Skeletal muscle intermediate filaments form a stress-transmitting and stress-signaling network.

A fundamental requirement of cells is their ability to transduce and interpret their mechanical environment. This ability contributes to regulation of growth, differentiation and adaptation in many cell types. The intermediate filament (IF) system not only provides passive structural support to the cell, but recent evidence points to IF involvement in active biological processes such as signaling, mechanotransduction and gene regulation. However, the mechanisms that underlie these processes are not well known. Skeletal muscle cells provide a convenient system to understand IF function because the major muscle-specific IF, desmin, is expressed in high abundance and is highly organized. Here, we show that desmin plays both structural and regulatory roles in muscle cells by demonstrating that desmin is required for the maintenance of myofibrillar alignment, nuclear deformation, stress production and JNK-mediated stress sensing. Finite element modeling of the muscle IF system suggests that desmin immediately below the sarcolemma is the most functionally significant. This demonstration of biomechanical integration by the desmin IF system suggests that it plays an active biological role in muscle in addition to its accepted structural role.

Centroid-based maximum intensity projections.

PURPOSE: Maximum intensity projection (MIP) is a three-dimensional visualization technique for tomographic angiograms. While conventional MIPs display contralateral vascular anatomy, this study uses centroid calculations to remove this information. It is necessary to provide accurate, unambiguous vessel depiction and identical projections regardless of slice orientation. METHOD: A mathematical model was formed using parameters from clinical images of the head. The vessel widths from the resulting projections were measured and compared with the model. To test the consistency of the projection process, a clinical image set was reformatted and projections of the same view were compared. RESULTS: The vessel widths were smaller than in the model while varying interpolation and noise. Similar projection views were generated for all slice orientations, but some misalignment was present. CONCLUSION: Vessel width is affected by the ray's path length and interpolation method. Some slight misalignment is present because the reformatting process alters the centroid calculations.