically unaltered by the training order or time. The present results indicate that combined strength and endurance training in the evening may lead to larger gains in muscle mass, while the E + S training order might be more beneficial for endurance performance development. However, training order and time seem to influence the magnitude of adaptations only when the training period exceeded 12 weeks.

Effects of resistance training on expression of IGF-I splice variants in younger and older men.

Insulin-like growth factor-I (IGF-I) and its splice variants Insulin-like growth factor-I isoform Ea (IGF-IEa) and mechano growth factor (MGF) may play an important role in muscular adaptations to resistance training (RT) that may be modulated by ageing. It has been suggested that IGF-I induces cellular responses via AKT8 virus oncogene cellular homolog (Akt) and Extracellular signal-regulated kinase (Erk) signalling pathways. Therefore, resistance exercise-induced changes in skeletal muscle IGF-IEa and MGF messenger ribonucleic acid (mRNA), and MGF, Erk1/2, Akt and p70S6K protein expression were investigated before and after 21 weeks of RT in younger (YM, 20-34 yrs., n = 7) and older men (OM, 51-71 yrs., n = 10). Experimental resistance exercises (RE) of 5 × 10 repetition maximum leg presses were performed pre- and post-RT. Muscle biopsies were obtained before and 48 h after REs, to study the late response to muscle loading. The muscle proteins or mRNAs of interest were not systematically influenced by the REs or RT, except for MGF mRNA expression which was increased (p < .01) following RE before RT in OM. No differences were observed between YM and OM in any variables. This study demonstrated that basal levels or RE-induced responses in skeletal muscle MGF, Erk1/2, Akt and p70S6K protein levels or IGF-IEa and MGF mRNA expression did not differ between YM and OM, nor change systematically due to RT. Thus, ageing appears not to effect expression of the present signalling molecules involved in skeletal muscle hypertrophy.

Combined strength and endurance session order: differences in force production and oxygen uptake.

PURPOSE: To examine acute responses of force production and oxygen uptake to combined strength (S) and endurance-running (E) loading sessions in which the order of exercises is reversed (ES vs SE). METHODS: This crossover study design included recreationally endurance-trained men and women (age 21-45 y; n=12 men, 10 women) who performed ES and SE loadings. Force production of the lower extremities including countermovement-jump height (CMJ) and maximal isometric strength (MVC) was measured pre-, mid-, and post-ES and -SE, and ground-reaction forces, ground-reaction times, and running economy were measured during E. RESULTS: A significant decrease in CMJ was observed after combined ES and SE in men (4.5%±7.0% and 6.6%±7.7%, respectively) but not in women (0.2%±8.5% and 1.4%±7.3% in ES and SE). MVC decreased significantly in both men (20.7%±6.1% ES and 19.3%±9.4% SE) and women (12.4%±9.3% ES and 11.6%±12.0% SE). Stride length decreased significantly in ES and SE men, but not in women. No changes were observed in ground-reaction times during running in men or women. Performing S before E caused greater (P<.01) oxygen uptake during running in both men and women than if E was performed before S, although heart rate and blood lactate were similar between ES and SE. CONCLUSIONS: Performing S before E increased oxygen uptake during E, which is explained, in part, by a decrease in MVC in both men and women, decreased CMJ and stride length in men, and/or an increase in postexercise oxygen consumption.