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1.
BMC Vet Res ; 20(1): 372, 2024 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-39160565

RESUMO

BACKGROUND: Neural stem and progenitor cells (NSPCs) from extra-neural origin represent a valuable tool for autologous cell therapy and research in neurogenesis. Identification of proneurogenic biomolecules on NSPCs would improve the success of cell therapies for neurodegenerative diseases. Preliminary data suggested that follicle-stimulating hormone (FSH) might act in this fashion. This study was aimed to elucidate whether FSH promotes development, self-renewal, and is proneurogenic on neurospheres (NS) derived from sheep ovarian cortical cells (OCCs). Two culture strategies were carried out: (a) long-term, 21-days NS culture (control vs. FSH group) with NS morphometric evaluation, gene expression analyses of stemness and lineage markers, and immunolocalization of NSPCs antigens; (b) NS assay to demonstrate FSH actions on self-renewal and differentiation capacity of NS cultured with one of three defined media: M1: positive control with EGF/FGF2; M2: control; and M3: M2 supplemented with FSH. RESULTS: In long-term cultures, FSH increased NS diameters with respect to control group (302.90 ± 25.20 µm vs. 183.20 ± 7.63 on day 9, respectively), upregulated nestin (days 15/21), Sox2 (day 21) and Pax6 (days 15/21) and increased the percentages of cells immunolocalizing these proteins. During NS assays, FSH stimulated NSCPs proliferation, and self-renewal, increasing NS diameters during the two expansion periods and the expression of the neuron precursor transcript DCX during the second one. In the FSH-group there were more frequent cell-bridges among neighbouring NS. CONCLUSIONS: FSH is a proneurogenic hormone that promotes OCC-NSPCs self-renewal and NS development. Future studies will be necessary to support the proneurogenic actions of FSH and its potential use in basic and applied research related to cell therapy.


Assuntos
Hormônio Foliculoestimulante , Animais , Hormônio Foliculoestimulante/farmacologia , Feminino , Ovinos , Ovário/citologia , Células-Tronco Neurais/efeitos dos fármacos , Células Cultivadas , Diferenciação Celular/efeitos dos fármacos
2.
Animals (Basel) ; 14(9)2024 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-38731367

RESUMO

Mesenchymal stem cells (MSCs) are considered a very promising alternative tool in cell therapies and regenerative medicine due to their ease of obtaining from various tissues and their ability to differentiate into different cell types. This manuscript provides a review of current knowledge on the use of MSC-based therapies as an alternative for certain common pathologies in dogs and cats where conventional treatments are ineffective. The aim of this review is to assist clinical veterinarians in making decisions about the suitability of each protocol from a clinical perspective, rather than focusing solely on research. MSC-based therapies have shown promising results in certain pathologies, such as spinal cord injuries, wounds, and skin and eye diseases. However, the effectiveness of these cell therapies can be influenced by a wide array of factors, leading to varying outcomes. Future research will focus on designing protocols and methodologies that allow more precise and effective MSC treatments for each case.

3.
J Am Assoc Lab Anim Sci ; 57(3): 278-281, 2018 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-29784078

RESUMO

Anesthetic protocols may influence adrenal function. Effective methods for modulating stress are desirable to minimize secondary effects during the perioperative period. The aim of this study was to evaluate the effects of the administration of propofol with dexmedetomidine or ketamine on corticoadrenal function and heart and respiratory rates. A random treatment-order design was used: each rabbit received all treatments, with at least 14 d between experiments. Rabbits were assigned to 3 treatment groups (10 per group): group 1, 1 mL normal saline solution intravenously; group 2, propofol (3 mg/kg IV) and dexmedetomidine (0.35 mg/kg IM); and group 3, propofol (3 mg/kg IV) and ketamine (1 mg/kg IV). Dexmedetomidine was injected 15 min prior to propofol administration. Blood samples were obtained before drug administration and at 5, 10, 30, and 60 min and 24 h after injection. Serum cortisol and corticosterone levels were measured by competitive enzyme immunoassay. Serum glucocorticoid concentrations did not change in group 2. However, rabbits in group 3 showed an increase in serum cortisol (at 5-60 min) and corticosterone (at 5-120 min) when compared with all other groups at the corresponding time points. This increase probably reflected both propofol- and ketamine-associated stimulatory effects corticoadrenal function. Respiratory rate decreased in groups 2 and 3 animals, and heart rate decreased in group 2, probably due to sympathetic inhibition by propofol and dexmedetomidine. In conclusion, propofol-ketamine provides suitable cardiorespiratory stability in rabbits but enhances glucocorticoid secretion more than dexmedetomidine-propofol anesthesia. Glucocorticoid levels in anesthetized rabbits should be considered during protocol design to minimize the stress response to surgery and to avoid erroneous data interpretation.


Assuntos
Córtex Suprarrenal/efeitos dos fármacos , Anestésicos/farmacologia , Frequência Cardíaca/efeitos dos fármacos , Coelhos/fisiologia , Taxa Respiratória/efeitos dos fármacos , Anestésicos/administração & dosagem , Animais , Corticosterona/sangue , Dexmedetomidina/administração & dosagem , Dexmedetomidina/farmacologia , Quimioterapia Combinada , Feminino , Coração/efeitos dos fármacos , Hidrocortisona/sangue , Ketamina/administração & dosagem , Ketamina/farmacologia , Ciência dos Animais de Laboratório , Propofol/administração & dosagem , Propofol/farmacologia , Distribuição Aleatória
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