Assuntos
Antioxidantes/uso terapêutico , Colestase/complicações , Hepatopatias/prevenção & controle , Doença Aguda , Animais , Colestase/cirurgia , Combinação de Medicamentos , Avaliação de Medicamentos , Avaliação Pré-Clínica de Medicamentos , Humanos , Hepatopatias/tratamento farmacológico , Cuidados Pós-Operatórios , Complicações Pós-Operatórias/tratamento farmacológico , Complicações Pós-Operatórias/prevenção & controle , Cuidados Pré-Operatórios , Ratos , Vitaminas/uso terapêuticoRESUMO
The ability of various antibiotics to inhibit superoxide anion(O-2)-mediated formation of adrenochrome from adrenaline and recovery of cytochrome c by xanthine oxidase was studied. In the adrenaline system (pH 10.2), aminoglycosides might be arranged, according to the inhibitory effect, in the following order: monomycin greater than gentamicin greater than kanamycin greater than lincomycin greater than streptomycin. In the xanthine oxidase system (pH 7.8), that order was the following: monomycin greater than gentamicin greater than lincomycin greater than greater than kanamycin. It was suggested that the antibiotic inhibition of the O-2-dependent processes at the essential sites of metabolism and/or the antibiotic involvement into the process of free radical oxidation initiated by O-2 in the cells might be one of the mechanisms of the drug action and toxicity with respect to the host.
Assuntos
Antibacterianos/farmacologia , Superóxidos/antagonistas & inibidores , Adrenocromo/metabolismo , Aminoglicosídeos/farmacologia , Grupo dos Citocromos c/metabolismo , Epinefrina/metabolismo , Radicais Livres , Técnicas In Vitro , Cinética , Oxirredução/efeitos dos fármacos , Soluções , Xantina Oxidase/metabolismoRESUMO
The effect of gentamicin sulfate on the rate of free radical oxidation of adrenalin was studied. Inhibition of the oxidation process at the final concentrations of gentamicin of 0.1-0.2 mg/ml was observed. The mechanism of gentamicin antiradical activity and its role in the cells are discussed.
Assuntos
Epinefrina/antagonistas & inibidores , Gentamicinas/farmacologia , Soluções Tampão , Relação Dose-Resposta a Droga , Epinefrina/metabolismo , Radicais Livres , Técnicas In Vitro , Cinética , Oxirredução/efeitos dos fármacosRESUMO
The activity of superoxide dismutase, glutathione peroxidase, glutathione reductase and glutathione: dehydroascorbate oxidoreductase is lower in the bone marrow than in the liver. The changes in the cellular antioxidative enzymatic system during leg bones fracture and X-ray irradiation are more pronounced in the rat bone marrow, as compared to the liver. The data obtained are in keeping with selective bone-marrow radiosensitivity and with the effect of exogenous superoxide dismutase and glutathione as radioprotectors.